| identification and characterization of a novel β-galactosidase from victivallis vadensis atcc baa-548, an anaerobic fecal bacterium. | victivallis vadensis atcc baa-548 is a gram-negative, anaerobic bacterium that was isolated from a human fecal sample. from the genomic sequence of v. vadensis, one gene was found to encode agarase; however, its enzymatic properties have never been characterized. the gene encoding the putative agarase (ncbi reference number zp_01923925) was cloned by pcr and expressed in e. coli rosetta-gami by using the inducible t(7) promoter of pet28a(+). the expressed protein with a 6×his tag at the n-termin ... | 2012 | 23274992 |
| genome sequence of victivallis vadensis atcc baa-548, an anaerobic bacterium from the phylum lentisphaerae, isolated from the human gastrointestinal tract. | victivallis vadensis atcc baa-548 represents the first cultured representative from the novel phylum lentisphaerae, a deep-branching bacterial lineage. few cultured bacteria from this phylum are known, and v. vadensis therefore represents an important organism for evolutionary studies. v. vadensis is a strictly anaerobic sugar-fermenting isolate from the human gastrointestinal tract. | 2011 | 21398537 |
| victivallis vadensis gen. nov., sp. nov., a sugar-fermenting anaerobe from human faeces. | a novel strictly anaerobic, cellobiose-degrading bacterium, strain cellot, was isolated from a human faecal sample by combining enrichments in liquid and soft-agar basal media. a noteworthy characteristic was its inability to grow on normal agar plates and in roll tubes. the cells were coccus shaped and non-motile, with an extracellular slime layer. growth of strain cellot occurred between 20 and 40degrees c, with optimal growth at 37 degrees c. the ph range for growth was 5-7.5 with an optimum ... | 2003 | 12656175 |
| detection of wwe2-related lentisphaerae by 16s rrna gene sequencing and fluorescence in situ hybridization in landfill leachate. | we collected samples of anaerobic landfill leachate from municipal solid waste landfill (vert-le-grand, france) and constructed 16s rrna clone libraries using primers targeting planctomycetes and relatives (pla46f and 1390r). analyses of 16s rrna gene sequences resulted in the abundant representation of wwe2-related lentisphaerae, members of the phylum lentisphaerae, in the clone library (98% of the retrieved sequences). although the sequences that are phylogenetically affiliated with the cultur ... | 2010 | 20962908 |
| performance evaluation and bacteria analysis of afb-mfc enriched with high-strength synthetic wastewater. | in order to study the performance and bacterial communities of an anaerobic fluidized bed microbial fuel cell (afb-mfc) system, the 16s rdna gene sequencing was applied, and high-strength synthetic wastewater was treated by the afb-mfc system. the high-strength synthetic wastewater, in which the concentrations of chemical oxygen demand (cod), nitrite nitrogen, and nitrate nitrogen were above 19,000, 2,516-3,871 and 927-1,427 mg/l, was treated by the afb-mfc system. the removal efficiency of cod, ... | 2014 | 24434962 |