| experimentally induced leptospira interrogans serovar autumnalis infections in young swine. | leptospira interrogans serovar autumnalis strain 621 was proven to be infective for swine by intranasal and ocular inoculation and by contact exposure. the course of the disease was that of a chronic asymptomatic infection. throughout the experiment, leptospires were observed in all tissues examined by darkfield microscopy of 10% tissue suspensions and examination by indirect fluorescent antibody technique of frozen tissue sections. infected pigs had increasing antibody titers, as determined by ... | 1979 | 525853 |
| cloning of genes for a hemolytic factor of leptospira interrogans serovar autumnalis strain congo 21-543. | a dna fragment encoding a hemolytic factor was cloned from the parasitic spirochete leptospira interrogans serovar autumnalis strain congo 21-543. initial clones were isolated by screening a genomic library in pbr322 in escherichia coli for hemolytic activity. hemolytic activity was coded by a 4.5 kilobase bamhi-hindiii fragment. southern hybridization with dnas from other strains of leptospira using this gene as a probe showed that dnas from non-parasitic strains failed to hybridize with the pr ... | 1990 | 2077368 |
| leptospirosis in madras--a clinical and serological study. | leptospirosis was confirmed by microscopic agglutination test (mat) and/or elisa in 57 patients admitted to the government general hospital, madras, india, during november and december of 1990 and 1991 with symptomatology suggestive of the disease. fifty (88%) of the 57 cases were males; the mean age of all the cases was 39.6 years (range 17-72). the main clinical features were: fever 100% jaundice 84%, myalgia 82%, acute renal failure 72% and conjunctival suffusion 58%. non-azotemic jaundice oc ... | 1995 | 8713215 |
| leptospira autumnalis isolated from a human case from avadi, india, and the serovar's predominance in local rat and bandicoot populations. | leptospira were successfully isolated from the urine of an indian patient who had been clinically diagnosed as having leptospirosis. in an attempt to determine the source of this infection, 28 rats (rattus rattus) and 58 bandicoots (bandicota bengalensis) living in the vicinity of the patient's home in avadi, a suburban area of the city of chennai (madras), india, were then investigated. each animal was checked for infection by microscopical examination of fresh and stained urine, serological an ... | 2000 | 10983563 |
| identification of the hemolysis-associated protein 1 as a cross-protective immunogen of leptospira interrogans by adenovirus-mediated vaccination. | new vaccine strategies are needed for the prevention of leptospirosis, a widespread human and animal disease caused by pathogenic leptospires. our previous work determined that a protein leptospiral extract conferred cross-protection in a gerbil model of leptospirosis. the 31- to 34-kda protein fraction of leptospira interrogans serovar autumnalis was shown sufficient for this purpose. in the present study, n-terminal sequencing of a 32-kda fraction and southern blotting of genomic dna with corr ... | 2001 | 11598056 |
| analyses of vaccination protocols for leptospira interrogans serovar autumnalis in hamsters. | leptospirosis, caused by leptospira spp., is a zoonotic disease found worldwide. killed whole cell leptospiral vaccines have been used as effective vaccines to elicit specific antibodies for protection. however, the involvement of cytokine responses after vaccination is not well characterized. hamsters were immunized with killed l. interrogans serovar autumnalis before challenge to study cytokine mrna expression levels (interferon [ifn]-gamma, tumor necrosis factor [tnf]-alpha, interleukin [il]- ... | 2008 | 18981522 |
| serodiagnosis of severe leptospirosis: evaluation of elisa based on the recombinant ompl1 or lipl41 antigens of leptospira interrogans serovar autumnalis. | elisa based on the recombinant ompl1 and lipl41 antigens of leptospira interrogans serovar autumnalis strain n2 have been developed, for the serodiagnosis of the severe pulmonary form of leptospirosis on the endemic andaman islands. the recombinant ompl1 and lipl41 were produced using escherichia coli expression systems and then purified before each was evaluated in an igm-elisa. the sera tested came from 224 patients who had the severe form of leptospirosis and pulmonary pneumonitis as major sy ... | 2008 | 19000387 |
| expression and characterization of recombinant leptospiral outer membrane protein lipl32 from leptospira interrogans serovar autumnalis. | leptospira interrogans serovar autumnalis, a causative agent of leptospirosis in thailand, was isolated from a patient for dna extraction and amplification of lipl32 gene by polymerase chain reaction (pcr). the 782 bp pcr product was obtained, which was inserted into pae plasmid with polyhistidine (his6 tag) to construct pae-lipl32. this recombinant plasmid was transfected into e. coli bl21 (de3). his6-lipl32 was purified by ni-nta affinity chromatography. the recombinant protein was used as ant ... | 2009 | 19323048 |
| surface-associated hsp60 chaperonin of leptospira interrogans serovar autumnalis n2 strain as an immunoreactive protein. | the present study has been formulated in order to detect an immunoreactive protein whose identification can play a major role in the early diagnosis of disease. the identified protein will be produced by recombinant methods and used for the recombinant protein based elisa. a comparison was made between the developed method and the gold standard mat test to evaluate the serodiagnosis potential of the protein. the protein profile, immunoblot and maldi-tof analysis was carried out to identify the i ... | 2011 | 21468684 |
| cloning, expression, and homology modeling of groel protein from leptospira interrogans serovar autumnalis strain n2. | leptospirosis is an infectious bacterial disease caused by leptospira species. in this study, we cloned and sequenced the gene encoding the immunodominant protein groel from l. interrogans serovar autumnalis strain n2, which was isolated from the urine of a patient during an outbreak of leptospirosis in chennai, india. this groel gene encodes a protein of 60 kda with a high degree of homology (99% similarity) to those of other leptospiral serovars. recombinant groel was overexpressed in escheric ... | 2011 | 22196358 |