| molecular analysis of bacterial communities in uranium ores and surrounding soils from banduhurang open cast uranium mine, india: a comparative study. | bacterial community structure of heavy metal rich- uranium ores and surrounding soils was explored using 16s rrna gene based clone library analysis and denaturing gradient gel electrophoresis (dgge) to provide baseline microbial diversity data on autochthonous communities. sequence analysis of major ribotypes and/or dgge bands revealed proteobacteria and acidobacteria as the two most frequently present bacterial phyla across the samples, although relative abundance of each phyla and identity of ... | 2011 | 21308598 |
| [effects of 2-chlorophenol-acclimation on microbial community structure in anaerobic granular sludge]. | the microbial community structure in 2-chlorophenol-acclimated anaerobic granular sludge and inoculating sludge were analyzed by 16s rdna-based approach. total dna was extracted directly from the inoculating sludge and 2-cp-acclimated anaerobic sludge, and then amplified by polymerase chain reaction (pcr) technique with the specific primer pair arc21f/arc958r for archaea and 31f/907r for acidobacteria respectively. the positive pcr products were cloned and sequenced. the sequences analysis shows ... | 2008 | 18649544 |
| spatial distribution of 16s rrna levels from uncultured acidobacteria in soil. | the activity of uncultured acidobacteria was monitored in dutch grassland soils by quantifying their ribosomes. these bacteria were detectable by five different 16s rrna rt-pcr products in temperature gradient gel electrophoresis fingerprints. the ribosomes in surface soil samples were quantified with multiple competitive rt-pcr along a 1.5-km transect through the grassland. in total, the five members of the acidobacteria were estimated to contribute 4 x 1010 to 1 x 1011 ribosomes g soil-1, repr ... | 2000 | 10972712 |