| denaturing gradient gel electrophoresis analyses of the vertical distribution and diversity of vibrio spp. populations in the cariaco basin. | the cariaco system is the second largest permanently anoxic marine water body in the world. its water column is characterized by a pronounced vertical layering of microbial communities. the goal of our study was to investigate the vertical distribution and diversity of vibrio spp. present in the cariaco basin waters using denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s rdna fragments. representatives of the vibrio genus were detected by nested and direct pcr in seawater at 10 ... | 2011 | 21507026 |
| antibacterial compounds from marine vibrionaceae isolated on a global expedition. | on a global research expedition, over 500 bacterial strains inhibitory towards pathogenic bacteria were isolated. three hundred of the antibacterial strains were assigned to the vibrionaceae family. the purpose of the present study was to investigate the phylogeny and bioactivity of five vibrionaceae strains with pronounced antibacterial activity. these were identified as vibrio coralliilyticus (two strains), v. neptunius (two strains), and photobacterium halotolerans (one strain) on the basis o ... | 2010 | 21339958 |
| vibrio variabilis sp. nov. and vibrio marinus sp. nov., isolated from palythoa caribaeorum. | two novel vibrio isolates (r-40492t and r-40493t) originated from the zoanthid palythoa caribaeorum in brazil in 2005 were taxonomically characterized by means of a polyphasic approach, comprising multilocus sequence analysis (mlsa), dna-dna hybridizations (ddh), tm, and phenotypic characterization. phylogenetic analysis based on 16s rrna gene sequences showed that r-40492t and r-40493t fell within the genus vibrio, and were most closely related to each other with 99 % similarity. the similarity ... | 2011 | 21296931 |
| pathogenic bacteria isolated from disease outbreaks in shellfish hatcheries. first description of vibrio neptunius as an oyster pathogen. | shellfish hatcheries are often affected by disease outbreaks. three such episodes were investigated in different galician hatcheries in order to establish the relationship between present microbiota and mortalities. isolates were obtained from various parts of the hatcheries. experimental tests for pathogenicity were carried out in microscale experiments using selected strains on ostrea edulis larvae. the pathogenicity of 1 strain from each outbreak was demonstrated and shown to cause high morta ... | 2005 | 16408836 |
| use of reca as an alternative phylogenetic marker in the family vibrionaceae. | this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ... | 2004 | 15143042 |
| usefulness of a real time pcr platform for gc content and dna-dna hybridization similarity estimations in vibrios. | the aim of this study was to evaluate the utility of a real time pcr platform, to estimate the dna g+c mol% and the dna-dna hybridization (ddh) values in the genus vibrio. in total nine vibrio strains were used to determine the relationship between genomic dna g+c content and the tm (°c).the tm and hplc datasets fit a linear regression curve with a significant correlation coefficient, corroborating that the this methodology has a high correlation with the standard methodology based on hplc (r2 = ... | 2010 | 21057054 |
| vibrio neptunius sp. nov., vibrio brasiliensis sp. nov. and vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). | the fluorescent amplified fragment length polymorphism (faflp) groups a5 (21 isolates), a8 (6 isolates) and a23 (3 isolates) distinguished in an earlier paper (thompson et al., syst appl microbiol 24, 520-538, 2001) were examined in more depth. these three groups were phylogenetically related to vibrio tubiashii, but dna-dna hybridization experiments proved that the three aflp groups are in fact novel species. chemotaxonomic and phenotypic analyses further revealed several differences among the ... | 2003 | 12656180 |
| fast detection of vibrio species potentially pathogenic for mollusc. | vibrio tasmaniensis, vibrio splendidus and vibrio neptunius species were distributed worldwide and associated with aquaculture and have been reported as the cause of diseases in aquatic organisms. polyphasic analyses for bacterial identification are not feasible for routine diagnostic because of the time involved. the aim of this study is to design three pcr primer sets that can assist with fast detection of these species. they were designed from the 16s ribosomal rna gene, and pcr conditions we ... | 2009 | 19640660 |
| the dnaj gene as a novel phylogenetic marker for identification of vibrio species. | the utility of the dnaj gene for identifying vibrio species was investigated by analyzing dnaj sequences of 57 type strains and 22 clinical strains and comparing sequence homologies with those of the 16s rdna gene and other housekeeping genes (reca, rpoa, hsp60). among the 57 vibrio species, the mean sequence similarity of the dnaj gene (77.9%) was significantly less than that of the 16s rdna gene (97.2%), indicating a high discriminatory power of the dnaj gene. most vibrio species were, therefo ... | 2007 | 17207598 |