| genome characterization of a flexuous rod-shaped mycovirus, botrytis virus x, reveals high amino acid identity to genes from plant 'potex-like' viruses. | this study reports the molecular characterization of a flexuous rod-shaped mycovirus, botrytis virus x (bvx), infecting the plant-pathogenic fungus, botrytis cinerea. bvx contains a ssrna genome of 6966 nucleotides, and a poly(a) tract at or very near the 3' terminus. computer analysis of the genomic cdna sequence of bvx revealed five potential open reading frames (orfs). orf1 showed significant amino acid sequence identity to the replicase proteins of plant 'potex-like' viruses, including 73% i ... | 2006 | 16172841 |
| viruses of botrytis. | botrytis cinerea (gray mold) is one of the most widespread and destructive fungal diseases of horticultural crops. propagation and dispersal is usually by asexual conidia but the sexual stage (botryotinia fuckeliana (de bary) whetzel) also occurs in nature. dsrnas, indicative of virus infection, are common in b. cinerea, but only four viruses (botrytis virus f (bvf), botrytis virus x (bvx), botrytis cinerea mitovirus 1 (bcmv1), and botrytis porri rna virus) have been sequenced. bvf and bvx are u ... | 2013 | 23498909 |
| recombinant expression of the coat protein of botrytis virus x and development of an immunofluorescence detection method to study its intracellular distribution in botrytis cinerea. | botrytis cinerea is infected by many mycoviruses with varying phenotypical effects on the fungal host, including botrytis virus x (bvx), a mycovirus that has been found in several b. cinerea isolates worldwide with no obvious effects on growth. here we present results from serological and immunofluorescence microscopy (ifm) studies using antiserum raised against the coat protein of bvx expressed in escherichia coli fused to maltose-binding protein. due to the high yield of recombinant protein it ... | 2012 | 22855784 |