| laribacter hongkongensis gen. nov., sp. nov., a novel gram-negative bacterium isolated from a cirrhotic patient with bacteremia and empyema. | a bacterium was isolated from the blood and empyema of a cirrhotic patient. the cells were facultatively anaerobic, nonsporulating, gram-negative, seagull shaped or spiral rods. the bacterium grows on sheep blood agar as nonhemolytic, gray colonies 1 mm in diameter after 24 h of incubation at 37 degrees c in ambient air. growth also occurs on macconkey agar and at 25 and 42 degrees c but not at 4, 44, and 50 degrees c. the bacterium can grow in 1 or 2% but not 3, 4, or 5% nacl. no enhancement of ... | 2001 | 11724825 |
| use of cefoperazone macconkey agar for selective isolation of laribacter hongkongensis. | a new selective medium, cefoperazone macconkey agar (cma), was developed for primary isolation of laribacter hongkongensis from stool. its performance in quantitative recovery and in a clinical evaluation of 4,741 human diarrheal stool specimens was superior to that of charcoal cefoperazone deoxycholate agar. in addition, with cma, arcobacter butzleri was unexpectedly isolated from the stools of six patients. | 2003 | 14532237 |
| laribacter hongkongensis: a potential cause of infectious diarrhea. | in this study, we describe the isolation of laribacter hongkongensis, a recently described genus and species of bacterium, in pure culture on charcoal cefoperazone deoxycholate agar from the stool of six patients with diarrhea. three patients were residents of hong kong, and three of switzerland. in none of the stool samples obtained from these six patients was salmonella, shigella, enterohemorrhagic escherichia coli, vibrio, aeromonas, plesiomonas, or campylobacter recovered. rotavirus antigen ... | 2003 | 14711474 |
| does laribacter hongkongensis cause diarrhoea, or does diarrhoea "cause" l hongkongensis? | | 2004 | 15194250 |
| association of laribacter hongkongensis in community-acquired gastroenteritis with travel and eating fish: a multicentre case-control study. | laribacter hongkongensis has been recovered from several patients with gastroenteritis. however, the causative role of this organism in human gastroenteritis is still unproven, and sources of the bacterium are unknown. we undertook a multicentre case-control study to investigate the association of l hongkongensis with gastroenteritis. | 2004 | 15194253 |
| ecoepidemiology of laribacter hongkongensis, a novel bacterium associated with gastroenteritis. | in a territory-wide surveillance study, laribacter hongkongensis was isolated solely from freshwater fish (60% of grass carps, 53% of bighead carps, and 25% of mud carps). comparing the pulsed-field gel electrophoresis patterns of fish and patient isolates revealed that most patient isolates were clustered together, suggesting that some clones could be more virulent. | 2005 | 15695706 |
| cloning and characterization of a chromosomal class c beta-lactamase and its regulatory gene in laribacter hongkongensis. | laribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most beta-lactams except the carbapenems. we describe the cloning and characterization of a novel chromosomal class c beta-lactamase and its regulatory gene in l. hongkongensis. two genes, ampc and ampr, were cloned by inserting restriction fragments of genomic dna from l. hongkongensis strain hlhk5 into pbk-cmv to give the recombinant plasmid ... | 2005 | 15855519 |
| current status and future directions for laribacter hongkongensis, a novel bacterium associated with gastroenteritis and traveller's diarrhoea. | despite extensive investigations, a microbiological cause cannot be found in about half of the patients with infectious disease. throughout the years, scientists have spent tremendous efforts in looking for microorganisms associated with these "unexplained infectious disease syndromes". recently, a novel bacterium, laribacter hongkongensis, was discovered and shown to be associated with gastroenteritis and traveller's diarrhoea. this review summarizes the current status, and shares with the read ... | 2005 | 16148528 |
| construction of an inducible expression shuttle vector for laribacter hongkongensis, a novel bacterium associated with gastroenteritis. | an escherichia coli-laribacter hongkongensis shuttle vector (ppw380) was constructed by ligating the 4701-bp ecori digested fragment of phlhk8 to ecori digested pbk-cmv. an e. coli-l. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of palc2084 to the 8897-bp fragment of ppw380, deletion of the green fluorescent protein gene, and insertion of a multiple clon ... | 2005 | 16165318 |
| seasonal and tissue distribution of laribacter hongkongensis, a novel bacterium associated with gastroenteritis, in retail freshwater fish in hong kong. | laribacter hongkongensis, a recently discovered bacterium associated with community-acquired gastroenteritis, has been found in the intestines of freshwater fish. to better understand the epidemiology and ecology of the bacterium, we carried out a surveillance study to investigate possible seasonal variation in the recovery of l. hongkongensis and its distribution in various organs in retail freshwater fish in hong kong. forty whole freshwater fish of two species (20 grass carps and 20 bighead c ... | 2007 | 16996630 |
| laribacter hongkongensis isolated from a patient with community-acquired gastroenteritis in hangzhou city. | we describe the isolation of laribacter hongkongensis in hangzhou city, people's republic of china. one strain of bacterium, named lhhz242, had many of the same phenotypic and genotypic characteristics as laribacter hongkongensis described in previous publications. this discovery proves that laribacter hongkongensis is also associated with community-acquired gastroenteritis outside hong kong. | 2007 | 17021061 |
| plasmid profile and construction of a small shuttle vector in laribacter hongkongensis. | among 21 human strains of laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. the smallest, 3264-bp plasmid, phlhk19, has only one orf that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a dnaa box, three 18-bp direct repeats and five pairs of inverted repeats. an escherichia coli-l. hongkongensis shuttle vector was constructed by ligating the hindiii-digested phlhk19, containing the replication ... | 2007 | 17609856 |
| characterization of a novel cryptic plasmid, phlhk26, in laribacter hongkongensis. | we report the complete nucleotide sequence and characterization of a cryptic plasmid, phlhk26, recovered from a strain of laribacter hongkongensis isolated from a patient with community acquired gastroenteritis. phlhk26 consists of 8700 bp, with g + c content 51.3%. the copy number (mean +/- sd) is 0.57 +/- 0.07 and it is stable after four passages (about 240 generations) in the absence of selection. there is a predicted origin of replication that consists of a dnaa box and five 22-bp direct rep ... | 2007 | 17619258 |
| isolation of laribacter hongkongensis, a novel bacterium associated with gastroenteritis, from drinking water reservoirs in hong kong. | freshwater fish has been found to be the reservoir of laribacter hongkongensis, a recently discovered bacterium associated with community-acquired gastroenteritis. however, little is known about the ecology of this bacterium in the aquatic environment. we carried out a surveillance study to investigate the presence of l. hongkongensis in water and freshwater fish from 10 drinking water reservoirs in hong kong. | 2007 | 17714383 |
| advances in defining etiology and new therapeutic approaches in acute diarrhea. | defining etiology of acute diarrhea is critical to disease therapy and prevention. in this review we look at recent developments in etiologic agents of acute diarrhea and advances in therapy and prevention of the illness. newly appreciated agents include enterotoxigenic bacteroides fragilis, klebsiella oxytoca and laribacter hongkongensis. atypical enteropathogenic e. coli (epec) strains lacking the gene for epithelial attachment appear to be more important as causes of diarrhea than traditional ... | 2007 | 17825422 |
| distribution and molecular characterization of tetracycline resistance in laribacter hongkongensis. | laribacter hongkongensis is a newly discovered bacterium associated with gastroenteritis and found in freshwater fish. although isolates resistant to tetracycline have been described, their resistance mechanisms have not been studied. | 2008 | 18227089 |
| then and now: use of 16s rdna gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. | in the last decade, as a result of the widespread use of pcr and dna sequencing, 16s rdna sequencing has played a pivotal role in the accurate identification of bacterial isolates and the discovery of novel bacteria in clinical microbiology laboratories. for bacterial identification, 16s rdna sequencing is particularly important in the case of bacteria with unusual phenotypic profiles, rare bacteria, slow-growing bacteria, uncultivable bacteria and culture-negative infections. not only has it pr ... | 2008 | 18828852 |
| isolation of laribacter hongkongensis, a novel bacterium associated with gastroenteritis, from chinese tiger frog. | laribacter hongkongensis is a recently discovered novel bacterium associated with community-acquired gastroenteritis. although the bacterium has been isolated from freshwater fish and natural freshwater environments, it is not known if other freshwater animals could also be a source of l. hongkongensis. in a surveillance study on freshwater food animals (other than fish) in hong kong, l. hongkongensis was isolated from eight of 10 chinese tiger frogs (hoplobatrachus chinensis), a widespread frog ... | 2009 | 19033083 |
| development of a multi-locus sequence typing scheme for laribacter hongkongensis, a novel bacterium associated with freshwater fish-borne gastroenteritis and traveler's diarrhea. | laribacter hongkongensis is a newly discovered, facultative anaerobic, gram-negative, motile, sea gull-shaped rod associated with freshwater fish borne gastroenteritis and traveler's diarrhea. a highly reproducible and discriminative typing system is essential for better understanding of the epidemiology of l. hongkongensis. in this study, a multilocus sequence typing (mlst) system was developed for l. hongkongensis. the system was used to characterize 146 l. hongkongensis isolates, including 39 ... | 2009 | 19178751 |
| susceptibility patterns of clinical and fish isolates of laribacter hongkongensis: comparison of the etest, disc diffusion and broth microdilution methods. | to determine the antibiotic susceptibility patterns of 60 strains of laribacter hongkongensis isolated from humans and fish to eight antibiotics and compare the results obtained from broth microdilution, etest and disc diffusion susceptibility testing. | 2009 | 19202149 |
| emerging infections of the gastrointestinal tract. | infections account for significant gi morbidity and mortality worldwide. new organisms are being identified, associated with diarrhoeal illness and some with other gastrointestinal illness as well. among gi viruses, sapovirus is now recognised to cause diarrhoea, especially in children. a hypervirulent strain of clostridium difficile has caused epidemics in many countries. newly identified bacterial species that may cause diarrhoea include campylobacter concisus, arcobacteria, edwardsiella tarda ... | 2009 | 19258189 |
| environmental surveillance for laribacter hongkongensis, a diarrhoeal pathogen discovered in hong kong. | 1. laribacter hongkongensis was isolated from the midguts and hindguts of 86 (24%) of 360 freshwater fish from retail markets, including grass carp (60%), bighead carp (53%), mud carp (25%), and large-mouth bass (5%). 2. this study is the first to demonstrate the presence of l hongkongensis in natural water environments, with the bacterium being isolated from the waters of six reservoirs, with higher recovery rates in summer and during days of higher water and ambient temperatures. 3. molecular ... | 2009 | 19258631 |
| the complete genome and proteome of laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats. | laribacter hongkongensis is a newly discovered gram-negative bacillus of the neisseriaceae family associated with freshwater fish-borne gastroenteritis and traveler's diarrhea. the complete genome sequence of l. hongkongensis hlhk9, recovered from an immunocompetent patient with severe gastroenteritis, consists of a 3,169-kb chromosome with g+c content of 62.35%. genome analysis reveals different mechanisms potentially important for its adaptation to diverse habitats of human and freshwater fish ... | 2009 | 19283063 |
| [prevalence and antimicrobial susceptibility of laribacter hongkongensis and enterotoxigenic escherichia coli isolated from patients with diarrhea in guangzhou]. | to survey the prevalence of enterotoxigenic escherichia coli (etec) and laribacter hongkongensis (lh) and their drug resistance in diarrhea patients in guangzhou. | 2010 | 20335108 |
| multi-locus sequence typing scheme for laribacter hongkongensis, a novel bacterium associated with freshwater fish-borne gastroenteritis and traveller's diarrhoea. | | 2010 | 20864740 |
| random exploration of the laribacter hongkongensis genome. | | 2010 | 20864741 |
| identification and characterization of integron-associated antibiotic resistant laribacter hongkongensis isolated from aquatic products in china. | laribacter hongkongensis is a recently discovered bacterium associated with gastroenteritis. in this study, a total of 199 isolates of this species obtained from aquatic products (n=462) in guangzhou city, china, were examined for their susceptibility to 19 antimicrobial agents and the presence of antimicrobial resistance integrons. the genetic relatedness of the isolates with integrons was also evaluated. a pcr-based method was used to screen integrons and found that 13 (6.5%) of the isolates h ... | 2010 | 21075469 |
| isolation of laribacter hongkongensis from little egrets (egretta garzetta) in hangzhou, china. | laribacter hongkongensis is well adapted to diverse freshwater environments and is associated with human community-acquired gastroenteritis and traveller's diarrhoea. the study intended to investigate whether l. hongkongensis could be detected in little egrets (egretta garzetta, a widespread aquatic bird) in hangzhou, china. | 2011 | 21299577 |
| aptamer-mediated inhibition of mycobacterium tuberculosis polyphosphate kinase 2. | inorganic polyphosphate (polyp) plays a number of critical roles in bacterial persistence, stress, and virulence. polyp intracellular metabolism is regulated by the polyphosphate kinase (ppk) protein families, and inhibition of ppk activity is a potential approach to disrupting polyp-dependent processes in pathogenic organisms. here, we biochemically characterized mycobacterium tuberculosis (mtb) ppk2 and developed dna-based aptamers that inhibit the enzyme's catalytic activities. mtb ppk2 catal ... | 2011 | 21381755 |
| bacteremia caused by laribacter hongkongensis misidentified as acinetobacter lwoffii: report of the first case in korea. | laribacter hongkongensis is an emerging pathogen in patients with community-acquired gastroenteritis and traveler's diarrhea. we herein report a case of l. hongkongensis infection in a 24-yr-old male with liver cirrhosis complicated by wilson's disease. he was admitted to a hospital with only abdominal distension. on day 6 following admission, he complained of abdominal pain and his body temperature reached 38.6℃. the results of peritoneal fluid evaluation revealed a leukocyte count of 1,180/µl ... | 2011 | 21532861 |
| environmental adaptability and stress tolerance of laribacter hongkongensis: a genome-wide analysis. | | 2011 | 21711489 |
| virulence determinants, drug resistance and mobile genetic elements of laribacter hongkongensis: a genome-wide analysis. | | 2011 | 21711902 |
| general metabolism of laribacter hongkongensis: a genome-wide analysis. | | 2011 | 21711917 |
| detection of laribacter hongkongensis using species-specific duplex pcr assays targeting the 16s rrna gene and the 16s-23s rrna intergenic spacer region (isr). | aims:ôçé for the rapid detection of laribacter hongkongensis, which is associated with human community-acquired gastroenteritis and traveller's diarrhoea, we developed a duplex species-specific pcr assay. methods and results:ôçé full-length of the 16s-23s rrna intergenic spacer region (isr) sequences of 52 l.-áhongkongensis isolates were obtained by pcr-based sequencing. two species-specific primer pairs targeting 16s rrna gene and isr were designed for duplex pcr detection of l.-áhongkongensis. ... | 2011 | 21689224 |
| Structural and functional insight into the mechanism of an alkaline exonuclease from Laribacter hongkongensis. | Alkaline exonuclease and single-strand DNA (ssDNA) annealing proteins (SSAPs) are key components of DNA recombination and repair systems within many prokaryotes, bacteriophages and virus-like genetic elements. The recently sequenced ß-proteobacterium Laribacter hongkongensis (strain HLHK9) encodes putative homologs of alkaline exonuclease (LHK-Exo) and SSAP (LHK-Bet) proteins on its 3.17 Mb genome. Here, we report the biophysical, biochemical and structural characterization of recombinant LHK-Ex ... | 2011 | 21893587 |
| transport genes and chemotaxis in laribacter hongkongensis: a genome-wide analysis. | abstract: | 2011 | 21849034 |