static and dynamic protein impact on electronic properties of light-harvesting complex lh2.a comparative analysis of the temperature dependence of the absorption spectra of the lh2 complexes from different species of photosynthetic bacteria, i.e., rhodobacter sphaeroides, rhodoblastus acidophilus, and phaeospirillum molischianum, was performed in the temperature range from 4 to 300 k. qualitatively, the temperature dependence is similar for all of the species studied. the spectral bandwidths of both b800 and b850 bands increases with temperature while the band positions shift in oppos ...200819367872
[cloning and prokaryotic expression of rhodoblastus acidophilus 5-aminolevlinate synthase gene].5-aminolevulinic acid (ala) is formed by the enzyme ala synthase (alas). however, the fidelity of alas gene among species is low. the alas gene of photosynthetic bacteria rhodoblastus acidophilus was cloned from its genomic dna by conventional pcr and veterette pcr and further sequenced. the identity of alas gene among photosynthetic bacteria species is from 64.0% to 95.1% according to phylogenic analysis. furthermore, the alas gene was subcloned into an expression vector pqe30. for the overprod ...200717944364
rhodoblastus sphagnicola sp. nov., a novel acidophilic purple non-sulfur bacterium from sphagnum peat isolate of purple non-sulfur bacteria was obtained from an acidic sphagnum peat bog and designated strain rs(t). the colour of cell suspensions of this bacterium growing in the light under anaerobic conditions is purplish red. cells of strain rs(t) are rod-shaped, 0.8-1.0 microm wide and 2.0-6.0 microm long, motile by means of polar flagella, reproduce by budding and have a tendency to form rosette-like clusters in older cultures. the cells contain lamellar intracytoplasmic membranes underlyi ...200616738120
ftir-spectral analysis of two photosynthetic hydrogen-producing [corrected] strains and their extracellular polymeric substances.the fourier transform infrared (ftir) spectra of the cells of two photosynthetic h(2)-producing strains, rhodoblastus acidophilus and rhodobacter capsulatus, as well as their extracellular polymeric substances (eps), were evaluated. the ftir spectra of r. capsulatus and its eps during its cultivation were also recorded. the main peaks in the spectra, including 1,080 cm(-1) (carbohydrates), 1,250 cm(-1) (nucleic acids), 2,830-2,930 cm(-1) (lipids), 1,660-1,535 cm(-1) (amide i and ii of proteins), ...200616767463
identification of active methylotroph populations in an acidic forest soil by stable-isotope probing.stable-isotope probing (sip) is a culture-independent technique that enables the isolation of dna from micro-organisms that are actively involved in a specific metabolic process. in this study, sip was used to characterize the active methylotroph populations in forest soil (ph 3.5) microcosms that were exposed to (13)ch(3)oh or (13)ch(4). distinct (13)c-labelled dna ((13)c-dna) fractions were resolved from total community dna by cscl density-gradient centrifugation. analysis of 16s rdna sequence ...200212177327
transfer of rhodopseudomonas acidophila to the new genus rhodoblastus as rhodoblastus acidophilus gen. nov., comb. nov.rhodopseudomonas acidophila has unique properties among the phototrophic alpha-proteobacteria and is quite distinct from the type species of rhodopseudomonas, rhodopseudomonas palustris. therefore, the transfer of rhodopseudomonas acidophila to rhodoblastus acidophilus gen. nov., comb. nov., is proposed. this proposal is in accordance with other taxonomic reclassifications proposed previously and fully reflects the phylogenetic distance from rhodopseudomonas palustris.200111594619
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