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use of insertional mutagenesis to tag putative parasitic fitness genes in the dutch elm disease fungus ophiostoma novo-ulmi subsp. novo-ulmi.we used insertional mutagenesis to produce genetically tagged mutants of the dutch elm disease fungus ophiostoma novo-ulmi subsp. novo-ulmi. we first optimized transformation of o. novo-ulmi protoplasts by the restriction enzyme mediated integration method. a concentration of 80 u of hindiii with 108 fungal protoplasts and 5 microg of plasmid dna was the most efficient for generating a high number of o. novo-ulmi mutants carrying a single insertion in their genome. mycelium- and yeast-like growt ...200818772944
identification of transcripts up-regulated in asexual and sexual fruiting bodies of the dutch elm disease pathogen ophiostoma novo-ulmi.suppression subtractive hybridization cdna libraries were prepared from asexual synnemata (s-lib) and sexual perithecia (p-lib) fruiting bodies of the dutch elm disease pathogen ophiostoma novo-ulmi subsp. novo-ulmi isolate h327 (mating-type mat1-1) consisting of 630 and 401 cdna clones, respectively. both libraries were differentially screened in duplicate with forward and reverse subtracted probes. up-regulated s-lib transcripts included those with homologies to phosphoenolpyruvate carboxykina ...201020725133
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