| molecular identification of bacteria and eukarya inhabiting an antarctic cryoconite hole. | inhabitants of a cryoconite hole formed in the canada glacier in the mcmurdo dry valley region of antarctica have been isolated and identified by small subunit (16s/18s) rdna amplification, cloning, and sequencing. the sequences obtained revealed the presence of members of eight bacterial lineages (acidobacterium, actinobacteria, cyanobacteria, cytophagales, gemmimonas, planctomycetes, proteobacteria, and verrucomicrobia) and metazoan (nematode, tardigrade, and rotifer), truffle (choiromyces), c ... | 2003 | 12768448 |
| gemmatimonas aurantiaca gen. nov., sp. nov., a gram-negative, aerobic, polyphosphate-accumulating micro-organism, the first cultured representative of the new bacterial phylum gemmatimonadetes phyl. nov. | a phylogenetically novel aerobic bacterium was isolated from an anaerobic-aerobic sequential batch reactor operated under enhanced biological phosphorus removal conditions for wastewater treatment. the isolation strategy used targeted slowly growing polyphosphate-accumulating bacteria by combining low-speed centrifugations and prolonged incubation on a low-nutrient medium. the isolate, designated strain t-27t, was a gram-negative, rod-shaped aerobe. cells often appeared to divide by budding repl ... | 2003 | 12892144 |
| bacterial diversity of a soil sample from schirmacher oasis, antarctica. | the bacterial diversity of a soil sample collected in the vicinity of lake zub, schirmacher oasis, antarctica, was determined both by establishing pure colonies of culturable bacteria and by cloning the total 16s rdna of the soil and establishing the phylogeny of the clones. analysis of the 16s rrna gene clones indicated that the bacteria belonged to the classes alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria, gemmatimonas, bacteriodetes, actinobacteria, chloroflexi and chlamydia ... | 2004 | 15559969 |
| bacterial, archaeal and eukaryotic diversity of smooth and pustular microbial mat communities in the hypersaline lagoon of shark bay. | the bacterial, archaeal and eukaryotic populations of nonlithifying mats with pustular and smooth morphology from hamelin pool, shark bay were characterised using small subunit rrna gene analysis and microbial isolation. a highly diverse bacterial population was detected for each mat, with 16s rdna clones related to actinobacteria, bacteroidetes, chloroflexi, cyanobacteria, gemmatimonas, planctomycetes, alphaproteobacteria, gammaproteobacteria, deltaproteobacteria, verrucomicrobia and candidate ... | 2009 | 19200148 |
| effect of gelling agent on colony formation in solid cultivation of microbial community in lake sediment. | since robert koch and colleagues found agar to be an effective gelling agent over a century ago, the pure culture method using agar plates has long been a standard of microbiology. agar is undoubtedly easy to handle and useful for culture of microorganisms, but recent discovery of the ubiquity of microorganisms that cannot be cultured on agar raises a question: is agar really the best agent? in this study, we investigated the effect of two gelling agents, agar and gellan gum, on colony formation ... | 2009 | 19302540 |
| monitoring microbial community structure and succession of an a/o sbr during start-up period using pcr-dgge. | polymerase chain reaction (pcr)-denaturing gradient gel electrophoresis (dgge) protocol was employed for revealing microbial community structure and succession in a sequential anaerobic and aerobic reactor performing enhanced biological phosphorus removal (ebpr) during start-up period. high phosphorus removal was achieved after 15 d. on day 30, phosphorus removal efficiency reached to 83.2% and the start-up was finished. dgge profiles of periodical sludge samples showed that dominant microbial s ... | 2009 | 19402426 |
| carotenoids of gemmatimonas aurantiaca (gemmatimonadetes): identification of a novel carotenoid, deoxyoscillol 2-rhamnoside, and proposed biosynthetic pathway of oscillol 2,2'-dirhamnoside. | gemmatimonas aurantiaca strain t-27(t) is an orange-coloured, gram-negative, facultatively aerobic, polyphosphate-accumulating bacterium belonging to a recently proposed phylum, gemmatimonadetes. we purified its pigments and identified them as carotenoids and their glycoside derivatives using spectral data. the major carotenoid was (2s,2' s)-oscillol 2,2'-di-(alpha-l-rhamnoside), and the minor carotenoids were (2s)-deoxyoscillol 2-( alpha-l-rhamnoside) and didemethylspirilloxanthin. deoxyoscillo ... | 2010 | 19959572 |
| bacterial diversity, pigments and nitrogen fixation of biological desert crusts from the sultanate of oman. | biological desert crusts are relatively common in the arid deserts of the sultanate of oman; however, little is known about their microbial community composition and role in soil fertilization. we compared three crusts from geographically different locations for their soil texture, bacterial community structure, pigment composition and nitrogenase activity. the crusts were growing on alkaline (ph 7.6-8.7) loamy sand and silty loam soils. microscopically, microcoleus vaginatus was the most abunda ... | 2010 | 20298501 |
| petroleum-influenced beach sediments of the campeche bank, mexico: diversity and bacterial community structure assessment. | the bacterial diversity and community structure were surveyed in intertidal petroleum-influenced sediments of ~100 km of a beach, in the southern gulf of mexico. the beach was divided in twenty sampling sites according to high, moderate and low petroleum influence. densities of cultured heterotrophic (hab) and hydrocarbon degrading bacteria (hdb) were highly variable in sediments, with little morphological assortment in colonies. pcr-risa banding patterns differentiated distinct communities alon ... | 2011 | 21802196 |
| molecular evolution of bacterial indoleamine 2,3-dioxygenase. | indoleamine 2,3-dioxygenase (ido) and tryptophan 2,3-dioxygenase (tdo) are tryptophan-degrading enzymes that catalyze the first step in l-trp catabolism via the kynurenine pathway. in mammals, tdo is mainly expressed in the liver and primarily supplies nicotinamide adenine dinucleotide (nad(+)). tdo is widely distributed from mammals to bacteria. active ido enzymes have been reported only in vertebrates and fungi. in mammals, ido activity plays a significant role in the immune system while in fu ... | 2011 | 21689736 |