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vulcanisaeta distributa gen. nov., sp. nov., and vulcanisaeta souniana sp. nov., novel hyperthermophilic, rod-shaped crenarchaeotes isolated from hot springs in japan.seventeen strains of rod-shaped, heterotrophic, anaerobic, hyperthermophilic crenarchaeotes were isolated from several hot spring areas in eastern japan, and eight representative strains were characterized further. cells of these strains were straight to slightly curved rods, 0.4-0.6 microm in width. occasionally, cells were branched or bore spherical bodies at the poles. they grew optimally at 85-90 degrees c and at ph 4.0-4.5. they utilized yeast extract, peptone, beef extract, casamino acids, ...200212148613
distribution of 16s rrna introns among the family thermoproteaceae and their evolutionary implications.novel 16s rrna introns were detected in four new strains within the family thermoproteaceae. pyrobaculum oguniense te7(t) and thermoproteus sp. ic-062 housed introns of 32 and 665-668 bp after positions 1205 and 1213 ( escherichia coli numbering system), respectively. caldivirga maquilingensis ic-167(t) had two introns of 37 and 140 bp after positions 901 and 908, respectively. vulcanisaeta distributa ic-065 had a 691-bp intron after position 1391. all the introns larger than 650 bp encoded the ...200312768454
cellular polyamines of the acidophilic, thermophilic and thermoacidophilic archaebacteria, acidilobus, ferroplasma, pyrobaculum, pyrococcus, staphylothermus, thermococcus, thermodiscus and vulcanisaeta.cellular polyamines of newly isolated acidophilic, thermophilic and thermoacidophilic archaebacteria were investigated for the chemotaxonomic significance of polyamine distribution profiles. in addition to spermidine, spermine and agmatine, a quaternary branched penta-amine, n(4)-bis(aminopropyl)spermidine, was found in thermophilic thermococcus waiotapuensis, thermococcus aegaeus and pyrococcus glycovorans belonging to the order thermococcales. an acidophilic euryarchaeon, ferroplasma acidiphil ...200314673752
discovery of a minimal form of rnase p in pyrobaculum.rnase p rna is an ancient, nearly universal feature of life. as part of the ribonucleoprotein rnase p complex, the rna component catalyzes essential removal of 5' leaders in pre-trnas. in 2004, li and altman computationally identified the rnase p rna gene in all but three sequenced microbes: nanoarchaeum equitans, pyrobaculum aerophilum, and aquifex aeolicus (all hyperthermophiles) [li y, altman s (2004) rna 10:1533-1540]. a recent study concluded that n. equitans does not have or require rnase ...201021135215
complete genome sequence of vulcanisaeta distributa type strain (ic-017).vulcanisaeta distributa itoh et al. 2002 belongs to the family thermoproteaceae in the phylum crenarchaeota. the genus vulcanisaeta is characterized by a global distribution in hot and acidic springs. this is the first genome sequence from a member of the genus vulcanisaeta and seventh genome sequence in the family thermoproteaceae. the 2,374,137 bp long genome with its 2,544 protein-coding and 49 rna genes is a part of the genomic encyclopedia of bacteriaand archaea project.201021304741
complete genome sequence of "vulcanisaeta moutnovskia" strain 768-28, a novel member of the hyperthermophilic crenarchaeal genus vulcanisaeta.strain 768-28 was isolated from a hot spring in kamchatka, russia, and represents a novel member of the vulcanisaeta genus. the complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the embden-meyerhof and entner-doudoroff pathways for glucose metabolism, the tricarboxylic acid cycle, beta-oxidation of fatty acids, and sulfate reduction.201121398550
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