| [pseudomonas bacteriophage phikz--possible model for studying the genetic control of morphogenesis]. | a description of structure and some bilogical properties of a new virulent pseudomonas phage, phikz, is given in the paper. phage phikz has unique characteristics of particle (a large size of the head and the presence in the head of a structure which is visible on electron micrographs as crosswise striated substance). a cause of high-titer phage phikz formation on plates has been investigated. characteristics of single step growth experiments, sensitivity of free phage particles to heating, sds- ... | 1978 | 95987 |
| [certain physico-chemical properties of bacteriophage phikz]. | using uv-spectroscopy and circular dichroism (cd) phi kz bacteriophage and its dna were investigated. from the value of optical densities in the 320--400 nm range the size of the phi kz bacteriophage's head was determined; the diameter of phi kz bacteriophage head was found to be equal to 1300 +/- 100 a. phi kz bacteriophage dna has block structure and the gc-pair content is equal to 43.8 +/- 0.3%. phi kz bacteriophage cd spectrum has an unusual profile (in comparison with known bacteriophage cd ... | 1980 | 7421814 |
| electron microscopic study of dna packing in phi(kz) bacteriophage (pseudomonas aeruginosa) irradiated with laser in the presence of a dye. | dna packing of bacteriophage phikz (pseudomonas aeruginosa) was studied by electron microscopy after gentle destruction of the capsid with powerful laser impulse in aqueous virus suspension in the presence organic dye. suspension of destroyed phage heads contained complexes of genomic dna with endogenous capsid proteins characterized by pronounced regular structure. the type of these structures corresponds to one the main model of phage dna packing, the so-called coil packing. | 2001 | 11719926 |
| atomic force microscopy analysis of bacteriophages phikz and t4. | bacteriophage phikz was investigated by atomic force microscopy (afm) and transmission electron microscopy (tem). the well-known phage t4 was used as a reference sample. reproducible contact mode afm images of native and partially disintegrated particles of bacteriophage phikz in air were obtained. it was demonstrated that the heads of phikz and t4 phages were compressed differently and depended on adsorption onto highly ordered pyrolytic graphite and mica. we have established a procedure to par ... | 2001 | 11794617 |
| the genome of bacteriophage phikz of pseudomonas aeruginosa. | bacteriophage phikz is a giant virus that efficiently infects pseudomonas aeruginosa strains pathogenic to human and, therefore, it is attractive for phage therapy. we present here the complete phikz genome sequence and a preliminary analysis of its genome structure. the 280,334 bp genome is a linear, circularly permutated and terminally redundant, a+t-rich double-stranded dna molecule. the phikz dna has no detectable sequence homology to other viruses and microorganisms, and it does not contain ... | 2002 | 11916376 |
| myoviridae bacteriophages of pseudomonas aeruginosa: a long and complex evolutionary pathway. | recently we have accomplished the entire dna sequence of bacteriophage phikz, a giant virus infecting pseudomonas aeruginosa. the 280334-bp of phikz genome is a linear, circularly permutated and terminally redundant, at-rich dsdna molecule that contains no sites for noti, psti, saci, smai, xhoi and xmaiii endonucleases. limited homology to other bacteriophages on the dna and protein levels indicated that phikz represents a distinct branch of the myoviridae family. in this work, we analyzed a gro ... | 2003 | 12798231 |
| a three-dimensional cryo-electron microscopy structure of the bacteriophage phikz head. | the three-dimensional structure of the pseudomonas aeruginosa bacteriophage phikz head has been determined by cryo-electron microscopy and image reconstruction to 18a resolution. the head has icosahedral symmetry measuring 1455 a in diameter along 5-fold axes and a unique portal vertex to which is attached an approximately 1800 a-long contractile tail. the 65 kda major capsid protein, gp120, is organized into a surface lattice of hexamers, with t = 27 triangulation. the shape and size of the hex ... | 2005 | 16081102 |
| factors influencing the synonymous codon and amino acid usage bias in at-rich pseudomonas aeruginosa phage phikz. | to reveal how the at-rich genome of bacteriophage phikz has been shaped in order to carry out its growth in the gc-rich host pseudomonas aeruginosa, synonymous codon and amino acid usage bias of phikz was investigated and the data were compared with that of p. aeruginosa. it was found that synonymous codon and amino acid usage of phikz was distinct from that of p. aeruginosa. in contrast to p. aeruginosa, the third codon position of the synonymous codons of phikz carries mostly a or t base; codo ... | 2005 | 16143818 |
| properties of the endolytic transglycosylase encoded by gene 144 of pseudomonas aeruginosa bacteriophage phikz. | bacteriophage endolysins degrading bacterial cell walls are prospective enzymes for therapy of bacterial infections. the genome of the giant bacteriophage phikz of pseudomonas aeruginosa encodes two endolysins, gene products (g.p.) 144 and 181, which are homologous to lytic transglycosylases. gene 144 encoding a 260 amino acid residue protein was cloned into the plasmid expression vector. recombinant g.p. 144 purified from escherichia coli effectively degrades chloroform-treated p. aeruginosa ce ... | 2006 | 16545067 |
| cryo-em study of the pseudomonas bacteriophage phikz. | the phikz virus is one of the largest known bacteriophages. it infects pseudomonas aeruginosa, which is frequently pathogenic in humans, and, therefore, has potential for phage therapy. the phikz virion consists of an approximately 1450 a diameter icosahedral head and an approximately 2000 a long contractile tail. the structure of the phikz tail has been determined using cryo-electron microscopy. the phikz tail is much longer than that of bacteriophage t4. however, the helical parameters of thei ... | 2007 | 17850749 |
| the structural peptidoglycan hydrolase gp181 of bacteriophage phikz. | gp181 (2237 amino acids) of pseudomonas aeruginosa bacteriophage phikz (myoviridae) is a structural virion protein, which bears a peptidoglycan hydrolase domain near its c-terminus. this protein is supposed to degrade the peptidoglycan locally during the infection process. nine deletional mutants allowed delineation of the peptidoglycan hydrolase domain between amino acids 1880-2042 (gp181m8) and analysis of its biochemical properties. gp181m8 tolerates a high ionic strength (>320mm) and is less ... | 2008 | 18671939 |
| characterization of tail sheath protein of giant bacteriophage phikz pseudomonas aeruginosa. | the tail sheath protein of giant bacteriophage phikz pseudomonas aeruginosa encoded by gene 29 was identified and its expression system was developed. localization of the protein on the virion was confirmed by immunoelectron microscopy. properties of gene product (gp) 29 were studied by electron microscopy, immunoblotting and limited trypsinolysis. recombinant gp29 assembles into the regular tubular structures (polysheaths) of variable length. trypsin digestion of gp29 within polysheaths or exte ... | 2009 | 19822340 |
| structural conservation of the myoviridae phage tail sheath protein fold. | bacteriophage phikz is a giant phage that infects pseudomonas aeruginosa, a human pathogen. the phikz virion consists of a 1450 å diameter icosahedral head and a 2000 å-long contractile tail. the structure of the whole virus was previously reported, showing that its tail organization in the extended state is similar to the well-studied myovirus bacteriophage t4 tail. the crystal structure of a tail sheath protein fragment of phikz was determined to 2.4 å resolution. furthermore, crystal structur ... | 2011 | 22153511 |
| complete genome sequence of erwinia amylovora bacteriophage vb_eamm_ea35-70. | the complete genome of an erwinia amylovora bacteriophage, vb_eamm_ea35-70 (ea35-70), is 271,084 bp, encodes 318 putative proteins, and contains one trna. comparative analysis with other myoviridae genomes suggests that ea35-70 is related to the phikzlikevirus genus within the family myoviridae, since 26% of ea35-70 proteins share homology to proteins in pseudomonas phage φkz. | 2014 | 25146132 |