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psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from spitzbergen.using starch as a carbon source at a cultivation temperature of 4 degrees c, a number of gram-negative, aerobic strains was isolated from sea-ice and sea-water samples collected at spitzbergen in the arctic. analysis of the genetic diversity of the novel isolates by random amplification of polymorphic dna (rapd) and eric fingerprinting revealed a homogenic group of biofilm-forming bacteria that contained small extrachromosomal elements. as a representative of the group, strain pull 5.3t, isolate ...200312710624
marine bacteria associated with the korean brown alga, undaria pinnatifida.several marine bacterial strains were isolated from undaria pinnatifida (miyok in korean). sixty-six strains were isolated on r2a agar media at 10 degrees and identified by a phylogenetic analysis of the 16s rrna gene sequences. they were grouped into 10 different sequence types based on the initial sequence analysis of the 5' domain of the gene (approximately 500 bp). full sequences of 16s rrna gene were obtained from one strain in each sequence type and the species-affiliation was determined u ...200617205052
psychromonas aquatilis sp. nov. isolated from water samples obtained in the chilean antarctica.one slightly beige-white pigmented, gram-staining negative, rod-shaped bacterium, strain m1a1t, was isolated from seawater samples obtained in fildes bay, antarctica (62º12´s, 58º57´w). phylogenetic analysis based on nearly full-length 16s rrna gene sequences showed that the isolate shared 98.4 % 16s rrna gene sequence identity to the type strain of the species psychromonas arctica, but less than 97 % 16s rrna gene sequence similarities to all other psychromonas species. dna-dna hybridisation (d ...201728109208
overexpression of cold shock protein a of psychromonas arctica kopri 22215 confers cold-resistance.a polar bacterium was isolated from arctic sea sediments and identified as psychromonas artica, based on 16s rdna sequence. psychromonas artica kopri 22215 has an optimal growth temperature of 10 degrees c and a maximum growth temperature of 25 degrees c, suggesting this bacterium is a psychrophile. cold shock proteins (csps) are induced upon temperature downshift by more than 10 degrees c. functional studies have researched mostly csps of a mesophilic bacterium escherichia coli, but not on thos ...201020169403
identification and expression of the tig gene coding for trigger factor from psychrophilic bacteria with no information of genome sequence available.trigger factor (tf) plays a key role as a molecular chaperone with a peptidyl-prolyl cis-trans isomerase (ppiase) activity by which cells promote folding of newly synthesized proteins coming out of ribosomes. since psychrophilic bacteria grow at a quite low temperature, between 4 and 15 degrees c, tf from such bacteria was investigated and compared with that of mesophilic bacteria e. coli in order to offer an explanation of cold-adaptation at a molecular level. using a combination of gradient pc ...200919459006
highly phosphorylated core oligosaccharide structures from cold-adapted psychromonas arctica.many cold habitats contain plenty of microorganisms that represent the most abundant cold-adapted life forms on earth. these organisms have developed a wide range of adaptations that involve the cell wall of the microorganism. in particular, bacteria enhance the synthesis of unsaturated fatty acids of membrane lipids to maintain the membrane fluidity, but very little is known about the adaptational changes in the structure of the lipopolysaccharides (lpss), the main constituent of the outer leaf ...200818770509
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