| antibody response and virus tissue distribution in chickens inoculated with wild-type and recombinant fowl adenoviruses. | we demonstrated that the long tandemly repeated region (tr-2) is dispensable for in vitro replication of fowl adenovirus 9 (fadv-9). the tr-2-deleted recombinant fadv-9 expressing the enhanced green fluorescence protein was further characterized for in vivo effects. groups of chickens were exposed to recombinant or wild-type fadv-9 by intramuscular injection, through the feed or drinking water and one group served as a negative control. the antibody (ab) response, evaluated by elisa and a plaque ... | 2003 | 14604569 |
| a broadly applicable method to characterize large dna viruses and adenoviruses based on the dna polymerase gene. | many viral pathogens are poorly characterized, are difficult to culture or reagents are lacking for confirmatory diagnoses. we have developed and tested a robust assay for detecting and characterizing large dna viruses and adenoviruses. the assay is based on the use of degenerate pcr to target a gene common to these viruses, the dna polymerase, and sequencing the products. | 2006 | 16608526 |
| the non-essential left end region of the fowl adenovirus 9 genome is suitable for foreign gene insertion/replacement. | the goals of this study were to demonstrate that a non-essential region at the left end of the fowl adenovirus 9 (fadv-9) genome could be used to generate recombinant viruses, examine their in vitro growth characteristics and determine their ability to transduce non-avian cells. three fadv-9 vectors (rfadv-9s) were generated carrying the enhanced-green fluorescent protein (egfp) gene: fadv-9inegfp, fadv-9 delta 1-egfp and fadv-9 delta 4-egfp. fadv-9inegfp carried the egfp cassette inserted into ... | 2010 | 20132849 |
| fowl adenovirus-based vaccine platform. | nonpathogenic fowl adenoviruses (fadvs) are amenable for engineering multivalent vaccine platforms due to large stretches of nonessential dna sequences in their genomes. we describe the generation of fadv-9-based vaccine platforms by targeted homologous recombination in an infectious clone (ppacfadv-9 or wild type fadmid) containing the entire viral genome in a cosmid vector. the viral dna is subsequently released from the cosmid by restriction enzyme digestion followed by transfection in a chic ... | 2017 | 28374242 |
| characterization and functional studies of fowl adenovirus 9 dutpase. | deoxyuridine 5'-triphosphate pyrophosphatase (dutpase), a ubiquitous enzyme that catalyzes the hydrolysis of dutp to dump and found in many viruses, has yet to be identified in fowl adenovirus 9 (fadv-9). by a multiple alignment of dutpase amino acid sequences, fadv-9 orf1 contained the five conserved motifs that define the protein family, and encoded a functional dutpase. moreover, transcription and protein expression patterns were characterized, indicating that dutpase was transcribed from 2h ... | 2016 | 27498408 |
| oral inoculation of chickens with a candidate fowl adenovirus 9 vector. | fowl adenoviruses (fadvs) are a potential alternative to human adenovirus-based vaccine vectors. our previous studies demonstrated that a 2.4-kb region at the left end of the fadv-9 genome is nonessential for virus replication and is suitable for the insertion or replacement of transgenes. our in vivo study showed that the virus fadv-9δ4, lacking six open reading frames (orfs) at the left end of its genome, replicates less efficiently than wild-type fadv-9 (wtfadv-9) in chickens that were infect ... | 2013 | 23740924 |
| foreign gene expression and induction of antibody response by recombinant fowl adenovirus-9-based vectors with exogenous promoters. | fowl adenoviruses (fadvs) are promising vectors for poultry vaccines and gene therapy. the commonly used human cytomegalovirus (cmv) promoter in recombinant fadv-9 viruses (recfadv-9s) leads to foreign gene expression that elicits an antibody response. despite its strength, studies have shown that the cmv promoter is prone to silencing by methylation hampering the in vivo application of vectors containing this promoter. therefore, to improve our virus vector system and circumvent potential limit ... | 2017 | 28780115 |
| rapid generation of fowl adenovirus 9 vectors. | fowl adenoviruses (fadv) have the largest genomes of any fully sequenced adenovirus genome, and are widely considered as excellent platforms for vaccine development and gene therapy. as such, there is a strong need for stream-lined protocols/strategies for the generation of recombinant adenovirus genomes. current genome engineering strategies rely upon plasmid based homologous recombination in escherichia coli bj5183. this process is time-consuming, involves multiple cloning steps, and low effic ... | 2015 | 26238923 |
| Antibody response and virus shedding of chickens inoculated with left end deleted fowl adenovirus 9-based recombinant viruses. | The nonpathogenic fowl adenoviruses (FAdVs) are suitable recombinant virus vectors. Two different replication-competent FAdV-9-based recombinant viruses carrying the enhanced green fluorescent protein (EGFP) gene within a nonessential DNA sequence at the left end genomic region were tested in chickens to study the antibody response by enzyme-linked immunosorbent assay to both the foreign proteins, EGFP and FAdV-9, and virus shedding through the feces. All inoculations were done intramuscularly: ... | 2011 | 22017044 |
| a region at the left end of the fowl adenovirus 9 genome that is non-essential in vitro has consequences in vivo. | the regions at the left and right ends of fowl adenovirus (fadv) genomes are not well-characterized in comparison to those of human adenoviruses. using a series of deletion mutants, we analysed a 2.4 kb region near the left end of the fadv-9 genome (nt 400-2782) that contains packaging-signal motifs vi and vii and open reading frames (orfs) 0, 1, 1a, 1b, 1c and 2. viable viruses with specific deletions in this region had wild-type characteristics in vitro, as measured by cytopathic effect, plaqu ... | 2010 | 19759237 |
| unique features of fowl adenovirus 9 gene transcription. | we examined the transcriptional organization of fowl adenovirus 9 (fadv-9) and analyzed temporal transcription profiles of its early and late mrnas. at least six early and six late transcriptional regions were identified for fadv-9. extensive splicing was observed in all fadv-9 early transcripts examined. sequence analysis of the cdnas representing the early proteins identified untranslated leader sequences, precise locations of splice donor and acceptor sites, as well as polyadenylation signals ... | 2002 | 12441071 |
| the long repeat region is dispensable for fowl adenovirus replication in vitro. | two regions containing tandemly repeated sequences are present in the fowl adenovirus 9 (fadv-9) genome. the longer repeat region (tr-2) is composed of 13 contiguous 135-bp-long direct repeats, the function of which is unknown. an infectious fadv-9 genomic clone, constructed by homologous recombination in escherichia coli, was used for engineering of recombinant viruses. the enhanced green fluorescence protein (egfp) coding sequence was cloned in both rightward and leftward orientations so as to ... | 2001 | 11336545 |
| fowl aviadenovirus 9 dutpase plays a role in regulation of the host immune response. | fowl aviadenoviruses (fadvs) are distributed worldwide in poultry farms. some fadvs are the causative agents of inclusion body hepatitis and hydropericardium syndrome that cause significant economic losses to the poultry industry. in contrast with human adenovirus, the study of the molecular biology of fadv is still far behind. we previously showed that fadv-9 open reading frame 1 (orf1) is a dutpase enzyme that contributes to the upregulation of type i interferons and is not required for virus ... | 2017 | 28945170 |
| fowl adenovirus 9 orf19, a lipase homolog, is nonessential for virus replication and is suitable for foreign gene expression. | fowl adenovirus 9 (fadv-9) has one of the largest genomes (45 kb) so far sequenced from all adenoviruses studied. genus-specific genes located within the early (e) regions at the right and left ends of the viral genome have unknown functions except for orf8 (gam-1 gene), orf22 and orf1 (dutpase gene). orf19, located at the right end of the genome (nts 34,220-36,443), is predicted to encode a lipase protein and its homologs are also found in all fadv genomes so far sequenced. the role of orf19 in ... | 2019 | 30529235 |