| molecular characterization and coat protein serology of watermelon leaf mottle virus (potyvirus). | a cdna library was generated from purified rna of watermelon leaf mottle virus (wlmv) (genus potyvirus). two overlapping clones totaling 2,316 nucleotides at the 3' terminus of the virus were identified by immunoscreening with coat protein antiserum. the sequence analyses of the clones indicated an open reading frame (orf) of 2,050 nucleotides which encoded part of the replicase and the coat protein, a 243-nucleotide non-coding region (3'utr), and 23 adenine residues of the poly (a) tail. the ta ... | 2000 | 10795529 |
| on the evolution and molecular epidemiology of the potyvirus papaya ringspot virus. | the potyvirus papaya ringspot virus (prsv) is found throughout the tropics and subtropics. its p biotype is a devastating pathogen of papaya crops and its w biotype of cucurbits. prsv-p is thought to arise by mutation from prsv-w. however, the relative impact of mutation and movement on the structure of prsv populations is not well characterized. to investigate this, we have determined the coat protein sequences of isolates of both biotypes of prsv from vietnam (50), thailand (13), india (1) and ... | 2002 | 12237441 |
| molecular evidence that zucchini yellow fleck virus is a distinct and variable potyvirus related to papaya ringspot virus and moroccan watermelon mosaic virus. | zucchini yellow fleck virus (zyfv, genus potyvirus) infects cultivated or wild cucurbits in the mediterranean basin and occasionally causes severe damage in crops. biological and serological data tend to indicate that zyfv is related to other cucurbit-infecting potyviruses, mainly papaya ringspot virus (prsv) and moroccan watermelon mosaic virus (mwmv). in order to establish unambiguously the taxonomic status of zyfv, the sequence of the 3' part of the genome - encompassing the cp coding region ... | 2007 | 16969629 |
| biological characterization and complete nucleotide sequence of a tunisian isolate of moroccan watermelon mosaic virus. | during a survey conducted in october 2005, cucurbit leaf samples showing virus-like symptoms were collected from the major cucurbit-growing areas in tunisia. das-elisa showed the presence of moroccan watermelon mosaic virus (mwmv, potyvirus), detected for the first time in tunisia, in samples from the region of cap bon (northern tunisia). mwmv isolate tn05-76 (mwmv-tn) was characterized biologically and its full-length genome sequence was established. mwmv-tn was found to have biological propert ... | 2008 | 17978887 |
| algerian watermelon mosaic virus (awmv): a new potyvirus species in the prsv cluster. | a potyvirus was isolated from a naturally infected squash plant in algeria in 1986. biological and serological data have revealed that the virus, initially described as h4, is related to other cucurbit-infecting potyviruses, particularly moroccan watermelon mosaic virus (mwmv) and papaya ringspot virus (prsv). to establish unequivocally the taxonomic status of h4, its full-length genome sequence was established. h4 shared identities of 70% and 65% at the amino acid level with mwmv and prsv, resp ... | 2008 | 18484176 |
| melon rna interference (rnai) lines silenced for cm-eif4e show broad virus resistance. | efficient and sustainable control of plant viruses may be achieved using genetically resistant crop varieties, although resistance genes are not always available for each pathogen; in this regard, the identification of new genes that are able to confer broad-spectrum and durable resistance is highly desirable. recently, the cloning and characterization of recessive resistance genes from different plant species has pointed towards eukaryotic translation initiation factors (eif) of the 4e family a ... | 2012 | 22309030 |
| genome sequence analysis of two south african isolates of moroccan watermelon mosaic virus infecting cucurbits. | moroccan watermelon mosaic virus (mwmv) has been prevalent in cucurbits in the republic of south africa (rsa) since it was first reported in 1987. however, full genome studies of the south african isolates have never been conducted previously. the full genome of two mwmv isolates infecting cucurbits (cucurbita pepo l.) in the province of kwazulu-natal, rsa, was compared with the genome of the tunisian isolate in this communication. the genome sequences of the rsa mwmv isolates were elucidated us ... | 2016 | 27448681 |
| zucchini shoestring virus: a distinct potyvirus in the papaya ringspot virus cluster. | zucchini shoestring virus (zssv) has been proposed to be a putative potyvirus in the papaya ringspot virus (prsv) cluster, based on the sequence similarity of its coat protein to those of related potyviruses. zssv has been associated with the outbreak of a damaging disease of baby marrow (cucurbita pepo l.) that had been observed throughout the province of kwazulu-natal, in the republic of south africa (rsa). we report the genome sequence of zssv, determined by next-generation sequencing of tota ... | 2016 | 27216927 |
| new species in the papaya ringspot virus cluster: insights into the evolution of the prsv lineage. | the "papaya ringspot virus (prsv) cluster" of cucurbit-infecting potyviruses contains five acknowledged species that have similar biological, serological and molecular properties. additional data suggest there are other uncharacterized species from various locations in the world that likely belong to the prsv cluster including a new prsv-like virus reported from sudan in 2003. molecular and biological data indicated that the virus from sudan belongs to a new species, tentatively named wild melon ... | 2017 | 28669763 |