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nucleotide sequence and taxonomy of cycas necrotic stunt virus. brief report.cycas necrotic stunt virus (cnsv) is the only well-characterized virus from gymnosperm. cdna segments corresponding to the bipartite genome rnas (rna1, rna2) were synthesized and sequenced. each rna encoded a single polyprotein, flanked by the 5' and 3' non-coding regions (ncr) and followed by a poly (a) tail. the putative polyproteins encoded by rna1 and rna2 had sets of motifs, which were characteristic of viruses in the genus nepovirus. the polyproteins showed higher sequence identities to ar ...200212417954
neurovirulence of six different murine coronavirus jhmv variants for rats.six variant viruses of the jhmv strain of murine coronavirus with large (cl-2, cnsv, dl and ds) or small (sp-4 and jhm-x) s proteins were compared in terms of their relative neurovirulence in weanling lewis rats. inoculation of various doses of the variants revealed that the cl-2 and cnsv were highly virulent and dl and ds were low-virulent, while sp-4 and jhm-x were avirulent. pathological examination of rats infected with variants cl-2, dl and sp-4 showed that the cl-2 and dl induced severe an ...19911656623
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