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occurrence of natural dixenic associations between the symbiont photorhabdus luminescens and bacteria related to ochrobactrum spp. in tropical entomopathogenic heterorhabditis spp. (nematoda, rhabditida).bacteria naturally associated with the symbiont photorhabdus luminescens subsp. akhurstii were isolated from the entomopathogenic nematode heterorhabditis indica. bacterial isolates distinct from p. luminescens subsp. akhurstii were obtained from 33% of the samples. fourteen bacterial isolates, from nematodes collected from three different caribbean islands, were characterized by conventional phenotypic tests, restriction fragment length polymorphism and sequence analyses of pcr-amplified 16s rr ...200010746775
isolation and characterization of the native entomopathogenic nematode, heterorhabditis brevicaudis, and its symbiotic bacteria from taiwan.the occurrence of heterorhabditis brevicaudis (rhabditida: heterorhabditidae) and its symbiotic bacteria, photorhabdus luminescens subsp. akhurstii in taiwan were recorded for the first time. h. brevicaudis was described by liu in 1994, but it was unavailable and no molecular data has ever been published for it ever since. the native entomopathogenic nematode (epn), h. brevicaudis tg01 was recovered from sandy coastal soils in moist bamboo forest, as observed in this article. the bacterial symbi ...200919214629
insecticidal toxic proteins produced by photorhabdus luminescens akhurstii, a symbiont of heterorhabditis indica.we describe the isolation and characterization of an insect pathogenic bacterium from the entomopathogenic nematode heterorhabditis indica (karnataka strain), an isolate from the southern regions of india. the strain has been identified and characterized by phenotypic, biochemical tests and pcr-rflp analysis of the 16s rrna gene as photorhabdus luminescens subsp. akhurstii. the insecticidal toxin complex produced by this bacterium has been purified through a series of steps including ultrafiltra ...200219265903
identification and characterization of a novel gene involved in the trans-specific nematicidal activity of photorhabdus luminescens ln2.photorhabdus luminescens subsp. akhurstii ln2 from heterorhabditis indica ln2 showed nematicidal activity against axenic heterorhabditis bacteriophora h06 infective juveniles (ijs). transposon mutagenesis identified an ln2 mutant that supports the growth of h06 nematodes. tn5 disrupted the nama gene, encoding a novel 364-residue protein and involving the nematicidal activity. the green fluorescent protein-labeled nama mutant was unable to colonize the intestines of h06 ijs.200919376907
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