development of nested polymerase chain reaction detection of mycosphaerella spp. and its application to the study of leaf disease in eucalyptus plantations. | abstract mycosphaerella leaf disease (mld) is a serious disease of two of the major eucalypt species grown in temperate regions worldwide, eucalyptus globulus and e. nitens. more than 30 species of mycosphaerella have been reported on eucalypts worldwide. accurate, rapid, and early discrimination of mycosphaerella spp. causing crown damage to e. globulus and e. nitens will assist the development of sustainable management strategies. this study describes the development, and incorporation in a ne ... | 2007 | 18944368 |