Publications

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accumulation and persistence of hepatitis a virus in mussels.accumulation and persistence of hepatitis a virus (hav) in the mussel mytilus chilensis was evaluated. under optimal filtration activity of mussels (temperature 12 degrees c, salinity 3%, feeding twice a day with dunaliella marina), hav was concentrated 100-fold from the surrounding water. similar concentrations of hav were reached in the filtration apparatus and in the digestive system (hepatopancreas). hav persisted for about 7 days in mussels. elimination of hav from mussels was slower than e ...19921331305
[a model for demonstrating the accumulation and elimination of phage lambda in mytilus chilensis]. 19862946128
microalgal blooms: a global issue with negative impact in chile.ecological and health problems posed by microalgal blooms (red tides) occurring in the southern part of chile are reviewed. out of the six human illnesses provoked by microalgal toxins, paralytic shellfish poisoning is the most important, because of its high mortality rate and the high levels of phycotoxins found in contaminated molluscs. saxitoxin and its analogues bind to a receptor in the voltage-gated sodium channel of neural membranes. the most important toxin-producer microalgae are alexan ...199810029901
sodium, k, ca, mg, fe, cu, and zn concentrations in molluscs from the magellan strait (chile): their contribution to dietary intake.the concentrations of the essential metals na, k, ca, mg, fe, cu, and zn were determined in 126 specimens of molluscs belonging to five different species: mytilus chilensis, n=47; nacella deaurata, n=65; aulacomya ater, n=4; fissurella picta, n=4; acanthina monodon, n=6, collected from the coastline of the magellan strait. significant differences were obtained among the mean metal concentrations in the mollusc species considered. the contribution to the dietary daily intake of these metals for t ...200516236595
baseline trace metals in bivalve molluscs from the beagle channel, patagonia (argentina).in order to test the aptitude of individuals of mytilus chilensis as biomonitors of heavy metals pollution in seawater, several samples of this mollusk together with surrounding seawater samples were collected along 170 km of the coastal area of the beagle channel (tierra del fuego, argentina) in 2005 and 2007. the study, performed in seven locations strategically selected, involved the determination of cd, cr, cu, ni, pb and zn in seawaters and mollusks by atomic absorption spectrometry (aas) a ...201121544557
high amount of dinophysistoxin-3 in mytilus chilensis collected in seno de reloncaví, chile, during massive human intoxication associated with outbreak of vibrio parahaemolyticus.this study describes the detection of high amount of 7-o-acyl-derivative dinophysistoxin-1 (dinophysistoxin-3) in filter bivalves collected on february 2005 in the seno de reloncaví, puerto montt city, southern chile, in the same period of time where an intoxication episode was associated with the presence of vibrio parahaemolyticus in shellfish. the diarrhetic shellfish poisoning (dsp) mouse bioassay of mussel extract samples, performed as described for regulatory testing, were negative to dsp ...200617077585
simultaneous presence of paralytic and diarrheic shellfish poisoning toxins in mytilus chilensis samples collected in the chiloe island, austral chilean fjords.the study shown here provides the first indisputable evidence that shellfish can be contaminated with paralytic shellfish poisoning (psp) and diarrheic shellfish poisoning (dsp) toxins during the summer season in the southern chilean fjords. quantitative analysis of the simultaneous presence of psp and dsp toxins in mytilus chilensis samples collected in the chiloe island are shown. the high performance liquid chromatography (hplc) analysis with pre-column derivatization method for dsp toxins an ...200415586821
manganese, nickel, selenium and cadmium in molluscs from the magellan strait, chile.the concentrations of manganese, selenium, nickel and cadmium were determined in 112 samples of molluscs belonging to mussels (mytilus chilensis, n = 47) and limpets (nacella deaurata, n = 65), which were collected from the coastline of the magellan strait, chile. four (6.2%) samples of limpets exceeded the maximum limits for cadmium established in europe. limpets showed higher mean manganese, nickel and cadmium concentrations than mussels, whilst the mean selenium concentration in mussels was h ...200415370827
polycyclic aromatic hydrocarbons in sediments and mussels of corral bay, south central chile.pahs were measured in sediments and mussels (mytilus chilensis) from carboneros and puerto claro, located in corral bay, valdivia. according to the ratio of phenanthrene/anthracene and fluoranthene/pyrene concentrations, these sites are medium polluted with pahs originating mainly from pyrolytic sources. fluoranthene was the major component measured in mussels (3.1-390 ng g(-1) dry weight) and sediments (6.9-74.1 ng g(-1) dry weight). in general, mussels were mainly exposed to the dissolved frac ...200414999321
identification of immune-related snps in the transcriptome of mytilus chilensis through high-throughput sequencing.single nucleotide polymorphisms (snps) identified in coding regions represent a useful tool for understanding the immune response against pathogens and stressful environmental conditions. in this study, a snps database was generated from transcripts involved in the innate immune response of the mussel mytilus chilensis. the snps were identified through hemocytes transcriptome sequencing from 18 individuals, and snps mining was performed in 225,336 contigs, yielding 20,306 polymorphisms associate ...201324080470
genetic considerations for mollusk production in aquaculture: current state of knowledge.in 2012, world mollusk production in aquaculture reached a volume of 15,171,000 tons, representing 23% of total aquaculture production and positioning mollusks as the second most important category of aquaculture products (fishes are the first). clams and oysters are the mollusk species with the highest production levels, followed in descending order by mussels, scallops, and abalones. in view of the increasing importance attached to genetic information on aquaculture, which can help with good m ...201425540651
transfection of spermatozoa in bivalve molluscs using naked dna.spermatozoa from the bivalve molluscs mytilus galloprovincialis, mytilus chilensis and chamelea gallina were transfected in vitro using the p-genegrip construct, which encodes green fluorescent protein. the efficiency of transfection after brief incubation was assessed by fluorescence and confocal laser microscopy, and was about 58.5-70.01% in the species used. the foreign gene was principally located in the sperm nuclei, as demonstrated by laser confocal serial sections. in some spermatozoa, mi ...200515774314
the occurrence of domoic acid linked to a toxic diatom bloom in a new potential vector: the tunicate pyura chilensis (piure).the tunicate pyura chilensis (molina, 1782); phylum chordata; subphylum urochordata; class ascidiacea, common local name "piure" or sea squirt; a filter-feeder (plankton and suspended particles) sessile species; may play an important role in monitoring domoic acid (da) the principal toxic component of amnesic shellfish poisoning (asp). significant da concentrations have been determined in tunicate samples, collected during a recent asp outbreak in bahía inglesa, an important scallop (argopecten ...200919505493
distribution and growth of vibrio parahaemolyticus in southern chilean clams (venus antiqua) and blue mussels (mytilus chilensis).we evaluated the distribution and growth of vibrio parahaemolyticus in the inland sea of southern chile, where the world's largest foodborne gastroenteritis outbreak by the pandemic strain o3:k6 occurred in 2005. intertidal samples of mytilus chilensis and venus antiqua were collected around port towns between 41°28's and 43°07's, during april to may 2011 and january to march 2012. we used most probable number real-time polymerase chain reaction (mpn-pcr) for enumeration of the tlh, tdh, and trh ...201525383987
use of cellulolytic marine bacteria for enzymatic pretreatment in microalgal biogas production.in this study, we designed and evaluated a microalgal pretreatment method using cellulolytic bacteria that naturally degrades microalgae in their native habitat. bacterial strains were isolated from each of two mollusk species in a medium containing 1% carboxymethyl cellulose agar. we selected nine bacterial strains that had endoglucanase activity: five strains from mytilus chilensis, a chilean mussel, and four strains from mesodesma donacium, a clam found in the southern pacific. these strains ...201424795376
cadmium bioaccumulation and retention kinetics in the chilean blue mussel mytilus chilensis: seawater and food exposure pathways.the chilean blue mussel (mytilus chilensis, hupe 1854) represents the most important bivalve exploited along the chilean coast and is a major food source for the chilean population. unfortunately, local fish and shellfish farming face severe problems as a result of bioaccumulation of toxic trace metals into shellfishes. blue mussels collected along the chilean coasts contain levels of cd above the regulatory limits for human consumption. in this study, we examined the bioaccumulation, depuration ...201020638735
first evidence of azaspiracids (azas): a family of lipophilic polyether marine toxins in scallops (argopecten purpuratus) and mussels (mytilus chilensis) collected in two regions of chile.azaspiracids are a family of lipophilic polyether marine biotoxins that have caused a number of human intoxication incidents in europe since 1995 following the consumption by consumers of intoxicated shellfish (mytilus edulis). these azaspiracids have now been identified in mussels (mytilus chilensis) and scallops (argopecten purpuratus) from two chilean locations. this is the first report of the occurrence of azaspiracid toxins in these species (mytilus chilensis and argopecten purpuratus) from ...201019852974
application of chemometric studies to metal concentrations in molluscs from the strait of magellan (chile).na, k, ca, mg, fe, cu, zn, mn, se, ni, and cd concentrations were determined in 126 mollusc samples belonging to five different species (mytilus chilensis, n = 47; nacella deaurata, n = 65; aulacomya ater, n = 4; fissurella picta, n = 4; acanthina monodon, n = 6) collected from the coasts of the strait of magellan. the metals analysed presented significant differences between the mean concentrations for the mollusc species considered. factor and discriminant analyses made possible the differenti ...200717375348
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