| identification and molecular cloning of a novel porcine parvovirus. | a novel porcine parvovirus, ppv4, was identified in the lung lavage of a diseased pig coinfected with porcine circovirus type 2. this virus exhibits limited similarity to its closest relative, bovine parvovirus 2, but resembles viruses of the genus bocavirus (bovine parvovirus, canine minute virus and human bocavirus) that encode an additional orf3. the orf3 of ppv4 is predicted to encode a protein of 204 amino acid residues, which is similar in size to the orf3-encoded proteins of the bocavirus ... | 2010 | 20339886 |
| detection of a novel porcine parvovirus, ppv4, in chinese swine herds. | to determine whether the novel porcine parvovirus type 4 (ppv4) recently reported in america is prevalent in china, a set of specific primers was designed and used for molecular survey of ppv4 among the clinical samples collected from various provinces of china between 2006 and 2010. the results showed that ppv4 is present in chinese swine herds at a rate of 2.09% (12/573) among the clinical samples examined and 0.76% (1/132) among the samples taken from healthy animals. we also noted that ppv4 ... | 2010 | 21092136 |
| a metagenomics and case-control study to identify viruses associated with bovine respiratory disease. | bovine respiratory disease (brd) is a common health problem for both dairy and beef cattle, resulting in significant economic loses. in order to identify viruses associated with brd, we used a metagenomics approach to enrich and sequence viral nucleic acids in the nasal swabs of 50 young dairy cattle with symptoms of brd. following deep sequencing, de novo assembly, and translated protein sequence similarity searches, numerous known and previously uncharacterized viruses were identified. bovine ... | 2015 | 25740998 |
| characterization of a novel porcine parvovirus tentatively designated ppv5. | a new porcine parvovirus (ppv), provisionally designated as ppv5, was identified in u.s. pigs. cloning and sequencing from a circular or head-to-tail concatemeric array revealed that the ppv5 possesses the typical genomic organization of parvoviruses with two major predicted open reading frames (orf1 and orf2), and is most closely related to ppv4 with overall genomic identities of 64.1-67.3%. the amino acid identities between ppv5 and ppv4 were 84.6%-85.1% for orf1 and 54.0%-54.3% for orf2. unli ... | 2013 | 23762339 |
| ungulate copiparvovirus 1 (bovine parvovirus 2): characterization of a new genotype and associated viremia in different bovine age groups. | a novel bovine parvovirus 2 (bpv2) genotype comprising 5394 nt was identified by next generation sequencing from sera of healthy cattle at different age groups farmed in the state of rio grande do sul, brazil. the genome organization of new bpv2 genotype retains the two orfs typical of members of the parvovirinae with 86.4 % of overall nucleotide sequence identities in comparison to other members of the subfamily. phylogenetic analysis revealed similar clustering with two previously described bo ... | 2016 | 26646894 |
| virome of us bovine calf serum. | using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the united states. two parvoviruses, bovine parvovirus 2 (bpv2) and a previously uncharacterized parvovirus designated as bosavirus (bosav), were detected in 3 and 4 pools respectively and their complete coding sequences generated. based on ns1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. also detected were low number of reads matching ungulate tetraparvovi ... | 2017 | 28100412 |