| fungal degradation of aromatic nitriles. enzymology of c-n cleavage by fusarium solani. | 1. a strain of the fungus fusarium solani able to use benzonitrile as sole source of carbon and nitrogen was isolated by elective culture. 2. respiration studies indicate that the nitrile, after degradation to benzoate, is catabolized via catechol or alternatively via p-hydroxybenzoate and 3,4-dihydroxybenzoate. 3. cell-free extracts of benzonitrile-grown cells contain an enzyme mediating the conversion of benzonitrile into benzoate and ammonia. 4. the nitrilase enzyme was purified by deae-cellu ... | 1977 | 23761 |
| studies on a strain of fusarium solani (mart.) sacc. isolated from a case of mycotic keratitis. | a strain of fusarium solani (mart.) sacc. (imi-216517), isolated from a patient of mycotic keratitis, produced experimental keratomycosis in albino rabbit cornea and survived in internal tissues of albino mice for varying periods. alantolactone, isolated from the plant - inula racemosa hook. f. exhibited marked in vitro fungistatic activity against this strain of f. solani at 100-200 microgram/ml concentrations. the strain was less sensitive to amphotericin b and showed more acid than alkaline p ... | 1979 | 226886 |
| new antibiotic pigments related to fusarubin from fusarium solani (mart.) sacc. i. fermentation, isolation, and antimicrobial activities. | a cholesterol-decomposing fungus, fusarium solani (mart.) sacc. strain pp 96, was found to produce several different naphthaquinone pigments in a glycerol-mineral salts medium. three novel compounds structurally related to fusarubin were isolated by chloroform extration followed by silicic acid column chromatography and preparative thin-layer chromatography. the purified compounds were found to have relatively low activity against bacteria, yeasts and filamentous fungi. | 1979 | 541262 |
| new antibiotic pigments related to fusarubin from fusarium solani (mart.) sacc. ii. structure elucidations. | three antibiotic pigments isolated from fusarium solani and related to fusarubin (4) were shown to be o-ethylfusarubin (2), hydroxidihydrofusarubin (3) and o-ethylhydroxydihydrofusarubin (1). | 1979 | 541263 |
| [stabilization of dextranase from penicillium funiculosum and fusarium solani during heating and freeze-drying]. | freeze-drying of highly purified dextranse from penicillium funiculosum and fusarium solani was accompanied by 90% losses of enzyme activity and solubility. many carbohydrates were tested as stabilizers, e.g. glucose, maltose, lactose, polyglucine, dextranase hydrolyzate of polyglucine as well as mannitol and ammonium sulfate. polyglucine, its hydrolyzate, and glucose proved most effective stabilizers. the stabilizing effect of polyglucine hydrolyzate of dextranase during its heating and freeze- ... | 1979 | 538015 |
| direct evidence for the presence of beta-hydroxyphenylalanine and beta-hydroxytyrosine in cutinase from fusarium solani pisi. | | 1979 | 507809 |
| induction of a biopolyester hydrolase (cutinase) by low levels of cutin monomers in fusarium solani f.sp. pisi. | cutin hydrolysate induced the production of an extracellular cutinase by glucose-grown fusarium solani f. sp. pisi. the rate of production depended on the amount of cutin hydrolysate added up to 80 mug/ml, and saturation was attained at this level. glucose was found to be a repressor of cutinase production. a radial immunodiffusion assay for cutinase was developed, and the induction of cutinase by cutin hydrolysate was confirmed by this direct assay. when cutinase was induced by cutin hydrolysat ... | 1978 | 415052 |
| suitability of membrane-filter techniques to study the ultrastructure of fusarium solani in soil. | the suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of fusarium solani from soil was tested. an improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical observations is described. best results were obtained if macroconidia were incubated in soil between two nucleopore membrane filters. both chlamydospore morphology and lysis, howev ... | 1976 | 974910 |
| ultrastructure of forming and dormant chlamydospores of fusarium solani in soil. | chlamydospores of fusarium solani f. sp. cucurbitae, f. solani f. sp. phaseoli and f. solani f. sp. pisi formed in soil were recovered at intervals and examined by electron microscopy. cell wall material outside the newly formed chlamydospore cell wall gradually disintegrated. protoplasts of chlamydospore cells lyse without prior penetration of the cell wall by microorganisms. the mechanisms of lysis of chlamydospores of f. solani in soil are discussed. | 1976 | 974911 |
| cellulase from fusarium solani: purification and properties of the c1 component. | the c1 component from fusarium solani cellulase was purified extensively by molecular-sieve chromatography on ultrogel aca-54 and ion-exchange chromatography on deae-sephadex. the purified component showed little capacity for hydrolysing highly ordered substrates (e.g., cotton fibre), but poorly ordered substrates (e.g., h3po4-swollen cellulose), and the soluble cello-oligosaccharides cellotetraose and cellohexaose, were readily hydrolysed; cellobiose was the principal product in each case. atta ... | 1977 | 409496 |
| detection of mutagens produced by fungi with the salmonella typhimurium assay. | forty-one fungal isolates (one isolate per species) representing common plant pathogens and food crop contaminants were grown on sterile, polished rice and assayed for mutagenic activity in the salmonella typhimurium-microsome system. initially, single doses of aqueous and chloroform extracts of the moldy rice were assayed against the ta100 tester strain by incorporating extracts into the growth medium and by applying small quantities on disks placed on the agar surface. suspected activity was e ... | 1978 | 354528 |
| fusarium solani endophthalmitis without primary corneal involvement. | a 45-year-old woman developed a fungal endophthalmitis caused by fusarium solani. no primary corneal lesion existed. therefore, the focus of infection was considered endogenous. although disseminated systemic infections with fusarium species have been described in compromised hosts, including a patient with metastatic panophthalmitis, we believe this is the first report of an endogenous endophthalmitis caused by f. solani in a healthy individual. | 1979 | 315715 |
| an animal model of fusarium solani endophthalmitis. | infection with fusarium solani was established by injecting spores into the anterior chambers of outbred wistar rats. the disease produced was remarkably similar to that reported in man and progressed to a fulminating endophthalmitis. attenuation of the disease could be accomplished by repeated animal passage of the isolate. | 1979 | 312111 |
| in vitro antibiotic synergism against ocular fungal isolates. | a microtiter method for the determination of fungal sensitivities was used to determine the minimal inhibitory and fungicidal concentrations of two antifungal agents, amphotericin b and natamycin, both alone and in combination with four different antibiotics: rifampin, gentamicin, clindamycin, and tetracycline. synergism was defined as a fourfold or greater reduction in the minimal inhibitory concentration, minimal fungicidal concentration, or both, of the antifungal agent in the presence of ant ... | 1978 | 309728 |
| fate of naturally occurring epoxy acids: a soluble epoxide hydrase, which catalyzes cis hydration, from fusarium solani pisi. | | 1975 | 235241 |
| effect of three nematicides on the growth of some phytopathogenic bacteria and fungi. | the effect of three nematicides, aldicarb, fensulfothion, and phenamiphos at four concentrations (1, 5, 25, and 125 ppm) was tested on the growth of five bacteria, agrobacterium tumefaciens, corynebacterium fascians, erwinia carotovora, pseudomonas solanacearum, and streptomyces scabies and four fungi, fusarium oxysporum f. sp. vasinfectum, fusarium solani, rhizoctonia solani, and sclerotium bataticola. of the bacteria, p. solanacearum was most affected by the chemicals at all concentrations, wh ... | 1977 | 143857 |
| roles of low ph, carbon and inorganic nitrogen source use in chlamydospore formation by fusarium solani. | citrate and malate were poorer sources of exogenous carbon than several hexose, pentose, or disaccharide sugars for supporting macroconidial germination by fusarium solani at high conidial density (1 x 10(5) condia/ml). only citrate, however, failed to block chlamydospore morphogenesis to a degree comparable to glucose or other readily used sugars. mostly immature chlamydospores were formed in the presence of citrate. at low conidial density (5 x 10(3) conidia/ml), exogenous carbon-independent m ... | 1976 | 10071 |
| characterization of a monoclonal antibody (p40) against the 68 kd major allergen of penicillium notatum. | a monoclonal antibody (moab p40) against the 68 kd major allergen of penicillium notatum (p. notatum) was obtained by immunizing the mouse with a crude extract of p. notatum. analysed by two-dimensional gel electrophoresis and immunoblotting, p40 reacted with two different isoforms of the 68 kd component of p. notatum with pis of 5.4 and 5.5. in addition to p. notatum, p40 showed positive elisa activity to aspergillus fumigatus (a. fumigatus) but not to components of six other fungi including al ... | 1992 | 1377093 |
| soil fungistasis: elevation of the exogenous carbon and nitrogen requirements for spore germination by fungistatic volatiles in soils. | axenic, washed conidia of fusarium solani f. sp. phaseoli, aspergillus flavus, and verticillium albo-atrum were placed on washed difco purified agar discs along with an inorganic salt solution containing various levels of carbon and nitrogen substrates. these discs were exposed to volatiles from six soils (ph 5.1-8.6). fusarium solani macroconidial germination was inhibited mostly by volatiles from soils of ph 5.1, 6.1, 7.0, and 7.5, but high levels of glucose and nh4cl reversed this inhibition, ... | 1975 | 135 |
| microbiological transformations of steroids. iv. factors affecting the transformation of progesterone by non-germinating spores of fusarium solani. | | 1977 | 854545 |
| animal model of fusarium solani keratitis. | because of the frequency of fungal keratitis due to fusarium solani, we needed a sustained, progressive infection in an animal model to determine the mechanisms of pathogenicity and to evaluate the new antifungal agents. pigmented rabbits interlamellarly injected with actively germinating conidia from lyophilized temperature-tolerant strains of f. solani produced sustained culture-positive ulcers in high percentage of eyes at two and three weeks, pretreatment with subconjunctival corticosteroids ... | 1975 | 804817 |
| mycotic keratitis in nigeria. a study of 21 cases. | a clinical and mycological study of 21 cases of mycotic keratitis, a clinical entity not yet reported from nigeria or west africa, showed that fusarium solani was the predominant aetiological agent. it was isolated from 12 cases. four of the remaining nine cases were caused by aspergillus fumigatus, one by a. flavus, two by penicillium citrinum, and one each by p. expansum and penicillium sp. all the 12 isolates of f. solani grew well at 37 degrees c and survived at 40 degrees c. two cases, one ... | 1976 | 791355 |
| lipid body content and persistence of chlamydospores of fusarium solani in soil. | chlamydospores of fusarium solani f. sp. pisi. low or high in lipid content, were produced in soil from "low and high reserve" macroconidia, respectively. the development of "low and high reserve" populations of macroconidia and chlamydospores in soil was studied. the rate of conversion of macroconidia into chlamydospores is influenced by the lipid content of the macroconidia. the long-term persistence of chlamydospores in soil seems to be independent of the lipid content of the chlamydospore ce ... | 1978 | 754878 |
| [post-traumatic osteomyelitis caused by fusarium solani]. | | 1992 | 1489787 |
| role of opportunistic fungi in ocular infections in nigeria. | the importance of opportunistic fungal pathogens in causing ocular infections is emphasized. a study was conducted over a period of 4 years (1974--1977) to investigate the role of opportunistic fungi in causing mycotic keratitis and to elucidate certain aspects of epidemiology of this disease in nigeria. fifty-nine cases of corneal ulcers of suspected mycotic etiology were investigated. fungal etiology was confirmed in 42 of these cases. the predominant etiological agent was fusarium solani in 1 ... | 1978 | 745623 |
| toxicity of zinc to fungi, bacteria, and coliphages: influence of chloride ions. | a 10 mm concentration of zn2+ decreased the survival of escherichia coli; enhanced the survival of bacillus cereus; did not significantly affect the survival of pseudomonas aeruginosa, norcardia corallina, and t1, t7, p1, and phi80 coliphages; completely inhibited mycelial growth of rhizoctonia solani; and reduced mycelial growth of fusarium solani, cunninghamella echinulata, aspergillus niger, and trichoderma viride. the toxicity of zinc to the fungi, bacteria, and coliphages was unaffected, le ... | 1978 | 736544 |
| effect of fusarium isolates and their filtrates on respiratory rate and chemical analysis of squash plants. | the highly pathogenic isolate stimulated the emergence of the squash seedlings first, caused, however, the highest death rate of the seedlings finally. fusarium isolates and their culture filtrates inhibited the respiratory rate of squash plants significantly. however, f. oxysporum isolates inhibited respiration more than f. solani isolates. seasonal changes of respiration decline show that the respiratory rate decreased with plant growth in the case of infested soil and of plants injected with ... | 1978 | 685535 |
| effects of 4-ipomeanol, a product from mold-damaged sweet potatoes, on the bovine lung. | cattle given intraruminal administration of 4-ipomeanol, a furanoterpenoid originally obtained from sweet potatoes infected with fusarium solani (f. javanicum), developed a respiratory syndrome clinically and histologically indistinguishable from atypical interstitial pneumonia. there were edema and emphysema in the lungs and mediastinum. the maximum nonlethal oral dose of 4-ipomeanol was estimated to be between 7.5 and 9 mg/kg of body weight. | 1978 | 685084 |
| lack of activity of amphotericin b in systemic murine fusarial infection. | systemic fusarial infections have emerged as a significant cause of mortality in cancer patients. yet, little is known about the management of these infections. the in vivo antifungal activity of amphotericin b in cf1 mice with disseminated fusarial infections was studied. two pathogenic strains of fusarium solani were used. intraperitoneal administration of amphotericin b in daily doses of 0.5, 1, and 2 mg/kg for less than or equal to 10 days did not prolong survival of treated animals. clearan ... | 1992 | 1583339 |
| the metabolism of chlorogenic acid in tuber discs of a resistant and a susceptible potato cultivar after inoculation with fusarium solani var. caeruleum [proceedings]. | | 1978 | 648730 |
| the source of androgenic activity in the african wood funtumia latifolia: a steroid hormone formed by the action of fusarium solani. | | 1978 | 569228 |
| fusarium wilt of prunus armeniaca seedlings. | fusarium solani (mart.) sacc. was found to be the causal pathogen of fusarium wilt of prunus armeniaca seedlings. the fungus pathogenicity could be correlated with the increase in its mycelial growth and conidial germination under the influence of the host root exudates, volatile and gaseous exudates of either germinating seeds or roots, and the content of the host seedlings. chromatographic and biological detection for indol derivatives in host root exudates indicated the presence of beta-indol ... | 1977 | 878711 |
| isolation of ioxynil degraders from soil-enrichment cultures. | a soil enrichment technique was used to isolate microorganisms which could degrade ioxynil (3,5-diiodo-4-hydroxybenzonitrile). many isolates obtained were able to degrade ioxynil to various products. however, only a fungal isolate (fusarium solani) and a gram-negative bacterium (klebsiella ozaenae) released 14co2 from ring-labeled ioxynil. no appreciable degradation was detected in pure cultures without the addition of exogenous nutrients. results indicated that the degradation of ioxynil to co2 ... | 1976 | 944081 |
| degradation of lignocellulosic material and humus formation by fungi. | cellulase activity and degradation of cellulose and lignin in wheat straw and formation of humus during degradation by aspergillus sp., chaetomium globosum, fusarium solani, paecilomyces varioti, penicillium chrysogenum and trichoderma viride qm9414 were studied. c. globosum, f. solani, p. varioti and p. chrysogenum produced little or no cellulase when grown on cellulose powder. however, all produced cellulase (filter paper degrading activity) when grown on wheat straw. all the cultures degraded ... | 1979 | 507620 |
| production of trichothecene mycotoxins by fusarium species in shake culture. | twelve t-2 toxin-producing isolates and four fusarenon-x-producing isolates of fusarium species were examined for their ability to produce trichothecene mycotoxins in shake culture and jar fermentation. t-2 toxin producers such as fusarium solani, f. sporotrichiodes, and f. tricinctum produced t-2 toxin and neosolaniol in semisynthetic medium. f. solani m-1-1 produced the largest amount of the mycotoxins in a nutrient medium consisting of 5% glucose (or sucrose), 0.1% peptone, and 0.1% yeast ext ... | 1975 | 1147619 |
| growth responses of two phytopathogenic fungi to fernasan in culture media. | the toxic effect of fernasan (containing 25% thiram) was tested on rhizoctonia solani and fusarium solani in liquid and agar media. the fungicide was more effective in liquid (at 100 p.p.m.) than in solid media, where 400 p.p.m. checked the growth of r. solani, but was ineffective against f. solani. both fungi exhibited a zone of inversion growth in liquid culture whereby certain intermediate concentrations were less inhibitory than lower or subsequently higher doses. similar reaction was record ... | 1975 | 1152969 |
| hydrolysis of plant cuticle by plant pathogens. purification, amino acid composition, and molecular weight of two isozymes of cutinase and a nonspecific esterase from fusarium solani f. pisi. | the extracellular fluid of the plant pathogen, fusarium solani f. pisi, grown on the plant cuticular polymer, cutin, was shown to contain cutinase and p-nitrophenyl palmitate hydrolase activities (r.e. purdy and p.e. kolattukudy (1973), arch. biochem. biophys. 159, 61). from this extracellular fluid two isozymes of cutinase and a nonspecific esterase (p-nitrophenyl palmitate hydrolase) were isolated using sephedex g-100 gel filtration, qae-sephadex chromatography, and se-sephedex chromatography. ... | 1975 | 1156575 |
| in vitro effect of some antibiotics on the growth and respiration of fusarium solani f. pisi. | | 1975 | 1236090 |
| the diagnosis and management of keratomycoses. i. cause and diagnosis. | causative isolates, clinical features, and laboratory studies are reported for sixty-one cases of culture-proved mycotic keratitis. isolates are categorized into four groups, including 36 fusarium solani, 11 other moniliaceae species, seven dematiaceae, and seven yeasts. of the 61 patients, 42 were men. mild outdoor trauma was sustained in 14 of 24 cases. patients were often referred with a clinical diagnosis of presumed fungal keratitis, within one week of symptom development, and usually had n ... | 1975 | 1237283 |
| chemical structures of main extracellular polysaccharides of alternaria solani and fusarium solani. studies on fungal polysaccharides. xviii. | | 1975 | 1237359 |
| exogenous mycotic infections of the eye and adnexia. | three cases of exogenous mycotic infections of the eye are presented. the first is a case of keratitis in a patient suffering from glaucoma simplex who removed a foreign body from the cornea, caused by fusarium solani, generally known as a saprophytic soil and plant inhabitant. as factors predisposing the patient to infection, trauma to the cornea like injury by a foreign body, as well as the preexisting disease of the eye, are discussed. the other case illustrates the change in the pathogenicit ... | 1978 | 308789 |
| hydrolysis of plant cuticle by plant pathogens. properties of cutinase i, cutinase ii, and a nonspecific esterase isolated from fusarium solani pisi. | the properties of the homogeneous cutinase i, cutinase ii, and the nonspecific esterase isolated from the extracellular fluid of cutin-grown fusarium solani f. pisi (r.e. purdy and p.e. kolattukudy (1975), biochemistry, preceding paper in this issue) were investigated. using tritiated apple cutin as substrate, the two cutinases showed similar substrate concentration dependence, protein concentration dependence, time course profiles, and ph dependence profiles with optimum near 10.0. using unlabe ... | 1975 | 239740 |
| induction and purification of kievitone hydratase from fusarium solani f. sp. phaseoli. | fusarium solani f. sp. phaseoli is capable of detoxifying the major isoflavonoid phytoalexins produced by its host plant phaseolus vulgaris. one of the enzymic activities involved is kievitone hydratase (khase), a secreted glycoprotein which catalyses the conversion of kievitone to the less fungitoxic derivative, kievitone hydrate. even under conditions of substrate induction, the enzyme is expressed at levels that are too low for satisfactory purification. therefore, several other isoflavonoids ... | 1990 | 1366757 |
| construction of a 7-aminocephalosporanic acid (7aca) biosynthetic operon and direct production of 7aca in acremonium chrysogenum. | we have used cdna encoding d-amino acid oxidase, and genomic dna encoding cephalosporin acylase from fusarium solani and pseudomonas diminuta, respectively, to construct a novel hybrid 7-aminocephalosporanic acid (7aca) biosynthetic operon under the control of regulatory elements from the alkaline protease gene of acremonium chrysogenum. transformants of a. chrysogenum bc2116, a high cephalosporin-producing strain, containing this operon, synthesized and secreted low levels of 7aca. although the ... | 1991 | 1369453 |
| cross-reactivity among antigens of different air-borne fungi detected by elisa using five monoclonal antibodies against penicillium notatum. | cross-reactivity among antigens of 12 genera of air-borne fungi, 13 species of penicillium, and 5 species of aspergillus was studied by elisa using five monoclonal antibodies (moabs) against penicillium notatum. epitopes recognized by all the five moabs were susceptible to treatment of mild periodate oxidation and may therefore be associated with carbohydrates. furthermore, our results showed that there is cross-reactivity among antigens of penicillium, aspergillus, and eurotium species. by usin ... | 1990 | 2178052 |
| maxillary sinus fusariosis in immunocompetent hosts. | we report the first known cases of fusariosis of maxillary sinus with granuloma and oro-antral fistula in two immunocompetent hosts. fusarium solani was demonstrated in the direct microscopic examination and isolated in heavy growth from the biopsy materials. both these patients were successfully treated with oral ketoconazole (200 mg daily) for three weeks followed by a caldwell-luc operation. ketoconazole was continued for two months post-operatively. | 1992 | 1402369 |
| cutinase in cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded rnas. | plant-pathogenic fungi produce cutinase, an enzyme required to degrade plant cuticles and facilitate penetration into the host. the absence of cutinase or a decrease in its production has been associated with a decrease in pathogenicity of the fungus. a set of isogenic strains of cryphonectria parasitica, the chestnut blight fungus, was tested for the presence and amounts of cutinase activity. the virulent strain of c. parasitica produced and secreted significantly higher amounts of cutinase tha ... | 1992 | 1406643 |
| molecular characterization of a pea beta-1,3-glucanase induced by fusarium solani and chitosan challenge. | beta-glucanases are prominent proteins in pea endocarp tissue responding to fungal infection. we have cloned and sequenced a partial pea cdna clone, ppig312, corresponding to a beta-1,3-glucanase in pea pods challenged with the incompatible pathogen fusarium solani f. sp. phaseoli. the insert from the partial pea cdna was used to probe a genomic library derived from pea leaves of the same cultivar. one of the genomic clones, ppig4-3, contained the complete coding sequence for a mature beta-1,3-g ... | 1992 | 1450378 |
| antimicrobial activity of naphthoquinones from fusaria. | twenty-two naphthoquinone compounds isolated or derived synthetically from culture extracts of fusarium solani and f. oxysporum were examined for antimicrobial activity. fifteen exhibited antibiotic activity against staphylococcus aureus, and 12 were active against streptococcus pyogenes, but none were active at the highest rate of 128 micrograms/ml against escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, serratia marcescens, or pseudomonas aeruginosa. of 8 plant path ... | 1990 | 2233983 |
| an extracellular enzyme from fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin. | among the antimicrobial phytoalexins produced by phaseolus vulgaris (french bean) are the prenylated isoflavonoids kievitone and phaseollidin. two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. the enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electroph ... | 1992 | 1521768 |
| [fusarium solani fusariosis in a leukemic adolescent]. | | 1992 | 1530253 |
| onychomycosis as a possible origin of disseminated fusarium solani infection in a patient with severe aplastic anemia. | | 1992 | 1534694 |
| fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent. | lipases belong to a class of esterases whose activity on triglycerides is greatly enhanced at lipid-water interfaces. this phenomenon, called interfacial activation, has a structural explanation: a hydrophobic lid, which at rest covers the catalytic site, is displaced on substrate or inhibitor binding and probably interacts with the lipid matrix. fusarium solani pisi cutinase belongs to a group of homologous enzymes of relative molecular mass 22-25k (ref. 7) capable of degrading cutin, the insol ... | 1992 | 1560844 |
| a case of contact lens related fusarium solani keratitis. | | 1992 | 1606675 |
| high efficiency transformation of fusarium solani f. sp. cucurbitae race 2 (mating population v). | a cosmid vector, suitable for library construction and dna transformation in filamentous fungi, has been constructed and a reliable and highly efficient peg-mediated dna transformation system for f. solani f. sp. cucurbitae, based on resistance to hygromycin b, has been developed for use with this vector. this transformation system yielded 10(4) transformants per micrograms of dna when using 10(7) protoplasts. factors important in achieving high efficiency included: the maintenance of an osmotic ... | 1992 | 1617735 |
| invasion and survival of fusarium solani in the dexamethasone-treated cornea of rabbits. | electron microscopic observations, as well as in vitro experiments, on experimental fusarium solani keratitis of rabbits were performed to study the mode of fungal invasion into the corneal stroma, the interactions between f. solani and inflammatory cells under the influence of topical dexamethasone (dxm) treatment, and the survival mechanism of the fungi in the dxm-treated cornea. electron microscopy showed that, while the fungus invaded into the corneal stroma, digestion of collagen fibrils oc ... | 1991 | 1667672 |
| the fusarium solani-induced expression of a pea gene family encoding high cysteine content proteins. | two pea genes, pi39 and pi230, which are specifically induced by two forma specials of fusarium solani, encode closely related proteins with predicted molecular masses (mr) of 8.2 and 8 kda, respectively. both proteins contain a signal sequence and are cleaved to mature proteins of mr 5 kda as indicated by an in vitro translation system. the mature proteins contain about 17% cysteine residues and have the potential to form four disulfide bonds. the two proteins share extensive homology in their ... | 1991 | 1799696 |
| differentiation of fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic dna. | we have used a pcr-based technique, involving the random amplification of polymorphic dna (rapd), to assess genome variability between 21 isolates from f. solani f. sp. cucurbitae races 1 and 2. based on rapd marker patterns the isolates fell into two distinct groups corresponding to mating populations mpi and mpv. four isolates that could not be assigned to one or other mating population by traditional means were distinguished by rapd patterns. seven polymorphic rapd products were used to probe ... | 1991 | 1807830 |
| natural occurrence of zearalenone and toxicogenic fungi in amaranth grain. | zearalenone was detected as the natural contaminant in two samples of amaranthus cruentus grains (1980 micrograms/kg and 420 micrograms/kg, respectively). fungi isolated from these samples were screened for mycotoxin production. two of eight isolates of fusarium (f. equiseti and f. moniliforme) produced zearalenone. one of four isolates of aspergillus flavus and all four isolates of a. parasiticus produced aflatoxins. other species potentially toxicogenic such as aspergillus versicolor, penicill ... | 1991 | 1830753 |
| identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor. | plant cutin monomers trigger, and glucose suppresses, the expression of the cutinase gene of pathogenic fungi. to identify the cutinase promoter region responsible for induction by the unique plant components, a promoter analysis was done with transformants. plasmids were constructed that contained (i) the 5' flanking region of the cutinase gene or its deletion mutants from fusarium solani pisi fused with a chloramphenicol acetyltransferase (cat) reporter gene and (ii) a constitutive promoter fu ... | 1991 | 1896470 |
| experimental fusarial hyalohyphomycosis in a murine model. | the pathogenicity of two clinical strains of fusarium solani was studied in normal and transiently neutropenic outbred cf1 and cd1 male mice. three inocula (5 x 10(5), 1 x 10(6), and 5 x 10(6) spores/animal) were tested. groups of 10 mice each were injected with a single intravenous dose of one inoculum. mortality correlated with the dose of inoculum, as survival was significantly shorter in mice injected with 5 x 10(6) cfu/mouse than in mice that received 1 x 10(6) or 5 x 10(5) cfu/mouse (p les ... | 1991 | 1940473 |
| squamous cell carcinoma arising in long-standing granulomatous hyalohyphomycosis caused by fusarium solani. | | 1991 | 1952991 |
| structure and expression of cdna for d-amino acid oxidase active against cephalosporin c from fusarium solani. | d-amino acid oxidase (dao) was extracted and purified from cultured mycelia of fusarium solani m-0718 (ferm p-2688). the enzyme was able to oxidatively deaminate cephalosporin c to 7-beta-(5-carboxy-5-oxopentanamido)cephalosporanic acid. ninety-eight amino acid residues of the f. solani dao were determined by sequence analysis of 9 peptides derived from acromobacter protease i digests of the protein. complementary dnas encoding f. solani dao were isolated from the f. solani cdna library by hybri ... | 1990 | 1982443 |
| fungi of virgin and cultivated soil of salhiah desert, egypt. | 27 species and 13 genera of fungi were identified from virgin and cultivated soil of salhiah. the most abundant species of phosphate solubilizing fungi were aspergillus nidulans, a. niger, a flavus, penicillium lilacinum, p. frequentans and fusarium moniliforme. on cellulose agar the most prevalent species were chaetomium bostrychodes, c. olivaceum, humicola fuscoatra, aspergillus flavus, a. nidulans, a. niger, a. ochraceus, fusarium solani and f. oxysporum. on xylan agar aspergillus fumigatus, ... | 1990 | 2077791 |
| [fusarium solani bronchopneumonia in a patient with acute myeloblastic leukemia]. | | 1990 | 2098140 |
| diagnosis and successful treatment of fusariosis in the compromised host. | we present six cases of fusariosis caused by fusarium solani that were diagnosed over a three-year period in 166 adult patients undergoing chemotherapy for acute leukemia. necrotic skin lesions were evident in four patients, fungemia in three, and a deep cellulitis around a great toe nail at the time of a febrile illness in two. the mean minimal inhibitory concentration (mic) of amphotericin b was 3.3 micrograms/ml and of miconazole, 5.3 micrograms/ml; all isolates were resistant to 5-fluorocyto ... | 1988 | 3053920 |
| disseminated fusarium solani infection with cutaneous nodules in a bone marrow transplant patient. | fusarium is a ubiquitous fungus that commonly colonizes ulcerated, burned, or traumatized skin and may cause keratitis and onychomycosis in healthy hosts. serious disseminated infection due to fusarium has been reported with increasing frequency in immunocompromised patients. we describe a bone marrow transplant patient who developed fungal septicemia and disseminated skin nodules due to fusarium solani. fusarium should be recognized as a potential cause of deep fungal infection in immunocomprom ... | 1988 | 3069758 |
| cloning and characterization of a disease resistance response gene in pea inducible by fusarium solani. | disease resistance response genes (drrg) of peas are expressed as the tissue is expressing race-specific or nonhost resistance. a pea genomic clone drrg49-c encompassing one drrg structural gene, the expression of which is correlated with the expression of disease resistance, was sequenced and characterized. the 2.3-kb genomic segment sequenced encompassed 986 bp 5' to the major transcriptional initiation site, a 474-bp open-reading frame interrupted by one 88-bp at-rich intron and an additional ... | 1990 | 2132027 |
| infection with fusarium species in two children with neuroblastoma. | two cases of fusarium infection in children with neuroblastoma are reported. one of the patients had an overwhelming infection and the diagnosis was based on isolation of fusarium moniliforme from blood and skin biopsy, and histological findings. the second patient developed chronic polyarthritis and fusarium solani was cultured from synovial fluid samples taken from two different joints four months apart. no histological documentation of infection was obtained. the response to antifungal therap ... | 1990 | 2148147 |
| mycoflora and nutritional value of shelled melon seeds (citrulus vulgaris schrad.) in nigeria. | thirteen fungi were isolated from mouldy shelled melon seeds. the fungi more frequently isolated included species of mucor (11.25%), rhizopus (13.75%), aspergillus (36.25%), macrophoma (2.50%), penicillium (8.75%), alternaria (5.00%), fusarium (8.75%), botrytis (6.25%), torula (3.75%) and geotrichum (3.75%). healthy, shelled melon seeds inoculated with fusarium solani for 7 or 14 days caused increases in the free fatty acid (f.f.a.) content of the healthy seeds from an initial value of 1.06% to ... | 1990 | 2170965 |
| cutinase is not required for fungal pathogenicity on pea. | cutinase, a fungal extracellular esterase, has been proposed to be crucial in the early events of plant infection by many pathogenic fungi. to test the long-standing hypothesis that cutinase of nectria haematococca (fusarium solani f sp pisi) is essential to pathogenicity, we constructed cutinase-deficient mutants by transformation-mediated gene disruption of the single cutinase gene of a highly virulent n. haematococca strain. four independent mutants were obtained lacking a functional cutinase ... | 1992 | 1392588 |
| isolation and analysis of a novel inducible pectate lyase gene from the phytopathogenic fungus fusarium solani f. sp. pisi (nectria haematococca, mating population vi). | a pectate lyase produced by fusarium solani f. sp. pisi (nectria haematococca, mating population vi) was previously shown to be essential for host infection (m. s. crawford and p. e. kolattukudy, arch. biochem. biophys. 258:196-205, 1987). pectate lyase genes have not been cloned from any phytopathogenic fungi. a gene, designated pela, encoding an inducible pectate lyase was isolated from f. solani f. sp. pisi. a probe was synthesized by polymerase chain reaction with oligonucleotide primers bas ... | 1992 | 1400187 |
| crystallization and preliminary x-ray study of a recombinant cutinase from fusarium solani pisi. | recombinant cutinase from fusarium solani pisi is expressed and excreted with very high yields in escherichia coli cultures. cutinase was crystallized at 20 degrees c using the vapour diffusion technique, with polyethylene glycol 6000 as precipitant. best crystals were obtained at ph 7.0 with polyethylene glycol 6000 as precipitant. best crystals were obtained at ph 7.0 with polyethylene glycol at 15 to 20%. they are monoclinic, with space group p2(1) and cell dimensions a = 35.1 a, b = 67.4 a, ... | 1990 | 2213880 |
| naphthoquinone pigments related to fusarubin from the fungus fusarium solani (mart.) sacc. | a review is presented on the naphthoquinone pigments produced by the filamentous fungus fusarium solani (mart.) sacc., and related species. after description of the naphthoquinone structures and biogenesis, the physiological and genetical controls of pigment production are discussed. the biological properties of the main naphthoquinone pigments so far investigated are described. many problems still remain to be resolved in these fields, where nectria haematococca (berk. and br.) wr., the sexual ... | 1990 | 2233399 |
| evaluation of basidiomycete and deuteromycete (fungi imperfecti) extracts for shared allergenic determinants. | aqueous extracts of select members of the basidiomycetes and deuteromycetes (fungi imperfecti) were evaluated for the presence of shared allergenic determinants using skin prick and radio-allergosorbent test (rast) inhibition. twenty adults with perennial symptoms of rhinitis, with or without asthma, were skin-prick tested with six species of deuteromycetes and seven species of basidomycetes. positive weal-and-flare reactivity to pleurotus ostreatus was associated with alternaria alternata, fusa ... | 1990 | 2253084 |
| disseminated infection caused by fusarium solani in a patient with aplastic anemia. | | 1990 | 2277679 |
| [antagonism in vitro among phytopathogenic and saprobic fungi from horticultural soils]. | two methods were tested in order to determine the existence of in vitro antagonism among saprobic and pathogenic fungi. these microorganisms were the most common isolates from horticultural soils of la plata (buenos aires). trichoderma harzianum; t. koningii and penicillium sp. were antagonistic to all the pathogenic fungi tested, fusarium solani; f. oxysporum; alternaria solani; colletotrichum sp. and sclerotium rolfsii spicaria sp., paecilomyces sp. and chaetomiun sp. were antagonistic only to ... | 1990 | 2287718 |
| acute bovine pulmonary emphysema caused by the fungus fusarium semitectum. | acute bovine pulmonary emphysema (abpe) is known to occur throughout the world. the documented causes include 3-methylindole, 4-ipomeanol and perilla ketone. although 4-ipomeanol is a phytoallexin from fusarium solani, this is the first reported incidence of toxicity involving fusarium semitectum. this report describes the poisoning of cattle consuming fusarium semitectum contaminated pink half-runner bean refuse. | 1988 | 3388752 |
| sti35, a stress-responsive gene in fusarium spp. | a stress-induced mrna was identified in the phytopathogenic fungus fusarium oxysporum f. sp. cucumerinum. treatment of the fungus with ethanol resulted in the induction of a major mrna species encoding a protein of approximate mr 37,000. a full-length cdna clone of the induced message was obtained. rna blot analysis indicated that the mrna was induced by various other stresses, including treatment with copper(ii) chloride and heat (37 degrees c). however, it was not greatly induced by treatment ... | 1990 | 2376567 |
| light-driven diurnal zonation in the filamentous fungus fusarium solani. | zonation in growing mycelia of fusarium solani was induced by diurnal light/dark cycles. only those parts of the hyphae that grew in darkness for less than 20 hours developed a zone of conidia after illumination. in continuous darkness, in continuous illumination, or after a transition from light to darkness, a conidiation zone failed to appear. only light periods exceeding a few seconds but lasting less than 21 hours during a 24 hour light/dark cycle induced zonation. this zonation was not caus ... | 1990 | 2386731 |
| the value of calcofluor white in the diagnosis of mycotic and acanthamoeba infections of the eye and ocular adnexa. | in search of an easy and quick method for the diagnosis of fungal and acanthamoeba infections of the eye, the authors have used calcofluor white (cfw), a fluorescent brightener with marked affinity for chitin and cellulose which are present in the cell walls of fungi and cysts of acanthamoeba. paraffin-embedded tissues containing mycotic organisms were stained with cfw and examined under a fluorescent microscope. several fungi such as candida sp, aspergillus sp, phycomycetes, fusarium solani, hi ... | 1987 | 2436125 |
| fusarium solani: evidence for common antigenic/allergenic determinants with other fungi imperfecti. | aqueous extracts of fusarium solani and other members of the fungi imperfecti were evaluated for the presence of common antigenic/allergenic determinants using skin-prick testing, radio-allergo-sorbent test (rast) inhibition, and immunoelectrophoretic methods. prevalence of skin reactivity in forty-four atopic individuals, tested with commercially available fungal extracts, ranged from 27.3% for alternaria tenuis to 6.8% for penicillium notatum. no specific patterns of reactivity emerged from st ... | 1987 | 2438068 |
| [search for actinomycetes--antagonists of fungi causing sugar beet root rot]. | actinomycetes belonging to streptomyces were isolated from the rhizosphere of sugar beet grown on an infected area after cultivation for many years. 44.1 per cent of them proved to be antagonists of phytopathogenic test fungi. the majority of the antagonists were detected among the cinereus. the lowest number of the antagonists was detected among the azureus, the maximum number of the antagonists was observed in the middle and at the end of the sugar beet vegetation period. during various period ... | 1989 | 2534467 |
| fungemia due to fusarium solani in an immunocompromised child. | | 1986 | 3523454 |
| molecular transformation of fusarium solani with an antibiotic resistance marker having no fungal dna homology. | a vector was constructed for transformation of the plant pathogenic fungus fusarium solani. the promoter 35sp, from cauliflower mosaic virus, was fused to the bacterial gene aph(3')ii, which confers resistance to the aminoglycoside antibiotic g418. two transformation procedures were developed: one using isolated fungal protoplasts, the other using germinated fungal spores. a transformation frequency of 3.3 g418-resistant colonies were obtained per microgram dna. of 14 colonies analyzed, 12 had v ... | 1989 | 2550150 |
| fusariosis in the shrimp penaeus semisulcatus cultured in israel. | the first case of a mycotic infection in shrimp in israel is reported. fusarium solani produced a large melanized lesion in a specimen of penaeus semisulcatus cultured at eilat, on the red sea. fungal hyphae elicited a strong hemocytic response in cuticular and connective tissues. in the underlying muscle, the inflammatory reaction appeared weaker, suggesting a gradual failure by the host to resist mycelial invasion. three cases of human keratomycosis by f. solani have been reported in recent ye ... | 1989 | 2594048 |
| characterization of the termini of linear plasmids from nectria haematococca and their use in construction of an autonomously replicating transformation vector. | the mitochondria of isolate fs37 from nectria haematococca mating population i (fusarium solani f. sp. cucurbitae) contain two linear plasmids, pfsc1 and pfsc2, of 9.2 and 8.3 kbp, respectively. evidence for a protein blocking the 5' termini of these plasmids was obtained from exonuclease digestion experiments. a single protein band with an apparent mr of 80 k was labeled when the dna-protein complex of either plasmid was reacted with [125i] bolton-hunter reagent and then digested with dnase i. ... | 1989 | 2598274 |
| potted plants in hospitals as reservoirs of pathogenic fungi. | the soils of five potted plants cultivated within a hospital were investigated for the presence of fungal opportunistic pathogens of humans. a total of 16 potentially pathogenic species were isolated, including aspergillus fumigatus at up to 53.5 colony-forming units (cfu) per gram dry soil and scedosporium apiospermum (pseudallescheria boydii) at up to 97.0 cfu/g. other common species included phialophora verrucosa and fusarium solani. scedosporium inflatum, a recently described emerging pathog ... | 1989 | 2671744 |
| structure of the cutinase gene and detection of promoter activity in the 5'-flanking region by fungal transformation. | the cutinase gene from fusarium solani f. sp. pisi (nectria hematococa) was cloned and sequenced. sau3a fragments of genomic dna from the fungus were cloned in a lambda charon 35 vector. when restriction fragments generated from the inserts were screened with 5' and 3' probes from cutinase cdna, a 5.5-kilobase ssti fragment hybridized with both probes, suggesting the presence of the entire cutinase gene. a 2,818-base pair segment was sequenced, revealing a 690-nucleotide open reading frame that ... | 1989 | 2703464 |
| fatal fusarium solani infections in baby sharks. | the occurrence of fatal fusariosis in baby bonnethead sharks (sphyrna tiburo) born at the national aquarium, baltimore, maryland, is reported. an atypical strain of fusarium solani was cultured from the tissues of two of the infected sharks following postmortem examination. histopathology revealed an apparent predilection of the fungus for hyaline cartilage. invasion of the cartilage resulted in hyphae with a distorted morphology. in slide culture the fungus displayed the unusual characteristic ... | 1989 | 2746438 |
| the cellulase of penicillium pinophilum. synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases. | two immunologically unrelated cellobiohydrolases (i and ii), isolated from the extracellular cellulase system elaborated by the fungus penicillum pinophilum, acted in synergism to solubilize the microcrystalline cellulose avicel; the ratio of the two enzymes for maximum rate of attack was approx. 1:1. a hypothesis to explain the phenomenon of synergism between two endwise-acting cellobiohydrolases is presented. it is suggested that the cellobiohydrolases may be two stereospecific enzymes concern ... | 1986 | 3707549 |
| antifungal susceptibility of 44 clinical isolates of fusarium species determined by using a broth microdilution method. | the mics and minimum fungicidal concentrations of amphotericin b, natamycin, miconazole, itraconazole, and flucytosine against 17 isolates of fusarium solani, 14 isolates of fusarium moniliforme, 10 isolates of fusarium oxysporum, and 3 isolates of fusarium semitectum were determined by a broth microdilution method. amphotericin b and natamycin were the most active agents tested and failed to show any inoculum size effect. in contrast, miconazole and itraconazole showed poor inhibitory and fungi ... | 1989 | 2817867 |
| fusarium oxysporum keratitis. | we describe herein a patient with a rare occurrence of mycotic keratitis caused by fusarium oxysporum. the fungus destroyed the cornea, and a perforating corneal graft had to be performed. the drug of choice in such diagnosed cases is natamycin. a comparison between fusarium oxysporum and fusarium solani is discussed. | 1989 | 2919034 |
| purification and characterization of cutinase from a fluorescent pseudomonas putida bacterial strain isolated from phyllosphere. | cutinase, an extracellular enzyme, was induced by cutin in a fluorescent pseudomonas putida strain that was found to be cohabiting with an apparently nitrogen-fixing corynebacterium. this enzyme was purified from the culture fluid by acetone precipitation followed by chromatography on deae-cellulose, qae-sephadex, sepharose 6b, and sephadex g-100. the purified enzyme showed a single band when subjected to polyacrylamide electrophoresis and the enzymatic activity coincided with the protein band. ... | 1988 | 3130804 |
| invasive fusarium solani infections in patients with acute leukemia. | two cases of disseminated hyalohyphomycosis due to fusarium solani in patients with acute nonlymphocytic leukemia were studied. the clinical features in both patients included fever, fungemia, severe myalgias, disseminated ecthyma gangrenosum-like skin lesions, ocular symptoms, and a fatal outcome despite systemic administration of amphotericin b in the setting of profound, persistent granulocytopenia. in vitro studies showed the resistance of both f. solani isolates to amphotericin b, 5-fluoroc ... | 1988 | 3164881 |
| invasive fusariosis associated with an injury by a stingray barb. | a previously healthy adult male suffered a wound to the dorsal ulnar aspect of his right hand by a stingray barb while fishing off the east coast of florida. two weeks after the imbedded barb had been surgically removed, an erythematous lesion developed around the wound. histopathologic and microbiological studies revealed infection caused by fusarium solani. the patient was successfully treated with debridement and skin grafting in conjunction with ketoconazole therapy. | 1990 | 2213436 |
| mycoflora of air-conditioners dust from riyadh, saudi arabia. | using the hair baiting technique, 6 genera and 14 species were collected on sabouraud's dextrose agar from 37 dust samples from air-conditioners. the most common fungi were chrysosporium tropicum, c. indicum, c. keratinophilum, aspergillus flavus followed by acremonium strictum and scopulariopsis brevicaulis. using the dilution-plate method, 26 genera and 52 species were collected from 37 dust samples on glucose-(23 genera and 45 species) and cellulose-(18 genera and 34 species) czapek's agar at ... | 1988 | 3236219 |
| disseminated cutaneous fusarium infection with vascular invasion in a leukemic patient. | a 61-year-old male with leukemia manifested multiple cutaneous nodules on his whole body surface, subcutaneous nodules on his arms and a tongue tumor. septate hyphae were observed microscopically in scrapings from the surfaces of the cutaneous lesions. fusarium solani and f. anthophilum were isolated from scrapings from the several skin lesions. histological examination revealed the presence of numerous septate hyphae in the lumina of vessels in the dermis. the fungal elements in the cutaneous t ... | 1987 | 3302198 |
| fusarium solani keratitis treated with natamycin (pimaricin): eighteen consecutive cases. | | 1972 | 4537911 |
| pectate lyase from fusarium solani f. sp. pisi: purification, characterization, in vitro translation of the mrna, and involvement in pathogenicity. | since indirect experimental evidence suggested that penetration of fusarium solani f. sp. pisi into its host (pisum sativum) involved pectin-degrading enzymes (w. köller, c. r. allan, and p. e. kolattukudy (1982) physiol, plant pathol. 20, 47-60), direct tests were made for the production of such degradative enzymes by this pathogen. when the organism was grown on pectin, a pectate lyase (ec 4.2.2.2) was released into the media. this lyase was purified to apparent homogeneity from the culture fi ... | 1987 | 3310898 |