| experimental mycoplasmal pneumonia in dogs: electron microscopy of infected tissue. | thin sections of lung tissue from dogs with pneumonia induced by endobronchial inoculation of mycoplasma cynos were examined by electron microscopy. mycoplasmas were observed extracellularly in lumen of bronchus and in alveoli in the earlier stages of the infection. the infection also resulted in degenerative changes in the bronchial epithelial cells, including in particular destruction and loss of cilia. | 1977 | 605806 |
| canine mycoplasmas: pathogenicity of mycoplasmas associated with distemper pneumonia. | mycoplasmas of the following species were isolated from the lungs of dog with distemper: mycoplasma cynos, mycoplasma bovigenitalium, mycoplasma canis, mycoplasma spumans, and mycoplasma gateae. one-week-old dogs were inoculated endobronchially with cloned cultures of each species. m. cynos induced a focal pneumonia that were characterized histologically by severe inflammation of bronchi and adjacent respiratory tract tissue. the inflammatory response was initially neutrophilic and later was his ... | 1978 | 681796 |
| mycoplasmas associated with canine infectious respiratory disease. | canine infectious respiratory disease (cird) is a complex infection that occurs worldwide predominantly in kennelled dogs, and several bacterial and viral micro-organisms have been associated with outbreaks of cird. however, few studies have comprehensively examined the species of mycoplasma present in healthy dogs and those with cird. as part of an extensive study investigating the micro-organisms involved in cird, the species of mycoplasma present throughout the respiratory tract of dogs with ... | 2004 | 15470126 |
| canine mycoplasmas. | this review aims to summarise our current understanding of the role of mycoplasmas in domestic dogs. canine mycoplasmology is a small field, with less than 50 publications in the past 40 years. in this time we have gained knowledge about the number of species and have made associations with infections in dogs. however much evidence is still lacking. the importance of all canine mycoplasmas remains unknown, yet certain species are associated with canine anaemia (mycoplasma haemocanis), respirator ... | 2004 | 15894017 |
| serological evidence of mycoplasma cynos infection in canine infectious respiratory disease. | a high proportion of dogs suffer from respiratory disease when they are placed in kennels for vacation or re-homing. the role of mycoplasma cynos as an initiating agent in canine infectious respiratory disease was investigated by examining the serological response of dogs to this organism at the time of entry into a large re-homing kennel. forty-two paired serum samples from dogs (21-day interval) were examined for antibody to m. cynos using western blotting. the development of antibody in the s ... | 2007 | 17184934 |
| simultaneous canine distemper virus, canine adenovirus type 2, and mycoplasma cynos infection in a dog with pneumonia. | the present case is the first description of a triple infection with canine distemper virus (cdv), canine adenovirus (cav) type 2, and mycoplasma cynos in a dog. the 5-month-old female miniature pinscher was euthanized because of dyspnea, croaking lung sounds, weight loss, and lymphopenia. pathologic examination revealed a fibrinous necrotizing pneumonia with large amphophilic intranuclear and acidophilic intracytoplasmatic inclusion bodies in different lung cells. immunohistochemically, cdv ant ... | 2007 | 17606512 |
| lethal bronchopneumonia caused by mycoplasma cynos in a litter of golden retriever puppies. | | 2007 | 17982143 |
| strain typing of mycoplasma cynos isolates from dogs with respiratory disease. | the association of mycoplasma cynos with canine infectious respiratory disease is increasingly being recognised. this study describes the strain typing of 14 m. cynos isolates cultured from trachea and bronchoalveolar lavage samples of six dogs with respiratory disease, from two separate kennels in the united kingdom. the genetic similarity of the isolates was investigated using pulsed-field gel electrophoresis (pfge) and random amplified polymorphic dna (rapd). most of the isolates from four do ... | 2009 | 18977617 |
| secreted sialidase activity of canine mycoplasmas. | through a survey of the phylogenetic distribution of sialidase among mycoplasmas, we detected activity secreted by the type strains of 3 of 11 species frequently or first isolated from dogs. the specific activity of washed cells of the type strains of mycoplasma canis, mycoplasma cynos, and mycoplasma molare ranged from 5.2+/-0.8 x 10(-6) to 1.1+/-0.3 x 10(-5) enzymatic units per colony-forming unit (u/cfu). cells of m. molare strain h542(t) had twice the specific activity (p<0.05) of m. canis s ... | 2009 | 19201110 |
| detection of bacterial and viral organisms from the conjunctiva of cats with conjunctivitis and upper respiratory tract disease. | a variety of pathogens are involved in conjunctivitis in cats. in this study, the prevalence of feline herpesvirus (fhv), chlamydophila felis, mycoplasmas, and aerobic bacteria on the conjunctival surface of cats with conjunctivitis and upper respiratory tract disease was investigated by polymerase chain reaction (pcr), immunofluorescent assay (ifa), and aerobic bacterial culture of ocular swabs. forty-one cats were included of which 37 were found to be infected with an ocular organism. single a ... | 2010 | 20817584 |
| demonstration of neuraminidase activity in mycoplasma neurolyticum and of neuraminidase proteins in three canine mycoplasma species. | neuraminidases are virulence factors in many pathogenic microorganisms. they are present also in some mycoplasma species that cause disease in birds, dogs and alligators. thirty-seven mycoplasma species have been examined previously for neuraminidase (sialidase) activity, whereas many of the species causing disease in man, ruminants, pigs, rodents and other animals have not. in this study neuraminidase enzymatic activity (neac) was examined in 45 previously untested mycoplasma species, including ... | 2011 | 21937171 |
| quantitative pcr and cytology of bronchoalveolar lavage fluid in dogs with bordetella bronchiseptica infection. | the use of quantitative pcr (qpcr) for detection of bordetella bronchiseptica in bronchoalveolar lavage fluid (balf) and demonstration of bacteria adhering to ciliated epithelial cells in balf or bronchial brushing fluid (bbf) has not been assessed in a series of affected dogs. coinfections can worsen the clinical severity in bordetellosis, but the specific association with mycoplasma cynos has not been evaluated. | 2016 | 27461723 |
| factors associated with development of canine infectious respiratory disease complex (cirdc) in dogs in 5 canadian small animal clinics. | this study investigated the association between presence of respiratory pathogens and development of canine infectious respiratory disease complex (cirdc) in dogs in 5 canadian small animal clinics. in total, 86 dogs were tested using a commercial pcr respiratory panel; 64 dogs were considered as cases and 22 were control dogs matched by veterinary clinic. no control animals (0/22) were positive for canine parainfluenza virus (cpiv), whereas 27/64 (42%) cirdc cases were positive. furthermore, 81 ... | 2016 | 26740697 |
| the detection and differentiation of canine respiratory pathogens using oligonucleotide microarrays. | canine respiratory diseases are commonly seen in dogs along with co-infections with multiple respiratory pathogens, including viruses and bacteria. virus infections in even vaccinated dogs were also reported. the clinical signs caused by different respiratory etiological agents are similar, which makes differential diagnosis imperative. an oligonucleotide microarray system was developed in this study. the wild type and vaccine strains of canine distemper virus (cdv), influenza virus, canine herp ... | 2017 | 28189583 |
| molecular surveillance of traditional and emerging pathogens associated with canine infectious respiratory disease. | a molecular survey for traditional and emerging pathogens associated with canine infectious respiratory disease (cird) was conducted in italy between 2011 and 2013 on a total of 138 dogs, including 78 early acute clinically ill cird animals, 22 non-clinical but exposed to clinically ill cird dogs and 38 cird convalescent dogs. the results showed that canine parainfluenza virus (cpiv) was the most commonly detected cird pathogen, followed by canine respiratory coronavirus (crcov), bordetella bron ... | 2016 | 27527760 |
| prevalence of canine infectious respiratory pathogens in asymptomatic dogs presented at us animal shelters. | to determine the prevalence of nine canine infectious respiratory disease (cird) pathogens in asymptomatic dogs presented at animal shelters across the united states. | 2015 | 26199194 |
| new and emerging pathogens in canine infectious respiratory disease. | canine infectious respiratory disease is a common, worldwide disease syndrome of multifactorial etiology. this review presents a summary of 6 viruses (canine respiratory coronavirus, canine pneumovirus, canine influenza virus, pantropic canine coronavirus, canine bocavirus, and canine hepacivirus) and 2 bacteria (streptococcus zooepidemicus and mycoplasma cynos) that have been associated with respiratory disease in dogs. for some pathogens a causal role is clear, whereas for others, ongoing rese ... | 2014 | 24232191 |
| transient cold agglutinins associated with mycoplasma cynos pneumonia in a dog. | this report details a case of reversible cold agglutinins in a dog with mycoplasma cynos pneumonia. an 11-month-old female spayed rhodesian ridgeback was presented for lethargy and cough. thoracic radiographs revealed an alveolar pattern present bilaterally in the cranioventral lung lobes. septic neutrophilic inflammation with suspected mycoplasma sp. organisms was noted on cytologic examination of a trans-tracheal wash, and the dog was treated empirically with iv ampicillin/sulbactam and enrofl ... | 2015 | 26356600 |
| comparison of the prevalence of mycoplasma species in dogs with and without respiratory disease. | aim of the study was to investigate the prevalence of mycoplasma species in dogs with and without signs of respiratory disease. bronchoalveolarlavage fluid (balf) and pharyngeal swabs were collected from 29 dogs with respiratory diseases (rd) and 16 dogs without signs of rd that were euthanised because of other diseases. samples were tested for mycoplasma species by pcr and culture, and sequencing was performed in mycoplasma species-positive balf samples. pharyngeal swabs were positive for mycop ... | 2015 | 26281443 |
| molecular characterisation of the mycoplasma cynos haemagglutinin hapa. | mycoplasma (m.) cynos is a proven pathogen of dogs causing respiratory infections including pneumonia. we examined 19 m. cynos strains isolated from different organs of dogs in austria, denmark and israel. all strains agglutinated mammalian and chicken erythrocytes. using erythrocytes of chickens or dogs as specific ligands we isolated an approximately 65 kda protein from cell-free supernatants of 3 m. cynos strains, which showed an apparent capacity for haemagglutination. the n-terminal sequenc ... | 2015 | 25465173 |
| complete genome sequence of mycoplasma cynos strain c142. | here we report the de novo genome sequencing of mycoplasma cynos strain c142, isolated from a dog with canine infectious respiratory disease (cird) in the united states. | 2013 | 23409256 |
| molecular identification of mycoplasma cynos from laboratory beagle dogs with respiratory disease. | in this study, we examined a colony of 20 beagle dogs in a laboratory animal facility. mycoplasma was detected by consensus pcr assay in 1 dog with respiratory and constitutional symptoms. none of the other dogs were affected. the dog was euthanized and necropsied. in postmortem examinations, gray or plum-colored gross lesions were found on the lung, most commonly in the apical and cardiac lobes. some lesions showed clear demarcation and consolidation. microscopic examination showed peribronchio ... | 2012 | 22474476 |
| h3n2 canine influenza virus infection in a dog. | in 2015, h3n2 canine influenza emerged in dogs in the greater chicago area. during this time, a 10-year-old german shepherd dog presented to the referring veterinarian with lethargy and coughing that quickly progressed to death. this report describes the macroscopic and microscopic lesions and the molecular testing performed to identify the novel north american h3n2 strain of canine influenza. the larynx, pharynx, and trachea were covered by a fibrinonecrotic membrane. bilaterally, the lungs had ... | 2017 | 28438112 |
| genome sequence of canine polyomavirus in respiratory secretions of dogs with pneumonia of unknown etiology. | we report here the first canine polyomavirus genome, identified by metagenomics in respiratory secretions of two dogs with severe pneumonia, which tested negative for all canine respiratory pathogens except mycoplasma cynos the isolate, canis familiaris polyomavirus 1 (dogpyv-1), is a beta polyomavirus whose closest known lt antigen relatives are primate polyomaviruses. | 2017 | 28729262 |
| comparison of five methods for isolation of dna from mycoplasma cynos. | extraction of dna from mycoplasma cultured on agar medium is difficult because the plasticity of these microorganisms enables agar penetration. this eventually causes cell loss during harvesting of colonies from the agar surface. here, we used the genelute™ gel extraction kit, which is usually used to purify polymerase chain reaction products, for extracting dna from mycoplasma. we compared the dna extraction efficiency of the genelute™ gel extraction kit from mycoplasma cynos cultured in agar m ... | 2017 | 28711528 |