| application of rdna-pcr amplification and dgge fingerprinting for detection of microbial diversity in a malaysian crude oil. | two culture-independent methods, namely ribosomal dna libraries and denaturing gradient gel electrophoresis (dgge), were adopted to examine the microbial community of a malaysian light crude oil. in this study, both 16s and 18s rdnas were pcr-amplified from bulk dna of crude oil samples, cloned, and sequenced. analyses of restriction fragment length polymorphism (rflp) and phylogenetics clustered the 16s and 18s rdna sequences into seven and six groups, respectively. the ribosomal dna sequences ... | 2008 | 18633276 |