| parity between kinetoplast dna and mini-exon gene sequences supports either clonal evolution or speciation in trypanosoma rangeli strains isolated from rhodnius colombiensis, r. pallescens and r. prolixus in colombia. | trypanosoma rangeli are kinetoplastid protozoa which have been largely recognized and defined in several latin american countries in relation to t. cruzi, because the two trypanosome species are frequently found in mixed infections in triatominae vectors, humans and a variety of wild and domestic mammals. we report the molecular characterization of 18 t. rangeli strains isolated from the salivary glands of naturally infected rhodnius colombiensis, r. pallescens and r. prolixus by using two indep ... | 2003 | 12797971 |
| detection of trypanosoma cruzi and trypanosoma rangeli infection in triatomine vectors by amplification of the histone h2a/sire and the sno-rna-c11 genes. | trypanosoma rangeli is non pathogenic for humans but of important medical and epidemiological interest because it shares vertebrate hosts, insect vectors, reservoirs and geographic areas with t. cruzi, the etiological agent of chagas disease. therefore, in this work, we set up two pcr reactions, tch2af/r and trfr2, to distinguish t. cruzi from t. rangeli in mixed infections of vectors based on amplification of the histone h2a/sire and the small nucleolar rna cl1 genes, respectively. both pcrs we ... | 2007 | 17384816 |
| [comparison of feeding and defecation patterns of rhodnius colombiensis and rhodnius prolixus(hempitera, reduviidae, triatominae) under laboratory conditions]. | rhodnius colombiensis occasionally comes into human dwellings and consequently its role as an important potential vector in the transmission of american trypanosomiasis has been suggested. | 2007 | 18154250 |
| [comparison of the life cycles of rhodnius colombiensis moreno, jurberg & galvão, 1999 and r. prolixus stal, 1872 (hemiptera, reduviidae, triatominae) under laboratory conditions]. | rhodnius colombiensis is a sylvatic triatomine associated with wine palm trees (attalea butyracea) in the high basin of the magdalena river (colombia). the frequent invasion of these vectors into human dwellings and the high prevalences of natural infection with trypanosoma cruzi of these insects suggest an important role in the transmission of chagas disease. | 2007 | 18154252 |
| [electrophoretic patterns of salivary hemeproteins (nitrophorines) of rhodnius colombiensis and r. prolixus (hemiptera, reduviidae, triatominae)]. | salivary hemeprotein electrophoresis in starch gels is a recent technique used for differentiation of rhodnius species with great phenotypic similarity. furthermore, populations of the same species can be differentiated from geographically separated locales. of the 15 described rhodnius species in latin america, at least eight have been reported in colombia. | 2007 | 18154254 |
| trypanosoma rangeli genotypes association with rhodnius prolixus and r. pallescens allopatric distribution in central america. | previous kdna polymorphism-based reports have revealed the existence of two trypanosoma rangeli genotypes (kp1+ and kp1-): sl and ssu rrna gene polymorphism-based studies have revealed that five genotypes (a-e) are distributed throughout different latin-american countries. some evidence has shown that the genotypes' biogeographical distribution is associated with sympatric rhodnius species. 12 t. rangeli isolates from humans and reservoirs from el salvador, guatemala, honduras, costa rica and pa ... | 2009 | 19778637 |
| sequence analysis of the spliced-leader intergenic region (sl-ir) and random amplified polymorphic dna (rapd) of trypanosoma rangeli strains isolated from rhodnius ecuadoriensis, r. colombiensis, r. pallescens and r. prolixus suggests a degree of co-evolution between parasites and vectors. | spliced leader intergenic region (sl-ir) sequences from 23 trypanosoma rangeli strains isolated from the salivary glands of rhodnius colombiensis, r. ecuadoriensis, r. pallescens and r. prolixus and two human strains revealed the existence of 4 genotypes with ca, gt, ta, att and gtat microsatellite repeats and the presence of insertions/deletions (indel) and single nucleotide polymorphism (snp) characterizing each genotype. the strains isolated from the same vector species or the same rhodnius e ... | 2011 | 21718675 |
| chromosomal evolution in the pallescens group (hemiptera, triatominae). | rhodnius colombiensis, in conjunction with r. pallescens and r. ecuadoriensis, forms the monophyletic pallescens group. cytogenetic analyses of these closely related species would further our understanding of the taxonomy and evolution of this group. in this study, r. colombiensis was cytogenetically analyzed, and the results were compared with cytogenetic data from other species of the pallescens group, particularly their chromosomal evolution. we found that this triatomine has heteropycnotic b ... | 2015 | 26505416 |
| characterization of the external female genitalia of six species of triatominae (hemiptera: reduviidade) by scanning electron microscopy. | by macroscopic and microscopic dorsal side observation, it was noted that the ix and x segments of two species each of panstrongylus and triatoma terminate in an elongated way, whereas they terminate abruptly in the two species of rhodnius. scanning observation of the dorsal, ventral, lateral and posterior sides of the female genitalia of panstrongylus herreri, panstrongylus megistus, rhodnius colombiensis, rhodnius prolixus, triatoma infestans and triatoma vitticeps revealed that these six spec ... | 2010 | 20512241 |
| comparative descriptions of eggs from three species of rhodnius (hemiptera: reduviidae: triatominae). | the authors describe and compare the morphological and ultrastructural characteristics of eggs from the three most recent described species of the genus rhodnius stål, 1859, which have not previously been studied. these species are rhodnius colombiensis (mejia, galvão & jurberg 1999), rhodnius milesi (carcavallo, rocha, galvão & jurberg 2001) and rhodnius stali (lent, jurberg & galvão 1993). the results revealed that there are similarities in the exochorial architecture of optical microscopy and ... | 2009 | 20027470 |
| molecular characterisation of trypanosoma rangeli strains isolated from rhodnius ecuadoriensis in peru, r. colombiensis in colombia and r. pallescens in panama, supports a co-evolutionary association between parasites and vectors. | we present data on the molecular characterisation of strains of trypanosoma rangeli isolated from naturally infected rhodnius ecuadoriensis in peru, from rhodnius colombiensis, rhodnius pallescens and rhodnius prolixus in colombia, and from rhodnius pallescens in panama. strain characterisation involved a duplex pcr with s35/s36/kp1l primers. mini-exon gene analysis was also carried out using trint-1/trint-2 oligonucleotides. kdna and mini-exon amplification indicated dimorphism within both dna ... | 2005 | 15639744 |
| differentiation and genetic analysis of rhodnius prolixus and rhodnius colombiensis by rdna and rapd amplification. | domiciliated rhodnius prolixus and sylvatic r. colombiensis were analyzed in order to confirm their genetic divergence and verify the risk that the latter represents in the domiciliation process, and to provide tools for identifying the sources of possible reinfestation by triatomines in human dwellings allowing control programs to be undertaken. comparison of random amplified polymorphic dna amplification patterns and cluster analysis suggests reproductive discontinuity between the two species. ... | 2001 | 11784920 |
| amplification of a specific repetitive dna sequence for trypanosoma rangeli identification and its potential application in epidemiological investigations. | trypanosoma rangeli can infect humans as well as the same domestic and wild animals and triatomine vectors infected by trypanosoma cruzi in central and south america. this overlapping distribution complicates the epidemiology of american trypanosomiasis due to the cross-reactivity between t. rangeli and t. cruzi antigens and the presence of conserved dna sequences in these parasites. we have isolated a t. rangeli-specific dna repetitive element which is represented in approximately 103 copies pe ... | 2000 | 11162365 |