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dietary supplementation of bacillus subtilis and fructooligosaccharide enhance the growth, non-specific immunity of juvenile ovate pompano, trachinotus ovatus and its disease resistance against vibrio vulnificus.a feeding trial was conducted to investigate the effects of dietary administration of probiotic bacillus subtilis and prebiotic fructooligosaccharide (fos) on growth performance, immune responses and disease resistance of juvenile ovate pompano, trachinotus ovatus. one thousand six hundred and twenty individuals (initial body weight: 10.32 ± 0.46 g, mean ± s.e) were fed nine practical diets according to a 3 × 3 factorial design: the basal diet as the control diet supplemented with three levels o ...201424614017
studies on the isolation of photobacterium damselae subsp. piscicida from diseased golden pompano (trachinotus ovatus linnaeus) and antibacterial agents sensitivity.an epizootic occurred among cultured golden pompano (trachinotus ovatus); it involved mass mortality on fish farms in linshui in hainan province, china, in 2008. diseased fish exhibited no obvious clinical signs, but pathological studies showed that nodules were scattered over the spleen and kidney. to investigate the nature of the pathogen, we studied the surviving fish, and a gram-negative bacterium (designated strain tos1) was isolated from the spleens of golden pompano. pathogenicity assays ...201323140939
establishment and characterization of a cell line from the head kidney of golden pompano trachinotus ovatus and its application in toxicology and virus susceptibility.a cell line derived from the head kidney of golden pompano trachinotus ovatus (tohk) was established and characterized in this study. the tohk cells grew most rapidly at 28° c and the optimum foetal bovine serum concentration in l-15 medium was 10%. the tohk cells have a diploid chromosome number of 2n = 54. the transfection efficiency of tohk cells was 7·5% at the 15th passage and 72% at the 40th passage. the transfection efficiency in tohk cells was high, so these cells are suitable for foreig ...201728271507
establishment and characterization of a mid-kidney cell line derived from golden pompano trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies.golden pompano trachinotus ovatus, a popularly cultured and commercially important marine fish worldwide, has been recognized as a promising candidate for mariculture. however, outbreaks of infectious bacterial or viral diseases and environmental deterioration have led to great economic losses in t. ovatus aquaculture recently. in our research, we established a new mid-kidney cell line, designated as tok, from golden pompano, t. ovatus. the optimized growth temperature and working concentration ...201627981423
establishment and characterization of a novel cell line from the brain of golden pompano (trachinotus ovatus).golden pompano is a commercially important marine fish that is widely cultured in china, japan, and southeast asian countries but has been seriously threatened by pathogen. a novel cell line (togb) derived from the brain of golden pompano trachinotus ovatus was established and characterized in this study. togb cell line showed high virus susceptibility, especially grouper nervous necrosis virus (gnnv) and singapore grouper iridovirus (sgiv). as one of the most devastating viruses in marine fish ...201626822430
[fatty acid composition of edible marine fish in zhoushan, zhejiang province].to analyze the main fatty acids in edible marine fish from zhoushan, zhejiang province.201324113107
establishment of a new cell line from the snout tissue of golden pompano trachinotus ovatus, and its application in virus susceptibility.a new marine-fish cell line, designated gps, was established from the snout tissue of golden pompano trachinotus ovatus. gps cells multiplied well in leibovitz's l-15 containing 10% foetal bovine serum (fbs) at 28° c and the cells have been subcultured for >60 passages. polymerase chain reaction (pcr) amplification of 16s ribosomal (r)rna confirmed the origin of this cell line from t. ovatus. chromosome analysis showed that gps cells exhibited chromosomal aneuploidy with a modal chromosome numbe ...201627146361
massive consumption of gelatinous plankton by mediterranean apex predators.stable isotopes of carbon and nitrogen were used to test the hypothesis that stomach content analysis has systematically overlooked the consumption of gelatinous zooplankton by pelagic mesopredators and apex predators. the results strongly supported a major role of gelatinous plankton in the diet of bluefin tuna (thunnus thynnus), little tunny (euthynnus alletteratus), spearfish (tetrapturus belone) and swordfish (xiphias gladius). loggerhead sea turtles (caretta caretta) in the oceanic stage an ...201222470416
effect of dietary carbohydrate on non-specific immune response, hepatic antioxidative abilities and disease resistance of juvenile golden pompano (trachinotus ovatus).the present study was conducted to investigate the effects of dietary carbohydrate (cho) levels on non-specific immune responses, hepatic antioxidative status and disease resistance of juvenile golden pompano. fish were fed six isonitrogenous and isoenergetic diets containing various cho levels for 8 weeks. after the feeding trial, fish were challenged by vibrio harveyi and survival rate was recorded for the next 12 days. plasma total protein and albumin content, respiratory burst activity, alka ...201425181652
the effects of dietary soybean isoflavones on growth, innate immune responses, hepatic antioxidant abilities and disease resistance of juvenile golden pompano trachinotus ovatus.the present study was conducted to investigate the effects of dietary soybean isoflavones (si) supplementation on growth performance, innate immune responses, hepatic antioxidant abilities, heat shock protein 70 (hsp70) gene expression and resistance to the pathogen vibrio harveyi in trachinotus ovatus. a basal diet was supplemented with si at 0, 10, 20, 40, 60, 80 mg kg(-1) feed for 8 weeks. significantly maximum weight gain (wg) and specific growth rate (sgr) were observed in treatment with 40 ...201525541076
epidemiological characterization of vnnv in hatchery-reared and wild marine fish on hainan island, china, and experimental infection of golden pompano (trachinotus ovatus) juveniles.the current epidemiological situation of viral nervous necrosis virus (vnnv) on hainan island was investigated. a total of 490 hatchery-reared fish and 652 wild fish were sampled for vnnv detection from march 2013 to may 2014. positive detection rates of 84.53% (153/181) and 0.97 % (3/309) were obtained in diseased and healthy hatchery-reared samples, respectively, by conventional rt-pcr. however, using more-sensitive nested rt-pcr, the positive detection rates in healthy hatchery-reared fish re ...201526350771
molecular characterization and functional analysis of a peroxiredoxin 1 cdna from golden pompano (trachinotus ovatus).peroxiredoxin 1 (prx 1) is an important antioxidant protein that can protect organisms against the toxicity of reactive oxygen species. in this study, a full-length prx 1 cdna sequence (toprx 1) was identified from golden pompano (trachinotus ovatus). the toprx 1 cdna was 1049 base pairs (bp) long and contained a 5'-untranslated region (utr) of 127 nucleotides, a 3'-utr of 328 nucleotides, and a 594 bp open reading frame (orf) encoding a 197 amino acid polypeptide. the toprx 1 protein showed str ...201525889122
draft genome sequence of nocardia seriolae zj0503, a fish pathogen isolated from trachinotus ovatus in china.nocardia seriolae is a pathogen that causes nocardiosis in marine and freshwater fish. here, we report the draft genome sequence of n. seriolae strain zj0503, which was isolated from trachinotus ovatus in guangdong, china.201525555736
[structural changes in mitochondrion-rich cells in the gills of artificial selected trachinotus ovatus offspring under different salinities].distribution and ultrastructural changes in the mitochondrion-rich cells in gills of artificial selected trachinotus ovatus under different salinities (5, 20, and 30) were examined by light and transmission electron micrograph. results indicated that the mitochondrion-rich cells were mainly present on the base of the gill filaments and branchial leaflets, and the volume and quantity of mitochondrion-rich cells increased with salinity. all three salinity groups had apical crypts, which were const ...201323913893
detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification.red-spotted grouper nervous necrosis virus (rgnnv) causes high mortality in marine fish larvae cultured in china. to control better an outbreak of this virus, a rapid, specific and sensitive detection method based on loop-mediated isothermal amplification (lamp) was developed. a set of four primers, two outer and two inner, was designed from rgnnv genome rna. the lamp reaction mix was optimized. the method was specific as no cross-reaction was observed between white spot syndrome virus, koi herp ...201019755132
characterization and identification of streptococci from golden pompano in china.streptococcal infections cause significant mortality and high economic losses in the fish farm industry worldwide, including in the culture of golden pompano trachinotus ovatus l., a species gaining popularity in china. a total of 9 streptococcal strains were isolated from cage-cultured diseased golden pompano in beihai, zhanjing, and shenzhen, china, between 2012 and 2014. conventional and rapid identification systems were used to determine that the isolates were streptococcus agalactiae, s. in ...201627225204
construction of a streptococcus agalactiae phob mutant and evaluation of its potential as an attenuated modified live vaccine in golden pompano, trachinotus ovatus.streptococcus agalactiae is a gram-positive pathogen that can survive inside professional phagocytes and nonphagocytic cells to cause septicemia and meningoencephalitis in freshwater and marine fish. however, vaccines based on extracellular products (ecp) and formalin-killed whole s. agalactiae cells, as well as subunit vaccine are unable to protect fish from infection by variant serotypes s. agalactiae. the search for live attenuated vaccine with highly conserved and virulent-related genes is e ...201727884809
morphology and molecular analysis of life-cycle stages of proctoeces maculatus (looss, 1901) (digenea: fellodistomidae) in the bizerte lagoon, tunisia.the life cycle of proctoeces maculatus (looss, 1901) (digenea, fellodistomidae) was studied in bizerte lagoon (tunisia). three sequential hosts appear to be involved: the mediterranean mussel mytilus galloprovincialis lamarck, 1819 (mytilidae) as the first intermediate host; the polychaete sabella pavonina savigny, 1822 (sabellidae), as the second intermediate host; and fishes (lithognathus mormyrus (linnaeus, 1758) (sparidae), trachinotus ovatus (linnaeus, 1758) (carangidae) and sparus aurata l ...201626694018
thioredoxin of golden pompano involved in the immune response to photobacterium damselae.thioredoxin (trx) is one of the key systems responsible for keeping the intracellular environment in a highly reduced state. in this study, a full-length trx cdna sequence (totrx) from golden pompano trachinotus ovatus was identified after pyrosequencing of golden pompano cdna library. totrx cdna is comprised of 786 bp, and contained a 324 bp open reading frame (orf) encoding a 107 amino acid polypeptide, a 5' untranslated region (utr) of 116 bp, and a long 3'- utr of 346 bp. multiple sequence a ...201526052015
cloning and expression analysis of c-type lysozyme gene in golden pompano, trachinotus ovatus.it is well known that lysozymes are key proteins to teleosts in the innate immune system and possess high bactericidal properties. in the present study, a c-type lysozyme gene (to-lysc) was cloned from golden pompano, trachinotus ovatus. the to-lysc cdna is composed of 743 bp with a 36 bp of 5'-utr, 432 bp open reading frame (orf) and 275 bp 3'-utr, encoding a polypeptide of 144 amino acids (genbank accession no: kt935522). phylogenetic analysis revealed that to-lysc showed highest similarity to ...201627150051
dietary administration of chitooligosaccharides to enhance growth, innate immune response and disease resistance of trachinotus ovatus.the present study was conducted to investigate the effects of dietary chitooligosaccharides (cos) supplementation on the innate immune response and protection against vibrio harveyi infection in trachinotus ovatus. a basal diet was supplemented with 0.0 (control), 2.0, 4.0 and 6.0 g cos kg(-1) to formulate four experimental diets. each diet was randomly allocated to triplicate groups of fish in floating sea cages (1.5 × 1.0 × 2.0 m), and each cage was stocked with 80 fish (initial average weight 10 ...201222366310
dietary inclusion of salmon, herring and pompano as oily fish reduces cvd risk markers in dyslipidaemic middle-aged and elderly chinese women.dietary intervention studies to assess the cardioprotective effects of oily fish are scarce in china. the present study aimed to examine the effects of the oily fish, norwegian salmon, herring and local farmed pompano (trachinotus ovatus) on cvd risk markers when included in the chinese diet. in this 8-week, parallel-arm, randomised intervention study, 126 chinese women with hypertriacylglycerolaemia, aged 35-70 years, were assigned to four groups to consume an experimental lunch containing 80 g ...201222221492
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