| structure of the acidianus filamentous virus 3 and comparative genomics of related archaeal lipothrixviruses. | four novel filamentous viruses with double-stranded dna genomes, namely, acidianus filamentous virus 3 (afv3), afv6, afv7, and afv8, have been characterized from the hyperthermophilic archaeal genus acidianus, and they are assigned to the betalipothrixvirus genus of the family lipothrixviridae. the structures of the approximately 2-mum-long virions are similar, and one of them, afv3, was studied in detail. it consists of a cylindrical envelope containing globular subunits arranged in a helical f ... | 2008 | 17942536 |
| crystallization and preliminary x-ray diffraction analysis of protein 14 from sulfolobus islandicus filamentous virus (sifv). | a large-scale programme has been embarked upon aiming towards the structural determination of conserved proteins from viruses infecting hyperthermophilic archaea. here, the crystallization of protein 14 from the archaeal virus sifv is reported. this protein, which contains 111 residues (mw 13 465 da), was cloned and expressed in escherichia coli with an n-terminal his(6) tag and purified to homogeneity. the tag was subsequently cleaved and the protein was crystallized using peg 1000 or peg 4000 ... | 2006 | 16946470 |