| human rhinovirus 87 identified as human enterovirus 68 by vp4-based molecular diagnosis. | human rhinoviruses (hrvs) are the major cause of respiratory infections. we developed a diagnostic method for hrvs based on the reverse-transcription polymerase chain reaction (rt-pcr) and vp4-based phylogenetic analysis. a set of primers used in the rt-pcr of human enteroviruses (evs) appeared to be capable of amplifying all prototype strains of hrvs, each of which generated a 530-bp fragment. the single exception was hrv-87, which generated a 650-bp fragment, as observed in human evs. the vp4 ... | 2002 | 12403917 |
| human rhinovirus 87 and enterovirus 68 represent a unique serotype with rhinovirus and enterovirus features. | it has recently been reported that all but one of the 102 known serotypes of the genus rhinovirus segregate into two genetic clusters (c. savolainen, s. blomqvist, m. n. mulders, and t. hovi, j. gen. virol. 83:333-340, 2002). the only exception is human rhinovirus 87 (hrv87). here we demonstrate that hrv87 is genetically and antigenically highly similar to enterovirus 68 (ev68) and is related to ev70, the other member of human enterovirus group d. the partial nucleotide sequences of the 5' untra ... | 2002 | 12409401 |