| distribution and seasonal variations in levels of three native gnrhs in the brain and pituitary of perciform fish. | specific and sensitive radioimmunoassays (rias) were newly developed for two types of gonadotropin-releasing hormone (gnrh), namely, seabream (sb) gnrh and chicken (c) gnrh-ii. we employed these two rias together with a previously reported ria for salmon (s) gnrh to study the presence and regional distribution of these three gnrhs in the brains and pituitaries of four perciform fishes (red seabream, pagrus major; black seabream, acanthopagrus schlegeli; striped knifejaw, oplegnathus fasciatus; a ... | 1999 | 10201813 |
| complete genomic dna sequence of rock bream iridovirus. | iridovirus is a causative agent of epizootics among cultured rock bream (oplegnathus fasciatus) in korea. here, we report the complete genomic sequence of rock bream iridovirus (rbiv). the genome of rbiv was 112080 bp long and contained at least 118 putative open reading frames (orfs), and its genome organization was similar to that of infectious spleen and kidney necrosis virus (isknv). of the rbiv's 118 orfs, 85 orfs showed 60-99% amino acid identity to those of isknv. phylogenetic analysis of ... | 2004 | 15246274 |
| susceptibility of marine fish species to a megalocytivirus, turbot iridovirus, isolated from turbot, psetta maximus (l.). | turbot iridovirus (tbiv), a member of the genus megalocytivirus in the family iridoviridae, was isolated from diseased turbot, psetta maximus (l.), in korea in 2003. in this study, experimental infection of turbot, japanese flounder, paralichthys olivaceus (temminck & schlegel), and rock bream, oplegnathus fasciatus (temminck & schlegel), with tbiv was performed to evaluate the viral susceptibility of these fish species. after virus exposure, the mortalities of turbot reared at 22 and 25 degrees ... | 2006 | 16866925 |
| expression and serological application of a capsid protein of an iridovirus isolated from rock bream, oplegnathus fasciatus (temminck & schlegel). | iridoviruses infect a wide variety of wild and cultured fish. those iridoviruses belonging to the genus ranavirus, in the iridoviridae family, cause systemic disease in infected animals with a high morbidity and mortality. this paper reports the cloning, sequencing, and expression of the rock bream iridovirus (rbiv) major capsid protein (mcp) in an escherichia coli expression system for subsequent immunological studies. the completeness of the expressed protein was confirmed by peptide mass fing ... | 2007 | 17958613 |
| functional characterization of the rnase iii gene of rock bream iridovirus. | all of the fully sequenced iridoviruses have an orf resembling a putative rnase iii gene. however, to the best of our knowledge, functional characterization of the iridovirus-encoded rnase iii has not been done. in the present study, we have characterized the putative rnase iii of rock bream iridovirus (rbiv), the major cause of mass mortality of cultured rock bream oplegnathus fasciatus in korea. rbiv rnase iii has a single n-terminal endonuclease domain followed by a c-terminal double-stranded ... | 2008 | 18641914 |
| differential modulations of two glyceraldehyde 3-phosphate dehydrogenase mrnas in response to bacterial and viral challenges in a marine teleost oplegnathus fasciatus (perciformes). | glyceraldehyde 3-phosphate dehydrogenase (gapdh) has been recognized as an invariant internal control for various gene expression studies due to its classical housekeeping role in the glycolytic metabolism. however recently, this enzyme has been proven to be a multifunctional protein involved in diverse non-glycolytic activities. in the present study, two paralogue isoforms of gapdh mrnas were characterized from a marine teleost species, rockbream (oplegnathus fasciatus; perciformes) and their t ... | 2008 | 18692141 |
| production of polyclonal antibody against recombinant orf 112 l of rock bream (oplegnathus fasciatus) iridovirus (rbiv) and in vitro neutralization. | rock bream iridovirus (rbiv) is a causative agent of epizootics among cultured rock bream (oplegnathus fasciatus) in korea. in this study, the immunogenic property of ankyrin repeats gene (orf 112l) from rbiv was evaluated to develop vaccines against rbiv. orf 112l of rbiv was cloned into expression vector of pgex-4t-1. the recombinant protein was successfully expressed using e. coli bl21 (de3). the soluble recombinant rbiv protein was applied to affinity column for the purification of the prote ... | 2008 | 19195399 |
| characterization of virus distribution in rock bream (oplegnathus fasciatus; temminck and schlegel) infected with megalocytivirus. | the distribution of virus-infected cells in the organs of rock bream naturally infected with megalocytivirus is reported. examination of sections of liver, spleen and kidney stained by haematoxylin and eosin (he) and periodic acid schiff (pas) revealed the presence of swollen and degenerate cells having morphology consistent with leucocytes. many of these cells were shown to contain viral dna by in-situ hybridization (ish). cells containing viral dna were also found in the connective tissue of o ... | 2009 | 19477463 |
| influence of temperature shifts on the onset and development of red sea bream iridoviral disease in rock bream oplegnathus fasciatus. | the effects of various water temperature treatments on the development of red sea bream iridovirus disease (rsivd) in rock bream oplegnathus fasciatus challenged with iridovirus sachun (ivs-1) were determined by measuring the mortality and the viral concentration in the spleen of infected fish. experimental infections of rock bream with ivs-1 at water temperatures of 18, 21, and 25 degrees c resulted in a cumulative mortality of 100%, but infections at 13 degrees c resulted in 0% mortality, even ... | 2009 | 19565697 |
| heparin cofactor ii (rbhcii) from rock bream (oplegnathus fasciatus): molecular characterization, cloning and expression analysis. | heparin cofactor (hcii) is a serine protease inhibitor (spi), and plays important physiological roles in various biological events including hemostasis. the gene encoding the hcii was isolated from gs-flx™ genomic data of rock bream (oplegnathus fasciatus), designated as rbhcii. the rbhcii (1950 bp) consists of a 1512 bp open reading frame (orf) encoding 504 amino acids (aa), with a signal peptide of 19 aa residues. the predicted molecular mass and the estimated isoelectric point of rbhcii were ... | 2010 | 20951808 |
| galectin-1 (ofglec-1) from rock bream (oplegnathus fasciatus): identification, characterization, gene expression and functional properties. | lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this study, a novel galectin-1 (designated ofglec-1) gene was identified from rock bream, oplegnathus fasciatus, cdna library. the complete cdna sequence of the ofglec-1 gene is 993 base pairs in length, and encodes 135 amino acids. ofglec-1 shares a highly conserved carbohydrate recognition domain with galectin-1 from other fishes. the mrna expressions of ofglec-1 in healthy and bacteri ... | 2010 | 21073891 |
| small subunit ribosomal rna and mitochondrial cytochrome c oxidase subunit 1 gene sequences of 21 strains of the parasitic scuticociliate miamiensis avidus (ciliophora, scuticociliatia). | the scuticociliate miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes. small subunit ribosomal rna (ssu rrna) and mitochondrial cytochrome c oxidase subunit 1 (cox1) genes were analyzed for 21 strains of m. avidus isolated from diseased olive flounder (paralichthys olivaceus), ridged-eye flounder (pleuronichthys cornutus), and spotted knifejaw (oplegnathus fasciatus) in korea and japan (collected in 2003-2007). analysis of ssu rrna gene sequences (1 ... | 2010 | 21113725 |
| characterization and expression analysis of the myeloid differentiation factor 88 (myd88) in rock bream oplegnathus fasciatus. | myeloid differentiation factor 88 (myd88) is a universal adaptor protein able to activate nuclear factor-kappa b (nf-κb) through interactions with interleukin-1 receptor (il-1r) and the toll-like receptors (tlrs), with the exception of tlr3. here, we describe the identification of myd88 from the rock bream fish oplegnathus fasciatus and its characterization based on gs-flx™ sequencing. the cdna of rock bream myd88 was found to be composed of 1626 bp, with an 867 bp open reading frame that encode ... | 2010 | 21152988 |
| characterization and expression analysis of a goose-type lysozyme from the rock bream oplegnathus fasciatus, and antimicrobial activity of its recombinant protein. | lysozyme (muramidase) represents an important defense molecule of the fish innate immune system. known for its bactericidal properties, lysozyme catalyzes the hydrolysis of β-(1,4)-glycosidic bonds between the n-acetyl glucosamine and n-acetyl muramic acid in the peptidoglycan layer of bacterial cell walls. in this study, the complete coding sequence of a g-type lysozyme (rbglyz) was identified in the oplegnathus fasciatus rock bream fish genome by means of multi-tissue normalized cdna pyroseque ... | 2010 | 21167286 |
| molecular characterization and expression analysis of cathepsin b and l cysteine proteases from rock bream (oplegnathus fasciatus). | cathepsins are lysosomal cysteine proteases of the papain family that play an important role in intracellular protein degradation and turn over within the lysosomal system. in the present study, full-length sequences of cathepsin b (rbcathepsin b) and l (rbcathepsin l) were identified after transcriptome sequencing of rock bream oplegnathus fasciatus mixed tissue cdna. cathepsin b was composed of 330 amino acid residues with 36 kda predicted molecular mass. rbcathepsin l contained 336 amino acid ... | 2010 | 21195770 |
| protective effect of herbal and probiotics enriched diet on haematological and immunity status of oplegnathus fasciatus (temminck & schlegel) against edwardsiella tarda. | this study determines the effect of diet enriched with the herb baical skullcap scutellaria baicalensis, and/or probiotics lactobacillus sakei bk19 in rock bream, oplegnathus fasciatus (32 ± 3 g) against edwardsiella tarda. the changes in haematological parameters, innate immune response, and disease resistance were investigated after 1, 3, and 6 weeks. the white blood cell count (wbc: 10(4) mm(-3)), red blood cell count (rbc: 10(6) mm(-3)), and haemoglobin (hb: g dl(-1)) levels significant incr ... | 2011 | 21272648 |
| molecular cloning of rock bream's (oplegnathus fasciatus) tumor necrosis factor receptor-associated factor 2 and its role in nf-?b activiation. | tumor necrosis factor receptor-associated factor 2 (traf2) acts as a transducer of tumor necrosis factor-a (tnf-a)-triggered cell signals which results in inflammation, cell proliferation and antiapoptotic response. in this study, we have cloned cdna of rock bream (oplegnathus fasciatus) traf2, and analyzed its function in activation of nf-?b. the full length cdna of rock bream traf2 consisted of 95 bp 5' utr, 335 bp 3' utr, and 1563 bp orf encoding 520 amino acids that contained n-terminal ring ... | 2011 | 21320605 |
| sequence conservation in the internal transcribed spacers and 5.8s ribosomal rna of parasitic scuticociliates miamiensis avidus (ciliophora, scuticociliatia). | the scuticociliate miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes, with clinical signs of severe ulcers and hemorrhages in the skeletal muscle. the internal transcribed spacer (its) region, which is widely used in taxonomy and molecular phylogeny because of a high degree of variation, was compared for 21 cloned strains of m. avidus (ciliophora, scuticociliatia). these strains were isolated from olive flounder paralichthys olivaceus, ridged-eye f ... | 2011 | 21362496 |
| molecular cloning and expression analysis of a thioredoxin from rock bream, oplegnathus fasciatus, and biological activity of the recombinant protein. | thioredoxins (trxs) are a family of small, highly conserved proteins that are essential for the maintenance of cellular homeostasis. trx1, which contains a conserved redox-active site, cys-gly-pro-cys, is a proinflammatory cytokine, b cell growth factor, macrophage migration inhibiting factor (mif), and an immune regulatory cytokine. the trx1 homologue cdna was isolated from the rock bream lps-stimulated liver cdna library, rbtrx1. rbtrx1 consists of 730 bp full-length cdna with a 324 bp open re ... | 2011 | 21402159 |
| rock bream (oplegnathus fasciatus) serpin, protease nexin-1: transcriptional analysis and characterization of its antiprotease and anticoagulant activities. | protease nexin-1 (pn-1) is a serine protease inhibitor (serpin) protein with functional roles in growth, development, patho-physiology and injury. here, we report our work to clone, analyze the expression profile and characterize the properties of the pn-1 gene in rock bream (rb), oplegnathus fasciatus. rbpn-1 encodes a peptide of 397 amino acids (aa) with a predicted molecular mass of 44 kda and a 23 aa signal peptide. rbpn-1 protein was found to harbor a characteristic serpin domain comprised ... | 2011 | 21419793 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| [characterization of an iridovirus detected in rock bream (oplegnathus fasciatus; temminck and schlegel)]. | during the summer of 2009, mass mortality was observed in cage-cultured rock bream (oplegnathus fasciatus; temminck and schlegel) in the liaoning province. histopathogic studies of the affected fish showed enlarged basophilic cells in the kidney and spleen. these necrotic cells were stained purple using haematoxylin and eosin (he). gf cell cultures showed advanced cytopathic effects after infection with virus supernatants from diseased fish homogenate. transmission electron microscopy revealed h ... | 2011 | 21528541 |
| molecular identification and expression analysis of cathepsins o and s from rock bream, oplegnathus fasciatus. | cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for maintenance of cellular homeostasis. rock bream (oplegnathus fasciatus) cathepsin o and s (rbctso and rbctss, respectively) cdnas were identified by expressed sequence tag (est) analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the full-length rbctso cdna (1698 bp) contained an open reading frame (orf) of 1017 bp encoding 338 am ... | 2011 | 21767649 |
| molecular cloning and expression analysis of two distinct f-type lectins from the rock bream, oplegnathus fasciatus. | several lectin families characterized by distinct signature sequence motifs and structural folds, such as c-type, peptidoglycan recognition protein, ficolin, pentraxins, and most recently galectins, have been implicated in immune surveillance. in this study, two distinct f-type lectins rbftl-1 and rbftl-2, from the rock bream (oplegnathus fasciatus), were identified and their expression was analyzed. the full-length cdna of rbftl-1 was composed of 1204bp with a 945-bp open reading frame (orf) th ... | 2011 | 21683734 |
| molecular characterisation and expression analysis of a fish-egg lectin in rock bream, and its response to bacterial or viral infection. | a fish-egg lectin, rbfel, was identified from rock bream (oplegnathus fasciatus) and its expression analysed. in both vertebrates and invertebrates, carbohydrate-binding proteins (lectins) play an important role in innate immunity against microbial invasion. here, we report the cloning of a fish-egg lectin from rock bream using a combination of expression sequence tag (est) analyses. the full-length cdna of rbfel is composed of 1512-bp with a 780-bp orf that encodes 259 amino acid residues. the ... | 2011 | 22056500 |
| a novel murrel channa striatus mitochondrial manganese superoxide dismutase: gene silencing, sod activity, superoxide anion production and expression. | we have reported the molecular characterization including gene silencing, superoxide activity, superoxide anion production, gene expression and molecular characterization of a mitochondrial manganese superoxide dismutase (mmnsod) from striped murrel channa striatus (named as csmmnsod). the csmmnsod polypeptide contains 225 amino acids with a molecular weight of 25 kda and a theoretical isoelectric point of 8.3. in the n-terminal region, csmmnsod carries a mitochondrial targeting sequence and a s ... | 2014 | 25183231 |
| immunological role of c4 cc chemokine-1 from snakehead murrel channa striatus. | in this study, we have reported a cdna sequence of c4 cc chemokine identified from snakehead murrel (also known as striped murrel) channa striatus (named as cscc-chem-1) normalized cdna library constructed by genome sequencing flx™ technology (gs-flx™). cscc-chem-1 is 641 base pairs (bp) long that contain 438 bp open reading frame (orf). the orf encodes a polypeptide of 146 amino acids with a molecular mass of 15 kda. the polypeptide contains a small cytokine domain at 30-88. the domain carries ... | 2014 | 24231766 |
| fish lily type lectin-1 contains β-prism architecture: immunological characterization. | in this study we report a full-length lily type lectin-1 (csltl-1) identified from striped murrel, channa striatus. csltl-1 was identified from the established c. striatus cdna library using gs-flx™ genome sequencing technology and was found to contain 354 nucleotide base pairs and its open reading frame (orf) encodes a 118 amino acid residue. csltl-1 mrna is predominately expressed in the gills and is up-regulated upon infection with fungus (aphanomyces invadans) and bacteria (aeromonas hydroph ... | 2013 | 23911406 |
| molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells. | toll-like receptor 2 (tlr2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. in this study, the full-length cdna of tlr2 in silvery pomfret (pampus argenteus) was cloned by homology cloning and the rapid amplification of cdna ends (race) technique. the complete cdna sequence of tlr2 was 2932 bp, containing an open reading frame (orf) of 2469 bp encoding 822 amino acids. a multiple alignment analysis of the silvery pomfret tlr2 protein-coding se ... | 2016 | 27574826 |
| characterization of a 1-cysteine peroxiredoxin from big-belly seahorse (hippocampus abdominalis); insights into host antioxidant defense, molecular profiling and its expressional response to septic conditions. | 1-cysteine peroxiredoxin (prx6) is an antioxidant enzyme that protects cells by detoxifying multiple peroxide species. this study aimed to describe molecular features, functional assessments and potential immune responses of prx6 identified from the big-belly seahorse, hippocampus abdominalis (haprx6). the complete orf (666 bp) of haprx6 encodes a polypeptide (24 kda) of 222 amino acids, and harbors a prominent peroxiredoxin super-family domain, a peroxidatic catalytic center, and a peroxidatic ... | 2016 | 27542613 |
| molecular cloning and expression analysis of a new lily-type lectin in the rock bream, oplegnathus fasciatus. | a new lily-type lectin rbltl was identified from rock bream (oplegnathus fasciatus) and its expression analysed. in this study, a new lily-type lectin gene (rbltl) was cloned from rock bream using expressed sequence tag (est) analysis. the full-length rbltl cdna was encoding a 117-amino acid protein. the deduced amino acid sequence of rbltl contained all of the conserved features crucial for its fundamental structure, including b-lectin domain and three d-mannose binding sites. rbltl mrna was pr ... | 2016 | 27345170 |
| thioredoxin domain-containing protein 12 from oplegnathus fasciatus: molecular characterization, expression against immune stimuli, and biological activities related to oxidative stress. | thioredoxin domain-containing protein 12 (txndc12) is a small, disulfide-containing protein that belongs to the thioredoxin (txn) superfamily. in the present study, we identified and characterized a txndc12-like gene, designated oftxndc12, from rock bream, oplegnathus fasciatus. oftxndc12 consists of seven exons interrupted by six introns. comparative genomic structural analysis revealed that the txndc12 of vertebrates is a structurally conserved gene. the coding sequence of oftxndc12 comprises ... | 2016 | 27026037 |
| antimicrobial response of galectin-1 from rock bream oplegnathus fasciatus: molecular, transcriptional, and biological characterization. | in this study, we describe the identification and characterization of a proto type galectin, galectin-1, from rock bream oplegnathus fasciatus (ofgal-1). galectins are evolutionarily conserved carbohydrate binding lectins that show a wide range of functions related to development and immune physiology. they have been identified as pattern recognition receptors of innate immune system that recognize a broad range of microbes. ofgal-1 cdna comprised of 993 bp with an open reading frame of 408 bp t ... | 2016 | 26792759 |
| coagulation factor ii from rock bream (oplegnathus fasciatus): first report on the molecular biological function and expression analysis in the teleost. | the rapid haemostasis of fish prevents bleeding or infection that could be caused by physical properties of the aquatic environment. additionally, the innate immune system is the first line of defence against infection and is responsible for the recognition of pathogen-associated molecular patterns, which are important for the activation of acquired immune responses. coagulation factor ii (cfii) is an important factor in the coagulation system and is involved in recognition and interaction with ... | 2016 | 26626585 |
| a galectin related protein from oplegnathus fasciatus: genomic, molecular, transcriptional features and biological responses against microbial pathogens. | galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. here, we report a novel galectin related protein (grp) identified from rock bream (oplegnathus fasciatus), designated ofgal like b. the cdna of ofgal like b is 517 bp with an open reading frame (orf) of 438 bp, encoding 145 amino acids, with a s ... | 2016 | 26615008 |
| molecular, genomic, and expressional delineation of a piscidin from rock bream (oplegnathus fasciatus) with evidence for the potent antimicrobial activities of of-pis1 peptide. | the piscidin family comprises a group of antimicrobial peptides (amps) that are vital components of teleost innate immunity. piscidins protect the host from pathogens, through multifaceted roles as immunomodulators and anti-infective peptides. the present study reports the identification, and characterization of a putative piscidin homolog, of-pis1, from rock bream (oplegnathus fasciatus). a combined genomic and transcriptomic approach revealed that the of-pis1 gene comprises 1396 nucleotides (n ... | 2016 | 26549174 |
| first description of programmed cell death10 (pdcd10) in rock bream (oplegnathus fasciatus): potential relations to the regulation of apoptosis by several pathogens. | in this study, we isolated and characterized programmed cell death10 (pdcd10), which is known to be related to apoptosis, from rock bream (oplegnathus fasciatus). the full-length rock bream pdcd10 (rbpdcd10) cdna (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among the other pdcd10 sequences tested. rbpdcd10 was significantly expressed in the liver, rbc (red blood c ... | 2016 | 26472617 |
| gene expression and functional characterization of serum amyloid p component 2 in rock bream, oplegnathus fasciatus. | mammalian serum amyloid p component (sap) recognizes a wide range of exogenous pathogenic substances and activates a complementary pathway leading to pathogen clearance. to determine the potential roles of sap in the fish immune system, sap (rbsap2) gene was cloned from ests analysis of rock bream (oplegnathus fasciatus), which consisted of a signal peptide and pentraxin domain. phylogenetic analysis revealed that the rbsap2 gene was classified with other known fish saps. rbsap2 was highly expre ... | 2015 | 26455663 |
| gene structure, molecular characterization and transcriptional expression of two p38 isoforms (mapk11 and mapk14) from rock bream (oplegnathus fasciatus). | the p38 kinases are one of the four subgroups of mitogen-activated protein kinase (mapk) superfamily which are involved in the innate immunity. the p38 subfamily that includes four members namely p38α (mapk14), p38β (mapk11), p38γ (mapk12) and p38δ (mapk13), regulates the activation of several transcription factors. in this study, a p38β (ofmapk11) homolog and a p38α (ofmapk14) homolog of oplegnathus fasciatus were identified at genomic level. results clearly showed that both mapk11 and mapk14 a ... | 2015 | 26363230 |
| characterization of rock bream (oplegnathus fasciatus) complement components c1r and c1s in terms of molecular aspects, genomic modulation, and immune responsive transcriptional profiles following bacterial and viral pathogen exposure. | the complement components c1r and c1s play a crucial role in innate immunity via activation of the classical complement cascade system. as initiators of the pathogen-induced signaling cascade, c1r and c1s modulate innate immunity. in order to understand the immune responses of teleost c1r and c1s, oplegnathus fasciatus c1r and c1s genes (ofc1r and ofc1s) were identified and characterized. the genomic sequence of ofc1r was enclosed with thirteen exons that represented a putative peptide with 704 ... | 2015 | 26241508 |
| a homolog of kunitz-type serine protease inhibitor from rock bream, oplegnathus fasciatus: molecular insights and transcriptional modulation in response to microbial and pamp stimulation, and tissue injury. | serine proteases and their inhibitors play vital roles in diverse biological processes. in this study, we identified and characterized cdna coding for a kunitz-type serine protease inhibitor (spi), which we designated as rbkspi, in a commercially important species, rock bream. the full-length cdna sequence of rbkspi consisted of 2452 bp with an open reading frame (orf) of 1521 bp encoding a polypeptide of 507 amino acid (aa) residues. in the rbkspi protein, manec, pkd, ldla, and two kunitz domai ... | 2015 | 26162478 |
| first molecular characterisation and expression analysis of a teleost thioredoxin-interacting protein (txnip) gene from rock bream (oplegnathus fasciatus). | thioredoxin-interacting protein (txnip) is an important regulator of glucose metabolism that functions by inhibiting cellular glucose uptake. the full-length rock bream (oplegnathus fasciatus) txnip (rbtxnip) cdna (2499 bp) contains an open reading frame of 1188 bp encoding 396 amino acids. furthermore, multiple alignments showed that the arrestin domain was well conserved among the other txnip sequences tested. rbtxnip was predicted to contain a pxxp and ppxy motif. phylogenetic analysis indica ... | 2015 | 25934185 |
| a potential antioxidant enzyme belonging to the atypical 2-cys peroxiredoxin subfamily characterized from rock bream, oplegnathus fasciatus. | peroxiredoxins (prxs), a diverse family of antioxidant enzymes, exert their antioxidant function through which different peroxide species are detoxified. this study describes both structural and functional characterization of a mitochondrial prx identified in rock bream, oplegnathus fasciatus (rbprx5). the orf (573 bp) of rbprx5 encoded a protein of 190 amino acids (20 kda) containing a putative mitochondrial targeting sequence (residues 1-20) and a thioredoxin-2 motif (residues 31-190) and thre ... | 2015 | 25934084 |
| functional characterisation and expression analysis of recombinant serum amyloid p isoform 1 (rbsap1) from rock bream (oplegnathus fasciatus). | lectins are carbohydrate-binding proteins that play important roles in the recognition and elimination of pathogens via the innate immune system. pentraxins (ptx) are humoral lectins, which are multifunctional proteins in vertebrates. pentraxins can be divided into two groups based on their primary structure: short (c-reactive protein and serum amyloid p [sap]) and long pentraxins (ptx3 and neuronal pentraxins). previously, sap was shown to have ca(2+)-dependent binding specificity for various l ... | 2015 | 25917975 |
| insights into molecular profiles and genomic evolution of an irak4 homolog from rock bream (oplegnathus fasciatus): immunogen- and pathogen-induced transcriptional expression. | as a pivotal signaling mediator of toll-like receptor (tlr) and interleukin (il)-1 receptor (il-1r) signaling cascades, the il-1r-associated kinase 4 (irak4) is engaged in the activation of host immunity. this study investigates the molecular and expressional profiles of an irak4-like homolog from oplegnathus fasciatus (ofirak4). the ofirak4 gene (8.2 kb) was structured with eleven exons and ten introns. a putative coding sequence (1395bp) was translated to the ofirak protein of 464 amino acids. ... | 2015 | 25555811 |
| mitochondrial peroxiredoxin 3 (prx3) from rock bream (oplegnathus fasciatus): immune responses and role of recombinant prx3 in protecting cells from hydrogen peroxide induced oxidative stress. | pathogenic infections and environmental factors cause a variety of stresses in fish including oxidative stress by rapid elevation of reactive oxygen species (ros) and reactive nitrogen species (rns). transcriptional activation and expression of antioxidant enzymes are essential for reducing the oxidative stress. in this study, we present the molecular characterization, immune responses and ros scavenging activity of mitochondrial peroxiredoxin 3 from oplegnathus fasciatus (rbprx3). coding sequen ... | 2015 | 25542382 |
| two nf-κb inhibitor-alpha (iκbα) genes from rock bream (oplegnathus fasciatus): molecular characterization, genomic organization and mrna expression analysis after immune stimulation. | ikba is a member of ikb family, which sequesters nf-kb in an inactivate form in the cytoplasm and blocks the translocation of nf-kb to nucleus. the ikba paralogs of rock bream (ofikba-a and ofikba-b) encoded ikba proteins with typical features including, highly conserved ikb degradation motif, six ankyrin repeats and a pest sequence. however, their amino acid identity and similarity were only 55.6 and 69.7%, respectively suggesting that these two genes could be the two different isoforms of ikba ... | 2014 | 25462458 |
| two carboxypeptidase counterparts from rock bream (oplegnathus fasciatus): molecular characterization, genomic arrangement and immune responses upon pathogenic stresses. | carboxypeptidases (cps) are proteases that hydrolyze c-terminal peptide bonds. they are involved in regulating the complement system of the immune system. here, we report the molecular characterization and immune response of two carboxypeptidases, named carboxypeptidase a (rb-cpa) and carboxypeptidase n1 (rb-cpn1), from rock bream. the genomic sequence of rb-cpa contains 12 exons interrupted by 11 introns, while the genomic sequence of rb-cpn1 has 9 exons and 8 introns. the cdna sequence of rb-c ... | 2014 | 25457548 |
| molecular genomic- and transcriptional-aspects of a teleost traf6 homolog: possible involvement in immune responses of oplegnathus fasciatus against pathogens. | tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6) is a crucial docking molecule for tnfr superfamily and interleukin-1 receptor/toll-like receptor (il-1r/tlr) superfamily. as an adaptor protein in pathogen-induced signaling cascades, traf6 modulates both adaptive- and innate-immunity. in order to understand the immune responses of teleost traf6, oplegnathus fasciatus traf6-like gene (oftraf6) was identified and characterized. genomic length of oftraf6 (4 kb), obtained by means of ... | 2015 | 25449707 |
| first molecular cloning and gene expression analysis of teleost cd42 (glycoprotein ib beta chain) gpib-ix-v subunit from rock bream, oplegnathus fasciatus. | cd42 is a platelet membrane glycoprotein ib that plays a key role in haemostasis and thrombin-induced platelet activation. here, we report the molecular cloning and sequence analysis of the cd42c gene from rock bream (oplegnathus fasciatus). rock bream cd42 (rbcd42c) gene expression profiles were determined after infection with streptococcus iniae, edwardsiella tarda and red seabream iridovirus (rsiv). the full-length rbcd42c cdna contained an open reading frame of 624 bp encoding 207 amino acid ... | 2015 | 25445900 |
| molecular characterization and transcriptional analysis of non-mammalian type toll like receptor (tlr21) from rock bream (oplegnathus fasciatus). | toll-like receptors (tlrs) are a large family of pattern recognition receptors, which are involved in triggering host immune responses against various pathogens by detecting their evolutionarily conserved pathogen associated molecular patterns (pamps). tlr21 is a non-mammalian type tlr, which recognizes unmethylated cpg dna, and is considered as a functional homolog of mammalian tlr9. in this study, we attempted to identify and characterize a novel tlr21 counterpart from rock bream (oplegnathus ... | 2014 | 25300254 |
| expression analysis and biological activity of moronecidin from rock bream, oplegnathus fasciatus. | the piscidin-family, one of antimicrobial peptides (amps) mainly distributed in fish, is crucial effectors of fish innate immune response. piscidin-family typically has broad-spectrum antimicrobial activity and the ability to modulate the immune response. in this study, we identified moronecidin (rbmoro) included in piscidin-family from rock bream and investigated its gene expression using quantitative real-time pcr and biological activity (including antimicrobial and cytotoxic activity). the co ... | 2014 | 25050859 |
| cloning, characterisation, and expression analysis of the cathepsin d gene from rock bream (oplegnathus fasciatus). | cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for the maintenance of cellular homeostasis. rock bream (oplegnathus fasciatus) cathepsin d (rbctsd) cdnas were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cdna library. the full-length rbctsd cdna (1644 bp) contained an open reading frame of 1191 bp encoding 396 amino acids. alignment analysis revealed that t ... | 2014 | 25038285 |
| molecular aspects, genomic arrangement and immune responsive mrna expression profiles of two cxc chemokine receptor homologs (cxcr1 and cxcr2) from rock bream, oplegnathus fasciatus. | the cxcr1 and cxcr2 are the prototypical receptors and are the only known receptors for mammalian elr+ (glu-leu-arg) cxc chemokines, including cxcl8 (interleukin 8). these receptors transduce the elr+ chemokine signals and operate the downstream signaling pathways in inflammation and innate immunity. in this study, we report the identification and characterization of cxcr1 and cxcr2 genes from rock bream fish (ofcxcr1 and ofcxcr2) at the molecular level. the cdna and genomic dna sequences of the ... | 2014 | 24945570 |
| molecular cloning and expression analysis of two lipopolysaccharide-induced tnf-α factors (litafs) from rock bream, oplegnathus fasciatus. | lipopolysaccharide (lps)-induced tumor necrosis factor (tnf)-α factor (litaf) plays an important role controlling the expression of tnf-α and the other cytokine genes in the presence of lps. however, two litaf homologues have not been characterized in fish. in this study, we cloned two distinct litaf (rblitaf1 and rblitaf2) cdnas from rock bream (oplegnathus fasciatus) and characterized their expression profiles after infection with edwardsiella tarda, streptococcus iniae or red seabream iridovi ... | 2014 | 24394623 |
| genomic structure and immunological response of an stat4 family member from rock bream (oplegnathus fasciatus). | the janus tyrosine kinase (jak)/signal transducer and activator of transcription (stat) signaling pathway plays a critical role in host defense against viral and bacterial infections. stat proteins are a group of transcription factors that translocate into the nucleus and are critical for the induction of many genes crucial for the allergic cascade and immune defense. in the present study, a member of the stat4 family was identified from rock bream (rbstat4) at the genomic level, and its transcr ... | 2013 | 24055510 |
| three complement component 1q genes from rock bream, oplegnathus fasciatus: genome characterization and potential role in immune response against bacterial and viral infections. | complement component 1q (c1q) is a subcomponent of the c1 complex and the key protein that recognizes and binds to a broad range of immune and non-immune ligands to initiate the classical complement pathway. in the present study, we identified and characterized three novel c1q family members from rock bream, oplegnathus fasciatus. the full-length cdnas of c1q a-like (rbc1qal), c1q b-like (rbc1qbl), and c1q c-like (rbc1qcl) consist of 780, 720 and 726 bp of nucleotide sequence encoding polypeptid ... | 2013 | 23994081 |
| a teleostean counterpart of ferritin m subunit from rock bream (oplegnathus fasciatus): an active constituent in iron chelation and dna protection against oxidative damage, with a modulated expression upon pathogen stress. | ferritins are biological iron chelators that can sequestrate excess iron to maintain iron homeostasis in the body. ferritins basically consist of 2 types of subunits, designated as h and l. however, another new subunit, ferritin "m" which possesses characteristic features of both the h and l subunits, was recently identified in lower vertebrates, mostly in fish. in this study, a ferritin m-like subunit from rock bream (oplegnathus fasciatus) (rbferm) was characterized at the molecular level, and ... | 2013 | 23978565 |
| genomic characterization, expression analysis, and antimicrobial function of a glyrichin homologue from rock bream, oplegnathus fasciatus. | antimicrobial peptides are important innate effector molecules, playing a vital role in antimicrobial immunity in all species. glyrichin is a transmembrane protein and an antibacterial peptide, exerting its functions against a wide range of pathogenic bacteria. in this study, cdna and a bac clone harboring the glyrichin gene were identified from rock bream and characterized. genomic characterization showed that the ofglyrichin gene exhibited a 3 exon-2 intron structure. ofglyrichin is a 79-amino ... | 2013 | 23968692 |
| molecular cloning and expression analysis of interleukin (il)-15 and il-15 receptor α from rock bream, oplegnathus fasciatus. | mammalian interleukin (il)-15 plays an important role in the activation of cd8(+) t cells and natural killer (nk) cells along with its receptor α (il-15rα). to understand the potential roles of il-15 and il-15rα in fish, we identified il-15 and il-15rα cdna from rock bream (oplegnathus fasciatus) and investigated their gene expression profiles after bacterial and viral infection. coding regions of rock bream (rb) il-15 and rbil-15rα cdnas were 534 and 402 bp encoding 177 and 133 amino acid resid ... | 2013 | 23911652 |
| marine teleost ortholog of catalase from rock bream (oplegnathus fasciatus): molecular perspectives from genomic organization to enzymatic behavior with respect to its potent antioxidant properties. | catalases are well known antioxidant enzymes that can mainly dismutate hydrogen peroxide into water and oxygen in order to prevent oxidative stress. the complete genomic dna (gdna) sequence of the catalase gene from rock bream (oplegnathus fasciatus) was identified from our custom-constructed bac genomic dna library and designated as rbcat. rbcat consists of 13 exons, separated by 12 introns, within a 13,722-bp gdna sequence. the complete cdna sequence (3303 bp) of rbcat is comprised of a 1581-b ... | 2013 | 23872475 |
| akirin2 homologues from rock bream, oplegnathus fasciatus: genomic and molecular characterization and transcriptional expression analysis. | akirins are conserved nuclear resident nf-κb signaling pathway molecules. isoforms of akirins found in various organisms are known to play diverse roles. in this study, we have characterized two akirin2 homologues from rock bream, ofak2(1) and ofak2(2). the proteins derived from ofak2(1) and ofak2(2) revealed the presence of nuclear localization signal. multiple sequence alignment and pairwise alignment of ofak2(1) and ofak2(2) with the akirin homologues, revealed high conservation and identity. ... | 2013 | 23770624 |
| molecular characterisation and expression analysis of the cathepsin h gene from rock bream (oplegnathus fasciatus). | cathepsins are lysosomal cysteine proteases belonging to the papain family, whose members play important roles in normal metabolism for the maintenance of cellular homeostasis. rock bream (oplegnathus fasciatus) cathepsin h (rbctsh) cdnas were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cdna library. the full-length rbctsh cdna (1326 bp) contained an open reading frame of 978 bp encoding 325 amino acids. the presence of an erfnin-like motif w ... | 2013 | 23629026 |
| genomic characterization and transcriptional evidence for the involvement of complement component 7 in immune response of rock bream (oplegnathus fasciatus). | the complement component 7 (c7) is the central mediator of pathogenic attack at the membrane surface and its binding to the c5b-7 complex triggers cytolytic signaling. in this study, c7 of rock bream (oplegnathus fasciatus) was identified (rb-c7) and characterized at the genomic level. the rb-c7 gene contains 18 exons and 17 introns and is composed of a 2490 bp complete open reading frame (orf). the encoded polypeptide (830 amino acids) contains a number of well-conserved c7 signature domains. i ... | 2013 | 23603298 |
| molecular characterisation and biological activity of a novel cxc chemokine gene in rock bream (oplegnathus fasciatus). | chemokines are chemoattractant cytokines defined by the presence of four conserved cysteine residues. in mammals, these cytokines can be divided into four subfamilies depending on the arrangement of the first two conserved cysteines in the sequence, and include the cxc(α), cc(β), c(γ), and cx3c(δ) classes. we identified cxc chemokine cdna, designated rbcxc, isolated using expressed sequence tag analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the full-length rbcxc ... | 2013 | 23376472 |
| first molecular cloning and gene expression analysis of a teleost cd200 (ox-2) glycoprotein from rock bream, oplegnathus fasciatus. | cd200 plays an important role in delivering an immunoregulatory signal to the immune system through interaction with its receptor. however, cd200 has not been characterized and its function in teleosts is unknown. in this study, the rock bream (oplegnathus fasciatus) cd200 gene (rbcd200) was cloned and its expression profile was analyzed after infection with edwardsiella tarda, streptococcus iniae or red seabream iridovirus (rsiv). the coding region of rbcd200 cdna was 855 bp, encoding 284 amino ... | 2013 | 23128045 |
| genomic characterization and expression analysis of complement component 8α and 8β in rock bream (oplegnathus fasciatus). | the complement component 8α and 8β are glycoproteins that mediate formation of the membrane attack complex (mac) on the surface of target cells. full-length complement c8α (rb-c8α) and c8β (rb-c8β) sequences were identified from a cdna library of rock bream (oplegnathus fasciatus), and their genomic sequences were obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library of rock bream. the rb-c8α gene contains 64bp of 5'-utr, open reading frame (orf) of ... | 2013 | 23059376 |
| a manganese superoxide dismutase with potent antioxidant activity identified from oplegnathus fasciatus: genomic structure and transcriptional characterization. | in this study, we describe the identification and characterization of manganese superoxide dismutase, an important antioxidant enzyme acting as the chief reactive oxygen species (ros) scavenger, from rock bream oplegnathus fasciatus (of-mmnsod) at genomic- and transcriptional-levels as well as the biological activity of recombinant protein. the of-mmnsod protein portrayed distinct mnsod family features including signature motifs, metal association sites and the typical active site topology. it w ... | 2013 | 23022055 |
| molecular cloning and functional characterization of two duplicated two-cysteine containing type i interferon genes in rock bream oplegnathus fasciatus. | two type i interferon (ifn) genes, designated as rbifn1 and rbifn2, have been cloned and characterized in rock bream. they are both comprised of 5 exons and 4 introns, and are closely linked on the rock bream chromosome in a unique head-to-head configuration. both genes encode 183 amino acid (aa) precursor with a putative 17 aa signal peptide in the n-terminal. only one amino acid divergence is present between two ifns. compared with the type i ifns in higher vertebrates, two rock bream ifns pos ... | 2012 | 22889848 |
| interferon regulatory factors 4 and 8 in rock bream, oplegnathus fasciatus: structural and expressional evidence for their antimicrobial role in teleosts. | the interferon regulatory factor (irf) members irf4 and irf8 contribute to b-lymphocyte development and can act as regulators of immunoglobulin (ig) light chain gene transcription. these two irfs are closely interrelated and are expressed at high levels in the lymphoid and myeloid cells of the immune system. in this study, the complete cdna and genomic sequences of rock bream irf4 (rbirf4) and irf8 (rbirf8) were identified by homology screening of a multi-tissue normalized cdna library and a bac ... | 2012 | 22885028 |
| genomic characterization and expression analysis of complement component 9 in rock bream (oplegnathus fasciatus). | the complement component 9 (c9) is a single-chain glycoprotein that mediates formation of the membrane attack complex (mac) on the surface of target cells. full-length c9 sequence was identified from a cdna library of rock bream (oplegnathus fasciatus), and its genomic sequence was obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library of rock bream. the rock bream complement component 9 (rb-c9) gene contains 11 exons and 10 introns and is composed of ... | 2012 | 22796422 |
| cystatin b homolog from rock bream oplegnathus fasciatus: genomic characterization, transcriptional profiling and protease-inhibitory activity of recombinant protein. | cysteine proteases are present in all living organisms and, in animals, function in a vast array of physiological and pathological processes. cysteine protease inhibitors act upon the cysteine proteases to regulate their activity. the cystatin superfamily of cysteine protease inhibitors has members represented in all living organisms studied to date. here, we report the identification of a new member of the family 1 cystatin in oplegnathus fasciatus rock bream (denoted as rbcyt b) and the charac ... | 2012 | 22626887 |
| caspase 3 from rock bream (oplegnathus fasciatus): genomic characterization and transcriptional profiling upon bacterial and viral inductions. | caspase 3 is a prominent mediator of apoptosis and participates in the cell death signaling cascade. in this study, caspase 3 was identified (rbcasp3) and characterized from rock bream (oplegnathus fasciatus). the full-length cdna of rbcasp3 is 2683 bp and contains an open reading frame of 849 bp, which encodes a 283 amino acid protein with a calculated molecular mass of 31.2 kda and isoelectric point of 6.31. the amino acid sequence resembles the conventional caspase 3 domain architecture, incl ... | 2012 | 22554851 |
| molecular identification and expression analysis of two distinct bpi/lbps (bactericidal permeability-increasing protein/lps-binding protein) from rock bream, oplegnathus fasciatus. | we identified two cdnas designated as rbbpi/lbp-1 and rbbpi/lbp2, respectively, which were identified by expressed sequence tag (est) analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the two cdna displayed 36.9% identity at the translated amino acid level. despite the low level of identity between the two genes, high conservation was seen in the bpi/lbp/cetp n-terminal, lps-binding, the proline-rich central and the bpi/lbp/cetp c-terminal domains. the full-length ... | 2012 | 22521422 |
| a novel acute phase reactant, serum amyloid a-like 1, from oplegnathus fasciatus: genomic and molecular characterization and transcriptional expression analysis. | acute phase response is a significant component of innate immunity, playing a vital role in the signaling processes and elimination of invading pathogens. acute phase proteins are synthesized in liver and secreted into the blood for transportation to an infection site, where the defense function is exerted. serum amyloid a (saa) and c-reactive proteins are the major positive acute phase proteins. in this study, we have identified and characterized a novel saa related gene from rock bream (oplegn ... | 2012 | 22504166 |
| a teleostean angiotensinogen from oplegnathus fasciatus responses to immune and injury challenges. | angiotensinogen (agt) is the precursor of the renin-angiotensin system and contributes to osmoregulation, acute-phase and immune responses. a full-length cdna of the agt (2004 bp with a 1389 bp coding region) was isolated from rock bream (rb), oplegnathus fasciatus. the encoded polypeptide of 463 amino acids had a predicted molecular mass of 51.6 kda. rbagt possessed a deduced signal peptide of 22 residues upstream of a putative angiotensin i sequence ((23)nrvyvhpfhl(32)). rbagt possessed a spec ... | 2012 | 22300787 |
| molecular identification and expression analysis of a natural killer cell enhancing factor (nkef) from rock bream oplegnathus fasciatus and the biological activity of its recombinant protein. | natural killer cell enhancing factor (nkef) belongs to the defined peroxiredoxin (prx) family. rock bream nkef cdna was identified by expressed sequence tag (est) analysis of rock bream liver that was stimulated with the lps. the full-length rbnkef cdna (1062 bp) contained an open reading frame (orf) of 594 bp encoding 198 amino acids. rbnkef was significantly expressed in the gill, liver, and intestine. mrna expression of nkef in the head kidney was examined under viral and bacterial challenge ... | 2011 | 24371552 |
| a c-type lectin that inhibits bacterial infection and facilitates viral invasion in black rockfish, sebastes schlegelii. | c-type lectins (ctls) are important pattern recognition receptors (prrs) that play vital roles in innate immunity. in teleosts, a number of ctls have been reported, but their in vivo effects on host defense are still limited. in this study, a ctl homolog (sslec1) was identified from black rockfish, sebastes schlegelii, and its structure, expression and biological function was analyzed. the open reading frame of sslec1 is 633 bp, with a 5'- untranslated region (utr) of 36 bp and a 3'- utr of 117 ... | 2016 | 27569982 |
| thioredoxin of golden pompano involved in the immune response to photobacterium damselae. | thioredoxin (trx) is one of the key systems responsible for keeping the intracellular environment in a highly reduced state. in this study, a full-length trx cdna sequence (totrx) from golden pompano trachinotus ovatus was identified after pyrosequencing of golden pompano cdna library. totrx cdna is comprised of 786 bp, and contained a 324 bp open reading frame (orf) encoding a 107 amino acid polypeptide, a 5' untranslated region (utr) of 116 bp, and a long 3'- utr of 346 bp. multiple sequence a ... | 2015 | 26052015 |
| characterization of mif family proteins: mif and ddt from rock bream, oplegnathus fasciatus. | macrophage migration inhibitory factor (mif) is a pleiotropic molecule playing vital roles in various signaling cascades, including cell proliferation, and activation of immune responses against infections. it is well known as a pivotal regulator of innate immunity. in this study, we have rescued and characterized two members of the mif family, macrophage migration inhibitory factor (ofmif) and d-dopachrome tautomerase (ofddt) from rock bream, oplegnathus fasciatus. the deduced ofmif and ofddt p ... | 2013 | 23688964 |
| molecular identification and expression analysis of the cc chemokine gene in rock bream (oplegnathus fasciatus) and the biological activity of the recombinant protein. | we identified the cc chemokine cdna designated as rbcc1 (cc chemokine 1 in rock bream, oplegnathus fasciatus), which was isolated using expressed sequence tag (est) analysis of a lipopolysaccharide (lps)-stimulated rock bream liver cdna library. the full-length rbcc1 cdna (850 bp) contained an open reading frame (orf) of 366 bp encoding 122 amino acids. results from our phylogenetic analysis demonstrated that the rbcc1 was closest relationship to the orange-spotted grouper and mi-iyu croaker cc ... | 2013 | 23357024 |
| cloning, characterization and expression analysis of coagulation factor ii gene in grass carp (ctenopharyngodon idella). | here, we characterized the structure and function of the coagulation factor ii (fii) gene in grass carp and determined its role in coagulation mechanisms. the fii gene est was obtained using a constructed splenic transcriptome database; the full-length fii gene sequence was obtained by 3' and 5' race. the open reading frame (orf) of fii was cloned and the full-length gene was found to be 1718 bp, with an orf of 1572 bp; the gene contained a 25 bp 5'-untranslated region (utr) and 108 bp 3'-utr. t ... | 2015 | 26535692 |
| amoebic gill disease outbreak in marine fish cultured in korea. | in 2015, 6.7-60% mortality was observed in black seabream ( acanthopagrus schlegelii), rock bream ( oplegnathus fasciatus), and gray mullet ( mugil cephalus) farmed in the southern coast of korea. on examination, numerous amoebae were found on the gills of these 3 fish species with detection rate of 100%. some rock bream and gray mullet were coinfected with bacteria ( pseudomonas anguilliseptica, vibrio tapetis, or vibrio anguillarum). histologic examination revealed extensive hyperplastic epith ... | 2017 | 28205457 |
| establishment of rock bream oplegnathus fasciatus embryo (robe-4) cells with cytolytic infection of red seabream iridovirus (rsiv). | red seabream iridovirus (rsiv) is a member of genus megalocytivirus in the family iridoviridae. rsiv infection causes significant economic losses of marine-fishes in east asian countries. grunt fin (gf) cell line has been commonly used for culturing rsiv. however, it is not suitable for definite evaluation of infectivity titer of rsiv because cells infected with rsiv are not completely cytolysed. thus, we established a new cell line, robe-4, from rock bream (oplegnathus fasciatus) eyed-egg embry ... | 2016 | 27688178 |
| potential for a live red seabream iridovirus (rsiv) vaccine in rock bream oplegnathus fasciatus at a low rearing temperature. | serious economic losses have occurred in fingerlings and market-sized rock bream oplegnathus fasciatus in korea due to red seabream iridovirus (rsiv) infection. we demonstrated previously that viral multiplication in fish is downregulated by maintaining fish at far from optimum temperatures at the onset of disease. we applied this concept to develop a live rsiv vaccine in rock bream. mortalities in rock bream that were inoculated with rsiv and reared at 21-30°c were ≥90%, whereas no mortality wa ... | 2014 | 24252702 |
| antiviral function of grouper mda5 against iridovirus and nodavirus. | melanoma differentiation-associated gene 5 (mda5) is a critical member of retinoic acid-inducible gene i (rig-i)-like receptor (rlr) family which can recognize viral rna and enhances antiviral response in host cells. in this study, a mda5 homolog from orange spotted grouper (epinephelus coioides) (ecmda5) was cloned, and its roles on grouper virus infection were characterized. the full-length ecmda5 cdna encoded a polypeptide of 982 amino acids with 74% identity with mda5 homolog from rock bream ... | 2016 | 27050314 |
| expression analysis of lily type lectin isotypes in the rock bream, oplegnathus fasciatus: in the tissue, developmental stage and viral infection. | lectins belong to the pattern-recognition receptors (prrs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. however, this study is limited to information that the lectin is involved in the innate immune response against viral infection. in the present study, the lily-type lectin-2 and -3 of oplegnathus fasciatus (o ... | 2016 | 28144635 |
| complement factor d homolog involved in the alternative complement pathway of rock bream (oplegnathus fasciatus): molecular and functional characterization and immune responsive mrna expression analysis. | the complement system serves conventional role in the innate defense against common invading pathogens. complement factor d (cfd) is vital to alternative complement pathway activation in cleaving complement factor b. this catalytic reaction forms the alternative c3 convertase that is crucial for complement-mediated pathogenesis. in this study, rock bream (oplegnathus fasciatus) cfd (ofcfd) was characterized and ofcfd mrna expression was investigated. ofcfd encodes 277 amino acids (aa) for a 30-k ... | 2016 | 27311435 |
| effects of water temperature on mortality in megalocytivirus-infected rock bream oplegnathus fasciatus (temminck et schlegel) and development of protective immunity. | rock bream iridovirus (rbiv) causes huge losses, especially in rock bream oplegnathus fasciatus. rock bream injected with rbiv and held at 29, 26, 23 or 20 °c had 100% mortality. conversely, all infected fish held at 17 °c survived even after the temperature was progressively increased to 26 °c at 100 dpi. rock bream exposed to virus and held for 2, 4 and 7 days at 23/26 °c before the temperature was reduced to 17 °c had mortality rates of 26.6/73.2%, 66.6/100% and 93.4/100%, respectively, throu ... | 2015 | 25073547 |
| gene expression of pro- and anti-apoptotic proteins in rock bream (oplegnathus fasciatus) infected with megalocytivirus (family iridoviridae). | viruses belonging to the genus megalocytivirus cause diseases in marine fishes primarily in east and southeast asian countries. rock bream iridovirus (rbiv), which is a member of the megalocytivirus genus, causes severe mass mortalities in rock beam (oplegnathus fasciatus) in korea. in this study, we assessed apoptosis-related gene expression patterns in megalocytivirus-infected rock bream in high mortality and low mortality conditions to determine important apoptosis-related factors, which may ... | 2014 | 24463468 |
| recombinant thioredoxin 1 protein: the immune-adjuvant effect of streptococcus iniae and its safety in rock bream, oplegnathus fasciatus. | adjuvant is an immune enhancer commonly used during vaccination to enhance the host immune response. in the present study, we analysed the recombinant protein produced from rock bream thioredoxin 1 cdna (rrbtrx1). to verify the immune-stimulatory effect of this recombinant protein, changes in the expression level of several genes were investigated using the cdna microarray chips in rock bream peripheral blood leukocytes stimulated with rrbtrx1. furthermore, the immune responses of rock bream to ... | 2014 | 24830770 |
| genomic identification and molecular characterization of a non-mammalian tnfaip8l2 gene from oplegnathus fasciatus. | tumor necrosis factor alpha-induced protein 8-like 2 (tnfaip8l2) is a newly described negative immune regulator, whose enigmatic biological functions are not clearly understood. in the present study, the tnfaip8l2 homolog of rock bream (oplegnathus fasciatus) was identified and characterized. the genomic composition of rock bream tnfaip8l2 (~6.7 kb) represented a tripartite arrangement in which three exons are interrupted by two introns. the rock bream tnfaip8l2 transcript (1974 bp) possessed a ... | 2014 | 24583177 |
| a c1 inhibitor ortholog from rock bream (oplegnathus fasciatus): molecular perspectives of a central regulator in terms of its genomic arrangement, transcriptional profiles and anti-protease activities of recombinant peptide. | c1 inhibitor (c1inh), a member of serpin superfamily, is a crucial regulator of the activation of various plasmatic cascades associated with immunity and inflammation. this study describes the identification and characterization of a c1inh gene from rock bream oplegnathus fasciatus (ofc1inh) at structural, expressional and functional levels. the cdna-(2245bp) and corresponding gdna-sequences (5.2kbp) of ofc1inh were isolated from rock bream transcriptome- and bac-libraries, respectively. predict ... | 2014 | 24056051 |
| molecular cloning and characterisation of the rock bream, oplegnathus fasciatus, fas (cd95/apo-1), and its expression analysis in response to bacterial or viral infection. | fas belongs to the tumour necrosis factor (tnf) receptor superfamily and can transmit a death signal leading to apoptosis. in the present study, we isolated the full-length cdna for rock bream (oplegnathus fasciatus) fas (rbfas). the full-length rbfas cdna was 1770 bp long and contained an open reading frame of 957 bp that encoded 319 amino acid residues with a predicted molecular mass of 35.1 kda. the 319 amino-acid predicted rbfas sequence is homologous to other fas sequences, contains three c ... | 2011 | 24371547 |
| quantitative real time rt-pcr study of pathogen-induced gene expression in rock bream (oplegnathus fasciatus): internal controls for data normalization. | rock bream oplegnathus fasciatus is an important economic fish species. in this study, we evaluated the appropriateness of six housekeeping genes as internal controls for quantitative real-time pcr (rt-qpcr) analysis of gene expression in rock bream before and after pathogen infection. the expression of the selected genes in eight tissues infected with vibrio alginolyticus or megalocytivirus was determined by rt-qpcr, and the pcr data were analyzed with genorm and normfinder algorithms. the resu ... | 2014 | 24657097 |
| comparative genomic of the teleost cathepsin b and h and involvement in bacterial induced immunity of miiuy croaker. | cathepsins are a family of lysosomal proteases play different roles at physiological and pathological states and present in almost all animals as well as other organisms. cathepsins b and h are both cysteine proteases of cathepsins. cathepsin b and h have been studied playing parts in protein degradation/turnover, antigen presentation/processing and hormone maturation in mammals. however, little is known about the structures and functions of cathepsin b and h in teleosts. in the present study, w ... | 2014 | 25181651 |
| modulation of proteome expression by f-type lectin during viral hemorrhagic septicemia virus infection in fathead minnow cells. | lectins found in fish tissues play an important role in the innate immune response against viral infection. a fucose-binding type lectin, rbftl-3, from rock bream (oplegnathus fasciatus) was identified using expressed sequence tag (est) analysis. the expression of rbftl-3 mrna was higher in intestine than other tissues of rock bream. to determine the function of rbftl-3, vhsv-susceptible fathead minnow (fhm) cells were transfected with pcdna3.1(+) or pcdna3.1(+)-rbftl-3 and further infected with ... | 2014 | 24931624 |
| a proto-type galectin-2 from rock bream (oplegnathus fasciatus): molecular, genomic, and expression analysis, and recognition of microbial pathogens by recombinant protein. | a β-galactoside binding lectin, designated as galectin-2, was identified and characterized from rock bream oplegnathus fasciatus (ofgal-2). the cdna of ofgal-2 comprised of 692 bp with a coding sequence of 396 bp, encoding a putative polypeptide of 131 amino acids. gene structure analysis of ofgal-2 revealed a four exon-three intron organization. a single carbohydrate-binding domain containing all seven important residues for carbohydrate binding was located in the third exon, which formed a car ... | 2017 | 28131766 |
| identification of a gene encoding a membrane-anchored toll-like receptor 5 (tlr5m) in oplegnathus fasciatus that responds to flagellin challenge and activates nf-κb. | toll-like receptor 5 (tlr5) recognizes bacterial flagellin and induces the downstream signaling through the myeloid differentiation primary response gene 88 (myd88) protein to produce proinflammatory cytokines. in this study, we describe a tlr5 membrane form (oftlr5m) and its adaptor protein myd88 (ofmyd88) in rock bream, oplegnathus fasciatus. both oftlr5m (6.7 kb) and ofmyd88 (3.7 kb) genes displayed a quinquepartite structure with five exons and four introns. protein structure of oftlr5m reve ... | 2017 | 28111358 |
| development of pcr method for detecting kudoa iwatai (myxozoa: multivalvulida) from rock bream oplegnathus fasciatus. | we developed a pcr assay targeting the 28s rdna of kudoa iwatai (multivalvulida: myxozoa) and investigated the prevalence of infection in rock bream oplegnathus fasciatus, which is commercially an important aquaculture species in korea, with this assay. detection limit of the pcr assay was 2.5 fg/μl with plasmid dna and 8.6 × 10(3) spores/ml with purified spores, respectively. this pcr assay did not amplify dna of other kudoa species (kudoa septempunctata, kudoa lateolabracis, kudoa thyrsites) t ... | 2017 | 28012028 |