| infectivity of the cloned components of a begomovirus: dna beta complex causing chilli leaf curl disease in india. | the full-length genome of a begomovirus and its cognate dna-beta satellite component associated with chilli leaf curl disease (chlcd), originating from varanasi, india, were cloned. sequence analysis revealed that the viral genome (ef190217) is 2,750 bp and the dna-beta satellite (ef190215) is 1,361 bp in length. agroinoculation with partial tandem repeats of the viral genome along with the satellite induced symptoms typical of chlcd in chilli and nicotiana benthamiana. however, symptom expressi ... | 2008 | 18175042 |
| chili leaf curl betasatellite is associated with a distinct recombinant begomovirus, pepper leaf curl lahore virus, in capsicum in pakistan. | capsium spp. are an important vegetable crop cultivated through pakistan. leaf curl disease is the major disease of capsicum spp. in pakistan caused by viruses. the disease has previously been shown to be associated with begomoviruses and betasatellites. we have cloned and sequenced a begomovirus and its associated betasatellite from capsicum originating from central pakistan. the begomovirus isolated was distinct from all previously characterised viruses and we propose the name pepper leaf curl ... | 2010 | 20079779 |
| an improved plant regeneration and agrobacterium - mediated transformation of red pepper (capsicum annuum l.). | capsicum annuum (red pepper) is an important spice cum vegetable crop in tropical and subtropical countries. here, we report an effective and reproducible auxin free regeneration method for six different red pepper cultivars (aca-10, kashi anmol, lca-235, pbc-535, pusa jwala and supper) using hypocotyl explants and an efficient agrobacterium-mediated transformation protocol. the explants (hypocotyls, cotyledonary leaves and leaf discs) collected from axenic seedlings of six red pepper cultivars ... | 2012 | 24082498 |
| chilli leaf curl virus-based vector for phloem-specific silencing of endogenous genes and overexpression of foreign genes. | geminiviruses are the largest and most devastating group of plant viruses which contain ssdna as a genetic material. geminivirus-derived virus-induced gene silencing (vigs) vectors have emerged as an efficient and simple tool to study functional genomics in various plants. however, previously developed vigs vectors have certain limitations, owing to their inability to be used in tissue-specific functional study. in the present study, we developed a chilli leaf curl virus (chilcv)-based vigs vect ... | 2017 | 27878582 |
| characterization of a new begomovirus and betasatellite associated with chilli leaf curl disease in india. | chilli leaf curl disease (chilcd) is a serious problem and a major limitation to chilli (capsicum spp.) cultivation in india. leaves of a chilli plant showing leaf curl symptoms were collected from the gonda district in uttar pradesh, india, in april, 2013. full-length genomes of a begomovirus and an associated betasatellite were amplified, cloned and sequenced. the size of the begomovirus genome and the betasatellite were 2760 bp and 1374 bp, respectively. the nucleotide sequence of the begomov ... | 2017 | 27738844 |
| identification and molecular characterization of a new recombinant begomovirus and associated betasatellite dna infecting capsicum annuum in india. | capsicum annuum (chilli) is a perennial herbaceous plant that is cultivated as an annual crop throughout the world, including india. chilli leaf curl disease (chilcd) is a major biotic constraint, causing major losses in chilli production. during 2014, leaf samples of chilli plants displaying leaf curl disease were collected from the ahmedabad district of gujarat, india. these samples were used to isolate, clone and sequence viral genomic dna and an associated betasatellite dna molecule. sequenc ... | 2016 | 26831933 |
| synergistic interaction among begomoviruses leads to the suppression of host defense-related gene expression and breakdown of resistance in chilli. | chilli (capsicum sp.) is one of the economically important spice and vegetable crops grown in india and suffers great losses due to the infection of begomoviruses. conventional breeding approaches have resulted in development of a few cultivars of chilli resistant to begomoviruses. a severe leaf curl disease was observed on one such resistant chilli cultivar (capsicum annuum cv. kalyanpur chanchal) grown in the experimental field of the jawaharlal nehru university, new delhi. four different vira ... | 2016 | 26780359 |
| rnai mediated broad-spectrum transgenic resistance in nicotiana benthamiana to chilli-infecting begomoviruses. | two rnai constructs were designed targeting chilli-infecting begomoviruses and associated betasatellites. broad-spectrum resistance was achieved against multiple begomoviruses associated with leaf curl disease of chillies in india. chilli leaf curl disease (chilcd) caused by begomoviruses (family: geminiviridae) has emerged as one of the most devastating viral diseases of chilli, especially in the indian sub-continent. the severity of disease incidence is expanding at an alarming rate due to the ... | 2015 | 25916177 |
| chilli leaf curl virus infection highlights the differential expression of genes involved in protein homeostasis and defense in resistant chilli plants. | geminiviruses have evolved with tremendous potential of recombination and possess the ability to manipulate several cellular processes of hosts. chilli leaf curl virus (chilcv) is a monopartite begomovirus (family geminiviridae) which has emerged as a serious threat to chilli production worldwide. to date, development of resistant chilli varieties through conventional plant breeding techniques remains the major antiviral strategy. to explore the potential resistance factors in capsicum annuum va ... | 2015 | 25693670 |
| molecular characterization of chilli leaf curl viruses infecting new host plant petunia hybrida in india. | petunia hybrida is an important ornamental plant grown in many countries including india. it is a good model plant for the study of genetics and molecular biology. during a survey in 2013-2014, severe leaf curling was observed on most of the p. hybrida grown in the sikar district, rajasthan. the infected plants were analyzed for begomovirus infection by rolling circular amplification (rca) and sequenced. full length sequences confirmed the association of monopartite begomovirus with betasatellit ... | 2015 | 25294775 |
| molecular characterization of chilli leaf curl virus and satellite molecules associated with leaf curl disease of amaranthus spp. | amaranthus, collectively known as amaranth, is an annual or short-lived perennial plant used as leafy vegetables, cereals and for ornamental purposes in many countries including india. during 2011, leaf samples of amaranthus plants displaying leaf curling, leaf distortion, leaf crinkling and yellow leaf margins were collected from banswara district, rajasthan in india. full-length clones of a monopartite begomovirus, a betasatellite and an alphasatellite were characterized. the complete nucleoti ... | 2014 | 24368759 |
| recent evolution of a novel begomovirus causing tomato leaf curl disease in the al-batinah region of oman. | for last two decades, begomoviruses (family geminiviridae) have been a major constraint for tomato production in oman, particularly in the al-batinah region, the major agricultural area of oman. farms in the al-batinah region were surveyed during january-march and november-december in 2012 and january-february in 2013. leaf samples of tomato plants showing typical leaf curl disease symptoms were collected and analyzed for begomoviruses. out of fifteen begomovirus clones sequenced, seven were sho ... | 2014 | 24052149 |
| genetic diversity and distribution of a distinct strain of chili leaf curl virus and associated betasatellite infecting tomato and pepper in oman. | tomato and pepper are widely grown in oman for local consumption. a countrywide survey was conducted during 2010-2011 to collect samples and assess the diversity of begomoviruses associated with leaf curl disease of tomato and pepper. a virus previously only identified on the indian subcontinent, chili leaf curl virus (chlcv), was found associated with tomato and pepper diseases in all vegetable grown areas of oman. some of the infected plant samples were also found to contain a betasatellite. a ... | 2013 | 23911631 |
| spatial and temporal diversity of begomoviral complexes in papayas with leaf curl disease. | old world, monopartite begomoviruses associated with satellite dna β were observed in papaya showing symptoms of leaf curl disease sampled randomly over five years from within a radius of 250 km in north-central india. three groups of dna a sequences were evident. one group resembled chili leaf curl virus infecting tomatoes (chilcuv). another group resembled tomato leaf curl new delhi virus (tolcundv). the third group was novel (tentatively named papaya leaf crumple virus, palcrv), with less tha ... | 2012 | 22437254 |
| complete nucleotide sequence of chili leaf curl virus and its associated satellites naturally infecting potato in pakistan. | | 2009 | 19123046 |
| the replication initiator protein of a geminivirus interacts with host monoubiquitination machinery and stimulates transcription of the viral genome. | geminiviruses constitute a group of plant viruses, with a ssdna genome, whose replication in the nucleus of an infected cell requires the function of geminivirus-encoded replication initiator protein (rep). our results suggest that monoubiquitinated histone 2b (h2b-ub) promotes tri-methylation of histone 3 at lysine 4 (h3-k4me3) on the promoter of chilli leaf curl virus. we isolated homologues of two major components of the monoubiquitination machinery: ubiquitin-conjugating enzyme2 (nbubc2) and ... | 2017 | 28859169 |