| employment of 16 s rdna gene sequencing techniques to identify culturable environmental eubacteria in a tertiary referral hospital. | universal or 'broad-range' eubacterial polymerase chain reaction (pcr) was performed on 53 isolates from environmental water-associated sites in a haematology unit (n = 22) and the outer surfaces of cleaning lotion containers sited throughout a tertiary referral hospital (n = 31) 16 s rdna pcr was performed using two sets of universal primers, including the novel reverse primer, xb4, to generate a composite amplicon of 1068 bp, which was sequenced to obtain each isolate's identity. sequence anal ... | 2004 | 15142716 |
| stability of microbial communities in goat milk during a lactation year: molecular approaches. | the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ... | 2007 | 17604934 |
| brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth promoting potential. | a gram-positive, aerobic, non-motile, coccoid shaped, halotolerant bacterium (jg 06 t) was isolated from roots of salicornia brachiata, an extreme halophyte. phylogenetic analysis based on 16s rrna gene sequence showed sequence similarities of 99.2% to brachybacterium paraconglomeratum, 99.0% to b. conglomeratum, and 98.2% to b. faecium. dna-dna hybridization with the close relatives brachybacterium paraconglomeratum (dsm 46341t), b. conglomeratum (dsm 10241 t), b. faecium (dsm 4810t), b. tyrofe ... | 2011 | 21216918 |
| brachybacterium aquaticum sp. nov., a novel actinobacterium isolated from seawater. | a pale-yellowish bacterium, strain kws-1t, was isolated from seawater during a study of the bacterial diversity of the marine environment of the kanyakumari coastal region of the bay of bengal, india, and was studied by using a polyphasic taxonomic approach. strain kws-1t had morphological and chemotaxonomic properties (cell-wall diamino acid, menaquinone and fatty acid profile) consistent with its classification in the genus brachybacterium. phylogenetic analysis of the 16s rrna gene sequence s ... | 2016 | 27514821 |