cloning, characterization and expression of a bifunctional fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from potato.we have isolated cdna clones encoding the regulatory enzyme fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase from a potato (solanum tuberosum) leaf cdna library. all clones represented transcripts of the same gene (f2kp1). functionality of the encoded protein was verified by expression of the active enzyme in escherichia coli. the expressed enzyme had both kinase activity which forms fructose-2,6-bisphosphate from fructose-6-phosphate and atp, and phosphatase activity which degrade fruc ...199910350085
metabolic engineering of high carotenoid potato tubers containing enhanced levels of beta-carotene and order to enhance the carotenoid content of potato tubers, transgenic potato plants have been produced expressing an erwinia uredovora crtb gene encoding phytoene synthase, specifically in the tuber of solanum tuberosum l. cultivar desiree which normally produces tubers containing c. 5.6 microg carotenoid g(-1) dw and also in solanum phureja l. cv. mayan gold which has a tuber carotenoid content of typically 20 microg carotenoid g(-1) dw. in developing tubers of transgenic crtb desiree lines, ...200515533882
agrobacterium-mediated transformation of solanum phureja.a population of transgenic plants was produced by the transformation of internodal explants of solanum phureja, db337/37 (the cultivar mayan gold) using an agrobacterium tumefaciens lba4404-based vector containing a phytoene synthase gene (crtb). the regeneration strategy utilised a two-step protocol, with a 12-day callus induction stage mediated by 1.07 microm alpha-napthaleneacetic acid (naa), 7.10 microm zeatin riboside and 0.06 microm gibberellic acid (ga3), followed by a prolonged (up to 90 ...200515666166
transcriptome responses to ralstonia solanacearum infection in the roots of the wild potato solanum commersonii.solanum commersonii is a wild potato species that exhibits high tolerance to both biotic and abiotic stresses and has been used as a source of genes for introgression into cultivated potato. among the interesting features of s. commersonii is resistance to the bacterial wilt caused by ralstonia solanacearum, one of the most devastating bacterial diseases of crops.201525880642
complete genome sequence of a novel potato virus s strain infecting solanum phureja in colombia.potato virus s (pvs) (genus carlavirus, family betaflexiviridae) is one of the most prevalent viruses in potato crops (solanum tuberosum and s. phureja) around the world, causing reductions in crop yields between 10 and 20 %. symptoms of pvs infection may include leaf mottling, rugosity of leaves, deepening of the veins and reductions in crop yields between 10 and 20 %. virions are flexuous rods of 610-710 nm with a positive-sense ssrna genome of approximately 8500 nt comprising six orfs, a 5'ca ...201323665769
plant defense genes associated with quantitative resistance to potato late blight in solanum phureja x dihaploid s. tuberosum hybrids.markers corresponding to 27 plant defense genes were tested for linkage disequilibrium with quantitative resistance to late blight in a diploid potato population that had been used for mapping quantitative trait loci (qtls) for late blight resistance. markers were detected by using (i) hybridization probes for plant defense genes, (ii) primer pairs amplifying conserved domains of resistance (r) genes, (iii) primers for defense genes and genes encoding transcriptional regulatory factors, and (iv) ...200212059107
differential gene expression in two potato lines differing in their resistance to phytophthora infestans.horizontal resistance to late blight in the potato is a primary objective of many breeding programs. knowledge of the physiological and biochemical mechanisms underlying it, however, is scarce. the purpose of the present study was the identification of these physiological and biochemical factors in plant material obtained by crossing a late blight resistant solanum phureja clone with a susceptible dihaploid of s. tuberosum subsp. tuberosum. the mrna rt-pcr differential display method was used to ...200312872493
qtl analysis of late blight resistance in a diploid potato family of solanum phureja x s. stenotomum.field resistance to phytophthora infestans (mont.) de bary, the causal agent of late blight in potatoes, has been characterized in a potato segregating family of 230 full-sib progenies derived from a cross between two hybrid solanum phureja x s. stenotomum clones. the distribution of area under the disease progress curve values, measured in different years and locations, was consistent with the inheritance of multigenic resistance. relatively high levels of resistance and transgressive segregati ...200515931506
the rpi-blb2 gene from solanum bulbocastanum is an mi-1 gene homolog conferring broad-spectrum late blight resistance in potato.the necessity to develop potato and tomato crops that possess durable resistance against the oomycete pathogen phytophthora infestans is increasing as more virulent, crop-specialized and pesticide resistant strains of the pathogen are rapidly emerging. here, we describe the positional cloning of the solanum bulbocastanum-derived rpi-blb2 gene, which even when present in a potato background confers broad-spectrum late blight resistance. the rpi-blb2 locus was initially mapped in several tetraploi ...200516212601
the novel, major locus rpi-phu1 for late blight resistance maps to potato chromosome ix and is not correlated with long vegetation period.despite the long history of breeding potatoes resistant to phytophthora infestans, this oomycete is still economically the most important pathogen of potato worldwide. the correlation of high levels of resistance to late blight with a long vegetation period is one of the bottlenecks for progress in breeding resistant cultivars of various maturity types. solanum phureja was identified as a source of effective late blight resistance, which was transferred to the cultivated gene pool by interspecif ...200616835764
detection of a quantitative trait locus for both foliage and tuber resistance to late blight [phytophthora infestans (mont.) de bary] on chromosome 4 of a dihaploid potato clone (solanum tuberosum subsp. tuberosum).linkage analysis, kruskal-wallis analysis, interval mapping and graphical genotyping were performed on a potato diploid backcross family comprising 120 clones segregating for resistance to late blight. a hybrid between the solanum tuberosum dihaploid clone pdh247 and the long-day-adapted s. phureja clone db226(70) had been crossed to db226(70) to produce the backcross family. eighteen aflp primer combinations provided 186 and 123 informative maternal and paternal markers respectively, with 63 ma ...200616845519
marker-assisted selection of diploid and tetraploid potatoes carrying rpi-phu1, a major gene for resistance to phytophthora infestans.the rpi-phu1 gene originates from an interspecific hybrid between solanum stenotomum and s. phureja, and confers a high level of resistance to phytophthora infestans (late blight) in potato. the rpi-phu1 was introduced by crossing at the diploid level into the s. tuberosum gene pool and then transferred to the tetraploid level by means of 2n gametes. tetraploid lines carrying the rpi-phu1 were selected for further crosses. a molecular marker gp94, linked in mapping population 97-30 with the rpi- ...201020453300
resistance gene enrichment sequencing (renseq) enables reannotation of the nb-lrr gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations.renseq is a nb-lrr (nucleotide binding-site leucine-rich repeat) gene-targeted, resistance gene enrichment and sequencing method that enables discovery and annotation of pathogen resistance gene family members in plant genome sequences. we successfully applied renseq to the sequenced potato solanum tuberosum clone dm, and increased the number of identified nb-lrrs from 438 to 755. the majority of these identified r gene loci reside in poorly or previously unannotated regions of the genome. seque ...201323937694
a locus conferring effective late blight resistance in potato cultivar sárpo mira maps to chromosome xi.late blight of potato, caused by phytophthora infestans, is one of the most economically important diseases worldwide, resulting in substantial yield losses when not adequately controlled by fungicides. late blight was a contributory factor in the great irish famine, and breeding for resistance to the disease began soon after. several disease-resistant cultivars have subsequently been obtained, and amongst them sárpo mira is currently one of the most effective. the aim of this work was to extend ...201424343200
de novo pyrimidine biosynthesis in the oomycete plant pathogen phytophthora infestans.the oomycete phytophthora infestans, causal agent of the tomato and potato late blight, generates important economic and environmental losses worldwide. as current control strategies are becoming less effective, there is a need for studies on oomycete metabolism to help identify promising and more effective targets for chemical control. the pyrimidine pathways are attractive metabolic targets to combat tumors, virus and parasitic diseases but have not yet been studied in phytophthora. pyrimidine ...201424361203
genome sequence of a divergent colombian isolate of potato virus v (pvv) infecting solanum phureja.deep sequencing analysis of the transcriptome of a solanum phureja cv. criolla colombia plant with symptoms typical of a virus disease revealed an infection with potato virus v (pvv). the pvv-phureja genome comprises 9904 nt, exhibits 83% nucleotide identity with currently fully sequenced pvv isolates and contains one large orf that codes for a polyprotein of 3065 residues flanked by 5' and 3' utr of 217 and 448 nt, respectively. phylogenetic analysis of the pvv-phureja polyprotein indicates tha ...201626982467
transfer of resistance to plrv titer buildup from solanum etuberosum to a tuber-bearing solanum gene pool.hybrids between solanum etuberosum and s. pinnatisectum harboring resistance to titer buildup of potato leafroll virus (plrv) were reciprocally crossed with tuber-bearing wild species s. acaule and s. verrucosum. a total of 47 hybrids with acaule were obtained with the aid of embryo rescue and sterile culturing of embryos from imbibing seeds. all but two hybrids with acaule had low pollen stainabilities or were pollen sterile. hybrid seeds from crosses with verrucosum were easily obtained, and t ...198824231994
comparison of compatible and incompatible response of potato to meloidogyne susceptible (d6) and two resistant (e2 and n4) clones of solanum sparsipilum x (s. phureja x haploid of s. tuberosum) were used to study the responses of potato roots and tubers to race 1 of meloidogyne incognita (kofoid &white) chitwood. the compatible response was characterized by rapid penetration of large numbers of second-stage juveniles (j2) into roots, cessation of root growth, and occasional curving of root tips. the life cycle of m. incognita in the susceptible clone was completed i ...198719290132
evolution of nematode-resistant mi-1 gene homologs in three species of solanum.plants have evolved several defense mechanisms, including resistance genes. resistance to the root-knot nematode meloidogyne incognita has been found in wild plant species. the molecular basis for this resistance has been best studied in the wild tomato solanum peruvianum and it is based on a single dominant gene, mi-1.2, which is found in a cluster of seven genes. this nematode attacks fiercely several crops, including potatoes. the genomic arrangement, number of copies, function and evolution ...201121207065
a resistance gene against potato late blight originating from solanum × michoacanum maps to potato chromosome vii.solanum ×  michoacanum (bitter.) rydb. is a diploid, 1 ebn (endosperm balance number) nothospecies, a relative of potato originating from the area of morelia in michoacán state of mexico that is believed to be a natural hybrid of s. bulbocastanum × s. pinnatisectum. both parental species and s. michoacanum have been described as sources of resistance to phytophthora infestans (mont.) de bary. the gene for resistance to potato late blight, rpi-mch1, originating from s. michoacanum was mapped to t ...201121987281
comparative sequence analysis of the potato cyst nematode resistance locus h1 reveals a major lack of co-linearity between three haplotypes in potato (solanum tuberosum ssp.).the h1 locus confers resistance to the potato cyst nematode globodera rostochiensis pathotypes 1 and 4. it is positioned at the distal end of chromosome v of the diploid solanum tuberosum genotype sh83-92-488 (sh) on an introgression segment derived from s. tuberosum ssp. andigena. markers from a high-resolution genetic map of the h1 locus (bakker et al. in theor appl genet 109:146-152, 2004) were used to screen a bac library to construct a physical map covering a 341-kb region of the resistant ...201021049265
[analysis of microsatellite locus polymorphism in potato (solanum tuberosum) cultivars of russian breeding].polymorphism of microsatellite loci of the nuclear genome was examined in 29 cultivars and accessions of wild potato (s. tuberosum, s. stoloniferum, s. demissum, and s. phureja). nine ssp markers, most informative (pic = 0.61-0.92) for genotyping of the cultivars of russian breeding were selected. polymorphism of the selected ssr loci was characterized, and prevailing, as well as unique ssr allele phenotypes were described. a total of 87 allele phenotypes were identified. the highest number of a ...201020536018
rpi-vnt1.1, a tm-2(2) homolog from solanum venturii, confers resistance to potato late blight.despite the efforts of breeders and the extensive use of fungicide control measures, late blight still remains a major threat to potato cultivation worldwide. the introduction of genetic resistance into cultivated potato is considered a valuable method to achieve durable resistance to late blight. here, we report the identification and cloning of rpi-vnt1.1, a previously uncharacterized late-blight resistance gene from solanum venturii. the gene was identified by a classical genetic and physical ...200919348576
characterization of phytophthora infestans populations in colombia: first report of the a2 mating type.phytophthora infestans, the causal agent of late blight in crops of the solanaceae family, is one of the most important plant pathogens in colombia. not only are solanum lycopersicum, and s. tuberosum at risk, but also several other solanaceous hosts (physalis peruviana, s. betaceum, s. phureja, and s. quitoense) that have recently gained importance as new crops in colombia may be at risk. because little is known about the population structure of phytophthora infestans in colombia, we report her ...200919055438
phylogenetic relationships of phytophthora andina, a new species from the highlands of ecuador that is closely related to the irish potato famine pathogen phytophthora infestans.phylogenetic relationships of phytophthora infestans sensu lato in the andean highlands of south america were examined. three clonal lineages (us-1, ec-1, ec-3) and one heterogeneous lineage (ec-2) were found in association with different host species in genus solanum. the ec-2 lineage includes two mitochondrial (mtdna) haplotypes, ia and ic. isolates of p. infestans sensu lato ec-2 fit the morphological description of p. infestans but are different from any genotypes of p. infestans described t ...200818833752
measurement of viroid sequence homology by hybridization with complementary dna prepared in vitro.dna complementary to potato spindle tuber viroid (ystv cdna) has been used in rna dna hybridization experiments to study pstv replication in a variety of hosts and to measure the amount of sequence homology between ystv and several independently isolated viroids. when pstv cdna was hybridized with low molecular weight rna isolated from pstv-infected tomato at different times after inoculation, pstv . pstv cdna hybrids were detectable before symptoms began to appear. low molecular weight rna was ...197818627881
origin and evolution of andigena potatoes revealed by chloroplast and nuclear dna markers.andigena potatoes (solanum tuberosum l. subsp. andigena hawkes) (2n = 4x = 48) are important, native-farmer-selected cultivars in the andes, which form a primary gene pool for improving a worldwide grown potato (s. tuberosum subsp. tuberosum). to elucidate the origin of andigena, 196 andigena accessions were compared with 301 accessions of 33 closely related cultivated and wild species using several types of chloroplast dna (ctdna) markers and nuclear dna (ndna) restriction fragment length polym ...200616936843
assessment of genetic variability of haploids extracted from tetraploid (2n = 4x = 48) solanum tuberosum.the objectives of this study were to assess the genetic variability of haploids (2n = 2x = 24) extracted from tetraploid solanum tuberosum through 4x x 2x crosses with solanum phureja. molecular and phenotypic analyses were performed to fingerprint the genotypes used and to evaluate their potential use in breeding programs. aflp analysis revealed the presence of specific bands derived from the tetraploid seed parent s. phureja, as well as ex novo originated bands. on average, 210 bands were visu ...200415284867
resistance to bacterial wilt in somatic hybrids between solanum tuberosum and solanum phureja.somatic hybrid plants were produced after protoplast electrofusion between a dihaploid potato, cv. bf15, and a wild tuber-bearing relative, solanum phureja, with a view to transferring bacterial wilt resistance into potato lines. a total of ten putative hybrids were selected. dna analysis using flow cytometry revealed that six were tetraploids, two mixoploids, one amphiploid and one octoploid. in the greenhouse, the putative hybrids exhibited strong vigor and were morphologically intermediate, i ...200011164589
structural diversity of the patatin gene family in potato cv. desiree.we have used a combined genetical and molecular approach to study the structural diversity of the patatin gene family in tetraploid solanum tuberosum l. cv. desiree (2n = 4x = 48). nine dihaploid derivatives (2n = 2x = 24) of cv. desiree were isolated by gynogenesis through prickle pollination with s. phureja juz et buk. patatin dna sequences in desiree and in the dihaploids were examined by probing southern blots of restriction endonucleases hindiii and xbai digested dna with patatin cdna regio ...19882841572
the pin family of proteins in potato and their putative role in tuberization.the pin family of trans-membrane proteins mediates auxin efflux throughout the plant and during various phases of plant development. in arabidopsis thaliana, the pin family comprised of 8 members, divided into "short" and "long" pins according to the length of the hydrophilic domain of the protein. based on sequence homology using the recently published potato genome sequence (solanum tuberosum group phureja) we identified ten annotated potato stpin genes. mining the publicly available gene expr ...201324391658
molecular and phylogenetic characterization of the sieve element occlusion gene family in fabaceae and non-fabaceae plants.the phloem of dicotyledonous plants contains specialized p-proteins (phloem proteins) that accumulate during sieve element differentiation and remain parietally associated with the cisternae of the endoplasmic reticulum in mature sieve elements. wounding causes p-protein filaments to accumulate at the sieve plates and block the translocation of photosynthate. specialized, spindle-shaped p-proteins known as forisomes that undergo reversible calcium-dependent conformational changes have evolved ex ...201020932300
transposon based activation tagging in diploid strawberry and monoploid derivatives of potato.diploid strawberry and potato transformed with a transposon tagging construct exhibited either global (strawberry) or local transposition (potato). an activation tagged, compact-sized strawberry mutant overexpressed the gene adjacent to ds. as major fruit and vegetable crops, respectively, strawberry and potato are among the first horticultural crops with draft genome sequences. to study gene function, we examined transposon-tagged mutant strategies in model populations for both species, fragari ...201424728112
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