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genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting capsicum annuum var. aviculare in mexico.the characterization of viruses infecting wild plants is a key step towards understanding the ecology of plant viruses. in this work, the complete genomic nucleotide sequence of a new tymovirus species infecting chiltepin, the wild ancestor of capsicum annuum pepper crops, in mexico was determined, and its host range has been explored. the genome of 6,517 nucleotides has the three open reading frames described for tymoviruses, putatively encoding an rna-dependent rna polymerase, a movement prote ...201020229325
effects of plant growth promoting bacteria and mycorrhizal on capsicum annuum l. var. aviculare ([dierbach] d'arcy and eshbaugh) germination under stressing abiotic conditions.capsicum annuum var. aviculare to tarahumara and papago indians and farmers of sonora desert is a promising biological and commercial value as a natural resource from arid and semiarid coastal zones. traditionally, apply synthetic fertilizers to compensate for soil nitrogen deficiency. however, indiscriminate use of these fertilizers might increase salinity. the inoculation by plant growth promoting bacteria (pgpb) and arbuscular mycorrhizal fungi (amf) represents an alternative as potential bio ...201020447830
impact of human management on the genetic variation of wild pepper, capsicum annuum var. glabriusculum.management of wild peppers in mexico has occurred for a long time without clear phenotypic signs of domestication. however, pre-domestication management could have implications for the population's genetic richness. to test this hypothesis we analysed 27 wild (w), let standing (ls) and cultivated (c) populations, plus 7 samples from local markets (lm), with nine polymorphic microsatellite markers. two hundred and fifty two alleles were identified, averaging 28 per locus. allele number was higher ...201122163053
genome-wide identification and expression analysis of calcium-dependent protein kinase and its closely related kinase genes in capsicum annuum.as ca2+ sensors and effectors, calcium-dependent protein kinases (cdpks) play important roles in plant growth, development, and response to environmental cues. however, no cdpks have been characterized in capsicum annuum thus far. herein, a genome wide comprehensive analysis of genes encoding cdpks and cdpk-related protein kinases (crks) was performed in pepper, a total of 31 cdpk genes and five closely related kinase genes were identified, which were phylogenetically divided into four distinct ...201526442050
[antifungal effect of phenolic and carotenoids extracts from chiltepin (capsicum annum var. glabriusculum) on alternaria alternata and fusarium oxysporum].the effect of phenolic and carotenoid extracts from chiltepin fruits on mycelial growth and the inhibition of conidial germination of alternaria alternata and fusarium oxysporum were investigated in the present work. phenolic extracts inhibited mycelial growth of a.alternata by 38.46%, and significantly reduced conidial germination on the fifth day after treatment to 92% in relation to control. no significant changes were observed in the inhibition of mycelial growth in fusarium oxysporum; howev ...201525705046
the effect of ecosystem biodiversity on virus genetic diversity depends on virus species: a study of chiltepin-infecting begomoviruses in mexico.current declines in biodiversity put at risk ecosystem services that are fundamental for human welfare. increasing evidence indicates that one such service is the ability to reduce virus emergence. it has been proposed that the reduction of virus emergence occurs at two levels: through a reduction of virus prevalence/transmission and, as a result of these epidemiological changes, through a limitation of virus genetic diversity. although the former mechanism has been studied in a few host-virus i ...201527774278
human management of a wild plant modulates the evolutionary dynamics of a gene determining recessive resistance to virus infection.this work analyses the genetic variation and evolutionary patterns of recessive resistance loci involved in matching-allele (ma) host-pathogen interactions, focusing on the pvr2 resistance gene to potyviruses of the wild pepper capsicum annuum glabriusculum (chiltepin). chiltepin grows in a variety of wild habitats in mexico, and its cultivation in home gardens started about 25 years ago. potyvirus infection of capsicum plants requires the physical interaction of the viral vpg with the pvr2 prod ...201627490800
the complete chloroplast genome of capsicum annuum var. glabriusculum using illumina sequencing.chloroplast (cp) genome sequences provide a valuable source for dna barcoding. molecular phylogenetic studies have concentrated on dna sequencing of conserved gene loci. however, this approach is time consuming and more difficult to implement when gene organization differs among species. here we report the complete re-sequencing of the cp genome of capsicum pepper (capsicum annuum var. glabriusculum) using the illumina platform. the total length of the cp genome is 156,817 bp with a 37.7% overal ...201526205052
the complete chloroplast genome sequence of american bird pepper (capsicum annuum var. glabriusculum).the complete chloroplast genome sequence of american bird pepper (capsicum annuum var. glabriusculum) is reported and characterized in this study. the genome size is 156,612 bp, containing a pair of inverted repeats (irs) of 25,776 bp separated by a large single-copy region of 87,213 bp and a small single-copy region of 17,851 bp. the chloroplast genome harbors 130 known genes, including 89 protein-coding genes, 8 ribosomal rna genes, and 37 trna genes. a total of 18 of these genes are duplicate ...201624810071
characterization of different capsicum varieties by evaluation of their capsaicinoids content by high performance liquid chromatography, determination of pungency and effect of high temperature.the chili pepper is a very important plant used worldwide as a vegetable, as a spice, and as an external medicine. in this work, eight different varieties of capsicum annuum l. have been characterized by their capsaicinoids content. the chili pepper fruits were cultivated in the comarca lagunera region in north of mexico. the qualitative and quantitative determination of the major and minor capsaicinoids; alkaloids responsible for the pungency level, has been performed by a validated chromatogra ...201324184818
landscape heterogeneity shapes host-parasite interactions and results in apparent plant-virus codivergence.knowledge on how landscape heterogeneity shapes host-parasite interactions is central to understand the emergence, dynamics and evolution of infectious diseases. however, this is an underexplored subject, particularly for plant-virus systems. here, we analyse how landscape heterogeneity influences the prevalence, spatial genetic structure, and temporal dynamics of pepper golden mosaic and pepper huasteco yellow vein begomoviruses infecting populations of the wild pepper capsicum annuum glabriusc ...201323379795
characterization of a novel tymovirus on tomato plants in brazil.a tymovirus was isolated in brazil from tomato plants with severe symptoms of leaf mosaic and blistering. the virus was mechanically transmissible to solanaceous indicator host species. the infected plants contained icosahedral particles and chloroplasts with membrane deformations which are typical cytopathic effects caused by tymoviruses. its coat protein amino acid sequence shares the maximum of 64 % identity with the tymovirus chiltepin yellow mosaic virus, which suggested that it can be cons ...201323054432
environmental heterogeneity and the evolution of plant-virus interactions: viruses in wild pepper populations.understanding host-pathogen interactions requires analyses to address the multiplicity of scales in heterogeneous landscapes. anthropogenic influence on plant communities, especially cultivation, is a major cause of environmental heterogeneity. we have approached the analysis of how environmental heterogeneity determines plant-virus interactions by studying virus infection in a wild plant currently undergoing incipient domestication, the wild pepper or chiltepin, across its geographical range in ...201728554561
dramatic number variation of r genes in solanaceae species accounted for by a few r gene subfamilies.most disease resistance genes encode nucleotide-binding-site (nbs) and leucine-rich-repeat (lrr) domains, and the nbs-lrr encoding genes are often referred to as r genes. using newly developed approach, 478, 485, 1,194, 1,665, 2,042 and 374 r genes were identified from the genomes of tomato heinz1706, wild tomato la716, potato dm1-3, pepper zunla-1 and wild pepper chiltepin and tobacco tn90, respectively. the majority of r genes from solanaceae were grouped into 87 subfamilies, including 16 tir- ...201626849045
whole-genome sequencing of cultivated and wild peppers provides insights into capsicum domestication and specialization.as an economic crop, pepper satisfies people's spicy taste and has medicinal uses worldwide. to gain a better understanding of capsicum evolution, domestication, and specialization, we present here the genome sequence of the cultivated pepper zunla-1 (c. annuum l.) and its wild progenitor chiltepin (c. annuum var. glabriusculum). we estimate that the pepper genome expanded ∼0.3 mya (with respect to the genome of other solanaceae) by a rapid amplification of retrotransposons elements, resulting i ...201424591624
effect of biodiversity changes in disease risk: exploring disease emergence in a plant-virus system.the effect of biodiversity on the ability of parasites to infect their host and cause disease (i.e. disease risk) is a major question in pathology, which is central to understand the emergence of infectious diseases, and to develop strategies for their management. two hypotheses, which can be considered as extremes of a continuum, relate biodiversity to disease risk: one states that biodiversity is positively correlated with disease risk (amplification effect), and the second predicts a negative ...201222792068
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