| ciguatera incidence and fish toxicity in okinawa, japan. | okinawa being located in the subtropical region has the highest incidence of ciguatera in japan. officially, 33 outbreaks involving 103 patients have been reported between 1997 and 2006. the implicated species were variola louti, lutjanus bohar, lutjanus monostigma, epinephelus fuscoguttatus, unidentified lutjanus sp., plectropomus areolatus, oplegnathus punctatus, epinephelus polyphekadion, caranx ignobilis and moray eel. toxicities of the leftover meals, as determined by mouse bioassays, range ... | 2010 | 19520098 |
| antigenic differences of the scuticociliate miamiensis avidus from japan. | in japan and korea, outbreaks of scuticociliatosis have frequently occurred in japanese flounder, paralichthys olivaceus. morphological observations and small subunit rrna gene sequences have shown that the causative agent of scuticociliatosis in the flounder is miamiensis avidus (syn. philasterides dicentrarchi). in this study, we elucidated the antigenic differences between six japanese m. avidus isolates as an initial step toward developing an effective vaccine against the disease. four japan ... | 2009 | 19702624 |
| a new marine megalocytivirus from spotted knifejaw, oplegnathus punctatus, and its pathogenicity to freshwater mandarinfish, siniperca chuatsi. | megalocytivirus is a newly defined piscine iridovirus and has been shown to be an important causative agent of viral diseases in fish. here, a new megalocytivirus strain, designated skiv-zj07, was isolated from spotted knifejaw (oplegnathus punctatus) using a mandarinfish fry cell line (mff-1). phylogenetic analysis of the major capsid protein and atpase genes showed that skiv-zj07 was most similar to the orange-spotted grouper iridovirus (osgiv) from china and a u1 strain red sea bream iridovir ... | 2010 | 19895861 |
| small subunit ribosomal rna and mitochondrial cytochrome c oxidase subunit 1 gene sequences of 21 strains of the parasitic scuticociliate miamiensis avidus (ciliophora, scuticociliatia). | the scuticociliate miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes. small subunit ribosomal rna (ssu rrna) and mitochondrial cytochrome c oxidase subunit 1 (cox1) genes were analyzed for 21 strains of m. avidus isolated from diseased olive flounder (paralichthys olivaceus), ridged-eye flounder (pleuronichthys cornutus), and spotted knifejaw (oplegnathus fasciatus) in korea and japan (collected in 2003-2007). analysis of ssu rrna gene sequences (1 ... | 2010 | 21113725 |
| sequence conservation in the internal transcribed spacers and 5.8s ribosomal rna of parasitic scuticociliates miamiensis avidus (ciliophora, scuticociliatia). | the scuticociliate miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes, with clinical signs of severe ulcers and hemorrhages in the skeletal muscle. the internal transcribed spacer (its) region, which is widely used in taxonomy and molecular phylogeny because of a high degree of variation, was compared for 21 cloned strains of m. avidus (ciliophora, scuticociliatia). these strains were isolated from olive flounder paralichthys olivaceus, ridged-eye f ... | 2011 | 21362496 |
| detailed lc-ms/ms analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the pacific. | toxin profiles of representative ciguatera species caught at different locations of japan were investigated in fish flesh by high-performance liquid chromatography tandem mass spectrometry (lc-ms/ms) analysis. identification and quantification of 16 toxins were facilitated by the use of 14 reference toxins prepared by either synthesis or isolation from natural sources and the previous lc-ms data thereof. sodium adduct ions [m + na](+) were used as parent and product ions. distinct regional diffe ... | 2011 | 22010820 |
| virions proteins of an rsiv-type megalocytivirus from spotted knifejaw oplegnathus punctatus (skiv-zj07). | megalocytiviruses have three main genotypes, which are represented by isknv, rsiv, and trbiv. to date, the virion-associated proteins of rsiv and trbiv are still unknown. the spotted knifejaw iridovirus (skiv) is a newly characterized rsiv-type megalocytivirus. in this study, the virion-associated proteins of skiv were identified by systemic one-dimensional gel electrophoresis-based proteomic approaches. a total of 49 viral proteins and 33 cellular proteins were associated with the skiv virions ... | 2013 | 23352451 |
| three novel myxobolid species of genera henneguya and myxobolus (myxosporea: bivalvulida) from marine fish in japan. | myxosporean genera henneguya and myxobolus (bivalvulida: myxobolidae) are closely related in morphology and molecular phylogeny, speciose with approximately 1,000 nominal species. the majority of them are recorded from freshwater fish worldwide, and few are known from marine fish. in this study, three myxobolid spp. are described from marine fish around japan. two novel henneguya spp., henneguya ogawai sp. n. and henneguya yokoyamai sp. n., are described from two black sea breams (acanthopagrus ... | 2012 | 22476601 |