| polyamine analysis for chemotaxonomy of thermophilic eubacteria: polyamine distribution profiles within the orders aquificales, thermotogales, thermodesulfobacteriales, thermales, thermoanaerobacteriales, clostridiales and bacillales. | cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by hplc and gc analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. spermidine and a quaternary branched penta-amine, n4-bis(aminopropyl)norspermidine, were the major polyamine in thermocrinis, hydrogenobacter, hydrogenobaculum, aquifex, persephonella, sulfurihydrogenibium, hydrogenothermus, balnearium and thermovibrio, located in the order aquificales. thermo ... | 2004 | 15747232 |
| co(2) uptake and fixation by a thermoacidophilic microbial community attached to precipitated sulfur in a geothermal spring. | carbon fixation at temperatures above 73 degrees c, the upper limit for photosynthesis, is carried out by chemosynthetic thermophiles. yellowstone national park (ynp), wyoming possesses many thermal features that, while too hot for photosynthesis, presumably support chemosynthetic-based carbon fixation. to our knowledge, in situ rates of chemosynthetic reactions at these high temperatures in ynp or other high-temperature terrestrial geothermal springs have not yet been reported. a microbial comm ... | 2009 | 19429558 |
| arsenite-oxidizing hydrogenobaculum strain isolated from an acid-sulfate-chloride geothermal spring in yellowstone national park. | an arsenite-oxidizing hydrogenobaculum strain was isolated from a geothermal spring in yellowstone national park, wyo., that was previously shown to contain microbial populations engaged in arsenite oxidation. the isolate was sensitive to both arsenite and arsenate and behaved as an obligate chemolithoautotroph that used h(2) as its sole energy source and had an optimum temperature of 55 to 60 degrees c and an optimum ph of 3.0. the arsenite oxidation in this organism displayed saturation kineti ... | 2004 | 15006819 |
| new isolates and physiological properties of the aquificales and description of thermocrinis albus sp. nov. | the ecology of the aquificales was studied using a combination of phylogenetic and cultivation approaches. enrichment cultures were prepared from low-salt and marine samples of geothermally and volcanically heated environments of the united states (yellowstone national park), russia (kamchatka), italy, germany, djibouti, iceland, and africa (lake tanganyika). isolation of single cells using the selected cell cultivation technique resulted in 15 different pure cultures. comparisons of their 16s r ... | 2002 | 12215816 |
| hydrogenothermus marinus gen. nov., sp. nov., a novel thermophilic hydrogen-oxidizing bacterium, recognition of calderobacterium hydrogenophilum as a member of the genus hydrogenobacter and proposal of the reclassification of hydrogenobacter acidophilus as hydrogenobaculum acidophilum gen. nov., comb. nov., in the phylum 'hydrogenobacter/aquifex'. | a novel thermophilic, hydrogen-oxidizing bacterium, vm1t, has been isolated from a marine hydrothermal area of vulcano island, italy. cells of the strain were gram-negative rods, 2-4 microm long and 1-1.5 microm wide with four to seven monopolarly inserted flagella. cells grew chemolithoautotrophically under an atmosphere of h2/co2 (80:20) in the presence of low concentrations of o2 (optimum 1-2%). carbohydrates and peptide substrates were not utilized, neither for energy generation nor as a sou ... | 2001 | 11594618 |
| comparative geochemical and microbiological characterization of two thermal pools in the uzon caldera, kamchatka, russia. | arkashin schurf (arkashin) and zavarzin spring (zavarzin), two active thermal pools in the uzon caldera, kamchatka, russia, were studied for geochemical and microbiological characterization. arkashin, the smaller of the two pools, had broader temperature and ph ranges, and the sediments had higher concentrations of total as (4,250 mg/kg) relative to zavarzin (48.9 mg/kg). glycerol dialkyl glycerol tetraether profiles represented distinct archaeal communities in each pool and agreed well with pre ... | 2011 | 22124570 |
| cloning and in situ expression studies of the hydrogenobaculum arsenite oxidase genes. | novel arsenite [as(iii)] oxidase structural genes (aoxab) were cloned from hydrogenobaculum bacteria isolated from an acidic geothermal spring. reverse transcriptase pcr demonstrated expression throughout the outflow channel, and the aoxb cdna clones exhibited distribution patterns relative to the physicochemical gradients in the spring. microelectrode analyses provided evidence of quantitative as(iii) transformation within the microbial mat. | 2009 | 19304831 |
| linking microbial oxidation of arsenic with detection and phylogenetic analysis of arsenite oxidase genes in diverse geothermal environments. | the identification and characterization of genes involved in the microbial oxidation of arsenite will contribute to our understanding of factors controlling as cycling in natural systems. towards this goal, we recently characterized the widespread occurrence of aerobic arsenite oxidase genes (aroa-like) from pure-culture bacterial isolates, soils, sediments and geothermal mats, but were unable to detect these genes in all geothermal systems where we have observed microbial arsenite oxidation. co ... | 2009 | 19196273 |
| complete and draft genome sequences of six members of the aquificales. | the aquificales are widespread in marine and terrestrial hydrothermal environments. here, we report the complete and draft genome sequences of six new members of the aquificales: two marine species, persephonella marina strain ex-h1 and hydrogenivirga strain 128-5-r1 (from the east pacific rise, 9 degrees 50.3'n, 104 degrees 17.5'w, and the eastern lau spreading center, 176 degrees 11.5'w, 20 degrees 45.8's, respectively), and four terrestrial isolates, sulfurihydrogenibium azorense strain az-fu ... | 2009 | 19136599 |
| relative importance of h2 and h2s as energy sources for primary production in geothermal springs. | geothermal waters contain numerous potential electron donors capable of supporting chemolithotrophy-based primary production. thermodynamic predictions of energy yields for specific electron donor and acceptor pairs in such systems are available, although direct assessments of these predictions are rare. this study assessed the relative importance of dissolved h(2) and h(2)s as energy sources for the support of chemolithotrophic metabolism in an acidic geothermal spring in yellowstone national p ... | 2008 | 18641166 |
| molecular characterization of the diversity and distribution of a thermal spring microbial community by using rrna and metabolic genes. | the diversity and distribution of a bacterial community from coffee pots hot spring, a thermal spring in yellowstone national park with a temperature range of 39.3 to 74.1 degrees c and ph range of 5.75 to 6.91, were investigated by sequencing cloned pcr products and quantitative pcr (qpcr) of 16s rrna and metabolic genes. the spring was inhabited by three aquificae genera--thermocrinis, hydrogenobaculum, and sulfurihydrogenibium--and members of the alpha-, beta-, and gammaproteobacteria, firmic ... | 2008 | 18539788 |
| effects of abiotic factors on the phylogenetic diversity of bacterial communities in acidic thermal springs. | acidic thermal springs offer ideal environments for studying processes underlying extremophile microbial diversity. we used a carefully designed comparative analysis of acidic thermal springs in yellowstone national park to determine how abiotic factors (chemistry and temperature) shape acidophile microbial communities. small-subunit rrna gene sequences were pcr amplified, cloned, and sequenced, by using evolutionarily conserved bacterium-specific primers, directly from environmental dna extract ... | 2007 | 17220248 |