| cloning and sequence analysis of an infectious clone of citrus yellow mosaic virus that can infect sweet orange via agrobacterium-mediated inoculation. | citrus yellow mosaic virus (cymv), a member of the family caulimoviridae, genus badnavirus, causes citrus mosaic disease, a disease that occurs commonly in india. the cymv genome has been cloned and its complete nucleotide sequence determined. its dna genome is 7559 bp in length and contains six putative open reading frames (orfs), all on the plus-strand of the genome and each capable of encoding proteins with a molecular mass of greater than 10 kda. orf 3, the largest orf, encodes a putative po ... | 2001 | 11562547 |
| genomic characterisation of taro bacilliform virus. | taro bacilliform virus (tabv) has been classified as a putative badnavirus based on its non-enveloped, bacilliform virion morphology and transmission by mealybugs. the complete nucleotide sequence of a papua new guinea isolate of tabv has now been determined and comprises 7458 bp. the genome contains four open reading frames (orfs) on the plus-strand that potentially encode proteins of 17, 16, 214 and 13 kda. the size and organisation of tabv orfs 1-3 is similar to that of most other badnaviruse ... | 2003 | 12721801 |
| sodium sulphite yields improved dna of higher stability for pcr detection of citrus yellow mosaic virus from citrus leaves. | citrus yellow mosaic virus (cymv), a non-enveloped bacilliform dna virus causes a severe mosaic disease in sweet oranges in india. cymv is weakly immunogenic, thus serodiagnosis is not a preferred method for its detection. as an alternative a rapid and reliable detection protocol by polymerase chain reaction (pcr) was developed. however, high levels of polyphenolics and tannins in citrus leaves generally interfered with obtaining good quality dna, and thus affected the reliable detection of viru ... | 2003 | 12951224 |
| a multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees. | a multiplex polymerase chain reaction (mpcr) assay was developed to detect six rna and one dna citrus virus: citrus leaf rugose virus (clrv), citrus psorosis virus (cpsv), citrus tatter leaf virus (ctlv), citrus tristeza virus (ctv), citrus variegation virus (cvv), citrus yellow mosaic virus (cymv), and indian citrus ringspot virus (icrsv) from citrus plants. these seven viruses are classified in six different virus genera. degenerate primers were designed based on the respective virus isolate s ... | 2005 | 15951030 |
| construction of infectious clones of double-stranded dna viruses of plants using citrus yellow mosaic virus as an example. | double-stranded dna (dsdna) viruses of plants are believed to be plant pararetroviruses. their genome is replicated by reverse transcription of a larger than unit-length terminally redundant rna transcript of the viral genomic dna using the virus-encoded replicase. in order to produce a cloned, infectious viral genome, the clone must be constructed in a binary vector and be longer than the full, unit-length viral genome. the clone can then be transferred by agrobacterium-assisted inoculation int ... | 2008 | 18370278 |
| development of multiplex polymerase chain reaction assay for simultaneous detection of clostero-, badna- and mandari-viruses along with huanglongbing bacterium in citrus trees. | citrus trees harbor a large number of viral and bacterial pathogens. citrus yellow vein clearing virus (cyvcv), indian citrus ringspot virus (icrsv), citrus yellow mosaic virus (cymv), citrus tristeza virus (ctv) and a bacterium, candidatus liberibacter asiaticus (cla) associated with huanglongbing (hlb) disease, the most prevalent pathogens in citrus orchards of different regions in india and are responsible for debilitating citriculture. for detection of these viral and bacterial pathogens a q ... | 2016 | 27208471 |