| [genetic-physiological compatibility and karyotypes of stem nematode]. | the paper presents literary and the authors own data. the potato stem eelworm ditylenchus destructor has a set of chromosomes 2n=44-48 and does not cross with the close species d. dipsaci from onion, garlic, parsley and parsnip. most stem eelworms of the collective species d. dipsaci have n=12 and cross with each other producing fertile progeny. some forms of stem eelworms, e.g. those of broad beans, plantain, dandelion and falcaria are polyploid. intraspecies and intrapopulation polymorphism ac ... | 1976 | 1026912 |
| identification and analysis of a cuticular collagen-encoding gene from the plant-parasitic nematode meloidogyne incognita. | the vast majority of proteins in the nematode cuticle are collagens. cuticular collagen-encoding genes (col) have been described for the animal parasites ascaris suum and haemonchus contortus and for the free-living caenorhabditis elegans. the proteins encoded by all these genes seem to have the same basic structure, indicating that there is a conserved subfamily of cuticular col in these nematodes. in this paper, we describe the identification and characterization of a cdna (lemmi 5) which corr ... | 1994 | 7828882 |
| occurrence of plant parasitic nematodes (tylenchina) in sugar beet fields in fars province, iran. | on a survey of plant parasitic nematode fauna, belonging to the suborder tylenchina, about 110 soil and root samples were collected from different sugar beet field in fars province in iran. the samples were washed and the nematodes were extracted by centrifugal flotation technique according to the modified method by de grisse, 1969. they were fixed and transferred to glycerine, then permanent slides were mounted. morphological and morphometrical characters of collected species were carefully stu ... | 2004 | 15759441 |
| response of pinus ponderosa seedlings to stylet-bearing nematodes. | of 12 stylet-bearing nematodes used for inoculations, pratylenchus penetrans, p. brachyurus, p. vulnus, ditylenchus destructor, meloidogyne incognita, m. javanica, and m. hapla reproduced on pinus ponderosa, while xiphinema index, aphelenchus avenae, paratylenehus neoamblycephalus, tylenchulus semipenetrans, and macroposthonia xenoplax did not. p. vulnus, p. brachyurus, p. penetrans, a. avenae, d. destructor, t. semipenetrans, and p. neoamblycephalus significantly suppressed both the shoot and r ... | 1979 | 19300659 |
| [cloning and sequence analysis of a new cathepsin l-like cysteine proteinase gene from ditylenchus destructor]. | the cathepsin l-like cysteine proteinase genes (cpls) are multifunction genes related to the parasitic abilities of plant parasitic nematodes. a new cathepsin l-like cysteine proteinase gene (dd-cpl-1) (genbank accession gq 180107) was cloned from ditylenchus destructor by rt-pcr and race. the cdna sequence consisted of a 1 131 bp open reading frame (orf) encoding 376 amino acid residues that were franked by a 29 bp 5'-untranslated region (utr) and a 159 bp 3'-utr. genomic sequence analysis show ... | 2011 | 21553491 |
| the purl gene of bacillus subtilis is associated with nematicidal activity. | parasitic nematodes of plants are important plant pathogens that represent a significant financial burden on agriculture. this study evaluated the efficacy of bacillus spp. as nematode biocontrol agents and identified bacillus genes associated with nematicidal activity. culture by products of bacillus subtilis strains okb105 and 69 and bacillus amyloliquefaciens strains fzb42 and b3 were used to treat aphelenchoides besseyi, ditylenchus destructor, bursaphelenchus xylophilus and meloidogyne java ... | 2011 | 21671997 |
| proteomic profile of the bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole. | benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against sclerotinia sclerotiorum, ditylenchus destructor and bradysia odoriphaga. however, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. here, we employed itraq-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of b. odoriphaga. in response to benzothiazole, 92 of ... | 2016 | 27883048 |
| isolation and identification of nematode-antagonistic compounds from the fungus aspergillus candidus. | culture medium from an isolate of the fungus aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. column, thin layer and preparative chromatographies and spectral and elemental analyses, were used to isolate and identify two major constituents of an active fraction (fraction f) obtained from the medium. compound 1 was identified as 2-hydroxypropane-1, 2, 3-tricarboxylic ac ... | 2016 | 26850440 |
| isolation and identification of chemical constituents from the bacterium bacillus sp. and their nematicidal activities. | a strain smrs28 was isolated from the rhizosphere soil of a toxic plant stellera chamaejasme and identified as bacillus sp. on the basis of morphological and partial 16s rrna gene sequence analysis. the crude extract of smrs28 fermentation broth showed strong nematocidal activities in preliminary test. to define the active nematocidal metabolites of smrs28, a novel compound (1), 4-oxabicyclo[3.2.2]nona-1(7), 5,8-triene, along with five known compounds (2-6), were isolated from the strain by vari ... | 2015 | 26058971 |
| expression and evolutionary analyses of three acetylcholinesterase genes (mi-ace-1, mi-ace-2, mi-ace-3) in the root-knot nematode meloidogyne incognita. | the full cdna of mi-ace-3 encoding an acetylcholinesterase (ache) in meloidogyne incognita was cloned and characterized. mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. key residues essential to ache structure and function were conserved. the deduced mi-ace-3 protein sequence had 72% amino acid similarity with that of ditylenchus destructor dd-ache-3. phylogenetic analyses using 41 aches from 24 species showed that mi-ace-3 formed a cluster with 4 other nematode a ... | 2017 | 28238686 |
| the ditylenchus destructor genome provides new insights into the evolution of plant parasitic nematodes. | plant-parasitic nematodes were found in 4 of the 12 clades of phylum nematoda. these nematodes in different clades may have originated independently from their free-living fungivorous ancestors. however, the exact evolutionary process of these parasites is unclear. here, we sequenced the genome sequence of a migratory plant nematode, ditylenchus destructor we performed comparative genomics among the free-living nematode, caenorhabditis elegans and all the plant nematodes with genome sequences av ... | 2016 | 27466450 |
| paraphyletic genus ditylenchus filipjev (nematoda, tylenchida), corresponding to the d. triformis-group and the d. dipsaci-group scheme. | the genus ditylenchus has been divided into 2 groups: the ditylenchus triformis-group, and the ditylenchus dipsaci-group based on morphological and biological characters. a total of 18 populations belong to 5 species of ditylenchus was studied: ditylenchus africanus, ditylenchus destructor, ditylenchus myceliophagus and dipsaci, ditylenchus weischeri, the first 3 belong to the ditylenchus triformis-group, the last 2 the ditylenchus dipsaci-group. the species of ditylenchus triformis-group were c ... | 2016 | 27103870 |
| resistance to ditylenchus destructor infection in sweet potato by the expression of small interfering rnas targeting unc-15, a movement-related gene. | stem nematode (ditylenchus destructor) is one of most serious diseases that limit the productivity and quality of sweet potato (ipomoea batatas), a root crop with worldwide importance for food security and nutrition improvement. hence, there is a global demand for developing sweet potato varieties that are resistant to the disease. in this study, we have investigated the interference of stem nematode infectivity by the expression of small interfering rnas (sirnas) in transgenic sweet potato that ... | 2015 | 26034810 |
| a fast and sensitive method for the simultaneous identification of three important nematode species of the genus ditylenchus. | nematodes of the genus ditylenchus are parasites of a wide range of hosts, including higher plants. the most destructive of these species are d. dipsaci and d. destructor, two frequently quarantined pests. no rapid molecular method is available for unambiguous detection and distinguishing of these species from each other or from d. gigas, a pest of vicia faba, either by multiplex pcr or real-time pcr. | 2015 | 24687757 |
| exploring the host parasitism of the migratory plant-parasitic nematode ditylenchus destuctor by expressed sequence tags analysis. | the potato rot nematode, ditylenchus destructor, is a very destructive nematode pest on many agriculturally important crops worldwide, but the molecular characterization of its parasitism of plant has been limited. the effectors involved in nematode parasitism of plant for several sedentary endo-parasitic nematodes such as heterodera glycines, globodera rostochiensis and meloidogyne incognita have been identified and extensively studied over the past two decades. ditylenchus destructor, as a mig ... | 2013 | 23922743 |
| [toxicity and bioactivity of several alternative nematocides against ditylenchus destructor]. | by the methods of fumigation and contact assay, this paper determined the toxicity of four kinds of soil fumigants and six kinds of non-fumigants against ditylenchus destructor. in the meantime, the bioactivity of the agents was evaluated by the corrected mortality rate of d. destructor after treated with the lc50 dose of each agent for different time. the lc50 values of soil fumigants dazomet, 1, 3-dichloropropene, metham-sodium, and chloropicrin were 0.49, 0.89, 0.91, and 3.60 mg x l(-1), and ... | 2011 | 22303683 |
| [molecular cloning and characterization of an acetylcholinesterase gene dd-ace-2 from sweet potato stem nematode ditylenchus destructor]. | a cdna, named dd-ace-2, encoding an acetylcholinesterase (ache, ec3.1.1.7), was isolated from sweet-potato-stem nematode, ditylenchus destructor. the nucleotide and amino acid sequences among different nematode species were compared and analyzed with dnaman5.0, mega3.0 softwares. the results showed that the complete nucleotide sequence of dd-ace-2 gene of ditylenchus destructor contains 2425 base pairs from which deduced 734 amino acids (genbank accession no. ef583058). the homology rates of ami ... | 2008 | 18464607 |
| separation of three species of ditylenchus and some host races of d. dipsaci by restriction fragment length polymorphism. | this study examined the ribosomal cistron of ditylenchus destructor, d. myceliophagus and seven host races of d. dipsaci from different geographic locations. the three species showed restriction fragment length polymorphisms (rflps) in the ribosomal cistron, the 18s rdna gene, and the ribosomal internal transcribed spacer (its). southern blot analysis with a 7.5-kb ribosomal cistron probe differentiated the five host races of d. dipsaci examined. polymerase chain reaction (pcr) amplification of ... | 1993 | 19279809 |
| reproductive and damage potential of ditylenchus destructor on six peanut cultivars. | commercial peanut cultivars were evaluated for host suitability and sensitivity to ditylenchus destructor. all cultivars were susceptible. approximately 94% of the final population were in the pods. highest pf occurred at harvest on early maturing cultivars. damage occurred on four of six cuttivars at pi = 100/3 liters of soil and all six cultivars at pi = 1,000. norden and selmani were the most susceptible cultivars. sellie was the most tolerant and highest yielding cultivar. this cultivar may ... | 1993 | 19279743 |
| minimizing damage by ditylenchus destructor to peanut seed with early harvest. | greenhouse and microplot experiments were conducted to evaluate the damage potential of ditylenchus destructor on four south african commercial peanut cultivars as influenced by harvest date. the cultivars sellie and harts should be harvested by 150 and 120 days after planting, respectively. losses were 12-13% with early harvest, but a 15-day delay resulted in losses of 45-49%. harvest of natal common and norden at 125 and 145 days after planting, respectively, resulted in the highest seed grade ... | 1992 | 19283031 |
| effect of host plant age on population development and pathogenicity of ditylenchus destructor on peanut. | the effect of inoculating peanut, arachis hypogaea cv. sellie, with ditylenchus destructor at timed intervals after planting and with different initial nematode population densities (pi) was tested in greenhouse experiments. final nematode population densities (pf) in hulls and seeds were greater (pf < 0.001) in plants inoculated at or before 9 weeks after planting. pod disease symptoms correlated positively with the pf in the pods. the seedgrade of peanuts inoculated at or before 9 weeks after ... | 1992 | 19283001 |
| population dynamics of ditylenchus destructor on peanut. | the population development of ditylenchus destructor in the roots, pegs, hulls, and seeds of eight peanut (arachis hypogaea) genotypes was studied in the greenhouse. although all genotypes tested were good hosts for d. destructor, differences in host suitability were observed. invasion of the plant parts by ditylenchus destructor proceeded more slowly in genotypes with long growth periods. during the second half of the growth period of these genotypes, d. destructor populations emigrated from th ... | 1991 | 19283159 |
| reproductive and damage potential of ditylenchus destructor on peanut. | the reproductive and damage potential of ditylenchus destructor on peanut, arachis hypogaea cv. sellie, was determined in greenhouse tests. final nematode population densities (pf) in roots, hulls, and seeds increased (p = 0.01) as a function of increasing initial population (pi). final population densities were higher in hulls than in seeds and roots. final densities in hulls and seeds were positively (p = 0.01) correlated. fresh root and hull weight and number of pods and seeds per plant were ... | 1991 | 19283089 |
| in vitro embryo explant cultures of peanut to evaluate resistance to ditylenchus destructor. | population densities of d. destructor on embryo explants of 22 peanut genotypes grown in vitro were compared with those in roots and seeds of the same genotypes grown in the greenhouse. during the first 8 weeks after inoculation, the optimum incubation period was 6 weeks for maximum reproduction of ditylenchus destructor on embryo explants of peanut (arachis hypogaea l. cv. sellie) inoculated with 250 nematodes at 25 c. nematode numbers increased 17-fold. deletion of mnso ho and a higher khpo co ... | 1990 | 19287727 |
| host status of seven weed species and their effects on ditylenchus destructor infestation of peanut. | the host suitability to ditylenchus destructor of seven common weed species in peanut (arachis hypogaea) fields in south africa was determined. based on the number of nematodes per root unit, white goosefoot (chenopodium album), feathertop chloris (chloris virgata), purple nutsedge (cyperus rotundus), jimson weed (datura stramonium), goose grass (eleusine indica), khaki weed (tagetes minuta), and cocklebur (xanthium strumarium) were poor hosts. ditylenchus destructor survived on all weed species ... | 1990 | 19287723 |
| histopathology of ditylenchus destructor on peanut. | the time and mode of entry, and development of ditylenchus destructor in peanut were studied in field and greenhouse experiments. few nematodes were present in the cortex of the roots. at 90-120 days after planting, d. destructor was observed in the exocarp at the base of the pod near the point of connection with the peg. the peg was invaded from this primary infection site. the endocarp of the hull was usually penetrated through openings at the base of the mesocarp and sometimes at the pod apex ... | 1990 | 19287720 |
| ditylenchus destructor in hulls and seeds of peanut. | the potato rot nematode, ditylenchus destructor thorne, is reported for the first time in hulls and seeds of peanut. the populations found differed from d. dipsaei and d. myceliophagus in habitat, number of lateral incisures, shape of tail tip, and length of postvulval sac. infected hulls had brown necrotic tissue at the point of connection with the peg, and a black discoloration appeared first along the longitudinal veins. infected seeds were usually shrunken, and testae and embryos had a yello ... | 1989 | 19287571 |
| efficacy of selected nonvolatile nematicides on control of ditylenchus destructor in iris. | greenhouse and field tests established that fenamiphos at 6.7 and 13.4 kg ai/ha applied in a 30-cm band directly on iris bulbs at planting effectively controlled ditylenchus destructor. aldicarb at rates of 5.6 to 11.2 kg ai/ha was less effective. carbofuran, fensulfothion, and oxamyl at 6.7 to 13.4 kg ai/ha were ineffective. when applied on the bulbs, fenamiphos (granular or liquid) reduced nematode infection from 31 to 0.6% as determined by visual inspection of bulbs at harvest. populations of ... | 1983 | 19295770 |
| [ditylenchus destructor in seed potato imported from holland]. | | 1953 | 13186388 |
| some host relationships of the potato-rot nematode, ditylenchus destructor thorne, 1945. | | 1951 | 14843130 |