| three groups of babesia canis distinguished and a proposal for nomenclature. | two stocks of large babesia from dogs originating in france, transmitted by dermacentor reticulatus, two from north africa, having rhipicephalus sanguineus as vector, and one from south africa, transmitted by haemaphysalis leachi, were compared in cross-immunity tests in dogs and in the indirect fluorescent antibody test (ifat). the french and north african stocks did not immunise against the south african one, while the north african stocks did not protect against a french one. the south africa ... | 1989 | 2655263 |
| babesia canis canis, babesia canis vogeli, babesia canis rossi: differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal rna genes. | the parasites babesia canis and babesia gibsoni (phylum apicomplexa) are responsible for canine babesiosis throughout the world. babesia canis was previously described as a group of three biologically different subspecies, namely b. canis canis, b. canis vogeli, and b. canis rossi. we report partial sequences of small subunit ribosomal rna gene (ssu-rdna) of each subspecies amplified in vitro with primers derived from a semi-conserved region of the ssu-rdna genes in other babesia species. the po ... | 1999 | 10377990 |
| molecular characterisation of babesia canis canis and babesia canis vogeli from naturally infected european dogs. | the morphologically small babesia species isolated from naturally infected dogs in europe, japan, and us are described as babesia gibsoni despite the fact that molecular techniques show that they should be assigned to two or three separate taxons. the morphologically large babesia isolated from dogs in europe, africa, and us were generally classified as b. canis until it was proposed to distinguish three related, albeit genetically distinct subspecies of this genus, namely b. canis canis, b. can ... | 2002 | 12079734 |
| molecular studies on babesia, theileria and hepatozoon in southern europe. part i. epizootiological aspects. | molecular epizootiology of piroplasmids (babesia spp., theileria spp.) and hepatozoon canis was studied in mammals from southern europe (mainly from spain, but also from portugal and france). partial amplification and sequencing of the 18s rrna gene was used for molecular diagnosis. in some particular cases (b. ovis and b. bovis) the complete 18s rrna gene was sequenced. blood samples were taken from domestic animals showing clinical symptoms: 10 dogs, 10 horses, 10 cows, 9 sheep and 1 goat. in ... | 2003 | 12719133 |
| two species of canine babesia in australia: detection and characterization by pcr. | the haemoprotozoan babesia canis has been recognized in australia for many years, and a second, smaller species has recently been discovered. amplification and sequencing of a partial region of the 18s small subunit ribosomal rna (rrna) gene enabled detection and characterization of the large and small canine babesiae of australia for the first time. isolates from northern australia were genetically characterized to be 99% homologous to babesia canis vogeli, confirming previous speculation about ... | 2003 | 12760669 |
| epidemiological survey of babesia species in japan performed with specimens from ticks collected from dogs and detection of new babesia dna closely related to babesia odocoilei and babesia divergens dna. | detection and analysis of babesia species from ticks recovered from dogs in japan were attempted by pcr and nucleotide sequence analysis based on the 18s rrna gene, respectively. a total of 1136 ticks were examined for babesia dna by 18s rrna-based pcr and nucleotide sequencing. partial sequences of babesia canis vogeli dna were detected from six ticks in aomori, nara, hiroshima, oita, and okinawa prefectures; and babesia gibsoni asia-1 dna was also detected in four ticks in osaka, hiroshima, mi ... | 2003 | 12904344 |
| confirmation of occurrence of babesia canis vogeli in domestic dogs in south africa. | the prevalence of babesia infections in domestic dogs in south africa was studied using reverse line blot hybridization and 18s sequence analysis. babesia canis vogeli was confirmed for the first time in domestic dogs in south africa. out of a total of 297 blood samples collected from domestic dogs in bloemfontein, east london, johannesburg, durban and from the onderstepoort veterinary academic hospital, 31 were positive for babesia canis rossi, whereas b. c. vogeli was detected in 13 dogs. none ... | 2004 | 15177716 |
| canine babesiosis in slovenia: molecular evidence of babesia canis canis and babesia canis vogeli. | canine babesiosis, caused by intraerythrocytic babesia spp., is a tick-borne disease of worldwide importance. no information on canine babesiosis has been documented in slovenia. therefore, 238 dogs admitted to the small animal clinic in ljubljana from the years 2000 to 2002 were tested for the presence of babesial parasites in the blood. based on clinical, microscopic and molecular investigations, 14 dogs (5.9%) were determined as being infected with babesiae. clinical signs relating to acute h ... | 2004 | 15210084 |
| first molecular detection of babesia vogeli in dogs from brazil. | the present work describes the detection and first molecular characterization of babesia vogeli in dogs, naturally infected in brazil and even in south america. microscopic examination of giemsa-stained peripheral blood smears collected from dogs originating from four different locations in brazil revealed the presence of large babesia merozoites and trophozoites (>2.5 microm). dna was extracted from infected blood samples and pcr amplifications of the 18s rdna were carried out. as a reference, ... | 2005 | 15619377 |
| geographic distribution of babesiosis among dogs in the united states and association with dog bites: 150 cases (2000-2003). | to identify the geographic distribution of babesiosis among dogs in the united states and determine, for dogs other than american pit bull terriers (apbts), whether infection was associated with a recent dog bite. | 2005 | 16190594 |
| babesia canis vogeli: a novel pcr for its detection in dogs in australia. | babesia canis vogeli is known to cause disease in dogs in australia, and the rapid detection of various subspecies would enable effective treatment and management. a 21 bp oligonucleotide, "bab-f" was proposed for the production of larger pcr products with high species specificity that would enable effective sequence analyses to yield subspecies identification. the new forward primer when paired with a previously reported "babesia common" reverse primer generated a 394 bp product which was succe ... | 2006 | 16256109 |
| seroepidemiology of canine babesiosis and ehrlichiosis in a hospital population. | canine ehrlichiosis and babesiosis have a worldwide distribution with geographic variation in prevalence and main clinical manifestations. we prospectively determined seroprevalence of canine babesiosis and ehrlichiosis, and risk factors for seropositivity. three hundred and eighty-one dogs were randomly selected to represent the canine population at a veterinary teaching hospital in south brazil (latitude 23 degrees s). dogs were tested with a point-of-care elisa for ehrlichia canis antibodies ... | 2006 | 16647817 |
| detection of anaplasma platys and babesia canis vogeli and their impact on platelet numbers in free-roaming dogs associated with remote aboriginal communities in australia. | to detect anaplasma platys and babesia canis vogeli infection, using polymerase chain reaction (pcr)-based assays, in free-roaming dogs associated with eight aboriginal communities in remote areas of australia and to determine the impact of infection through the assessment of platelet numbers. | 2006 | 16958629 |
| new advances in molecular epizootiology of canine hematic protozoa from venezuela, thailand and spain. | the prevalence of hematozoan infections (hepatozoon canis and babesia sp., particularly babesia canis vogeli) in canids from venezuela, thailand and spain was studied by amplification and sequencing of the 18s rrna gene. h. canis infections caused simultaneously by two different isolates were confirmed by rflp analysis in samples from all the geographic regions studied. in venezuela, blood samples from 134 dogs were surveyed. babesia infections were found in 2.24% of the dogs. comparison of sequ ... | 2007 | 17088022 |
| epidemiological aspects of canine babesiosis in the semiarid area of the state of minas gerais, brazil. | epidemiological aspects of babesia vogeli infection were studied in the canine population of a rural town located in the brazilian "drought polygon" of the state of minas gerais, brazil. the survey was carried out in march 2003, when 505 dogs were identified and their characteristics registered on appropriate forms. blood samples were collected at this time and again in june, september and december 2003. serum samples were tested by the indirect fluorescent antibody test (ifat) to detect antibod ... | 2007 | 17210194 |
| endocrine predictors of mortality in canine babesiosis caused by babesia canis rossi. | this prospective, cross-sectional, observational study was designed to determine the association between the hormones of the pituitary-adrenal and pituitary-thyroid axes and outcome in dogs with naturally occurring babesia canis rossi babesiosis. ninety-five dogs with canine babesiosis were studied and blood samples were obtained from the jugular vein in each dog prior to treatment at admission to hospital. serum cortisol, adrenocorticotrophic hormone (acth), thyroxine, free thyroxine and thyrot ... | 2007 | 17614201 |
| prevalence of ehrlichia canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, bartonella vinsonii berkhoffii, and rickettsia spp. in dogs from grenada. | to identify the tick-borne pathogens in dogs from grenada, we conducted a serologic survey for ehrlichia canis in 2004 (104 dogs) and a comprehensive serologic and molecular survey for a variety of tick-borne pathogens in 2006 (73 dogs). in 2004 and 2006, 44 and 32 dogs (42.3% and 43.8%) were seropositive for e. canis, respectively. in 2006, several tick-borne pathogens were identified by serology and pcr. dna of e. canis, anaplasma platys, babesia canis vogeli, hepatozoon canis, and bartonella ... | 2008 | 18160223 |
| assessment of primers designed for the subspecies-specific discrimination among babesia canis canis, babesia canis vogeli and babesia canis rossi by pcr assay. | canine babesiosis is an infectious disease caused by either babesia gibsoni or babesia canis protozoans. the latter is also classified under three different phylogenetic groups, referred to as subspecies b. canis canis, b. canis vogeli and b. canis rossi. the objective of the present study was to validate and standardize a pcr assay to discriminate the organisms at the subspecies level. first, the reference sequences of the 18s rrna, 5.8s rrna and 28s rrna genes, including the internal transcrib ... | 2008 | 18242863 |
| detection and molecular characterization of babesia canis vogeli from naturally infected dogs and rhipicephalus sanguineus ticks in tunisia. | canine babesiosis, caused by intra-erythrocytic babesia, is a tick-borne disease of worldwide importance. no information on canine babesiosis has been documented in tunisia. detection and analysis of babesia species from naturally infected dogs and ticks recovered from dogs were attempted by reverse line blot hybridization and nucleotide sequence analysis based on 18s rrna gene. out of 180 blood samples collected from domestic dogs in 4 villages situated in different bioclimatic zones, 12 were p ... | 2008 | 18242865 |
| molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in south africa. | a total of 1,138 blood specimens were collected over a 6-year period (2000-2006) from domestic dogs in south africa. specimens from domestic dogs were obtained from the onderstepoort veterinary academic hospital (ovah) in pretoria, the society for the prevention of cruelty to animals (johannesburg, durban, east london and bloemfontein) and private practices from four provinces (gauteng, mpumalanga, kwazulu-natal and western cape). all specimens were screened for babesia, theileria, hepatozoon an ... | 2008 | 18502588 |
| babesia canis canis and babesia canis vogeli infections in dogs from northern portugal. | canine babesiosis represents an important veterinary medical problem. this study describes the molecular characterization of babesial parasites detected in eight clinically suspected dogs from northern portugal, affected by lethargy, muscle tremors, weight loss, pale mucous membranes, hyperthermia or red-coloured urine. microscopic examination of peripheral blood smears showed large intraerythrocytic piroplasms morphologically compatible with babesia canis in all eight animals. dna was extracted ... | 2008 | 18602757 |
| serum cardiac troponin i concentration in dogs with ehrlichiosis. | ehrlichiosis is a multisystemic disease with the potential to cause cardiomyocyte injury in naturally infected dogs. | 2008 | 18638021 |
| canine vector-borne diseases in brazil. | abstract: canine vector-borne diseases (cvbds) are highly prevalent in brazil and represent a challenge to veterinarians and public health workers, since some diseases are of great zoonotic potential. dogs are affected by many protozoa (e.g., babesia vogeli, leishmania infantum, and trypanosoma cruzi), bacteria (e.g., anaplasma platys and ehrlichia canis), and helminths (e.g., dirofilaria immitis and dipylidium caninum) that are transmitted by a diverse range of arthropod vectors, including tick ... | 2008 | 18691408 |
| canine babesiosis caused by babesia canis vogeli in rural areas of the state of minas gerais, brazil and factors associated with its seroprevalence. | this epidemiological survey on canine babesiosis was carried out in three distinct rural regions (lavras, belo horizonte and nanuque) of the state of minas gerais, brazil. ticks and blood samples were collected during a dry season (lavras, n=92; belo horizonte, n=50; nanuque, n=102) and the subsequent rainy season (lavras, n=71; belo horizonte, n=28; nanuque, n=66) from dogs living on farms. plasma samples were analyzed by the indirect fluorescent antibody test for detection of anti-babesia cani ... | 2009 | 18723199 |
| first molecular characterization of babesia vogeli in two naturally infected dogs of buenos aires, argentina. | large piroplasms (>2.5microm) were detected by direct microscopical investigation in 34 out of 16,767 (0.20%) canine blood smears in the southern region of greater buenos aires. genomic dna was extracted from two parasitemic dogs and the hypervariable 18s rna gene region of the pathogen was specifically amplified, sequenced, and aligned with corresponding gene sequences available in the genbank. phylogenetic trees were constructed and compared. 18s rna gene sequences reliably segregated in three ... | 2008 | 18786765 |
| babesia canis canis and babesia canis vogeli clinicopathological findings and dna detection by means of pcr-rflp in blood from italian dogs suspected of tick-borne disease. | the aims of this study were to determine the presence of babesia spp. in blood samples from italian dogs with clinical signs compatible with tick-borne diseases by means of pcr-restriction fragment length polymorphism (rflp) and describe the clinicopathological findings of dogs with babesia infection. we evaluated the majority of canine babesiosis cases by means of clinical history, physical examination, hematological, biochemical, serum electrophoresis, urinalysis and hemostatic tests. forty-fi ... | 2008 | 18789581 |
| hemoprotozoa of domestic animals in france: prevalence and molecular characterization. | very limited information is available on epizootiology of haematozoan infections in french domestic animals. in an attempt to address this issue, prevalence of piroplasmida was studied in carnivores and ruminants, whereas prevalence of hepatozoon spp. was only investigated in carnivores. in total, 383 animals were included in the survey (namely 116 cats, 108 dogs, 91 sheep and 68 cows). parasite diagnosis was carried out using molecular methods such as pcr and sequencing of the 18s rrna gene. in ... | 2009 | 19013719 |
| the application of pcr and reverse line blot hybridization to detect arthropod-borne hemopathogens of dogs and cats in trinidad. | arthropod-borne diseases are important causes of morbidity and mortality of companion animals in trinidad. as clinical signs are vague, more sensitive methods to diagnose these diseases based on the polymerase chain reaction (pcr) followed by reverse line blot hybridization (rlb) of amplified products are being developed. an rlb of 14 oligonucleotide probes coupled with polymerase chain-amplified regions of 16s rrna or 18s rrna genes of hemoparasites from cats and dogs detected ehrlichia canis, ... | 2008 | 19120209 |
| pcr survey of vectorborne pathogens in dogs living in and around barcelona, an area endemic for leishmaniasis. | blood samples from 153 dogs living in and around barcelona were assayed for leishmania infantum and ehrlichia, anaplasma, rickettsia, bartonella, hepatozoon, babesia and theileria species by pcr amplification of dna, and the amplicons obtained were sequenced. the prevalence of the infectious agents was l infantum (29.4 per cent), ehrlichia and anaplasma species (4.0 per cent), hepatozoon canis (3.3 per cent), babesia canis vogeli (2.0 per cent), babesia gibsoni (2.0 per cent), babesia canis cani ... | 2009 | 19168881 |
| detection of erythrocyte binding igm and igg by flow cytometry in sick dogs with babesia canis canis or babesia canis vogeli infection. | the aim of this study was to examine by means of flow cytometry immunophenotyping (fci) if sick dogs infected with babesia canis canis (b. c. canis) or babesia canis vogeli (b. c. vogeli) had anti-erythrocyte membrane binding igg and/or igm at the time of diagnosis. diagnosis of babesia infection was assessed by blood smear and by pcr-restriction fragment length polymorphism analysis in 30 sick dogs. signalment, clinical history, physical examination and laboratory tests of b. c. canis (n=24) an ... | 2009 | 19269745 |
| diversity of babesia and theileria species in symptomatic and asymptomatic dogs in croatia. | babesiosis, the disease caused by tick-borne hematozoan parasites of the genus babesia, is particularly common in dogs, and is caused by several "large" species of babesia, as well as by an increasing number of "small" species of babesia, some of which appear to be more closely related to members of the genus theileria. in this work, blood samples were collected from 848 randomly selected, asymptomatic dogs and from 81 symptomatic dogs, microscopically positive for babesia, and characterised by ... | 2009 | 19367832 |
| diversity ofbabesia and theileria species in symptomatic and asymptomatic dogs in croatia. | babesiosis, the disease caused by tick-borne hematozoan parasites of the genus babesia, is particularly common in dogs, and is caused by several "large" species ofbabesia, as well as by an increasing number of "small" species of babesia, some of which appear to be more closely related to members of the genus theileria. in this work, blood samples were collected from 848 randomly selected, asymptomatic dogs and from 81 symptomatic dogs, microscopically positive for babesia, and characterized by p ... | 2009 | 19640468 |
| ticks and haemoparasites of dogs from praia, cape verde. | in february 2008 an epidemiological field study on arthropod-borne infections in dogs was carried out in praia, the capital city of cape verde. for this purpose 130 dogs were included in the study. of these, 94.6% were infested with ticks. altogether, 1293 ticks of the genus rhipicephalus (in all evaluated cases r. sanguineus) were collected. examination for haemotropic parasites was performed via polymerase chain reaction (pcr). lymph node fine-needle aspirates were screened by pcr for leishman ... | 2009 | 19729247 |
| a pcr study of piroplasms in 166 dogs and 111 horses in france (march 2006 to march 2008). | positive pcr results for piroplasms were obtained on edta blood samples from 166 dogs and 111 horses between march 2006 and march 2008. the organisms were initially identified using common primers, followed by restriction enzyme profiles to determine the species and types of babesia/theileria. in 27 dogs and eight horses with positive results, the size of the specimen was insufficient to separate species/types. we identified babesia canis canis in 105 of the 166 dogs (63%) and theileria equi in ... | 2010 | 20221639 |
| canine babesiosis in northern portugal and molecular characterization of vector-borne co-infections. | abstract: | 2010 | 20377861 |
| survey for tick-borne zoonoses in the state of espirito santo, southeastern brazil. | blood samples collected from 201 humans, 92 dogs, and 27 horses in the state of espirito santo, brazil, were tested by polymerase chain reaction, indirect immunofluorescence assays, and indirect enzyme-linked immunosorbent assay for tick-borne diseases (rickettsiosis, ehrlichiosis, anaplasmosis, borreliosis, babesiosis). our results indicated that the surveyed counties are endemic for spotted fever group rickettsiosis because sera from 70 (34.8%) humans, 7 (7.6%) dogs, and 7 (25.9%) horses were ... | 2010 | 20595502 |
| molecular detection of ehrlichia canis, anaplasma bovis, anaplasma platys, candidatus midichloria mitochondrii and babesia canis vogeli in ticks from israel. | : ticks are vectors of important pathogens of human and animals. therefore, their microbial carriage capacity is constantly being investigated. the aim of this study was to characterize the diversity of domestic animal pathogens in ticks collected from vegetation and the ground, from different parts of israel. non-engorged questing adult ticks were collected from 13 localities. a total of 1196 ticks in 131 pools-83 pools of rhipicephalus turanicus and 48 of rhipicephalus sanguineus (with two to ... | 2011 | 20636417 |
| detection of babesia vogeli in stray cats of metropolitan bangkok, thailand. | the combination of a rapidly growing stray animal population and the lack of animal control in bangkok has resulted in a unique opportunity to evaluate the potential role of companion animals as sentinels and reservoirs of infectious diseases, including several of those caused by vector-borne parasites. the purpose of this study was to determine the prevalence and factors associated with the distribution of babesia species infections among stray cats in bangkok. blood samples were collected from ... | 2010 | 20638794 |
| diagnosis of canine vector-borne diseases in young dogs: a longitudinal study. | canine vector-borne diseases (cvbds) pose a diagnostic challenge, particularly when a dog is coinfected with more than one pathogen. the purpose of this study was to generate information about the diagnosis of cvbds in young dogs following their first exposure to flea, tick, sand fly, louse, and mosquito vectors. from march 2008 to may 2009, 10 purpose-bred young naive beagle dogs and a cohort of 48 mixed-breed dogs living in an area to which cvbd is endemic in southern italy were monitored usin ... | 2010 | 20660218 |
| multiplex real-time qpcr for the detection of ehrlichia canis and babesia canis vogeli. | ehrlichia canis and babesia canis vogeli are two tick-borne canine pathogens with a worldwide importance. both pathogens are transmitted by rhipicephalus sanguineus, the brown dog tick, which has an increasing global distribution. a multiplex quantitative real-time pcr (qpcr) assay for the simultaneous detection of the tick-borne pathogens e. canis and b. canis vogeli was developed using dual-labeled probes. the target genes were the 16s rrna of e. canis and the heat shock protein 70 (hsp70) of ... | 2010 | 20674177 |
| molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan recife (north-eastern brazil). | to identify dna of the main tick-borne pathogens in dogs from recife (brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the veterinary hospital of the universidade federal rural de pernambuco from march 2007 to june 2008. the detection of dna was performed using specific primers. amplicons were analyzed through electrophoresis and sequencing. a phylogenetic tree was constructed using the upgma method, revealing that the sequences were closely related to tho ... | 2010 | 20680344 |
| evolution of clinical, haematological and biochemical findings in young dogs naturally infected by vector-borne pathogens. | longitudinal studies evaluating the evolution of clinical, haematological, biochemical findings in young dogs exposed for the first time to multiple vector-borne pathogens have not been reported. with the objective of assessing the evolution of clinical, haematological and biochemical findings, these parameters were serially monitored in naturally infected dogs throughout a 1-year follow-up period. young dogs, infected by vector-borne pathogens based on cytology or polymerase chain reaction, wer ... | 2010 | 21106311 |
| why is southern african canine babesiosis so virulent? an evolutionary perspective. | abstract: canine babesiosis is a common, highly virulent disease in southern africa with even pups and juveniles being severely affected. this contrasts with bovine babesiosis, for example, where host, parasite and vector co-evolved and young animals develop immunity after infection without showing clinical signs. babesia rossi, the main causative organism of canine babesiosis in sub-saharan africa, was first described from a side-striped jackal (canis adustus) in kenya. although data are meagre ... | 2011 | 21489239 |
| babesiosis in dogs and cats-expanding parasitological and clinical spectra. | canine babesiosis caused by different babesia species is a protozoal tick-borne disease with worldwide distribution and global significance. historically, babesia infection in dogs was identified based on the morphologic appearance of the parasite in the erythrocyte. all large forms of babesia were designated babesia canis, whereas all small forms of babesia were considered to be babesia gibsoni. however, the development of molecular methods has demonstrated that other babesia species such as ba ... | 2011 | 21571435 |
| babesia canis rossi infection in a texas dog. | a 5-month-old intact male boerboel dog, imported from south africa 1 week previously, was presented to a texas veterinarian for lethargy, anorexia, and labored breathing. the dog was febrile, anemic, leukopenic, thrombocytopenic, and slightly azotemic. results of the idexx snap-4dx enzyme immunoassay were negative for dirofilaria immitis antigen and antibodies against ehrlichia canis, borrelia burgdorferi, and anaplasma phagocytophilum. an edta blood sample analyzed at oklahoma state university ... | 2011 | 21790699 |
| a survey of canine tick-borne diseases in india. | abstract: background: there are few published reports on canine babesia, ehrlichia, anaplasma, hepatozoon and haemotropic mycoplasma infections in india and most describe clinical disease in individual dogs, diagnosed by morphological observation of the microorganisms in stained blood smears. this study investigated the occurrence and distribution of canine tick-borne disease (tbd) pathogens using a combination of conventional and molecular diagnostic techniques in four cities in india. results: ... | 2011 | 21771313 |
| molecular detection of hepatozoon canis and babesia canis vogeli in domestic dogs from cuiabá, brazil. | the objective of this study was to report for the first time infection by hepatozoon spp. and babesia spp. in 10 dogs from the city of cuiabá, state of mato grosso, central-western brazil. a pair of primers that amplifies a 574 bp fragment of the 18s rrna of hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18s rrna for babesia spp. were used. six dogs were positive for babesia spp., and 9 were positive for hepatozoon spp. co‑infection of babesia spp. and hepato ... | 2011 | 21961759 |
| epidemiological aspects on vector-borne infections in stray and pet dogs from romania and hungary with focus on babesia spp. | canine arthropod-borne infections are of major interest in small animal practice and have been widely investigated in central and western europe. however, only limited epidemiological data are available from south-eastern european countries, although diseases including babesiosis or dirofilariosis are widely recognised as important canine infections in these countries. a steadily increasing number of dogs imported from south-eastern europe into germany require particular attention by small anima ... | 2011 | 21947342 |
| molecular and serological detection of ehrlichia canis and babesia vogeli in dogs in colombia. | ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by ehrlichia canis and babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, rhipicephalus sanguineus. the present work aimed to detect the presence of e. canis and babesia sp. in 91 dog blood samples in colombia, by molecular and serological techniques. we also performed sequence alignment to indicate the identity of the parasite species infecting these animals. the present wo ... | 2011 | 22130333 |
| Canine babesiosis in Romania due to Babesia canis and Babesia vogeli: a molecular approach. | Canine babesiosis is a tick-borne disease caused by the protozoa Babesia spp. that affects dogs worldwide. In Romania, canine babesiosis has become quite frequent in the last few years, with a wide variety of clinical signs, ranging from mild, nonspecific illness to peracute collapse, and even death. Traditionally, a Babesia infection in dogs is diagnosed based on the morphologic appearance of the intraerythrocytic piroplasms observed in peripheral blood smears. To date, no data on genetic chara ... | 2011 | 22006189 |
| phylogenetic characterization of babesia canis vogeli in dogs in the state of goiás, brazil. | the genus babesia comprises protozoa that cause diseases known as babesiosis. dogs are commonly affected by babesia canis or babesia gibsoni. babesia canis is divided into the subspecies babesia canis canis, babesia canis vogeli and babesia canis rossi. among these, babesia canis vogeli predominates in brazil. the objective of this study was to conduct a phylogenetic analysis on babesia isolates from dogs in goiânia, goiás. blood samples were obtained from 890 dogs presenting clinical signs sugg ... | 2011 | 22166380 |
| first description of natural ehrlichia canis and anaplasma platys infections in dogs from argentina. | bacteria belonging to the anaplasmataceae family are vector transmitted agents that affect a variety of vertebrate hosts including the tick-borne pathogens ehrlichia canis and anaplasma platys, which cause canine monocytic ehrlichiosis and cyclic thrombocytopenia, respectively. these two infections, typically reported from tropical and sub-tropical regions, have not been previously reported in dogs from argentina. a total of 86 blood samples from dogs with suspected rickettsial disease and 28 no ... | 2012 | 23273677 |
| [investigation on babesia in ticks infested on police dogs in selected areas of china]. | 399 tick specimens were collected from the body surface of police dogs in chongqing municipality, provinces of fujian, guangdong, hainan, guangxi, hebei, henan, shanxi, jiangsu and zhejiang. nested-pcr and sequence testing were taken to investigate the prevalence of babesia sp. in ticks. the results showed that babesia vogeli was found in ticks infested on the body surface of police dogs, with a positive rate of 5.3%. the prevalence in chongqing, guangdong, guangxi, hainan and zhejiang was 4/16, ... | 2012 | 23484282 |
| haemoparasites of free-roaming dogs associated with several remote aboriginal communities in australia. | tick-borne haemoparasites babesia vogeli and anaplasma platys are common among the free-roaming canine populations associated with aboriginal communities in australia, whilst the prevalence of haemoplasmas, which are also suspected to be tick-borne, remained unexplored. the aim of this study was to determine the prevalence of haemoplasma infection in these populations, and to identify any correlation with other haemoparasites. blood was collected from 39 dogs associated with four aboriginal comm ... | 2012 | 22584120 |
| feline vector-borne pathogens in the north and centre of portugal. | in recent years, several clinical cases and epidemiological studies of feline vector-borne diseases (fvbd) have been reported worldwide. nonetheless, information on fvbd agents and their prevalence in portugal is scarce. | 2013 | 23587366 |
| bacterial and protozoal agents of feline vector-borne diseases in domestic and stray cats from southern portugal. | feline vector-borne diseases (fvbd) have emerged in recent years, showing a wider geographic distribution and increased global prevalence. in addition to their veterinary importance, domestic cats play a central role in the transmission cycles of some fvbd agents by acting as reservoirs and sentinels, a circumstance that requires a one health approach. the aim of the present work was to molecularly detect feline vector-borne bacteria and protozoa with veterinary and zoonotic importance, and to a ... | 2014 | 24655431 |
| detection of vector-borne pathogens in cats and their ectoparasites in southern italy. | vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy. | 2016 | 27160725 |
| ticks and associated pathogens collected from cats in sicily and calabria (italy). | limited information is available about the species of ticks infesting the cat and the pathogens that they harbor. the aims of the present study were to identify the species of ticks removed from cats living in sicily and calabria (italy) and to detect dna of vector-borne pathogens in the same ticks. | 2015 | 26445916 |
| molecular detection of vector-borne agents in dogs from ten provinces of china. | although many vector-borne agents are potential zoonoses and cause substantial morbidity and mortality in dogs worldwide, there are limited data on these organisms in dogs of china. | 2015 | 26428085 |
| efficacy of an imidacloprid/flumethrin collar against fleas, ticks and tick-borne pathogens in dogs. | tick-borne diseases comprise a group of maladies that are of substantial medical and veterinary significance. a range of tick-borne pathogens, including diverse species of bacteria and protozoa, can infect both dogs and humans. hence, the control of tick infestations is pivotal to decrease or prevent tick-borne pathogen transmission. therefore, different commercial products with insecticidal, repellent or both properties have been developed for use on dogs. recently, a collar containing a combin ... | 2013 | 23972013 |
| molecular detection and characterization of tick-borne pathogens in dogs and ticks from nigeria. | only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in nigeria. the aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from nigeria. | 2013 | 23505591 |
| molecular investigation of tick-borne pathogens in dogs from luanda, angola. | no molecular data have been available on tick-borne pathogens that infect dogs from angola. the occurrence of agents from the genera anaplasma, babesia, ehrlichia and hepatozoon was assessed in 103 domestic dogs from luanda, by means of the polymerase chain reaction (pcr) and dna sequence analysis. | 2016 | 27160839 |
| canine vector-borne pathogens in semi-domesticated dogs residing in northern cambodia. | in southeast asia, the canine vector-borne pathogens babesia spp., ehrlichia canis, anaplasma platys, hepatozoon canis, haemotropic mycoplasmas and dirofilaria immitis cause significant morbidity and mortality in dogs. moreover, dogs have also been implicated as natural reservoirs for rickettsia felis, the agent of flea-borne spotted fever, increasingly implicated as a cause of undifferentiated fever in humans in southeast asia. the objective of this study was to determine the prevalence and div ... | 2016 | 27161452 |
| comparative speed of kill of sarolaner (simparica) and afoxolaner (nexgard) against induced infestations of rhipicephalus sanguineus s.l. on dogs. | the brown dog tick, rhipicephalus sanguineus sensu lato, commonly infests dogs globally, is the major vector of the pathogen that causes canine monocytic ehrlichiosis and also transmits babesia vogeli. a rapid speed of kill of a parasiticide is essential to reduce the direct deleterious effects of tick infestation and the risk of tick-borne pathogen transmission. the speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (simparica), against r. sanguineus sensu lato on ... | 2016 | 26896456 |
| acute febrile illness is associated with rickettsia spp infection in dogs. | rickettsia conorii is transmitted by rhipicephalus sanguineus ticks and causes mediterranean spotted fever (msf) in humans. although dogs are considered the natural host of the vector, the clinical and epidemiological significance of r. conorii infection in dogs remains unclear. the aim of this prospective study was to investigate whether rickettsia infection causes febrile illness in dogs living in areas endemic for human msf. | 2015 | 25886403 |
| detection of dirofilaria immitis and other arthropod-borne filarioids by an hrm real-time qpcr, blood-concentrating techniques and a serological assay in dogs from costa rica. | canine filarioids are important nematodes transmitted to dogs by arthropods. diagnosis of canine filariosis is accomplished by the microscopic identification of microfilariae, serology or pcr for filarial-dna. the aim of this study was to evaluate a molecular assay for the detection of canine filariae in dog blood, to compare its performance to other diagnostic techniques, and to determine the relationship between microfilarial concentration and infection with other vector-borne pathogens. | 2015 | 25851920 |
| molecular analysis of the rrna genes of babesia spp and ehrlichia canis detected in dogs from ribeirão preto, brazil. | the partial dna sequences of the 18s rrna gene of babesia canis and the 16s rrna gene of ehrlichia canis detected in dogs from ribeirão preto, brazil, were compared to sequences from other strains deposited in genbank. the e. canis strain circulating in ribeirão preto is identical to other strains previously detected in the region, whereas the subspecies babesia canis vogeli is the main babesia strain circulating in dogs from ribeirão preto. | 2009 | 24031351 |
| clinical, hematologic, and molecular findings in naturally occurring babesia canis vogeli in egyptian dogs. | background. canine babesiosis is a clinically important hemoprotozoan parasite affecting dogs. the goal of this present study was to determine the clinical symptoms and to establish its hematological and microscopic detection and compare it with the pcr findings attained from dogs infected with babesia canis vogeli. methodology/principal findings. 13-pcr confirmed babesia-infected dogs were examined; seminested pcr was used to discover the precise type of babesia and babesia canis vogeli was the ... | 2014 | 24693460 |
| incidence of cercopithifilaria bainae in dogs and probability of co-infection with other tick-borne pathogens. | cercopithifilaria bainae is a filarioid parasite that infects dogs, being transmitted by rhipicephalus sanguineus group ticks in many countries of the mediterranean basin. this study assessed the incidence density rate (idr) of infection by c. bainae in dogs and the probability of co-infection with other tick-borne pathogens (i.e., anaplasma platys, babesia vogeli and hepatozoon canis), in an area of high endemicity in southern italy. | 2014 | 24498437 |
| detection of mycoplasma haemocanis, mycoplasma haematoparvum, mycoplasma suis and other vector-borne pathogens in dogs from córdoba and santa fé, argentina. | in argentina, only very few reports are available for canine tick-borne diseases where most are related to parasitic diseases. the objective of this survey was to investigate the prevalence of tick-borne pathogens in 70 dogs from santa fé and córdoba, argentina. | 2016 | 27978844 |
| prevalence of vector-borne pathogens in dogs from haiti. | canine vector-borne pathogens are common on some caribbean islands, but survey data in haiti are lacking. to determine the prevalence of selected vector-borne pathogens in dogs from haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with rhipicephalus sanguineus ticks at the time of blood collection. no other tick species were identified on these dogs. a commercially available elisa identified antibodies to ehrlichia spp. in 69 (32.9%), antibodies to ... | 2016 | 27270383 |
| parasites and vector-borne pathogens in client-owned dogs in albania. blood pathogens and seroprevalences of parasitic and other infectious agents. | knowledge on the epidemiology of parasitic and vector-borne infections is still very limited for albania, a country located in the balkan peninsula in southeast europe. recent publications indicated prevalence rates of up to 52% for vector-borne infections in less-cared dogs in albania. to provide data on the epidemiological situation in dogs under veterinary care, a total of 602 client-owned dogs presented to four small animal clinics between march 2010 and april 2011 in tirana, albania, were s ... | 2016 | 26453093 |
| tick-borne diseases (borreliosis, anaplasmosis, babesiosis) in german and austrian dogs: status quo and review of distribution, transmission, clinical findings, diagnostics and prophylaxis. | tick-borne diseases (tbd) in dogs have gained in significance in german and austrian veterinary practices. the widespread european tick species ixodes ricinus represents an important vector for spirochaetes of the borrelia burgdorferi sensu lato group and rickettsiales such as anaplasma phagocytophilum. the meadow or ornate dog tick (dermacentor reticulatus) is an important vector for babesia canis, as is the brown dog tick (rhipicephalus sanguineus) for babesia vogeli in the mediterranean regio ... | 2015 | 26152408 |
| spatial distribution of anaplasma phagocytophilum and hepatozoon canis in red foxes (vulpes vulpes) in hungary. | in recent years, ehrlichia canis and hepatozoon canis transmitted by rhipicephalus sanguineus were reported from hungary. the aim of the present study was to reveal the spatial distribution pattern of pathogens transmitted by r. sanguineus in a sentinel species, red fox (vulpes vulpes) in hungary and to analyse the relationship of these patterns with landscape and climate by geographical information systems. fox carcasses, representing 0.5% of the total fox population were randomly selected out ... | 2015 | 26065623 |
| arthropod-borne pathogens circulating in free-roaming domestic cats in a zoo environment in brazil. | recently, tick and flea-borne pathogens have been detected in wild carnivores maintained in captivity in brazilian zoos. since free-roaming cats are frequently found in brazilian zoos, they could act as reservoirs for arthropod-borne pathogens, which could be transmitted to endangered wild carnivores maintained in captivity in these institutions. on the other hand, stray cats in zoos may play a role as sentinels to pathogens that circulate among wild animals in captivity. the present work aimed ... | 2014 | 24889035 |
| molecular detection of bacterial and parasitic pathogens in hard ticks from portugal. | ticks are important vector arthropods of human and animal pathogens. as information about agents of disease circulating in vectors in portugal is limited, the aim of the present study was to detect bacteria and parasites with veterinary and zoonotic importance in ticks collected from dogs, cats, and field vegetation. a total of 925 ticks, comprising 888 (96.0%) adults, 8 (0.9%) nymphs, and 29 (3.1%) larvae, were collected in 4 geographic areas (districts) of portugal. among those, 620 (67.0%) we ... | 2014 | 24745731 |
| serosurvey of tick-borne pathogens in dogs from urban and rural areas from parana state, brazil. | considering the zoonotic potential of tick-borne disease (tbd) agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of tbd agents and risk factors for exposure in two different canine populations from parana state, southern brazil. a total of 138 dog serum samples from urban (ua) (n=68) and rural (ra) (n=70) areas were tested with commercial elisa rapid test for anaplasma phagocytophilum, ehrlichia canis and borr ... | 2013 | 24252955 |
| canine leishmaniosis caused by leishmania major and leishmania tropica: comparative findings and serology. | infection and clinical disease associated with leishmania major and leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. this study describes dogs infected with these leishmania spp. prevalent in the middle east and north africa, and compares the serological response of dogs infected with leishmania infantum, l. major or l. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (elisa) of each species and to rk39 dipstick. | 2017 | 28285601 |
| first detection of tick-borne pathogens of dogs from malta. | the knowledge about the vector-borne infections in domestic dogs has been increasing worldwide. however no studies have been done on hard tick infestation and tick-borne pathogens (tbps) of dogs present in malta. therefore, a total of 99 dogs was selected and inspected between march and july 2013 in 18 urban and 17 rural areas on the islands of malta and gozo. all ticks were removed from the dogs and identified. blood samples were taken and tested for protozoa (babesia spp. and hepatozoon spp.) ... | 2017 | 28143697 |
| canine tick-borne pathogens in cyprus and a unique canine case of multiple co-infections. | canine tick-borne pathogens such as ehrlichia canis and hepatozoon canis are widespread in the mediterranean basin but have never been reported or investigated in cyprus. we describe herein the presence of canine tick-borne pathogens in three dogs with clinical signs compatible with vector-borne diseases from paphos area of cyprus. molecular and phylogenetic analysis revealed the presence of e. canis, anaplasma platys, h. canis, babesia vogeli and mycoplasma haemocanis in cyprus. one dog co-infe ... | 2017 | 28089651 |
| first molecular detection of tick-borne pathogens in dogs from jiangxi, china. | in this study, blood samples obtained from 162 dogs in jiangxi, china, were employed in molecular screening of canine tick-borne pathogens by pcr and sequencing. babesia spp. gene fragment was detected in 12 (7.41%) dogs. all samples were negative for hepatozoon spp., ehrlichia canis, coxiella spp., borrelia spp., rickettsia spp. and anaplasma platys. species-specific pcr analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by babesia canis vogeli and babesia gibsoni, respe ... | 2017 | 27890889 |
| clinical and laboratory features of canine anaplasma platys infection in 32 naturally infected dogs in the mediterranean basin. | since the first description of anaplasma platys infection (api), the disease has been sporadically reported worldwide. whereas it is considered a subclinical disease in the united states or in australia, severe cases are reported in europe. thus far, little information is available regarding the clinical and laboratory findings associated with the disease and the implication of co-infections with other vector-borne pathogens (vbps) in southern europe. the purpose of the study was to describe cli ... | 2016 | 27426437 |
| detection of ehrlichia canis, babesia vogeli, and toxoplasma gondii dna in the brain of dogs naturally infected with leishmania infantum. | the aims of this study were to investigate the presence of leishmania infantum and possible co-infection with anaplasma platys , babesia vogeli, ehrlichia canis , and toxoplasma gondii in the brain of 24 dogs naturally infected by l. infantum . a total of 24 mongrel adult dogs (22 clinically affected, 2 with neurological signs, and 2 subclinically infected) aged between 2 and 5 yr, naturally infected by visceral leishmaniasis, were selected. fragments from meninges, frontal cortex, thalamus, cer ... | 2016 | 26765523 |
| a survey of tick-borne pathogens in dogs and their ticks in the pantanal biome, brazil. | tick and blood samples collected from domestic dogs in the brazilian pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. among 320 sampled dogs, 3.13% were infected by babesia vogeli (piroplasmida: babesiidae), 8.75% by hepatozoon canis (eucoccidiorida: hepatozoidae), 7.19% by anaplasma platys (rickettsiales: anaplasmataceae), and 0.94% by an unclassified anaplasma sp. in three tick species collected from dogs, the following tick-borne agents were dete ... | 2016 | 26467462 |
| evaluation of blood and bone marrow in selected canine vector-borne diseases. | bone marrow (bm) is a major hematopoietic organ that can harbour a variety of vector-borne pathogens; however, knowledge of bm pathological changes in dogs infected with vector-borne pathogens is limited. thus, the aim of the present study was to assess the pathological changes in canine bm associated with natural infections by four vector-borne pathogens, as well as to determine the relationships between such changes and abnormalities of the peripheral blood. | 2014 | 25441458 |
| high throughput pyrosequencing technology for molecular differential detection of babesia vogeli, hepatozoon canis, ehrlichia canis and anaplasma platys in canine blood samples. | canine babesiosis, hepatozoonosis, ehrlichiosis, and anaplasmosis are tick-borne diseases caused by different hemopathogens. these diseases are causes of morbidity and mortality in dogs. the classic method for parasite detection and differentiation is based on microscopic observation of blood smears. the limitations of the microscopic method are that its performance requires a specially qualified person with professional competence, and it is ineffective in differentiating closely related specie ... | 2014 | 24704311 |
| molecular and serological detection of tick-borne pathogens in dogs from an area endemic for leishmania infantum in mato grosso do sul, brazil. | tick-borne pathogens affect a wide range of vertebrate hosts. to identify tick-borne pathogens among dogs from campo grande, ms, brazil testing seropositive for leishmania infantum (syn. l. chagasi), a serological and molecular study was conducted to detect ehrlichia canis, anaplasma platys and babesia vogeli in 60 serum and spleen samples. a confirmatory diagnosis of l. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to ... | 2014 | 24473877 |
| vector-borne pathogens in dogs from costa rica: first molecular description of babesia vogeli and hepatozoon canis infections with a high prevalence of monocytic ehrlichiosis and the manifestations of co-infection. | infection with canine vector-borne pathogens was evaluated in dogs from four different regions of costa rica by pcr. demographic data, clinical signs, packed cell volume values, and the presence of tick infestation were recorded for each dog. forty seven percent (69/146) of the dogs were infected with at least one pathogen and 12% were co-infected with two pathogens. ehrlichia canis was detected in 34%, anaplasma platys in 10%, babesia vogeli in 8%, and hepatozoon canis in 7.5% of the blood samp ... | 2014 | 24315693 |
| babesia canis vogeli, ehrlichia canis, and anaplasma platys infection in a dog. | a 12-month-old male neutered mixed breed dog was presented with a history of diarrhea, lethargy, emaciation, polydypsia, and sniffling. physical examination findings included pale mucous membranes, increased heart and respiratory rates, and normal rectal temperature (38°c). hematologic abnormalities included anemia and thrombocytopenia. biochemical abnormalities included hypoalbuminemia, hyperbilirubinemia, and elevated alp and alt activities. a snap 4dx test result was positive for ehrlichia ca ... | 2013 | 24138512 |
| tick-borne pathogens and disease in dogs on st. kitts, west indies. | between 2009 and 2011, we conducted a case-control study of ticks and tick-associated pathogens affecting dogs on the island of st. kitts, eastern caribbean, including 55 cases of clinically suspected tick-borne disease (tbd) and 110 presumably healthy animals presented for elective surgeries. rhipicephalus sanguineus caused year-round infestations of dogs, and 36% of the dogs in the study were infested at the time of examination. overall, 62% of suspected tbd cases and 24% of presumably healthy ... | 2013 | 23481028 |
| tick-borne agents in domesticated and stray cats from the city of campo grande, state of mato grosso do sul, midwestern brazil. | anaplasmataceae agents, piroplasmids and hepatozoon spp. have emerged as important pathogens among domestic and wild felines. the present work aimed to detect the presence of species belonging to the anaplasmataceae family, piroplasmas and hepatozoon spp. dna in blood samples of domesticated and stray cats in the city of campo grande, state of mato grosso do sul, midwestern brazil. between january and april 2013, whole blood samples were collected from 151 cats (54 males, 95 females and two with ... | 2015 | 26187416 |
| black-backed jackals (canis mesomelas) are natural hosts of babesia rossi, the virulent causative agent of canine babesiosis in sub-saharan africa. | babesia rossi, which is transmitted by haemaphysalis spp. and is highly virulent to domestic dogs, occurs only in sub-saharan africa. since dogs are not native to the region, it has been postulated that the natural host of b. rossi is an indigenous african canid. although various attempts at artificial infection indicated that black-backed jackals (canis mesomelas) could become subclinically infected with b. rossi, data on occurrence of b. rossi in free-ranging jackals was lacking. a long-term b ... | 2017 | 28285591 |
| mitochondrial genome sequences and structures aid in the resolution of piroplasmida phylogeny. | the taxonomy of the order piroplasmida, which includes a number of clinically and economically relevant organisms, is a hotly debated topic amongst parasitologists. three genera (babesia, theileria, and cytauxzoon) are recognized based on parasite life cycle characteristics, but molecular phylogenetic analyses of 18s sequences have suggested the presence of five or more distinct piroplasmida lineages. despite these important advancements, a few studies have been unable to define the taxonomic re ... | 2016 | 27832128 |
| withdrawn:first report on babesia canis vogeli infection in dogs in the philippines. | the publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.doi.org/10.1016/j.parint.2016.10.001.the duplicate article has therefore been withdrawn. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2016 | 27815229 |
| confirmation of occurrence of <i>babesia vogeli</i> in a dog in windhoek, central namibia. | although there is evidence of high seroprevalence of antibodies to babesia spp. in dogs in central namibia, clinical babesiosis is rarely diagnosed. rhipicephalus sanguineus sensu lato, the vector of babesia vogeli, is common in namibia while haemaphysalis elliptica, the vector of the highly virulent but morphologically indistinguishable babesia rossi, has rarely been recorded, mainly in northern namibia. on the basis of vector occurrence, clinical cases of canine babesiosis in windhoek, central ... | 2016 | 27796110 |
| development of recombinant bgp12 based enzyme linked immunosorbent assays for serodiagnosis of babesia gibsoni infection in dogs. | indirect elisa and dot-elisa using recombinant bgp12 (rbgp12) were developed for the diagnosis of babesia gibsoni infected dogs. the complete open reading frame of bgp12 gene (378bp) was cloned in pet-32a(+) expression vector and expressed in escherichia coli as a soluble thioredoxin (trx) fusion protein. the purified rbgp12 was used for production of anti-rbgp12 rabbit serum, which recognized a native 12-kda protein in b. gibsoni infected erythrocyte by western blot analysis. to evaluate the po ... | 2016 | 26827835 |
| vector-borne diseases of small companion animals in namibia: literature review, knowledge gaps and opportunity for a one health approach. | namibia has a rich history in veterinary health but little is known about the vector-borne diseases that affect companion dogs and cats. the aim of this review is to summarise the existing published and available unpublished literature, put it into a wider geographical context, and explore some significant knowledge gaps. to date, only two filarial pathogens (dirofilaria repens and acanthocheilonema dracunculoides) and three tick-borne pathogens (babesia canis vogeli, hepatozoon canis and ehrlic ... | 2015 | 26824340 |
| molecular detection of ehrlichia canis, hepatozoon canis and babesia canis vogeli in stray dogs in mahasarakham province, thailand. | canine tick borne diseases showing distribution worldwide have caused morbidity and mortality in dogs. this study observed the mainly tick borne pathogens described for dogs in thailand, ehrlichia canis, hepatozoon canis and babesia canis vogeli. from may to july 2014, blood samples were collected from 79 stray dogs from 7 districts of mahasarakham province to molecular surveyed for 16s rrna gene of e. canis and 18s rrna gene of h. canis and b. canis vogeli. twenty eight (35.44%) of stray dogs s ... | 2015 | 26568991 |
| leishmania, babesia and ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods? | the present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-leishmania spp., -babesia canis vogeli and -ehrlichia canis antibodies in urban dogs from an area endemic to these parasites. | 2015 | 25860466 |
| detection of babesia canis vogeli and hepatozoon canis in canine blood by a single-tube real-time fluorescence resonance energy transfer polymerase chain reaction assay and melting curve analysis. | a real-time fluorescence resonance energy transfer polymerase chain reaction (qfret pcr) coupled with melting curve analysis was developed for detection of babesia canis vogeli and hepatozoon canis infections in canine blood samples in a single tube assay. the target of the assay was a region within the 18s ribosomal rna gene amplified in either species by a single pair of primers. following amplification from the dna of infected dog blood, a fluorescence melting curve analysis was done. the 2 s ... | 2015 | 25776544 |