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isoprenoid quinones in the classification of coryneform and related bacteria.menaquinones were the only isoprenoid quinones found in 85 of the 95 coryneform bacteria examined. dihydromenaquinones having nine isoprene units were the main components isolated from corynebacterium bovis, from other glutamic acid-producing strains, and from arthrobacter globiformis and related species. dihydromenaquinones with eight isoprene units were found in brevibacterium linens, the remaining corynebacterium species and strains probably belonging to the genus rhodococcus. tetrahydromenaq ...1979107269
production of beta-lactamase by non-streptomyces actinomycetales.supernatants and whole cells from fermentation broths of micromonospora, nocardia, oerskovia, and other genera of the actinomycetales were examined for the presence of beta-lactamase activity by using the chromogenic cephalosporin 87/312. nearly 60% of the 250 isolates examined produced detectable levels of beta-lactamase. all enzyme preparations were active over a range of ph values from 6.5 to 8.2, with maximum activity occurring between 7.0 and 7.8. the preparations varied in their stability ...1979311614
oerskovia species: rare opportunistic pathogens.the second documented instance of opportunistic infection by member of the genus oerskovia is reported. there is some evidence to suggest that this group of bacteria, which is extensively distributed in the soil, may be more closely associated with man than has hitherto been supposed.1979513112
thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa.acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, corynebacterium haemolyticum, corynebacterium pyogenes or from representatives of the genera arthrobacter, cellulomonas, curtobacterium, kurthia or oerskovia. thin-layer chromatography of whole-organism methanolysates ...1976825611
the structure of the menaquinones with a tetrahydrogenated isoprenoid side-chain.menaquinones with a tetrahydrogenated isoprenoid side-chain of oerskovia turbata and brevibacterium lipolyticum were cyclized to the chromenyl acetate derivatives, which were then submitted to ozonolysis, followed by reduction with dimethylsulfide. the mass-spectrometric analyses of the ozonolysis products revealed the ion peaks at m/e 464 (m+), 449, 422, 407, 267 and 225. these results suggest that the two saturated double bonds are located continuously in the second and third units of the chai ...1977889867
distribution of menaquinones in actinomycetes and corynebacteria.menaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined. dihydromenaquinones having nine isoprene units were the main components isolated from gordona, mycobacterium, corynebacterium bovis, corynebacterium glutamicum and a strain labelled nocardia farcinica, but dihydromenaquinones having eight isoprene units were characteristic of other corynebacterium species and representatives of the 'rhodochrous' complex. tetrahydromenaquinones having six ...1977894261
dextranase activity in oerskovia xanthineolytica. 1976939734
on the chemical structure of menaquinones with the tetrahydrogenated isoprenoid side chain.new menaquinones have been isolated from oerskovia turbata and brevibacterium lipolyticum in the nocardioform and coryneform groups of bacteria, which have been identified to be a series of menaquinones with a tetrahydrogenated isoprenoid chain, designated as menaquinone-9(h4) and menaquinone-8(h4), respectively, by thin layer chromatography, ultraviolet and infrared spectrophotometry and mass spectrometry. the nuclear magnetic resonance spectra of the quinones have revealed that all the two sat ...19761009133
bacterial endocarditis caused by oerskovia turbata.oerskovia turbata is a yellow, motile actinomycete, which before now has only been found in soil and has not been known to cause disease in man or animals. it was isolated from 29 cultures of blood taken during 6 months from an urban pensioner after homograft replacement of his aortic valve. the combination of ampicillin, sulfamethoxazole, and trimethoprim was lethal for o. turbata in vitro; however, antimicrobial therapy alone failed to eradicate the patient's infection. cure was achieved after ...19751200499
molecular structure of rarobacter faecitabidus protease i. a yeast-lytic serine protease having mannose-binding activity.rarobacter faecitabidus protease i (rpi) is a serine protease exhibiting lytic activity toward living yeast cells. rpi is similar to elastase in its substrate specificity and has a lectin-like affinity for mannose. the gene encoding rpi was cloned to elucidate its structure and function. and its nucleotide sequence revealed that it contains an open reading frame encoding a 525-amino acid protein. homology comparison indicated that pre-pro-rpi consists of three domains: (1) an nh2-terminal prepro ...19921339445
oerskovia xanthineolytica and methicillin-resistant staphylococcus aureus in a patient with cirrhosis and variceal hemorrhage. 19921486895
[an axillary abscess produced by oerskovia turbata in an aids patient]. 19911896601
cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods.cellular fatty acid (cfa) compositions of 561 asporogenous, aerobic gram-positive rods were analyzed by gas-liquid chromatography as an adjunct to their identification when grown on blood agar at 35 degrees c. the organisms could be divided into two groups. in the first group (branched-chain type), which included coryneform cdc groups a-3, a-4, and a-5; some strains of b-1 and b-3; "corynebacterium aquaticum"; brevibacterium liquefaciens; rothia dentocariosa; and listeria spp., the rods had siza ...19911899679
primary sequence of the glucanase gene from oerskovia xanthineolytica. expression and purification of the enzyme from escherichia coli.a 2.7-kilobase fragment of dna from oerskovia xanthineolytica containing the gene for a beta-1,3-glucanase has been isolated and its complete nucleotide sequence determined. the sequence was found to contain two large open reading frames. purification of the mature native enzyme and subsequent amino-terminal sequencing defined the glucanase gene in one reading frame which potentially encodes a protein of 548 amino acids. we have expressed this glucanase gene in escherichia coli under control of ...19911985933
grouping of some clinically relevant gram-positive rods by automated fatty acid analysis. diagnostic implications.one hundred and ninety-four strains of aerobically growing gram-positive rods of the genera corynebacterium, actinomyces, arcanobacterium, erysipelothrix, listeria, oerskovia, nocardia, rhodococcus, and of unnamed center for disease control (cdc) groups were checked for cellular fatty acid profiles with the microbial identification system (microbial id, newark, del., usa). in order to obtain unified data usable for the clinical laboratory, 24 or 48 h sheep blood agar cultures were used. it was t ...19912001281
cellular fatty acid composition of oerskovia species, cdc coryneform groups a-3, a-4, a-5, corynebacterium aquaticum, listeria denitrificans and brevibacterium acetylicum.twenty four strains representing eight species of gram positive yellow-pigmented rods (oerskovia turbata, oerskovia xanthineolytica, cdc coryneform groups a-3, a-4, a-5, listeria denitrificans, corynebacterium aquaticum and brevibacterium acetylicum) were divided into two major groups based on the relative amounts of 12 methyltetradecanoate (15:0a) obtained by capillary gas liquid chromatography. o. turbata, o. xanthineolytica, cdc groups a-3 and a-4, l. denitrificans and c. aquaticum were place ...19902124793
separation of yeast asparagine-linked oligosaccharides by high-performance anion-exchange chromatography.oligosaccharides obtained from saccharomyces cerevisiae mannoproteins by digestion with endo-n-acetyl-beta-d-glucosaminidase h were fractionated by anion-exchange chromatography, by elution with 50-100mm naoh without or with a sodium-acetate gradient, and detected with a pulsed amperometric detector (pad). the elution times of homologous oligosaccharides fell on a straight line having a slope characteristic of the structural type. the response of the pad detector per mole of oligosaccharide incr ...19902224897
oerskovia xanthineolytica implicated in peritonitis associated with peritoneal dialysis: case report and review of oerskovia infections in humans.oerskovia species are nocardialike bacteria that have been implicated as pathogens only rarely. these organisms are branched, gram-positive bacilli that are oxidase negative, catalase positive, and non-acid fast. unlike nocardia species, these organisms are motile, do not produce aerial mycelia, and possess a cell wall with large amounts of galactose. colonies are bright yellow and produce branched vegetative hyphae on nutrient agar. a 70-year-old patient undergoing chronic ambulatory peritoneal ...19902229375
preparation and characterization of yeast nuclear extracts for efficient rna polymerase b (ii)-dependent transcription in vitro.we present a reproducible method for the preparation of nuclear extracts from the yeast saccharomyces cerevisiae that support efficient rna polymerase b (ii)-dependent transcription. extracts from both a crude nuclear fraction and percoll-purified nuclei are highly active for site-specific initiation and transcription of a g-free cassette under the adenovirus major late promoter. at optimal extract concentrations transcription is at least 5 times more efficient with the yeast extracts than with ...19902263463
oerskovia infection caused by contaminated home parenteral nutrition solution.only three cases of human infection with oerskovia have been reported. a woman receiving home total parenteral nutrition (tpn) was admitted for catheter-related sepsis caused by oerskovia. she was discharged on an intravenous regimen of vancomycin, but symptoms recurred her first night home, and she was readmitted. cultures of the tpn solution and peripheral blood yielded oerskovia. the patient was successfully treated at home with 5 weeks of intravenous vancomycin therapy (30 mg/kg per day). ho ...19892499294
catheter-related bacteremia caused by oerskovia turbata.a case of broviac catheter-related bacteremia in a 3-year-old boy infected with oerskovia turbata is discussed. the blood cultures of the patient remained positive despite antibiotic therapy, and only with catheter removal was his blood sterilized. the microbiology and antimicrobial susceptibilities of this organism are described.19892715328
design of enzyme systems for selective product release from microbial cells; isolation of a recombinant protein from yeast.the development of expression systems for recombinant proteins and recombinant protein particles that cannot be secreted and that are located in specific cell locations necessitates the development of novel, more selective, techniques for cell disruption. mechanical cell disruption methods do not discriminate the release of the desired product from among a host of other contaminating molecules and cell debris, and they also may damage the protein product. in contrast, the use of lytic enzyme sys ...19883067631
meningitis caused by oerskovia xanthineolytica.in summary, we describe a case of central nervous system infection with o. xanthineolytica in which the infecting microbe probably was engrafted on a ventricular shunt. the bacteria caused a smoldering meningitis that did not respond to penicillin and rifampin despite in vitro sensitivity, presumably because of inadequate cerebrospinal fluid penetration of the penicillin and the recognized difficulty of eradicating bacteria from contaminated shunts. removal of the shunt and continued treatment w ...19883354593
endophthalmitis due to oerskovia xanthineolytica.unilateral endophthalmitis developed in a 47-year-old man following an intraocular eye injury with a metallic foreign body. eventually, oerskovia xanthineolytica was isolated from the vitreous humour. to our knowledge this is the first documented case of endophthalmitis caused by this organism in humans. the clinical course and management of this opportunistic infection are discussed.19873496945
yeast spheroplasts formed by cell wall-degrading enzymes from oerskovia sp.oerskovia sp. produces inducible extracellular enzymes which degrade the walls of various yeasts. yeast spheroplasts are formed from both log- and stationary-phase cells.19724559832
description of oerskovia gen. n. to harbor orskov's motile nocardia.the genus oerskovia is proposed to harbor actinomycetes forming an extensively branched substrate mycelium which usually breaks up into motile elements. cell wall preparations contain major amounts of lysine and galactose. aspartic acid is often present in major amounts. aerial mycelium is not formed. gram reaction and catalase production are positive. the type species is oerskovia turbata comb. n.19705440174
identification key for coryneform bacteria derived by numerical taxonomic studies.six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ...19836413644
rapid cataloging of ribonuclease t1 resistant oligonucleotides from ribosomal rnas for phylogenetic studies.a rapid and simple method of oligonucleotide cataloging for phylogenetic studies is presented. it involves in vitro 5'-32p-labelling of rnase t1 - resistant oligonucleotides of ribosomal 16s rna and finger-printing by high voltage electrophoresis and gradient thinlayer chromatography. oligonucleotide sequences are established by the mobility shift method, using controlled alkali cleavage, high voltage electrophoresis and homochromatography. these procedures facilitate in particular the analysis ...19816790718
isolation of oerskovia xanthineolytica from an aborted equine foetus. 19826889853
lyticase: endoglucanase and protease activities that act together in yeast cell lysis.yeast lytic activity was purified from the culture supernatant of oerskovia xanthineolytica grown on minimal medium with insoluble yeast glucan as the carbon source. the lytic activity was found to consist of two synergistic enzyme activities which copurified on carboxymethyl cellulose and sephadex g-150, but were resolved on bio-gel p-150. the first component was a beta-1,3-glucanase with a molecular weight of 55,000. the k(m) for yeast glucan was 0.4 mg/ml; that for laminarin was 5.9 mg/ml. hy ...19806991473
isolation of protoplasts from aspergillus nidulans conidiospores.protoplasts were prepared from conidiospores of aspergillus nidulans. the mononucleated conidia gave protoplasts of a uniform size, approximately 5-micron diameter, depending on the strain and the stabilizing medium used. conidia were preincubated with 2-deoxy-d-glucose in a minimal medium at 37 degrees c for 3 h. the swollen conidia were collected, resuspended in a buffer containing 0.4 m (nh4)2so4 as stabilizer, and incubated with oerskovia lytic enzymes at 30 degrees c for 3 or 4 h. approxima ...19817016284
analysis of morphogenesis of the nocardioform organism oerskovia xanthineolytica.depending on the nutritional and physicochemical conditions of growth the shape of oerskovia xanthineolytica varied within a broad range. in different exponentially growing cultures five morphological types could be distinguished. in liquid cultures with increasing growth rate oerskovia grew either as rods, filaments or branched filaments, whereas for the agar-microcultures pseudomycelia, but under reduced aeration mycelia were typical. the morphogenetic parameters of each type were determined, ...19817269655
interaction of bacteriophage o2 with strains of the genus oerskovia.bacteriophage o2 multiplies normally on oerskovia turbata imet 47 153. it has a burst size of about 100 p.f.u. per infected cell and a latent period of 100 min at 30 degrees c. on oerskovia xanthineolytica imet 47 383 clear spots were formed after addition of high phage concentrations onto agar top layers. by phase contrast observation, and measurement of the optical density of infected cultures, it was found that the clearing effect on strain imet 47 383 was due to lysis-from-without. phage o2 ...19817293244
identification of some clinical strains of cdc coryneform group a-3 and a-4 bacteria as cellulomonas species and proposal of cellulomonas hominis sp. nov. for some group a-3 strains.cdc coryneform group a-3 and a-4 bacteria were defined by hollis and weaver in 1981, but their taxonomic position is still unclear. by using biochemical and chemotaxonomical methods, four clinical strains belonging to cdc coryneform groups a-3 (n = 2) and a-4 (n = 2) were studied and could be assigned to the genus cellulomonas, resulting in the first description of cellulomonas strains isolated from clinical specimens. cdc coryneform group a-3 and a-4 strains were compared with the type strains ...19957559954
detection of sialidase activity in oerskovia (cellulomonas) turbata.the enzyme n-acylneuraminate glycohydrolase, e.c. 3.2.1.18 (sialidase or neuraminidase) was detected in all five strains of oerskovia (cellulomonas) turbata and in a further strain isolated from clinical material. the detection of sialidase was performed by different methods, i.e. colorimetric determination of liberated chromogen, immunoelectrophoresis and paper chromatography. the oerskovia turbata sialidase is able to cleave different linkages between n-acylneuraminic acid and the carbohydrate ...19957734824
selective release of recombinant protein particles (vlps) from yeast using a pure lytic glucanase enzyme.we have used a pure lytic glucanase enzyme to selectively release recombinant 60 nm protein particles (virus like particles or vlps) from yeast cells. although the protease components of the lytic enzyme complexes were found to degrade the vlps, purified glucanase enzymes from these complexes (derived from cytophaga sp. and oerskovia sp.) produced cell lysis without degradation and released the vlps in the absence of mercaptoethanol, a reducing agent commonly used in cell lysis. the oerskovia gl ...19937764040
production of a bioflocculant by mixed culture.colony groups that form large amounts of slime externally were obtained from activated sludge among phthalate-assimilating microbes. that slime, which r-3 mixed microbes produced externally, had a high level of flocculation activity. r-3 mixed strains, one of such colony groups, efficiently produced a bioflocculant (apr-3) in liquid cultures of production medium, especially that containing starch and glucose (1:1) as carbon sources. identification of this group of bioflocculant-producing microbe ...19947765478
phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of sanguibacter gen.nov.16s rrna gene sequencing studies were performed on some gram-positive coryneform bacteria of unknown taxonomic position isolated from blood and milk of healthy cows. comparative sequence analysis demonstrated that the milk isolates corresponded to oerskovia xanthineolytica whereas those from blood consisted of two distinct, albeit highly related species, within the family cellulomonadaceae. based on the phylogenetic and phenotypic distinctiveness of the blood isolates, it is proposed that they b ...19957765903
oerskovia xanthineolytica bacteremia in an immunocompromised patient with pneumonia.oerskovia species, which are nocardia-like bacteria that have rarely been found to cause human disease, are usually found in association with a foreign body with removal of the infected focus being necessary for cure. we present a case of oerskovia xanthineolytica bacteremia in a patient with metastatic breast cancer, community-acquired pneumonia, and a tunneled subcutaneous central venous catheter. although the actual source of the bacteremia in this case is not proven, this patient's presentat ...19947924223
evaluation of api coryne system for identifying coryneform bacteria.to identify rapidly and accurately coryneform bacteria, using a commercial strip system.19947962633
vana genes in vancomycin-resistant clinical isolates of oerskovia turbata and arcanobacterium (corynebacterium) haemolyticum.we report the cloning and sequencing of vana genes present in the high-level vancomycin- and teicoplanin-resistant clinical isolates oerskovia turbata 892 and arcanobacterium (corynebacterium) haemolyticum 872. the presence of vana was detected by southern blotting and pcr and confirmed by dna sequencing. vana-like sequences were encoded on plasmids of 15 and 20 kb respectively. the a. haemolyticum 872 dna sequence was identical to the published vana sequence of vancomycin-resistant enterococcus ...19958591934
peritonitis caused by oerskovia xanthineolytica in a patient on chronic ambulatory peritoneal dialysis (capd) 19968604720
molecular cloning of a lytic beta-1,3-glucanase gene from oerskovia xanthineolytica llg109. a beta-1,3-glucanase able to selectively permeabilize the yeast cell wall.molecular cloning of the beta giii gene encoding for an endo-beta-1,3-glucanase (beta gl ii) from oerskovia xanthineolytica llg109, a yeast-lytic gram-positive bacterium, has been conducted in order to elucidate its primary sequence and subsequently express it into b. subtilis. this endo-beta-1,3-glucanase exhibits low yeast-lytic activity toward viable s. cerevisiae cells, and it has shown ability to selectively permeabilize the yeast cell wall and release intracellular proteins produced by yea ...19968659924
oerskovia xanthineolytica keratitis. 19968728613
nucleotide sequence of a beta-1,3-glucanase isoenzyme iia gene of oerskovia xanthineolytica ll g109 (cellulomonas cellulans) and initial characterization of the recombinant enzyme expressed in bacillus subtilis.the nucleotide sequence of the betagliia gene, encoding the extracellular beta-1,3-glucanase iia (betagliia) of the yeast-lytic actinomycete oerskovia xanthineolytica ll g109, was determined. sequence comparison shows that the betagliia enzyme has over 80% identity to the betaglii isoenzyme, an endo-beta-1,3-glucanase having low yeast-lytic activity secreted by the same bacterium. the betagliia enzyme lacks a glucan- or mannan-binding domain, such as those observed in beta-1,3-glucanases and pro ...19968755914
oerskovia xanthineolytica infection of a prosthetic joint: case report and review.oerskovia spp. are gram-positive, nocardia-like bacilli which inhabit the soil and rarely cause human infections. previously reported cases of oerskovia infection have been characterized by a nonaggressive course and an association with foreign bodies. we report the first case of a patient with a prosthetic joint infection due to oerskovia xanthineolytica. our patient presented with a prolonged, indolent course and was thought to have aseptic loosening of his prosthesis until the time of surgery ...19968784601
oerskovia turbata and comamonas acidovorans bacteremia in a patient with aids. 19968793408
molecular characterisation of a thermoactive beta-1,3-glucanase from oerskovia xanthineolytica.molecular characterisation of a lytic thermoactive beta-1,3-glucanase from oerskovia xanthineolytica ll-g109 has been performed. a molecular mass of 27 195.6 +/- 1.3 da and an isoelectric point of 4.85 were determined by electrospray mass spectrometry and from its titration curve, respectively. its thermoactivity profile shows it to be a heat-stable enzyme with a temperature optimum of 65 degrees c. the secondary structure content of the protein was estimated by circular dichroism to be approx. ...19968814220
an identification scheme for rapidly and aerobically growing gram-positive rods.an identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ...19968837385
oerskovia xanthineolytica bacteremia in an immunocompromised host: case report and review.oerskovia species, once thought to be nocardiform-like bacteria, have been only rarely associated with human infection. in this report we describe a case of central venous catheter-associated infection that was successfully treated with antibiotics. with the increased use of indwelling devices, these organisms may be more commonly recognized causes of infection. appropriate antibiotic therapy appears to successfully treat oerskovia infection and may decrease the need for removal of some indwelli ...19968852977
bacterial contamination of hydrophilic contact lens solutions marketed in spain.bacterial contamination of hydrophilic contact lens solutions may play an important role in contact lens-associated ocular infections. this study investigated bacterial contamination in 52 different hydrophilic contact lens solutions marketed in spain by 12 different companies. we filtered the entire contents of 5 new, factory-sealed bottles from each of the 52 brands and cultured the fitter on a neutralizing broth plate. bacteria were cultured, isolated, and identified from 29 of the 260 bottle ...19968869983
clinical microbiology of coryneform bacteria.coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ...19978993861
cloning and sequence analysis of genes encoding xylanases and acetyl xylan esterase from streptomyces thermoviolaceus opc-520.three genes encoding two types of xylanases (stx-i and stx-ii) and an acetyl xylan esterase (stx-iii) from streptomyces thermoviolaceus opc-520 were cloned, and their dna sequences were determined. the nucleotide sequences showed that genes stx-ii and stx-iii were clustered on the genome. the stx-i, stx-ii, and stx-iii genes encoded deduced proteins of 51, 35.2, and 34.3 kda, respectively. stx-i and stx-ii bound to both insoluble xylan and crystalline cellulose (avicel). alignment of the deduced ...19979023944
the action of alpha-mannosidase from oerskovia sp. on the mannose-rich o-linked sugar chains of glycoproteins.alpha-mannosidase was isolated from the culture liquid of oerskovia sp. the purified enzyme had a molecular mass of 480 kda and comprises four identical subunits. the enzyme cleaves bonds in side chains of yeast mannan (km = 0.08 mm, k(cat) = 1.02 micromol x min(-1) x mg(-1)) and reveals a low activity towards p-nitrophenyl alpha-d-mannopyranoside. the alpha-mannosidase is a ca2+-dependent enzyme and is inhibited by edta. the enzyme possess no endo-mannosidase activity releasing only mannose in ...19979363781
in vitro susceptibilities of aerobic and facultative non-spore-forming gram-positive bacilli to hmr 3647 (ru 66647) and 14 other antimicrobials.the comparative in vitro activity of the ketolide hmr 3647 (ru 66647) and those of structurally related macrolide-lincosamide-streptogramin compounds (erythromycin, roxithromycin, azithromycin, clarithromycin, josamycin, lincomycin, pristinamycin, and quinupristin-dalfopristin) as well as those of benzylpenicillin, doxycycline, vancomycin, teicoplanin, levofloxacin, and rifapentine against 247 aerobic and facultative non-spore-forming gram-positive bacilli were determined by an agar dilution met ...19989593121
oerskovia xanthineolytica: a new pathogen in bone marrow transplantation.a 53-year-old woman with non-hodgkin lymphoma underwent an autologous bone marrow transplant (bmt). incomplete reconstitution necessitated the use of a long-term central venous catheter. one year after bmt she presented with fever. echocardiography revealed vegetations on the tricuspid valve. gram-positive rods grown from blood cultures and catheter tip were identified as oerskovia xanthineolytica. we report the first case of native valve endocarditis caused by this organism.19989733276
oerskovia xanthineolytica peritonitis: case report and review. 199910462352
phylogenetic and phenotypic diversity of 4-chlorobenzoate-degrading bacteria isolated from soils.twenty numerically dominant 4-chlorobenzoate (4-cba)-degrading bacteria were isolated from agricultural soils. the isolates were able to utilize 4-cba as a sole source of carbon and energy. a total of 65% of the isolates was identified to the species level by fatty acid methyl ester (fame) analysis, and the isolates were strains of micrococcus, pseudomonas, oerskovia, cellulomonas, and arthrobacter species. the chromosomal dna patterns of the isolates obtained by polymerase chain reaction (pcr) ...200010620719
overproduction, purification, and characterization of beta-1,3-glucanase type ii in escherichia coli.an escherichia coli recombinant system produced soluble and full-length beta-1,3-glucanase type ii (bglii) cloned from the yeast-lytic actinomycete oerskovia xanthineolytica. the expression system was designed to produce recombinant bglii with a six-histidine peptide fused to the carboxy end of the protein. the expression level was optimized to produce 30% of total protein of e. coli as the recombinant protein, releasing 75% to the extracellular space. the 43-kda recombinant protein was purified ...200111676595
aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite zootermopsis angusticollis.to demonstrate the occurrence of cellulolytic bacteria in the termite zootermopsis angusticollis.200211849325
re-evaluation of the status of the genus oerskovia, reclassification of promicromonospora enterophila (jáger et al. 1983) as oerskovia enterophila comb. nov. and description of oerskovia jenensis sp. nov. and oerskovia paurometabola sp. nov.phylogenetic analysis of promicromonospora enterophila indicates that this taxon clusters with cellulomonas species, adjacent to cellulomonas turbata (basonym oerskovia turbata). 16s rdna analysis, dna-dna reassociation, riboprinting, peptidoglycan analysis and determination of phenotypic properties of various strains of p. enterophila and c turbata reveal that they form a cluster that can be distinguished unambiguously from other cellulomonas species by morphology, amino acid composition of the ...200212148614
oerskovia xanthineolytica bacteremia in an immunocompromised patient without a foreign body. 200312709844
enzymatic synthesis of 4-methylumbelliferyl (1-->3)-beta-d-glucooligosaccharides-new substrates for beta-1,3-1,4-d-glucanase.the transglycosylation reactions catalyzed by beta-1,3-d-glucanases (laminaranases) were used to synthesize a number of 4-methylumbelliferyl (meumb) (1-->3)-beta-d-gluco-oligosaccharides having the common structure [beta-d-glcp-(1-->3)](n)-beta-d-glcp-meumb, where n=1-5. the beta-1,3-d-glucanases used were purified from the culture liquid of oerskovia sp. and from a homogenate of the marine mollusc spisula sachalinensis. laminaran and curdlan were used as (1-->3)-beta-d-glucan donor substrates, ...200312829391
oerskovia xanthineolytica endocarditis in a renal transplant patient: case report and review of the literature.oerskovia species were, until recently, only rarely associated with human disease. this gram-positive bacillus can be easily misidentified as a diphtheroid, a common contaminant in blood cultures. there have been 17 reports of invasive oerskovia infection in immunocompromised hosts. we report a case of oerskovia xanthineolytica endocarditis in a renal transplant patient and review the microbiologic and clinical characteristics of this potential pathogen.200314987205
molecular epidemiologic evaluation of endocarditis due to oerskovia turbata and cdc group a-3 associated with contaminated homograft valves.oerskovia turbata is an unusual bacterial cause of endocarditis and septicemia in immunocompromised patients. in this study, we compared 12 isolates from a 1975 medical center cluster, 11 originally identified as o. turbata (four from the blood of a homograft aortic valve-associated endocarditis patient and seven from contaminated homograft valves) and one cdc group a-3 strain from the blood of a second endocarditis patient with fatal outcome, with eight control strains from unrelated locations. ...200415184426
equine abortion and premature birth associated with cellulosimicrobium cellulans infection.during the 2002 and 2003 foaling seasons, cellulosimicrobium (cellumonas) cellulans (formerly oerskovia xanthineolytica) was the principal microorganism isolated from fetal tissues or placentas from cases of equine abortion, premature birth, and term pregnancies. significant pathologic findings included chronic placentitis and pyogranulomatous pneumonia. in addition, microscopic and macroscopic alterations in the allantochorion from 4 of 7 cases of placentitis were similar to those caused by cro ...200415305747
[coryneform bacteria: their clinical significance and resistance patterns during a three-year study].we report data concerning our experience during three years (1998-2001) about isolation, identification and susceptibility towards antimicrobial agents of coryneform bacteria in infections of hospitalized/at risk patients. we isolated 54 corynebacterium spp., with prevalence of c. striatum (8 strains) and c. amycolatum (7 strains), and 1 strain of oerskovia spp. and 1 strain of actinomyces neuii. 31 strains were isolated from the "exit-site" and 6 from peritoneal fluid of capd patients. vancomyc ...200415316299
[the ability of saprotrophic bacteria isolated from natural habitats to lyse yeasts].more than 600 bacterial strains isolated from different horizons of steppe biogeocenoses and zoogenous loci (diplopod intestines and feces) were tested for the ability to lyse yeast cell walls. about half of the strains that were isolated from biotopes with active degradation of plant debris (steppe litters and diplopod intestines and feces) were found to possess yeast-lytic activity. most of the yeast-lytic strains belonged to the genera streptomyces, promicromonospora, oerskovia, and agromyces ...200415521184
identification of a chitinase-producing bacterium c4 and histopathologic study on locusts.in order to develop the potential of chitinase-producing micro-organisms as biocontrol agents for insect pests, five chitinase-producing bacterial strains (c1, c2, c3, c4 and c5) previously isolated from soil samples were chosen to infect grassland locusts. the data showed that the mortality rate of locusts fed with strain c4 was significantly higher than that of other groups, and its pathogenicity was confirmed by koch's law. midgut tissues of locusts infected with c4 were examined with a light ...200515619732
phenotypic and genetic characterization of clinical isolates of cdc coryneform group a-3: proposal of a new species of cellulomonas, cellulomonas denverensis sp. nov.cdc coryneform group a-3 bacteria are rare human pathogens. in this study, six group a-3 isolates (two from blood, one from cerebrospinal fluid, and one each from homograft valve, lip wound, and pilonidal cyst) were compared to the type strains of phenotypically related organisms, cellulomonas fimi, cellulomonas hominis, oerskovia turbata, and sanguibacter suarezii, and characterized by phenotypic, chemotaxonomic, and genotypic studies. dna-dna reassociation analysis identified two genomic group ...200515814993
production and ecological significance of yeast cell wall-degrading enzymes from oerskovia.motile actinomycetes capable of degrading walls of viable yeast cells were isolated from soil and identified as oerskovia xanthineolytica. a lytic assay based on susceptibility of enzyme-treated cells to osmotic shock was developed, and 10 of 15 strains of o. xanthineolytica, oerskovia turbata, and nonmotile oerskovia- like organisms from other collections were found to possess yeast lytic activities. all lytic strains produced laminaranase and alpha-mannanase, but the amounts, determined by red ...197816345321
transformation of adenine to 8-hydroxyadenine by strains of oerskovia xanthineolytica.the 8-hydroxy derivative of adenine (6-amino-1,7-dihydro-8h-purin-8-one) is produced from adenine by two oerskovia xanthineolytica strains. this transformation by a microorganism has not been reported previously. no novel products of dissimilation of xanthine (3,7-dihydro-1h-purine-2,6-dione) or hypoxanthine (1,7-dihydro-6h-purin-6-one) were found. xanthine was oxidized to uric acid, but intermediates in the breakdown of hypoxanthine could not be demonstrated.198216345943
revisiting the cellulosimicrobium cellulans yeast-lytic beta-1,3-glucanases toolbox: a review.cellulosimicrobium cellulans (also known with the synonyms cellulomonas cellulans, oerskovia xanthineolytica, and arthrobacter luteus) is an actinomycete that excretes yeast cell wall lytic enzyme complexes containing endo-beta-1,3-glucanases [ec 3.2.1.39 and 3.2.1.6] as key constituents. three genes encoding endo-beta-1,3-glucanases from two c. cellulans strains have been cloned and characterised over the past years. the betaglii and betagliia genes from strain dsm 10297 (also known as o. xanth ...200616545129
characterization of clinical isolates previously identified as oerskovia turbata: proposal of cellulosimicrobium funkei sp. nov. and emended description of the genus cellulosimicrobium.taxonomic studies were performed on 13 clinical isolates (ten of which were epidemiologically related) that had been previously identified as oerskovia turbata. comparative phylogenetic analysis, based on 16s rrna gene sequences, indicated that the isolates are closely related to cellulosimicrobium cellulans with sequence similarity values ranging from 99.5 to 99.8 %. chemotaxonomic results (fatty acid profiles and menaquinones) supported the inclusion of these isolates in the genus cellulosimic ...200616585698
[meningitis caused by oerskovia xanthineolytica].oerskovia species are nocardia-like bacteria that have been only and rarely associated with human infections. in this report, a meningitis case caused by oerskovia xanthineolytica was presented. a 44 years old male patient who had experienced ventriculo-peritoneal shunt (v-p shunt) due to hydrocephaly, developed meningitis. o. xanthineolytica was isolated from three of the cerebrospinal fluid (csf) samples and v-p shunt cultures. identification of the isolate was performed by phoenix systemtm (n ...200616775964
clearance of cellulosimicrobium cellulans bacteremia in a child without central venous catheter removal.cellulosimicrobium cellulans (formerly known as oerskovia xanthineolytica) rarely causes human infection. infections have been reported in immunocompromised hosts or in patients with foreign bodies, such as catheters, where treatment has generally involved removal of the foreign body. we report on a case in which the organism was isolated in multiple blood cultures from a 13-year-old male. after initial therapy failed, treatment with vancomycin and rifampin resulted in infection clearance withou ...200616825406
potential misidentification of a new exiguobacterium sp. as oerskovia xanthineolytica isolated from blood culture. 200616872001
community structure of actively growing bacterial populations in plant pathogen suppressive soil.the bacterial community in soil was screened by using various molecular approaches for bacterial populations that were activated upon addition of different supplements. plasmodiophora brassicae spores, chitin, sodium acetate, and cabbage plants were added to activate specific bacterial populations as an aid in screening for novel antagonists to plant pathogens. dna from growing bacteria was specifically extracted from the soil by bromodeoxyuridine immunocapture. the captured dna was fingerprinte ...200716944345
oerskovia turbata and myroides species: rare isolates from a case of acalculus cholecystitis.here we report a case of acalculus cholecystitis, which presented with features of obstructive jaundice of one-week duration. the patient underwent cholecystectomy and bile grew a mixed culture of oerskovia turbata and myroides spp. being a rare isolate, characteristic features of the former are described in this report. the patient recovered without any complication.200717901658
kinetics of enzymatic lysis and disruption of yeast cells: i. evaluation of two lytic systems with different properties.many microorganisms produce enzymes which lyse the walls of yeasts, fungi, and bacteria. the proportions of different enzyme activities present in the lytic system, their action patterns, synergism, and dependence on inhibitors, constitute the activity profile of the lytic system. taken together, the activity profile and process conditions for lysis determine the reaction rate and the distribution of products from lysis of any given type of cells. kinetics of glucan hydrolysis, proteolysis, and ...198718581424
kinetics of enzymatic lysis and disruption of yeast cells: ii. a simple model of lysis kinetics.a simple two-step model is proposed to describe the kinetics of the two lytic systems examined in the preceding article. the model predicts concentrations of yeast solids, soluble proteins, peptides, and carbohyrates. in the first reaction step, yeast cell mass is solubilized; in the second, the released protein can be hydrolyzed to peptides. kinetics for both yeast lysis and the subsequent protein breakdown are based on michaelis-menten expressions. terms have been included for competitive inhi ...198718581425
continuous-culture studies of synthesis and regulation of extracellular beta(1-3) glucanase and protease enzymes from oerskovia xanthineolytica.this article describes the synthesis and regulation of beta(1-3)glucanase and protease enzymes from the cell lytic system of oerskovia xanthineolytica ll-g109 in continuous culture using different concentrations of carbon source (glucose) and inducer (glucan). these two enzyme activities are the main components of a lytic system capable of lysing and disrupting whole yeast cells; it is subject to catabolite repression by glucose and is induced by yeast glucan. peaks of beta(1-3)glucanase and pro ...198718581450
pyogenic flexor tenosynovitis associated with cellulosimicrobium cellulans.cellulosimicrobium cellulans, formerly known as oerskovia xanthineolytica, is a rare human pathogen, often in association with a foreign body. a case of pyogenic flexor tenosynovitis associated with c. cellulans in an immunocompetent boy is described, underlining the importance of prompt surgical and microbiologic evaluation.200818832122
isolation and characterization of actinomycetes from healthy goat faeces.to isolate and characterize actinomycetes with probiotic activities from healthy goat faeces.200919552775
paraoerskovia marina gen. nov., sp. nov., an actinobacterium isolated from marine sediment.a gram-positive-staining, facultatively anaerobic bacterial strain, ctt-37t, was isolated from a marine sediment sample collected from tottori city, located on the shore of the sea of japan. a 16s rrna gene sequence comparison indicated that the isolate represents a novel clade that clusters with members of the families cellulomonadaceae and sanguibacteraceae. strain ctt-37t shared maximum 16s rrna gene sequence similarity of 96.4% with oerskovia paurometabola dsm 14281t and 96.2% with oerskovia ...200919605707
isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in xishuangbanna, china.endophytic actinobacteria are relatively unexplored as potential sources of novel species and novel natural products for medical and commercial exploitation. xishuangbanna is recognized throughout the world for its diverse flora, especially the rain forest plants, many of which have indigenous pharmaceutical histories. however, little is known about the endophytic actinobacteria of this tropical area. in this work, we studied the diversity of actinobacteria isolated from medicinal plants collect ...200919648362
luteimicrobium subarcticum gen. nov., sp. nov., a new member of the suborder micrococcineae.a novel gram-stain-positive bacterium, designated r19-04(t), was isolated from a soil sample from rishiri island, japan, and its taxonomic position was investigated by a polyphasic approach. cells of strain r19-04( t) exhibited a rod-coccus cycle. the peptidoglycan type of the isolate was a4alpha; lysine was the diagnostic diamino acid. the predominant menaquinone was mk-8(h(2)) and the major fatty acid was anteiso-c(15 : 0). the polar lipids were diphosphatidylglycerol and phosphatidylglycerol. ...201019656927
study of thermostable chitinases from oerskovia xanthineolytica ncim 2839.the mesophilic organism, oerskovia xanthineolytica ncim 2839, was adapted to grow at moderate thermophilic temperatures. at these elevated temperatures, it was found to produce two thermostable chitinases--c1 and c2. these were purified by ion exchange chromatography using deae cellulose. the chitinases c1 and c2 were found to be stable in a ph range from 3.0 to 9.0 with 7.5 and 8.0 being the optimum ph, respectively. the optimum temperatures of the activities of c1 and c2 were 50 and 55 degrees ...201020091303
the diversity and anti-microbial activity of endophytic actinomycetes isolated from medicinal plants in panxi plateau, china.traditional chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. the endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. panxi plateau in south-west sichuan in china with its unique geographical and climatological characteristics is a habitat of a great variety of medicinal plants. in this study, 560 endophytic actinomycetes were isola ...201120567975
isolation of tn1546-like elements in vancomycin-resistant enterococcus faecium isolated from wood frogs: an emerging risk for zoonotic bacterial infections to humans.isolation and characterization of vancomycin-resistant enterococci (vre), mainly enterococcus faecium, from the faecal pellet of wood frogs (rana sylvatica).201020880145
efficient conversion from polysialogangliosides to monosialotetrahexosylganglioside using oerskovia xanthineolytica yz-2.a new sialidase-producing strain isolated from soil was identified as oerskovia xanthineolytica yz-2. sialidase was produced when oerskovia xanthineolytica yz-2 was exposed to polysialogangliosides. the sialidase of oerskovia xanthineolytica yz-2 hydrolyzed sialic acid linkages in polysialogangliosides, and released monosialotetrahexosylganglioside (gm1). the sialidase had the capability of product specificity because it did not attack the sialic acid linkage in gm1. therefore, oerskovia xanthin ...201021153421
complete genome sequence of sanguibacter keddieii type strain (st-74).sanguibacter keddieii is the type species of the genus sanguibacter, the only genus within the family of sanguibacteraceae. phylogenetically, this family is located in the neighborhood of the genus oerskovia and the family cellulomonadaceae within the actinobacterial suborder micrococcineae. the strain described in this report was isolated from blood of apparently healthy cows. here we describe the features of this organism, together with the complete genome sequence, and annotation. this is the ...200921304646
[first case of peritoneal infection due to oerskovia turbata (cellulosimicrobium funkei)]. 201121461020
chitinase production in solid-state fermentation from oerskovia xanthineolytica ncim 2839 and its application in fungal protoplasts formation.the present study reports the economic production of thermostable chitinase production from oerskovia xanthineolytica ncim 2839 by solid-state fermentation (ssf) technique and its application in fungal protoplasts formation. the oerskovia xanthineolytica ncim 2839 was found to produce thermostable chitinase 148 u g(-1) of solid substrate in ssf using wheat bran with colloidal chitin as base. protoplasts of a. niger were formed by using crude chitinase produced in ssf and formed protoplasts were ...201121744286
[spondylodiscitis].the authors present a clinical case of a female child, aged 23 months, previously healthy that 24 hours after falling down started to refuse walking and complained about back pain. she never had fever. she felt better with nonsteroidal antiinflammatory drugs, restarting the symptoms, two days after therapy suppression. at admission on hospital she presented inability to flex the lower back and loss of lumbar lordosis, being normal the remaining physical examination. there was a raise of erythroc ...200322226217
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