| poly i:c inhibits the expression of channel catfish virus immediate-early gene orf 1 at early times after infection. | channel catfish virus (ccv) is a herpesvirus that infects channel catfish fry and fingerlings. previous research has demonstrated that type i interferons inhibit the expression of immediate-early (ie) genes of some mammalian herpesviruses. however, ccv is distantly related to the mammalian herpesviruses and type i interferons from higher vertebrates exhibit only 20% similarity to fish interferons. in this work we demonstrate that treatment of channel catfish ovary (cco) cells, a fibroblast-like ... | 2007 | 17303437 |
| genome sequences of three koi herpesvirus isolates representing the expanding distribution of an emerging disease threatening koi and common carp worldwide. | since the mid-1990s, lethal infections of koi herpesvirus (khv) have been spreading, threatening the worldwide production of common carp and koi (both cyprinus carpio). the complete genome sequences of three khv strains from japan, the united states, and israel revealed a 295-kbp genome containing a 22-kbp terminal direct repeat. the finding that 15 khv genes have clear homologs in the distantly related channel catfish virus (ictalurid herpesvirus 1) confirms the proposed place of khv in the fam ... | 2007 | 17329333 |
| differential susceptibility of blue catfish, ictalurus furcatus (valenciennes), channel catfish, i. punctatus (rafinesque), and blue x channel catfish hybrids to channel catfish virus. | | 2008 | 18086038 |
| an overlapping bacterial artificial chromosome system that generates vectorless progeny for channel catfish herpesvirus. | herpesviruses are important pathogens of humans and other animals. herpesvirus infectious clones that can reconstitute phenotypically wild-type (wt) virus are extremely valuable tools for elucidating the roles of specific genes in virus pathophysiology as well as for making vaccines. ictalurid herpesvirus 1 (channel catfish herpesvirus [ccv]) is economically very important and is the best characterized of the herpesviruses that occur primarily in bony fish and amphibians. here, we describe the c ... | 2008 | 18234790 |
| molecular confirmation of a new herpesvirus from catfish (ameiurus melas) by testing the performance of a novel pcr method, designed to target the dna polymerase gene of alloherpesviruses. | a pcr method with consensus degenerate primers was developed for the detection of herpesviruses (hvs) of anamnia. compared to previously published pcrs, targeting the dna polymerase gene of fish hvs, the size of pcr products was more than tripled. although broad applicability of the method could not be proven, approximately 1,600-bp fragments from hvs of white sturgeon (acipenser transmontanus) and black bullhead (ameiurus melas) were obtained and sequenced. phylogenetic tree reconstructions sho ... | 2008 | 18972184 |
| the order herpesvirales. | the taxonomy of herpesviruses has been updated by the international committee on taxonomy of viruses (ictv). the former family herpesviridae has been split into three families, which have been incorporated into the new order herpesvirales. the revised family herpesviridae retains the mammal, bird and reptile viruses, the new family alloherpesviridae incorporates the fish and frog viruses, and the new family malacoherpesviridae contains a bivalve virus. three new genera have been created in the f ... | 2009 | 19066710 |
| experimental annotation of channel catfish virus by probabilistic proteogenomic mapping. | experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. channel catfish herpesvirus (ccv) is a simple comparative model for understanding herpesvirus biology and the evolution of the herpesviridae. the canonical ccv genome has 76 predicted orf and only 12 of these have been confirmed experimentally. we describe a modifica ... | 2009 | 19391180 |
| validation of a qpcr assay for the detection of ictalurid herpesvirus-2 (ichv-2) in fish tissues and cell culture supernatants. | ictalurid herpesvirus-2 (ichv-2) is a pathogen of cultured black bullhead, ameiurus melas (rafinesque), and has been shown to produce high mortality in experimental exposures of channel catfish, ictalurus punctatus (rafinesque). during acute infections, the virus grows readily in cell cultures but produces a cytopathic effect (cpe) similar to that of ictalurid herpesvirus-1 (ichv-1) and the channel catfish reovirus. we have developed a quantitative pcr assay that can be used to detect ichv-2 in ... | 2010 | 20082661 |
| partial genome analysis of siberian sturgeon alloherpesvirus suggests its close relation to acihv-2 - short communication. | partial genome sequence of a herpes-like virus, isolated from siberian sturgeon (acipenser baeri), was determined and subjected to phylogenetic analysis. the virus (sbshv) has been shown to be the causative agent of an acute disease with high mortality in farmed juvenile sturgeons in russia. two fragments (of 7000 and 300 base pairs in length) encompassing 3 complete and 3 partial orfs were amplified by pcr. sturgeon herpesvirus strains, classified into species acipenserid herpesvirus 2 (acihv-2 ... | 2010 | 20460226 |
| in vitro evaluation of marine-microorganism extracts for anti-viral activity. | viral-induced infectious diseases represent a major health threat and their control remains an unachieved goal, due in part to the limited availability of effective anti-viral drugs and measures. the use of natural products in drug manufacturing is an ancient and well-established practice. marine organisms are known producers of pharmacological and anti-viral agents. in this study, a total of 20 extracts from marine microorganisms were evaluated for their antiviral activity. these extracts were ... | 2010 | 20691099 |
| comparative analysis of a conserved gene block from the genome of the members of the genus ictalurivirus. | objective: partial genome sequences were determined and subjected to comparative analyses from two fish herpesviruses (hvs). acipenserid (aci) hv-2, originating from the white sturgeon (acipenser transmontanus), and ictalurid (ic) hv-2, isolated from the black bullhead (ameiurus melas), are recently approved species of the genus ictalurivirus of the family alloherpesviridae. methods: an almost 8,000-base-pair fragment, spanning between the genes of the dna polymerase and the atpase subunit of th ... | 2011 | 21228534 |
| Partial genome characterization of acipenserid herpesvirus 2: taxonomical proposal for the demarcation of three subfamilies in Alloherpesviridae. | Sequencing of approximately one half of the genome of acipenserid herpesvirus 2 (AciHV-2), which is a member of the genus Ictalurivirus in the family Alloherpesviridae, revealed that the gene organization is very similar to that of ictalurid herpesvirus 1 (IcHV-1), the founder member of the genus. The sequenced region encodes the AciHV-2 homologues of IcHV-1 ORF24 to ORF69. It contains 46 predicted protein-coding regions, including 12 that seem to have a homologue in every alloherpesvirus genome ... | 2011 | 21935624 |
| Antibody response of channel catfish after channel catfish virus infection and following dexamethasone treatment. | Channel catfish virus (CCV, Ictalurid herpesvirus 1) and CCV disease have been extensively studied. Yet, little is known about CCV-host interaction after resolution of the primary infection. In order to determine potential recrudescence of CCV from latency, we established latency by exposing channel catfish juveniles with CCV or a thymidine kinase-negative recombinant (CCVlacZ) at a dose that caused less than 20% mortality. Then, we evaluated antibody response by serially sampling the same fish ... | 2011 | 21932530 |