| human parechoviruses--biology and clinical significance. | a new genus of the family picornaviridae, parechovirus, has recently been recognised on the basis of distinctive biological and molecular properties. in particular: parechoviruses exhibit characteristic effects on the host cell; cleavage of the capsid protein vp0, required for maturation of the virus particle in most other picornaviruses, does not occur; there is a unique extension, which is highly basic in character, to the n-terminus of the capsid protein vp3; and the 2a protein, in common wit ... | 2000 | 10654005 |
| detection of all known parechoviruses by real-time pcr. | the parechovirus genus of the picornaviridae family contains two species, human parechovirus (hpev) and ljungan virus (lv). the hpevs (including the former echoviruses 22 and 23, now hpev type 1 (hpev1) and hpev2, respectively) cause a wide spectrum of disease, including aseptic meningitis, gastroenteritis, encephalitis, acute respiratory illness, and neonatal sepsis-like disease. the lvs were isolated from bank voles in sweden during a search for an infectious agent linked to fatal myocarditis ... | 2008 | 18524969 |
| characterization of polyclonal antibodies against the capsid proteins of ljungan virus. | ljungan virus (lv) is a suspected human pathogen isolated from voles in sweden and north america. to enable virus detection and studies of localization and activity of virion proteins, polyclonal antibodies were produced against bacterially expressed capsid proteins of the lv strain, 87-012g. specific detection of proteins corresponding to viral antigens in lysates of lv infected cells was demonstrated by immunoblotting using each one of the generated polyclonal antibodies. in addition, native v ... | 2008 | 18403027 |
| a highly divergent picornavirus in a marine mammal. | nucleic acids from an unidentified virus from ringed seals (phoca hispida) were amplified using sequence-independent pcr, subcloned, and then sequenced. the full genome of a novel rna virus was derived, identifying the first sequence-confirmed picornavirus in a marine mammal. the phylogenetic position of the tentatively named seal picornavirus 1 (sepv-1) as an outlier to the grouping of parechoviruses was found consistently in alignable regions of the genome. a mean protein sequence identity of ... | 2008 | 17942560 |
| characterization of beta-cell function of pancreatic islets isolated from bank voles developing glucose intolerance/diabetes: an animal model showing features of both type 1 and type 2 diabetes mellitus, and a possible role of the ljungan virus. | bank voles (clethrionomys glareolus) kept in captivity develop diabetes mellitus to a significant extent. also in wild bank voles, elevated blood glucose has been observed. a newly isolated picornavirus named ljungan virus (lv) has been found in the pancreas of these bank voles. moreover, lv infection in combination with environmental factors may cause glucose intolerance/diabetes (gint/d) in normal mice. the aim of the present study was to investigate the functional characteristics of pancreati ... | 2007 | 17686482 |
| replication of ljungan virus in cell culture: the genomic 5'-end, infectious cdna clones and host cell response to viral infections. | ljungan virus (lv) is a picornavirus recently isolated from bank voles (clethrionomys glareolus). the previously uncharacterised 5'-end sequence of the lv genome was determined. infectious cdna clones were constructed of the wild type lv prototype strain 87-012 and of the cytolytically replicating cell culture adapted variant 87-012g. virus generated from cdna clones showed identical growth characteristics as uncloned virus stocks. cell culture adapted lv, 87-012g, showed a clear cytopathic effe ... | 2007 | 17645978 |
| human parechovirus 1 infections in young children--no association with type 1 diabetes. | the epidemiology, transmission and clinical symptoms of human parechoviruses [hpev, classified earlier as enteroviruses; echovirus 22 (hpev1) and echovirus 23 (hpev2)] remain poorly characterized. enteroviruses and one parechovirus species, the ljungan virus, have been associated with type 1 diabetes in humans and rodents. the occurrence of human parechovirus 1 (hpev1) infections in young children and their possible association with type 1 diabetes was evaluated. the prospective birth cohort stu ... | 2007 | 17311340 |
| a new quantitative real-time reverse transcriptase pcr assay and melting curve analysis for detection and genotyping of ljungan virus strains. | ljungan virus (lv), a new member of the picornaviridae, recently isolated from vole species in both sweden and the usa, is suspected to be pathogenic for humans as an aetiological agent in myocarditis, diabetes, neurological disease and perinatal disease. this study describes for the first time an rt-pcr assay that can identify and quantify lv infection in various tissue sample types using primers and two different minor-groove-binder probes targeting the 5'-untranslated region of the lv genome. ... | 2007 | 17196265 |
| intrauterine death, fetal malformation, and delayed pregnancy in ljungan virus-infected mice. | a picornavirus (ljunganvirus [lv]) has recently been associated with disease during pregnancy in its natural rodent reservoir and in humans. a study of laboratory mice infected under controlled conditions was therefore undertaken. | 2006 | 16894624 |
| diabetes and myocarditis in voles and lemmings at cyclic peak densities--induced by ljungan virus? | although it is well-documented from theoretical studies that pathogens have the capacity to generate cycles, the occurrence and role of pathogens and disease have been poorly empirically studied in cyclic voles and lemmings. in screening for the occurrence of disease in cyclic vole and lemming populations, we found that a high proportion of live-trapped clethrionomys glareolus, c. rufocanus, microtus agrestis and lemmus lemmus at high collective peak density, shortly before the decline, suffered ... | 2006 | 16868760 |
| no ljungan virus rna in stool samples from the norwegian environmental triggers of type 1 diabetes (midia) cohort study. | ljungan virus (ljv) has been proposed as a potential environmental factor for type 1 diabetes. the objective was to test for any association of ljv with type 1 diabetes. | 2010 | 20185738 |
| prenatal viral exposure followed by adult stress produces glucose intolerance in a mouse model. | it has been suggested that the uterine environment may influence metabolic disease occurring later in adult life, and that adult stress may promote disease outcome. using a mouse model, we tested whether in utero exposure to ljungan virus (lv) followed by adult exposure to stress produces diabetes. the influence of the timing of viral exposure over the course of pregnancy was also tested. | 2006 | 16821045 |
| phylogeny of the picornaviridae and differential evolutionary divergence of picornavirus proteins. | a rooted phylogenetic analysis of the viral family picornaviridae identified several major clusters within this viral family. the genus parechovirus, including ljungan virus, was found to be basal to the family. within the family, there were two major subgroups: (1) a cluster including viruses assigned to the genera enterovirus and rhinovirus; and (2) a cluster including teschovirus, cardiovirus, erbovirus, aphthovirus, and probably kobuvirus. within the former cluster, the genera enterovirus an ... | 2004 | 15157632 |
| cell culture propagation and biochemical analysis of the ljungan virus prototype strain. | ljungan virus (lv) is proposed as a potentially important rodent harbored viral human pathogen. little is known about the biophysical nature of the virus and despite being molecularly characterized, progress in epidemiological and basic biological studies of lv has been hampered by the lack of a robust and reliable cell culture propagation system. here we report the first description of an efficient lytic multi-cycle cell culture propagation of the lv prototype strain (87-012). biophysical analy ... | 2004 | 15094371 |
| type 1 diabetes in swedish bank voles (clethrionomys glareolus): signs of disease in both colonized and wild cyclic populations at peak density. | colonized bank voles (clethrionomys glareolus) originating from sweden developed type 1 diabetes. animals became polydipsic, glucosuric, and hyperglycemic and gradually developed a lethal ketoacidosis. pancreas in animals with end-stage disease showed total destruction of islet cells. interestingly, also a high proportion of wild bank voles in cyclic populations that were trapped at (or close to) the cyclic population density peak frequently showed high blood glucose levels and pathological gluc ... | 2003 | 14679053 |
| a model system for in vitro studies of bank vole borne viruses. | the bank vole (myodes glareolus) is a common small mammal in europe and a natural host for several important emerging zoonotic viruses, e.g. puumala hantavirus (puuv) that causes hemorrhagic fever with renal syndrome (hfrs). hantaviruses are known to interfere with several signaling pathways in infected human cells, and hfrs is considered an immune-mediated disease. there is no in vitro-model available for infectious experiments in bank vole cells, nor tools for analyses of bank vole immune acti ... | 2011 | 22194969 |
| rodents and risk in the mekong delta of vietnam: seroprevalence of selected zoonotic viruses in rodents and humans. | in the mekong delta in southern vietnam, rats are commonly traded in wet markets and sold live for food consumption. we investigated seroprevalence to selected groups of rodent-borne viruses among human populations with high levels of animal exposure and among co-located rodent populations. the indirect fluorescence antibody test (ifat) was used to determine seropositivity to representative reference strains of hantaviruses (dobrava virus [dobv], seoul virus [seov]), cowpox virus, arenaviruses ( ... | 0 | 25629782 |
| development of type 1 diabetes in wild bank voles associated with islet autoantibodies and the novel ljungan virus. | wild bank voles (clethrionomys glareolus) may develop diabetes in laboratory captivity. the aim of this study was to test whether bank voles develop type 1 diabetes in association with ljungan virus. two groups of bank voles were analyzed for diabetes, pancreas histology, autoantibodies to glutamic acid decarboxylase (gad65), ia-2, and insulin by standardized radioligand-binding assays as well as antibodies to in vitro transcribed and translated ljungan virus antigens. group a represented 101 tr ... | 2003 | 12745669 |
| molecular characterization of m1146, an american isolate of ljungan virus (lv) reveals the presence of a new lv genotype. | ljungan virus (lv) is a suspected human pathogen recently isolated from bank voles in sweden. this study describes the genetic characterization of a virus, m1146, which was isolated in 1962 from another vole species (microtus montanus), trapped in oregon, usa. based on antigenic properties, m1146 was postulated previously as a putative member of the family picornaviridae: the near complete genomic sequence verifies that m1146 is a member of the picornaviridae, most closely related to lvs isolate ... | 2003 | 12655084 |
| molecular analysis of three ljungan virus isolates reveals a new, close-to-root lineage of the picornaviridae with a cluster of two unrelated 2a proteins. | ljungan virus (lv) is a suspected human pathogen recently isolated from bank voles (clethrionomys glareolus). in the present study, it is revealed through comparative sequence analysis that three newly determined swedish lv genomes are closely related and possess a deviant picornavirus-like organization: 5' untranslated region-vp0-vp3-vp1-2a1-2a2-2b-2c-3a-3b-3c-3d-3' untranslated region. the lv genomes and the polyproteins encoded by them exhibit several exceptional features, such as the absence ... | 2002 | 12163611 |
| phylogenetic analysis of ljungan virus and a-2 plaque virus, new members of the picornaviridae. | in addition to the viruses belonging to the nine proposed genera of the picornaviridae, enterovirus, rhinovirus, cardiovirus, aphtovirus, hepatovirus, parechovirus, kobuvirus, erbovirus and teschovirus, two new members of this family have recently been discovered. three strains of ljungan virus (lv) were isolated from bank voles (clethrionomys glareolus) and a-2 plaque virus (a-2) was isolated from human sera. to study the genetic relationship between these recently discovered viruses and the me ... | 2002 | 11955639 |
| parechoviruses, a novel group of human picornaviruses. | parechoviruses are a recently established group of human viral pathogens. at the time of their first isolation, parechoviruses were classified among the enterovirus genus in the picornavirus family, but based on their different biological properties they were separated into their own genus. the type member is human parechovirus 1 (hpev1), which frequently infects humans, in particular small children. the parechovirus genus also includes hpev2 and the ljungan virus, which was recently isolated fr ... | 2001 | 11680794 |
| a new picornavirus isolated from bank voles (clethrionomys glareolus). | a previously unknown picornavirus was isolated from bank voles (clethrionomys glareolus). electron microscopy images and sequence data of the prototype isolate, named ljungan virus, showed that it is a picornavirus. the amino acid sequences of predicted ljungan virus capsid proteins vp2 and vp3 were closely related to the human pathogen echovirus 22 (approximately 70% similarity). a partial 5' noncoding region sequence of ljungan virus showed the highest degree of relatedness to cardioviruses. t ... | 1999 | 10049824 |
| zoonotic ljungan virus associated with central nervous system malformations in terminated pregnancy. | the ljungan virus (lv) has been shown to cause central nervous system malformations in laboratory mouse models. the lv has also been associated with intrauterine fetal death in humans. we investigated the presence of lv in a series of human hydrocephaly and anencephaly cases from elective abortions. | 2009 | 19180651 |
| ljungan virus present in intrauterine fetal death diagnosed by both immunohistochemistry and pcr. | following up on prior evidence from animal and human studies of ljungan virus (lv) in intrauterine fetal death (iufd), we examine additional cases of iufd using two standard assays of viral detection: immunohistochemistry (ihc) and real time rt-pcr. | 2009 | 19133649 |
| fetal death persists through recurrent pregnancies in mice following ljungan virus infection. | laboratory mice infected with ljungan virus (lv) early in pregnancy suffer from perinatal death. here we investigate the persistence of that effect through the outcome of consecutive pregnancies in lv-infected mice. | 2008 | 18850589 |
| longitudinal observation of parechovirus in stool samples from norwegian infants. | parechoviruses are assumed to be common infectious agents, but their epidemiologic and pathogenic properties are not well known. the aim of the present study was to assess the prevalence and molecular epidemiology of parechovirus in norwegian infants, as well as to investigate whether the presence of virus correlated with symptoms of infection. a group of 102 infants was longitudinally followed: 51 infants with a high genetic risk for type 1 diabetes (aged 3-35 months), and 51 children without t ... | 2008 | 18712841 |
| molecular characterization of a novel ljungan virus (parechovirus; picornaviridae) reveals a fourth genotype and indicates ancestral recombination. | ljungan virus (lv) was discovered 20 years ago in swedish bank voles (myodes glareolus, previously referred to as clethrionomys glareolus) during the search for an infectious agent causing lethal myocarditis in young athletes. to date, the genomes of four lv isolates, including the prototype 87-012 strain, have been characterized. three of these lv strains were isolated from bank voles trapped in sweden. sequence analysis of an american virus (m1146), isolated from a montane vole (microtus monta ... | 2009 | 19264646 |
| sudden infant death syndrome and ljungan virus. | ljungan virus (lv) has recently been associated with perinatal death in its natural rodent reservoir and also with developmental disorders of reproduction in laboratory mice. a strong epidemiological association has been found between small rodent abundance in sweden and the incidence of intrauterine fetal death (iufd) in humans. lv antigen has been detected in half of the iufd cases tested. the question was therefore raised whether sudden infant death syndrome (sids) might be associated with ro ... | 2009 | 19408134 |
| physicochemical properties of the ljungan virus prototype virion in different environments: inactivated by heat but resistant to acidic ph, detergents and non-physiological environments such as virkon-containing solutions. | it is of great importance to know how a virus particle is affected by environmental conditions. physicochemical properties of the virion will affect the virus viability in different environments, viral transmission between hosts, and will also be important for safe handling of the virus. the physicochemical properties of the ljungan virus (lv) prototype, 87-012, adapted to grow in cell culture were evaluated using both lv in crude cell extracts and purified virions. replication of lv was complet ... | 2007 | 17895601 |
| [irrelevant about ljungan virus]. | | 2009 | 19492680 |
| identification of amino acid residues of ljungan virus vp0 and vp1 associated with cytolytic replication in cultured cells. | ljungan virus is a picornavirus isolated from swedish and north american rodents. replication of ljungan virus in cultured cells normally induces a weak and delayed cytopathic effect compared to that of many other picornaviruses. however, efficiently replicating ljungan virus variants may evolve during serial passages in cell culture. in this study, we evaluate the significance of three substitutions in capsid protein vp0 and vp1 of a cell-culture-adapted variant of the swedish ljungan virus 145 ... | 2009 | 19585077 |
| the biobreeding rat diabetes model is infected with ljungan virus. | | 2007 | 17406852 |
| association of zoonotic ljungan virus with intrauterine fetal deaths. | it has recently been shown that ljungan virus (lv) is associated with disease in its wild rodent reservoir. in addition, it has been demonstrated that lv causes malformations and perinatal death in a mouse model. the question was therefore raised whether lv is a zoonotic agent in humans. | 2007 | 17335057 |
| a commentary on the possible association of ljungan virus and sids and issues in sids research. | | 2009 | 19757217 |
| antiviral treatments reduce severity of diabetes in ljungan virus-infected cd-1 mice and delay onset in diabetes-prone bb rats. | the effects of lv in two different species, cd-1 mice, without a genetic disposition for diabetes, and bb rats prone to t1d were examined. male cd-1 mice that had been exposed to lv in utero developed a type 2-like diabetes with increased blood glucose, insulin levels and epididymal fat at the age of 10-15 weeks. combination therapy including lv-antiserum and an antiviral drug, pleconaril, significantly reduced the levels of blood glucose and insulin and the amount of abdominal fat. in bb rats, ... | 2009 | 19780970 |
| ljungan virus detected in bank voles (myodes glareolus) and yellow-necked mice (apodemus flavicollis) from northern italy. | identified in 1998, ljungan virus (lv; picornaviridae) causes type 1 diabetes-like symptoms and myocarditis in bank voles (myodes glareolus) from sweden and denmark, and may be a zoonotic agent of several important diseases (e.g., intrauterine fetal death, type 1 diabetes, guillain-barré syndrome, and myocarditis). using a real-time reverse transcriptase-polymerase chain reaction assay and sequence analysis, we detected lv in bank voles, and for the first time, in yellow-necked mice (apodemus fl ... | 2010 | 20090041 |
| parechovirus typing in clinical specimens by nested or semi-nested pcr coupled with sequencing. | the parechovirus genus (picornaviridae) contains two known species, human parechovirus (hpev) and ljungan virus (lv). hpevs cause a wide spectrum of disease, including meningitis, gastroenteritis, encephalitis, respiratory illness, and neonatal sepsis-like disease. lvs are associated with diabetes and myocarditis in bank voles and have been proposed to cause disease in humans. the ability to rapidly and accurately type parechoviruses is critical to understanding their role in human disease. | 2010 | 20472496 |
| development and characterization of murine monoclonal antibodies to first and second ljungan virus genotypes. | ljungan virus (lv) is a rodent pathogen that causes diabetes and myocarditis in its natural host. in addition, lv has been associated with human disease during pregnancy and of neonates, respectively. a panel of 22 monoclonal antibodies (mabs) against first and second lv genotypes were produced by immunization of balb/c mice with whole virus. thirteen mabs were class igg antibodies and nine were class igm antibodies; all of them contained kappa light chains. all mabs were reactive with lv by cap ... | 2012 | 22579938 |
| ljungan virus: a commentary on its association with fetal and infant morbidity and mortality in animals and humans. | epidemiologic and experimental data support the notion that ljungan virus (lv), endemic in some rodent populations in sweden, denmark, and the united states, can cause morbidity and mortality in animals and humans. lv infection can cause type i diabetes mellitus, myocarditis, and encephalitis in bank voles and experimental mice, and lemmings. mouse dams infected with lv experience high rates of stillbirth that may persist across generations, and their fetuses may develop cranial, brain, and limb ... | 2010 | 20890937 |
| diabetes in danish bank voles (m. glareolus): survivorship, influence on weight, and evaluation of polydipsia as a screening tool for hyperglycaemia. | previous studies have concluded that the development of polydipsia (pd, a daily water intake ôëñ21 ml) among captive danish bank voles, is associated with the development of a type 1 diabetes (t1d), based on findings of hyperglycaemia, glucosuria, ketonuria/-emia, lipemia, destroyed beta cells, and presence of autoantibodies against gad65, ia-2, and insulin. | 2011 | 21829666 |
| current views on ljungan virus and its relationship to human diabetes. | | 2011 | 21739462 |
| serological evaluation of possible exposure to ljungan virus and related parechovirus in autoimmune (type 1) diabetes in children. | exposure to ljungan virus (lv) is implicated in the risk of autoimmune (type 1) diabetes but possible contribution by other parechoviruses is not ruled out. the aim was to compare children diagnosed with type 1 diabetes in 2005-2011 (n = 69) with healthy controls (n = 294), all from the jämtland county in sweden, using an exploratory suspension multiplex immunoassay for igm and igg against 26 peptides of lv, human parechoviruses (hpev), aichi virus and poliovirus in relation to a radiobinding as ... | 2015 | 25873230 |
| evidence of ljungan virus specific antibodies in humans and rodents, finland. | ljungan virus (lv, genus parechovirus, family picornaviridae) is considered currently to be a rodent-borne virus. despite suggested human disease associations, its zoonotic potential remains unclear. to date, lv antibody prevalence in both humans and rodents has not been studied. in this study, two different lv immunofluorescence assays (lv ifas) were developed with lv genotypes 1 (lv strain 87-012g) and 2 (lv strain 145slg), and cross-neutralization and -reaction studies were carried out with l ... | 2013 | 23852812 |
| isolation of a sp. nov. ljungan virus from wild birds in japan. | ljungan virus (lv) has been isolated/detected from rodents in a limited area including european countries and the usa. in this study, we isolated an lv strain from faecal samples of wild birds that had been collected in japan, and determined the nearly complete sequence of the genome. sequence analyses showed that the isolate possesses an lv-like genomic organization: 5utr-vp0-vp3-vp1-2a1-2a2-2b-2c-3a-3b-3c-3d-3utr. phylogenetic and similarity analyses based on the vp1 region indicated that the ... | 2016 | 27207304 |
| serological survey in the finnish human population implies human-to-human transmission of ljungan virus or antigenically related viruses. | ljungan virus (lv) is a picornavirus related to human parechoviruses (hpev). the virus has been found in bank voles (myodes glareolus) and several other rodent species, and suggested to have zoonotic potential. thus far, seroepidemiological data on lv infections in humans are scarce. in this study, we aimed to characterize the demographic and geographical distribution of lv-reactive antibodies in finland, and to investigate its occurrence in patients suspected of having a rodent-borne disease, n ... | 2016 | 26489898 |
| structure of ljungan virus provides insight into genome packaging of this picornavirus. | picornaviruses are responsible for a range of human and animal diseases, but how their rna genome is packaged remains poorly understood. a particularly poorly studied group within this family are those that lack the internal coat protein, vp4. here we report the atomic structure of one such virus, ljungan virus, the type member of the genus parechovirus b, which has been linked to diabetes and myocarditis in humans. the 3.78-å resolution cryo-electron microscopy structure shows remarkable featur ... | 2015 | 26446437 |
| genome characterisation of two ljungan virus isolates from wild bank voles (myodes glareolus) in sweden. | ljungan virus (lv) (family picornaviridae, genus parechovirus) is a suspected zoonotic pathogen with associations to human disease in sweden. lv is a single-stranded rna virus with a positive sense genome. there are five published ljungan virus strains, three isolated from sweden and two from america, and are classified into four genotypes. a further two strains described here were isolated from wild bank voles (myodes glareolus) caught in västmanlands county, sweden in 1994. these strains were ... | 2015 | 26375731 |
| evaluation of the association of zoonotic ljungan virus with perinatal deaths and fetal malformation. | more and more epidemiologic and experimental data support the notion that ljungan virus (lv), originally isolated from some rodent populations in sweden, denmark, and the united states, plays an important role in stillbirth and fetal malformation. mouse dams infected with lv may result in uterine resorption and perinatal deaths that may cross generations, and their offspring may suffer high rates of malformations including cranial, brain, and limb malformations. in humans, researches founded tha ... | 2015 | 25789980 |
| secondary structure analysis of swine pasivirus (family picornaviridae) rna reveals a type-iv ires and a parechovirus-like 3' utr organization. | the potential rna structures of the 5' and 3' untranslated regions (utrs) and cis-acting replication elements (cres) of a novel pasivirus (pav) genotype (family picornaviridae) were analysed. pav-a3 (km259923) was identified in a faecal sample from a domestic pig in hungary with posterior paraplegia of unknown etiology. based on likely structural features of the 5' utr, the pasiviruses were inferred to possess hepacivirus/pestivirus-like type-iv ires. the pasivirus cre was mapped to the 2b genom ... | 2015 | 25716922 |
| serological survey of rodent-borne viruses in finnish field voles. | in northern europe, rodent populations display cyclic density fluctuations that can be correlated with the human incidence of zoonotic diseases they spread. during density peaks, field voles (microtus agrestis) become one of the most abundant rodent species in northern europe, yet little is known of the viruses they host. we screened 709 field voles, trapped from 14 sites over 3 years, for antibodies against four rodent-borne, potentially zoonotic viruses or virus groups-hantaviruses, lymphocyti ... | 2014 | 24689532 |
| ljungan virus and an adenovirus in italian squirrel populations. | we report ljungan virus infection in eurasian red squirrels (sciurus vulgaris) for the first time, and extend the known distribution of adenoviruses in both native red squirrels and alien gray squirrels (sciurus carolinensis) to southern europe. | 2014 | 24484493 |
| identification of a novel picornavirus in healthy piglets and seroepidemiological evidence of its presence in humans. | in this study, we describe a novel porcine parechovirus-like virus (tentatively named plv-chn) from healthy piglets in china using 454 high-throughput sequencing. the complete genome of the virus comprises 6832 bp, encoding a predicted polyprotein of 2132 amino acids that is most similar to ljungan virus (32% identity). a similar virus that belongs to a novel picornaviridae genus, named swine pasivirus 1 (spav-1), was reported during the preparation of this paper. sequence analysis revealed that ... | 2013 | 23936384 |
| a link between ljungan virus and type 1 diabetes? | | 2013 | 23772973 |
| relationship between ljungan virus antibodies, hla-dq8, and insulin autoantibodies in newly diagnosed type 1 diabetes children. | environmental factors, including viral infections, may explain an increasing and fluctuating incidence of childhood type 1 diabetes (t1d). ljungan virus (lv) isolated from bank voles have been implicated, but it is unclear whether lv contributes to islet autoimmunity, progression to clinical onset, or both, of t1d. the aim was to test whether lv antibodies (lvab) were related to hla-dq and islet autoantibodies in newly diagnosed t1d patients (n=676) and controls (n=309). patients, 0-18 years of ... | 2013 | 23682738 |
| ljungan virus is endemic in rodents in the uk. | ljungan virus is a recently identified member of the family picornaviridae that was isolated from bank voles in sweden. ljv has been associated with [corrected] type 1 diabetes-like symptoms and myocarditis in bank voles (myodes glareolus), and it has been suggested that it has zoonotic potential. here, we show for the first time that ljungan virus is prevalent (20-27 % positive by pcr) in four species of uk rodent (myodes glareolus [bank vole], apodemus sylvaticus [wood mouse], microtus agresti ... | 2014 | 23665770 |
| [what should the veterinarian know about the ljungan virus]. | | 2013 | 23638555 |
| genomic characterization of sebokele virus 1 (sebv1) reveals a new candidate species among the genus parechovirus. | we determined the genomic features and the taxonomic classification of sebokele virus 1 (sebv1), a previously unclassified arbovirus isolated in 1972 from rodents collected in botambi, central african republic. the complete genome sequence was obtained using a deep sequencing approach (illumina technology) and dedicated bioinformatics workflows for data analysis. molecular analysis identified sebv1 as a picornavirus, most closely related to ljungan viruses of the genus parechovirus. the genome h ... | 2013 | 23596267 |
| ljungan/sebokele-like picornavirus in birds of prey, common kestrel (falco tinnunculus) and red-footed falcon (f. vespertinus). | ljungan and sebokele viruses are thought to be rodent-borne (picorna)viruses in the genus parechovirus. using random amplification and next generation sequencing method a novel ljungan/sebokele-like picornavirus was identified in birds of prey. viral rna was detected in total of 1 (9%) of the 11 and 2 (28.6%) of the 7 faecal samples from common kestrels and red-footed falcons in hungary, respectively. high faecal viral rna load (4.77×10(6) genomic copies/ml) measured by qpcr. the complete genome ... | 2017 | 28843546 |
| geographical distribution of ljungan virus in small mammals in europe. | ljungan virus (lv), which belongs to the parechovirus genus in the picornaviridae family, was first isolated from bank voles (myodes glareolus) in sweden in 1998 and proposed as a zoonotic agent. to improve knowledge of the host association and geographical distribution of lv, tissues from 1685 animals belonging to multiple rodent and insectivore species from 12 european countries were screened for lv-rna using reverse transcriptase (rt)-pcr. in addition, we investigated how the prevalence of lv ... | 2020 | 32487013 |
| effectiveness of antivirals in a type 1 diabetes model and the move toward human trials. | a picornavirus (ljungan virus [lv]) originally found in bank voles has been associated with type 1 diabetes (t1d) in its wild rodent reservoir, but also associated with t1d in a laboratory rat model for the disease, the diabetes prone (dp) bio breeding (bb) rat. successful treatment of diabetes in this rat model, using experimental antiviral compounds directed against picornavirus, has been reported. in the present study we show significant clinical response in dp-bb rats using antiviral compoun ... | 2020 | 32758075 |
| evolutionary relationships of ljungan virus variants circulating in multi-host systems across europe. | the picornavirus named 'ljungan virus' (lv, species parechovirus b) has been detected in a dozen small mammal species from across europe, but detailed information on its genetic diversity and host specificity is lacking. here, we analyze the evolutionary relationships of lv variants circulating in free-living mammal populations by comparing the phylogenetics of the vp1 region (encoding the capsid protein and associated with lv serotype) and the 3dpol region (encoding the rna polymerase) from 24 ... | 2021 | 34372523 |
| characterizing the picornavirus landscape among synanthropic nonhuman primates in bangladesh, 2007 to 2008. | the term synanthropic describes organisms that thrive in human-altered habitats. where synanthropic nonhuman primates (nhp) share an ecological niche with humans, cross-species transmission of infectious agents can occur. in bangladesh, synanthropic nhp are found in villages, densely populated cities, religious sites, and protected forest areas. nhp are also kept as performing monkeys and pets. to investigate possible transmission of enteric picornaviruses between humans and nhp, we collected fe ... | 2013 | 23097448 |
| distribution and seasonal variation of ljungan virus in bank voles (myodes glareolus) in fennoscandia. | ljungan virus (lv) is a picornavirus originally isolated from swedish bank voles ( myodes glareolus ) in 1998. the association of lv with human disease has been debated ever since, but fundamental data on the ecology of the virus are still lacking. here we present results of the first intensive study on the prevalence of lv in bank voles trapped in fennoscandia (sweden and finland) from 2009-2012 as determined by pcr. using an lv-specific rt-pcr, lv was detected in the liver of 73 out of 452 (16 ... | 2017 | 28192046 |
| islet autoantibodies present in association with ljungan virus infection in bank voles (myodes glareolus) in northern sweden. | bank voles are known reservoirs for puumala hantavirus and probably also for ljungan virus (lv), a suggested candidate parechovirus in type 1 diabetes etiology and pathogenesis. the aim of this study was to determine whether wild bank voles had been exposed to lv and if exposure associated to autoantibodies against insulin (iaa), glutamic acid decarboxylase 65 (gada), or islet autoantigen-2 (ia-2a). serum samples from bank voles (myodes glareolus) captured in early summer or early winter of 1997 ... | 2017 | 27283793 |
| lymphocytic choriomeningitis, ljungan and orthopoxvirus seroconversions in patients hospitalized due to acute puumala hantavirus infection. | the emergence and re-emergence of zoonotic and vector-borne diseases are increasing in europe. prominent rodent-borne zoonotic viruses include puumala hantavirus (puuv; the causative agent of nephropathia epidemica, ne), lymphocytic choriomeningitis virus (lcmv), and orthopoxviruses (opv). in addition, ljungan virus (lv) is considered a potentially zoonotic virus. | 2016 | 27721109 |
| diabetes prevention through antiviral treatment in biobreeding rats. | a picornavirus (ljungan virus) has been associated with diabetes in its wild rodent reservoir and in diabetes-prone biobreeding (dp-bb) rats. we attempted to alter the development of diabetes in dp-bb rats using two anti-picornavirus compounds (pleconaril and apo-n039), singly or in combination. antiviral therapy was initiated 2 weeks before expected onset of diabetes. pleconaril or apo-n039 alone did not affect the debut of diabetes. however, animals receiving a combination of both compounds we ... | 2016 | 27505258 |
| extending the enterovirus lead: could a related picornavirus be responsible for diabetes in humans? | we found an association between the abundance of rodents in the wild and onset of type 1 diabetes (t1d) in humans. a picornavirus named ljungan virus (lv) was subsequently isolated from wild bank voles. both picornavirus-like particles detected by electron microscopy and lv antigen visualized by immunohistochemistry was seen in islets of langerhans in diabetic wild bank voles. lv antigen has also been found in islets of langerhans in a patient with recent onset of t1d and in the commonly used bi ... | 2020 | 32927606 |
| neutralizing ljungan virus antibodies in children with newly diagnosed type 1 diabetes. | ljungan virus (lv), a parechovirus of the picornavirus family, first isolated from a bank vole at the ljungan river in sweden, has been implicated in the risk for autoimmune type 1 diabetes. an assay for neutralizing ljungan virus antibodies (nlva) was developed using the original 87-012 lv isolate. the goal was to determine nlva titres in incident 0-18 years old newly diagnosed type 1 diabetes patients (n=67) and school children controls (n=292) from jämtland county in sweden. nlva were found i ... | 2021 | 34020728 |