| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| inquilinus limosus in patients with cystic fibrosis, germany. | we identified inquilinus limosus, a recently described ?-proteobacterium, in sputum of 2 patients with cystic fibrosis whose respiratory tracts were persistently colonized for >9 months. we present data on the epidemiology, antimicrobial susceptibility, and molecular characteristics of i. limosus. | 2005 | 15757565 |
| inquilinus limosus isolated from a cystic fibrosis patient: first uk report. | | 2007 | 17910283 |
| inquilinus limosus and cystic fibrosis. | | 2008 | 18507928 |
| molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients. | there is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (cf) patients. | 2008 | 18682840 |
| low rates of pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients. | pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (cf). its correct identification ensures effective patient management and infection control strategies. however, little is known about how often cf sputum isolates are falsely identified as p. aeruginosa. we used p. aeruginosa-specific duplex real-time pcr assays to determine if 2,267 p. aeruginosa sputum isolates from 561 cf patients were correctly identified by 17 australian clinical microbiology laboratorie ... | 2009 | 19261796 |
| mucoid inquilinus limosus in a young adult with cystic fibrosis. | inquilinus limosus is a alpha-proteobacterium that has been recently isolate in the airways of cystic fibrosis (cf) patients. we report the isolation of a mucoid strain of i. limosus from the sputum of a 20-year-old male patient with cf over 1 year that was associated with the clinical, spirometric, and radiographic decline in a previously healthy patient. | 2009 | 19434691 |
| eurocarecf quality assessment of diagnostic microbiology of cystic fibrosis isolates. | the identification of microbial species from respiratory specimens and their susceptibility to antimicrobial agents are among the most important diagnostic measures of care for patients with cystic fibrosis (cf). under the umbrella of eurocarecf, two quality assurance trials of cf microbiology were performed in 2007 and 2008. nine formulations with cf bacterial isolates were dispatched. a total of 31/37 laboratories from 18/21 european countries participated in the 2007 and 2008 trials. the comm ... | 2009 | 19741077 |
| antibiotic resistance of mixed biofilms in cystic fibrosis: impact of emerging microorganisms on treatment of infection. | cystic fibrosis (cf) is a genetic disorder associated with multispecies infections where interactions between classical and newly identified bacteria might be crucial to understanding the persistent colonisation in cf lungs. this study investigated the interactions between two emerging species, inquilinus limosus and dolosigranulum pigrum, and the conventional cf pathogen pseudomonas aeruginosa by evaluating the ability to develop biofilms of mixed populations and then studying their susceptibil ... | 2012 | 22770521 |
| insights into cystic fibrosis polymicrobial consortia: the role of species interactions in biofilm development, phenotype, and response to in-use antibiotics. | cystic fibrosis (cf) airways disease involves complex polymicrobial infections where different bacterial species can interact and influence each other and/or even interfere with the whole community. to gain insights into the role that interactions between pseudomonas aeruginosa in co-culture with staphylococcus aureus, inquilinus limosus, and stenotrophomonas maltophilia may play in infection, the reciprocal effect during biofilm formation and the response of dual biofilms toward ciprofloxacin u ... | 2016 | 28133457 |
| inquilinus limosus in pulmonary disease: case report and review of the literature. | inquilinus limosus is a slow growing, gram-negative, oxidase-positive, non-fermentative bacillus that is rarely isolated from clinical samples. when clinically identified, i. limosus is almost exclusively isolated from the respiratory tracts of patients with cystic fibrosis (cf). we report the first case of i. limosus isolation from a pulmonary specimen in an individual without a diagnosis of cf. a review of the english-language literature has been made and shows 33 cases (excluding the present ... | 2016 | 27717651 |
| conformational properties of two exopolysaccharides produced by inquilinus limosus, a cystic fibrosis lung pathogen. | inquilinus limosus is a multi-resistant bacterium found in the respiratory tract of patients with cystic fibrosis. this bacterium produces two unique fully pyruvylated exopolysaccharides in similar quantities: an α-(1→2)-linked mannan and a β-(1→3)-linked glucan. we employed molecular modelling methods to probe the characteristic conformations and dynamics of these polysaccharides, with corroboration from potentiometric titrations and circular dichroism experiments. our calculations reveal diffe ... | 2012 | 22261278 |
| chronic colonization of inquilinus limosus in a patient with cystic fibrosis: first report in spain. | | 2013 | 23352213 |
| continuous infusion meropenem and ticarcillin-clavulanate in pediatric cystic fibrosis patients. | aztreonam, cefepime, and ceftazidime are anti-pseudomonal beta-lactam antibiotics which have been previously reported to be administered by continuous infusion (ci) in pediatric cf patients. we present two cases administering intravenous (iv) meropenem and ticarcillin-clavulanate by ci in pediatric cf patients. the delivery of beta-lactam antibiotics via ci should be considered in order to optimize the pharmacodynamics (pd) of beta-lactams in the treatment of acute pulmonary exacerbations (ape). | 2014 | 23775821 |
| inq-1, a chromosome-encoded ampc β-lactamase from inquilinus limosus. | | 2014 | 24072168 |
| identification of inquilinus limosus in cystic fibrosis: a first report in italy. | cystic fibrosis is a genetic disorder associated with a polymicrobial lung infection where classical pathogens and newly identified bacteria may interact. inquilinus limosus is an a-proteobacterium recently isolated in the airways of cystic fibrosis patient. we report the first case in italy of i.limosus isolation from the sputum sample of a cystic fibrosis patient. the patient is a 20-years-old man with cystic fibrosis, regularly attending the regional care center for cystic fibrosis at the fed ... | 2014 | 25387296 |
| bacteremic inquilinus limosus empyema in an australian lung transplant patient with cystic fibrosis. | | 2015 | 26267744 |
| draft genome sequence of inquilinus limosus strain mp06, a multidrug-resistant clinical isolate. | the bacterium, inquilinus limosus, with its remarkable antimicrobial multiresistant profile, has increasingly been isolated in cystic fibrosis patients. we report draft genome sequence of a strain mp06, which is of considerable interest in elucidating the associated mechanisms of antibiotic resistance in this bacterium and for an insight about its persistence in airways of these patients. | 2016 | 26691451 |
| discriminating typical and atypical cystic fibrosis-related bacteria by multiplex pna-fish. | this study aims to report the development of peptide nucleic acid (pna) probes to specifically detect the cystic fibrosis (cf)-associated traditional and atypical species pseudomonas aeruginosa and inquilinus limosus, respectively. pna probes were designed in silico, developed and tested in smears prepared in phosphate-buffer saline (pbs), and in artificial sputum medium (asm). a multiplex fluorescent in situ hybridization (fish) approach using the designed probes was further validated in artifi ... | 2017 | 27571488 |
| emergent bacteria in cystic fibrosis: in vitro biofilm formation and resilience under variable oxygen conditions. | concurrent to conventional bacterial pathogens, unusual microbes are emerging from cystic fibrosis (cf) airways. nonetheless, little is known about the contribution of these newly microbes to the resilience of cf-associated biofilms, particularly under variable-oxygen concentrations that are known to occur in vivo in the mucus of cf patients. two cf-emergent bacterial species, inquilinus limosus and dolosigranulum pigrum, and the major pathogen pseudomonas aeruginosa were studied in terms of bio ... | 2014 | 24868541 |
| the use of doripenem in pediatric cystic fibrosis patients in case of meropenem shortages. | ceftazidime is the only anti-pseudomonal beta-lactam that has been reported to be administered by extended infusion in pediatric cystic fibrosis (cf) patients. a small pediatric pharmacokinetic/pharmacodynamic study has been published regarding the use of intermittent extended infusion doripenem in the treatment of an acute pulmonary exacerbation (ape) in pediatric cf patients; however, clinical use of intermittent extended infusion doripenem in pediatric cf patients has not been previously repo ... | 2014 | 23661625 |
| inquilinus ginsengisoli sp. nov., isolated from soil of a ginseng field. | a gram-reaction-negative, chemo-organotrophic, non-motile, non-spore-forming, rod-shaped bacterium (strain gsoil 080(t)) was isolated from soil collected in a ginseng field in pocheon province, south korea, and was investigated by using a polyphasic taxonomic approach. comparative 16s rrna gene sequence analysis showed that strain gsoil 080(t) was related most closely to inquilinus limosus strains au0476(t) and au1979 (98.9 % similarity to both). strain gsoil 080(t) shared ≤91.3 % 16s rrna gene ... | 2011 | 20190024 |
| dongia mobilis gen. nov., sp. nov., a new member of the family rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent. | a gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated lm22(t), was isolated from activated sludge of a sequencing batch reactor for the treatment of malachite green effluent. cells of strain lm22(t) were slightly curved to straight rods (0.3-0.5×0.6-1.0μm) and motile by a single polar flagellum. strain lm22(t) was negative for oxidase and catalase activities and phototrophic growth. an internal membrane system and bacteriochlorophyll a were absent. g ... | 2010 | 20061488 |
| inhibition of cathelicidin activity by bacterial exopolysaccharides. | the interaction of bacterial exopolysaccharides, produced by opportunistic lung pathogens, with antimicrobial peptides of the innate primate immune system was investigated. the exopolysaccharides were produced by pseudomonas aeruginosa, inquilinus limosus and clinical isolates of the burkholderia cepacia complex, bacteria that are all involved in lung infections of cystic fibrosis patients. the effects of the biological activities of three orthologous cathelicidins from homo sapiens sapiens, pon ... | 2009 | 19400793 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| exopolysaccharides produced by inquilinus limosus, a new pathogen of cystic fibrosis patients: novel structures with usual components. | the major cause of morbidity and mortality in patients with cystic fibrosis, an autosomal recessive disorder, is chronic microbial colonisation of the major airways that leads to exacerbation of pulmonary infection. several different microbes colonise cystic fibrosis lungs, and pseudomonas aeruginosa is one of the most threatening, since the establishment of mucoid (alginate producing) strains is ultimately associated with the patient's death. very recently a new bacterium, named inquilinus limo ... | 2007 | 17719019 |
| identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india. | lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ... | 2007 | 17264993 |
| clonal analysis of inquilinus limosus isolates from six cystic fibrosis patients and specific serum antibody response. | inquilinus limosus is a novel gram-negative bacterium of the subdivision alpha-proteobacteria recently found in the airways of patients with cystic fibrosis (cf). here, the authors report on the clinical courses of six cf patients colonized with i. limosus. five patients suffered from either an acute respiratory exacerbation or a progressive loss of pulmonary function, whereas one patient was in a stable clinical situation. this study focused on two aims: (i) the clonal analysis of i. limosus is ... | 2006 | 17005793 |