| laboratory and field studies on glugea stephani (hagenmuller), a microsporidan parasite of pleuronectid flatfishes. | the microsporidan glugea stephani is a common parasite of juvenile english sole (parophrys vetulus) in yaquina bay, oregon. field observations indicated that fish became infected only in the upper estuary where summer temperatures were above 15c and the incidence of infection reached 79.8% in the late fall. laboratory infections developed and parasite growth occurred only at or above 15c. the parasite was successfully transmitted to juvenile english sole by brine shrimp (artemia salina) and amph ... | 1976 | 1271307 |
| overview of studies on liver carcinogenesis in english sole from puget sound; evidence for a xenobiotic chemical etiology. ii: biochemical studies. | the levels of aromatic hydrocarbons in sediments of puget sound, washington, are positively correlated with the prevalence of hepatic neoplasms and related lesions in english sole (parophrys vetulus). to investigate the biochemical processes involved in chemical carcinogenesis in fish from puget sound, we have studied the uptake, activation, and detoxication of polycyclic aromatic hydrocarbons (pahs) in english sole, and have compared these data to pah metabolism in a related species, starry flo ... | 1990 | 2360039 |
| formation and persistence of benzo[a]pyrene-diolepoxide-dna adducts in liver of english sole (parophrys vetulus). | the formation of dna adducts from the carcinogenic environmental pollutant benzo[a]pyrene (bap) was investigated in liver of english sole (parophrys vetulus), a fish species that exhibits a high prevalence of liver neoplasms in several polycyclic aromatic hydrocarbon (pah)-contaminated areas of puget sound, wa. analysis by the 32p-postlabeling assay of hepatic dna digests from english sole exposed parenterally to bap showed the presence of bap-diol epoxide (bapde)-dna adducts. when english sole ... | 1989 | 2495192 |
| chemical carcinogenesis in feral fish: uptake, activation, and detoxication of organic xenobiotics. | the high prevalence of liver neoplasms in english sole (parophrys vetulus) and substantially lower prevalence of neoplasms in a closely related species, starry flounder (platichthys stellatus) captured from industrialized waterways, provide a unique opportunity to compare biochemical processes involved in chemical carcinogenesis in feral fish species. because levels of aromatic hydrocarbons (ahs) in urban sediments are correlated with prevalences of liver neoplasms in english sole, we have initi ... | 1987 | 3297658 |
| comparative metabolism of benzo(a)pyrene and covalent binding to hepatic dna in english sole, starry flounder, and rat. | metabolism of benzo(a)pyrene (bap) in vivo and in vitro was studied using two benthic fish species, english sole (parophrys vetulus) and starry flounder (platichthys stellatus), and sprague-dawley rats. at 24 h after administration of bap (7.9 mumol/kg of body weight) to fish either p.o. (experiment 1) or i.p. (experiment 2), the specific activity of binding of bap metabolites to hepatic dna (pmol of bap equivalent per mg of dna) was higher in sole [2.1 in experiment 1; 28 +/- 5 (se) in experime ... | 1986 | 3731058 |
| hepatic cytochrome p-450 isozymes and aryl hydrocarbon hydroxylase in english sole (parophrys vetulus). | | 1986 | 3741486 |
| bacteriology of spoilage of fish muscle. iv. role of protein. | clarified muscle press juice from english sole (parophrys vetulus) was fractionated by gel filtration into a protein and a protein-free fraction. upon inoculation with spoilage bacteria, the protein fraction failed to show any signs of spoilage, but the protein-free fraction spoiled according to the usual organoleptic and chemical criteria. despite its spoilage-resistant qualities, the protein fraction accelerated spoilage of the protein-free fraction when the two were combined. protein breadkow ... | 1967 | 6049302 |
| characterization of benzo[a]pyrene metabolites isolated from muscle, liver, and bile of a juvenile flatfish. | juvenile english sole (parophrys vetulus) were force fed [3h]benzo[a]pyrene (bap) and, after 24 h, metabolites were isolated from bile, liver, and muscle. the metabolites were analyzed by t.l.c. and h.p.l.c., and further characterized by u.v., fluorescence, or m.s. analyses. the identities of bap 7,8-dihydrodiol, bap 9,10-dihydrodiol, 1-hydroxy bap, 3-hydroxy bap, and 9-hydroxy bap were confirmed. the amounts of bap 7,8-dihydrodiol plus its conjugates (sulfates and glucuronides) were greater tha ... | 1982 | 6295656 |
| effects of low temperature on the development of the microsporidan glugea stephani in english sole (parophrys vetulus). | glugea stephani requires temperatures above 15 c for development in juvenile pleuronectid flatfishes in yaquina bay, oregon. the effect of low temperature (10 c) on the development of recently established parasites was tested experimentally in juvenile english sole (parophrys vetulus). low temperature arrested parasite development, but did not kill the protozoan which resumed development on return to 19-20 c after as long as 42 days at 10 c. no parasites detectable with the light microscope were ... | 1981 | 6802989 |
| isolation, characterization, and radioimmunoassay of atlantic halibut somatolactin and plasma levels during stress and reproduction in flatfish. | somatolactin (sl), a recently identified teleost pituitary hormone which is a member of the growth hormone/prolactin family, was isolated from pituitary tissue of atlantic halibut (hippoglossus hippoglossus). pituitary proteins were extracted in ammonium bicarbonate (ph 7.8), fractionated using gel filtration chromatography, and purified using reversed-phase high-performance liquid chromatography. halibut sl was identified on the basis of molecular size (determined by gel electrophoresis and mas ... | 1997 | 9038252 |
| complete cdna sequence of the ki-ras proto-oncogene in the liver of wild english sole (pleuronectes vetulus) and mutation analysis of hepatic neoplasms and other toxicopathic liver lesions. | a complete copy of ki-ras b cdna from english sole (pleuronectes vetulus), a benthic marine flatfish, was cloned and sequenced. the percent identity between the predicted amino acid sequence of english sole and human ki-ras b was 97%, whereas the percent identity between the english sole gene and rainbow trout or rivulus ki-ras b was 98%. areas of amino-acid sequence conservation included codons 12, 13, and 61, the positions in which mutations are observed in ras cellular oncogenes in other spec ... | 1998 | 9869449 |
| an enzyme-linked immunosorbent assay (elisa) for measuring vitellogenin in english sole (pleuronectes vetulus): development, validation and cross-reactivity with other pleuronectids. | vitellogenin (vtg) is a yolk protein produced in the liver of oviparous animals in response to estrogen. vitellogenesis is normally observed only in sexually mature females, but it can be induced in male and juvenile animals by exposure to exogenous estradiol (e2) or substances that mimic estrogens. the abnormal production of vtg by males can, therefore, be used as a biological indicator for exposure to xenoestrogens. in this study, an enzyme-linked immunosorbent assay (elisa) for measuring vtg ... | 1998 | 9972314 |
| structure, expression and activation of fish ras genes. | ras genes encode proteins that play a central role in cell growth signaling cascades. the fish ras genes characterized to date, have a high degree of nucleotide sequence and deduced amino acid similarity with the mammalian ras gene counterparts. a large proportion and wide variety of mammalian tumors possess mutant forms of ras. in such cases, the localization of ras mutations has been restricted to exons i and ii, and to codons 12, 13 and 61. experimental exposure of fish to a range of genotoxi ... | 2001 | 11551618 |
| isolation and characterization of the retinoblastoma protein from fish. | the retinoblastoma (rb) gene represents the first tumor suppressor gene characterized. the encoded protein, prb, plays a crucial role in cell cycle control, preventing malignant cell proliferation. recently, homologues of the rb gene have been isolated in fish and the pocket domain, which is central to rb function, was conserved. in our studies, using coelocanth (latimeria chalumnae), rainbow trout (oncorhynchus mykiss), medaka (oryzias latipes) and english sole (parophrys vetulus), we have deve ... | 2001 | 11567901 |
| bacteriology of spoilage of fish muscle. ii. incidence of spoilers during spoilage. | a test medium consisting of a sterile raw press juice from fish muscle was used to determine the incidence of spoilage bacteria on stored fillets of english sole (parophrys vetulus). the initial load of spoilers was shown to be consistently below 10%. this percentage rose but slightly toward the middle of the spoilage runs, and actually declined when spoilage was most apparent both organoleptically and chemically. further evidence implicating the pseudomonas and achromobacter groups in the spoil ... | 1964 | 14170967 |
| bacteriology of spoilage of fish muscle. 3. characterization of spoilers. | a total of 807 bacterial isolates from fresh and spoiling fillets of english sole (parophrys vetulus) stored at 5 c were classified as to genus and tested for various biochemical activities, including the ability to spoil sterile muscle press juice at 5 c. production of off-odor, volatile reducing substances, and trimethylamine was used to estimate spoilage. it was found that (i) spoilers could be distinguished from nonspoilers on the basis of the juice spoilage test, (ii) differentiation betwee ... | 1965 | 14339271 |
| temporal changes in the prevalence of parasites in two oregon estuary-dwelling fishes. | the parasite faunas of juvenile english sole (parophrys vetulus) in 1971-1972 and staghorn sculpin (leptocottus armatus) in 1971 from yaquina bay, oregon, were compared with faunas found in the same estuary in 1997-2000 (english sole) and 1999-2000 (staghorn sculpin). the 7 most commonly occurring parasites in 1971 were compared with the same species observed during the same month and sampling sites in 1997-2000. multivariate community analysis of juvenile english sole parasites supported the su ... | 2004 | 15270100 |
| hemoflagellates of oregon marine fishes with the description of new species of trypanosoma and trypanoplasma. | of 2,122 marine fishes representing 36 species collected in the northeastern pacific ocean in the vicinity of newport, oregon from 1971 to 1973, 541 individuals (25.5%) representing 8 species (22.2%) were infected with hemoflagellates. four morphologically distinct trypanosomes and 3 distinct trypanoplasms were found in fishes collected offshore, but no hemoflagellates were observed in fishes from yaquina bay estuary. trypanosoma pacifica was found in english sole parophrys vetulus, pacific sand ... | 2007 | 18314692 |
| toxic chemicals in marine sediment and biota from mukilteo, washington: relationships with hepatic neoplasms and other hepatic lesions in english sole (parophrys vetulus). | high prevalences of idiopathic liver lesions, including 7.5% hepatic neoplasms (e.g., hepatocellular and cholangiocellular carcinomas) and 16.7% foci of cellular alteration (putative preneoplastic hepatic lesions), were found in english sole (parophrys vetulus) from waters near mukilteo, a small community on puget sound in washington state. sediments from the sampling sites contained particularly high concentrations of aromatic hydrocarbons. substantially lower concentrations of these compounds ... | 2013 | 3856055 |
| pathology of hepatomas and other liver abnormalities in english sole (parophrys vetulus) from the duwamish river estuary, seattle, washington. | liver abnormalities were found, by gross and histopathologic examination, in 92% of the english sole (parophrys vetulus) from the duwamish river estuary, seattle, washington. hepatomas were found in 32% (20 of 62) of the english sole. other observed liver aberrations included increased fatty vacuolation, congestion, structure disarray, increased size and number of melanin-macrophage centers, centrolobular fatty degeneration and necrosis, increased amounts of perivascular connective tissue, inter ... | 1993 | 206710 |
| detection of the intranuclear microsporidium nucleospora salmonis in naturally and experimentally exposed chinook salmon oncorhynchus tshawytscha by in situ hybridization. | nucleospora salmonis, an intranuclear microsporidian parasite of salmonid fish, is often difficult to observe in histological sections or wet mount preparations from lightly infected tissues because of its small size and location within the nuclei of lymphoblast-type cells. diagnosis of infections by conventional light microscopy is directly dependent upon distinguishing different stages of the parasite from host cell nuclear material or vacuoles. to assist detection of stages of the parasite in ... | 2007 | 17605006 |
| molecular detection and phylogenetic placement of a microsporidian from english sole (pleuronectes vetulus) affected by x-cell pseudotumors. | flatfish tissue samples exhibiting x-cell pseudotumors were tested with a number of ribosomal dna (rdna) general primers in polymerase chain reactions (pcrs). microsporidian primers resulted in the amplification of an rdna fragment and molecular phylogenetic analysis indicated that although the organism did not relate closely with any current microsporidian genera, it was most similar to nucleospora salmonis and branched within the enterocytozoonidae. re-examination of the original tissues used ... | 2000 | 10958476 |
| conservation of a glutathione s-transferase in marine and freshwater fish. | we have previously reported the isolation and cloning of glutathione s-transferase (gst) cdnas from two marine fish, english sole (pleuronectes vetulus) and starry flounder (platichthys stellatus), that exhibited > 95% identity to plaice (pleuronectes platessa) gst-a aquatic toxicol., 44, 171-182]. in the present study, we have used reverse transcription-polymerase chain reaction (rt-pcr) analysis to isolate a 471 nucleotide gst-like cdna from largemouth bass (micropterus salmoides) liver. seque ... | 2003 | 11460686 |
| individual-based energetic model suggests bottom up mechanisms for the impact of coastal hypoxia on pacific harbor seal (phoca vitulina richardii) foraging behavior. | wind-driven coastal hypoxia represents an environmental stressor that has the potential to drive redistribution of gilled marine organisms, and thereby indirectly affect the foraging characteristics of air-breathing upper trophic-level predators. we used a conceptual individual-based model to simulate effects of coastal hypoxia on the spatial foraging behavior and efficiency of a marine mammal, the pacific harbor seal (phoca vitulina richardii) on the oregon coast. habitat compression of fish wa ... | 2017 | 28082128 |
| two new species of lepeophtheirus (copepoda: caligidae) parasitic on fishes from southern california waters. | two new species of lepeophtheirus are described and illustrated based on a collection from the orange county sewer outfall, california. they are: l. remiopsis sp.nov. from the branchial cavities and external body surfaces of parophrys vetulus girard, hippoglossina stomata eigenmann & eigenmann, pleuronichthys verticalis (jordan & gilbert), chitonotus pugetensis (steindachner), and porichthys notatus (girard); and l. rotundipes sp.nov. from the external body surfaces and occasionally the gill cav ... | 1979 | 481904 |
| trypanosoma pacifica sp. n. from the english sole parophrys vetulus girard from oregon. | | 1972 | 4641901 |