| three antimicrobial metabolites from aspergillus caespitosus. | aspergillus caespitosus nrrl 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against candida albicans atcc 10231 than in broth without sitosterol. subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(s)-acetoxy-6(s)-1,2-trans-epoxypropyl)-2h-pyran-2-one (asperline (1), compound i) and two new metabolites. these were 5,6-dihydro-5(s)-acetoxy-6(s)-(1,2-trans propenyl)-2h-pyran-2-one (compound ii) an ... | 1975 | 1104120 |
| characterization and properties of acid phosphatases with phytase activity produced by aspergillus caespitosus. | high levels of thermostable acid phosphatases were produced by aspergillus caespitosus in culture media supplemented with xylan birchwood or agricultural residues, as carbon sources. the optimal culture conditions for production of phosphatases were 40 degrees c and ph 6.0. extra- and intra-cellular acid phosphatases were purified by chromatography on deae-cellulose, followed by concanavalin a-sepharose affinity separation. both extra- and intra-cellular enzymes were glycoproteins showing 63.0 a ... | 2004 | 14871174 |
| [composite biodegradable materials based on polyhydroxyalkanoate]. | conditions for the processing and mixing of biodegradable polymers at temperatures less than their thermal destruction (130-150 degrees c) using standard equipment have been identified. the structure of the polyhydroxybutyrate/valerate (phb/v) copolymer has been revealed and peculiarities of the crystal phase formation at different monomer ratios have been investigated. it was shown that pure phb with molecular mass 180-270 kda has elastic module approximately 1.2 gpa, strength approximately 25 ... | 2010 | 21261076 |
| production of 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin by aspergillus caespitosus. | aspergillus caepitosus raper and thom cultured on autoclaved cracked, yellow field corn produced 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin which was extracted and then purified by silica gel columns. the physical and spectral properties of the colorless crystals from methylene chloride:hexane were consistent with those published for 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin. yield of high purity 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin was about 140 mg of corn per ... | 1975 | 1147608 |
| effect of carbon source on alkaline phosphatase production and excretion in aspergillus caespitosus. | the effect of several carbon sources on the production of alkaline phosphatase by the thermotolerant aspergillus caespitosus was analysed. the fungus released high levels of alkaline phosphatases into the medium after being cultured for long periods with xylan or industrial residues such as wheat raw and sugar cane bagasse in the culture media. in contrast, the alkaline phosphatase activities were found only intracellulary when the fungus was cultured in glucose-supplemented media. the ph of the ... | 2003 | 12761772 |
| tremorgenic mycotoxins from aspergillus caespitosus. | two tremorgenic mycotoxins were isolated from aspergillus caespitosus, and identified as verruculogen and fumitremorgin b. they were produced at the rate of 172 and 325 mg per kg, respectively, on autoclaved cracked field corn. | 1975 | 1155935 |
| purification and biochemical characterization of a mycelial alkaline phosphatase without dnaase activity produced by aspergillus caespitosus. | biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of a. caespitosus were described. the enzyme was purified 42-fold with 32% recovery by deae-cellulose and concanavalin a-sepharose chromatography. the molar mass estimated by sephacryl s-200 or by 7% sds-page was 138 kda and 71 kda, respectively, indicating a homodimer. temperature and ph optima were 80 degrees c and ph 9.0. this enzyme was highly glycosylated (approximately 74% saccharide content). t ... | 2007 | 17702460 |
| production of the tremorgenic mycotoxins verruculogen and fumitremorgin b by penicillium piscarium westling. | the tremorgenic mycotoxins verruculogen and fumitremorgin b were isolated from penicillium piscarium westling. the coexistence of these tremorgens in culture has previously been reported for one other unrelated fungal species, aspergillus caespitosus raper and thom, and lends further support to the suggestion that the tremorgens have a common biosynthetic origin. | 1977 | 16345234 |
| enzyme inhibitory metabolites from endophytic penicillium citrinum isolated from boswellia sacra. | fungal endophytes establish an important niche within the host plant through the secretion of chemical constituents. isolation of bioactive metabolites could be a vital source for inhibiting the function of enzymes such as α-glucosidase and urease. the present study aimed to elucidate the potential of endophytes associated with boswellia sacra through bioassay-guided isolation and identification of secondary metabolites with enzyme inhibitory ability. endophytic fungal strains viz. penicillium c ... | 2017 | 28220202 |
| production of thermostable invertases by aspergillus caespitosus under submerged or solid state fermentation using agroindustrial residues as carbon source. | the filamentous fungus aspergillus caespitosus was a good producer of intracellular and extracellular invertases under submerged (sbmf) or solid-state fermentation (ssf), using agroindustrial residues, such as wheat bran, as carbon source. the production of extracellular enzyme under ssf at 30°c, for 72h, was enhanced using sr salt solution (1:1, w/v) to humidify the substrate. the extracellular activity under ssf using wheat bran was around 5.5-fold higher than that obtained in sbmf (khanna med ... | 2009 | 24031406 |
| extracellular alkaline phosphatase from the filamentous fungus aspergillus caespitosus: purification and biochemical characterization. | among 30 species of filamentous fungi isolated from brazilian soil, aspergillus caespitosus produced and secreted the highest levels of alkaline phosphatase in culture medium supplemented with xylan. the extracellular alkaline phosphatase was purified by deae-cellulose and concanavalin a-sepharose chromatography. the enzyme was a glycoprotein containing up to 56% sugar with molar mass of 134.8 kda, according to gel filtration in sepharose cl-6b, and 57 kda according to sds-page. nondenaturing el ... | 2003 | 14976719 |