| proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus. | members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ... | 2001 | 11491354 |
| heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16s-23s intergenic ribosomal rna polymorphisms. | peptostreptococci are gram-positive, strictly anaerobic bacteria which, although regarded as members of the commensal human microflora, are also frequently isolated from sites of clinical infection. the study of this diverse group of opportunist pathogens has been hindered by an inadequate taxonomy and the lack of a valid identification scheme. recent re-classification of the peptostreptococcus family into five distinct genus groups has helped to clarify the situation. however, this has been on ... | 2002 | 12448679 |
| rapid identification of gram-positive anaerobic coccal species originally classified in the genus peptostreptococcus by multiplex pcr assays using genus- and species-specific primers. | here, a rapid and reliable two-step multiplex pcr assay for identifying 14 gram-positive anaerobic cocci (gpac) species originally classified in the genus peptostreptococcus (anaerococcus hydrogenalis, anaerococcus lactolyticus, anaerococcus octavius, anaerococcus prevotii, anaerococcus tetradius, anaerococcus vaginalis, finegoldia magna, micromonas micros, peptostreptococcus anaerobius, peptoniphilus asaccharolyticus, peptoniphilus harei, peptoniphilus indolicus, peptoniphilus ivorii and pepton ... | 2003 | 12855723 |
| [role of obligate anaerobes in infections in hospitalized patients and therapeutic options]. | monitoring of prevalence and antibiotic susceptibility of strictly anaerobic bacteria, causing infections in hospitalized patients, constitutes a part of a program for prudent use of antibiotics. the aim of the study was to assess prevalence of strictly anaerobic bacteria in patients hospitalized in a tertiary care hospital in 2001-2002 with reference to empiric antibiotic therapy. the most common gram-positive bacteria were clostridium difficile--27.7%, peptostreptococcus spp. and peptoniphilus ... | 2005 | 16134392 |
| in vitro activity of oritavancin (ly333328), vancomycin, clindamycin, and metronidazole against clostridium perfringens, propionibacterium acnes, and anaerobic gram-positive cocci. | using an agar dilution method, we determined the in vitro activity of oritavancin, vancomycin, clindamycin and metronidazole against 114 unique clinical isolates of gram-positive anaerobes. mic(90)s (microg/ml) for oritavancin were as follows: clostridium perfringens 1.0, propionibacterium acnes 0.25, peptostreptococcus anaerobius 0.25, peptoniphilus asaccharolyticus 0.5, finegoldia magna 0.25, micromonas. micros 0.25, and anaerococcus prevotii 0.25. on a weight basis, oritavancin is slightly mo ... | 2005 | 16701537 |
| in vitro activities of dalbavancin and 12 other agents against 329 aerobic and anaerobic gram-positive isolates recovered from diabetic foot infections. | tests of dalbavancin's in vitro activity against 209 aerobic and 120 anaerobic isolates from pretreatment diabetic foot infections showed an mic(90) of < or =0.125 microg/ml against methicillin-susceptible staphylococcus aureus (mssa), methicillin-resistant s. aureus (mrsa), and 120 anaerobes (clostridium perfringens, other clostridia, peptoniphilus asaccharolyticus, finegoldia magna, and anaerococcus prevotii), compared to respective mic(90)s for mssa and mrsa of 0.5 and 1 microg/ml for vancomy ... | 2006 | 16870792 |
| in vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections. | against 443 aerobic and anaerobic bacteria isolated from diabetic foot infections, ceftobiprole mics (microg/ml) at which 90% of the isolates tested were inhibited were as follows: methicillin-resistant staphylococcus aureus, 1; methicillin-susceptible s. aureus and staphylococcus lugdunensis, 0.5; anaerococcus prevotii, 0.125; finegoldia magna, 0.5; peptoniphilus asaccharolyticus, 1; peptostreptococcus anaerobius, 4; escherichia coli and enterobacter species, 0.125; klebsiella species, 2; and p ... | 2006 | 16982780 |
| peptoniphilus gorbachii sp. nov., peptoniphilus olsenii sp. nov., and anaerococcus murdochii sp. nov. isolated from clinical specimens of human origin. | three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. phenotypic and genotypic data demonstrate that these organisms are distinct, and each group represents a previously unknown subline within clostridium cluster xiii. two groups are most closely related to peptoniphilus harei in the genus peptoniphilus, and the other group is most closely related to anaerococcus lactolyticus in the genus anaerococ ... | 2007 | 17428937 |
| clostridium tertium isolated from gas gangrene wound; misidentified as lactobacillus spp initially due to aerotolerant feature. | clostridium tertium has been increasingly reported as a human pathogen. this organism is an aerotolerant gram-positive rod that is often mistaken for other organisms, such as lactobacillus or bacillus species. we describe a case of a patient with a history of intravenous drug use presenting to ucla-olive view medical center with gas gangrene of both upper extremities. the organism was initially misidentified as a lactobacillus species on aerobic culture plates. however, terminal spore formation ... | 2007 | 17446094 |
| development of 16s rrna-based probes for the identification of gram-positive anaerobic cocci isolated from human clinical specimens. | fluorescent probes targeted at 16s rrna were designed for peptostreptococcus anaerobius and peptostreptococcus stomatis (pana134), parvimonas micra (pamic1435), finegoldia magna (fmag1250), peptoniphilus asaccharolyticus (pnasa1254), peptoniphilus ivorii (pnivo731), peptoniphilus harei (pnhar1466), anaerococcus vaginalis (avag1280) and anaerococcus lactolyticus (alac1438), based on the 16s rrna sequences of reference strains and 88 randomly chosen clinical isolates. these strains were also used ... | 2007 | 17714522 |
| survey of bacterial diversity in chronic wounds using pyrosequencing, dgge, and full ribosome shotgun sequencing. | chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. these bacterial populations cooperate to promote their own survival and the chronic nature of the infection. few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. the use of 3 separate16s-based molecular amplifications followed by pyrosequencing, shotgun sanger sequencing, and denatur ... | 2008 | 18325110 |
| the lower genital tract microbiota in relation to cytokine-, slpi- and endotoxin levels: application of checkerboard dna-dna hybridization (cdh). | in the present study the lower genital tract microbiota in asymptomatic fertile women (n=34) was identified and quantified by culturing vaginal secretions. also, vaginal and cervical samples were analyzed by a semiquantitative checkerboard dna-dna hybridization technique (cdh) based on genomic probes prepared from 13 bacterial species (bacteroides ureolyticus, escherichia coli, fusobacterium nucleatum, gardnerella vaginalis, mobiluncus curtisii ss curtisii, prevotella bivia, prevotella disiens, ... | 2008 | 18397461 |
| quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems. | transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ... | 2008 | 18579722 |
| relationship of specific vaginal bacteria and bacterial vaginosis treatment failure in women who have sex with women. | bacterial vaginosis frequently persists after treatment. the role of newly defined bacterial vaginosis-associated bacteria (bvab), which have a specificity for this condition of 97% or greater, has not been assessed. | 2008 | 18591634 |
| polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded flx amplicon pyrosequencing (btefap). | diabetic extremity ulcers are associated with chronic infections. such ulcer infections are too often followed by amputation because there is little or no understanding of the ecology of such infections or how to control or eliminate this type of chronic infection. a primary impediment to the healing of chronic wounds is biofilm phenotype infections. diabetic foot ulcers are the most common, disabling, and costly complications of diabetes. here we seek to derive a better understanding of the pol ... | 2008 | 18833331 |
| anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste. | a strictly anaerobic, mesophilic and aminolytic strain (wn036(t)) was isolated from a methanogenic reactor treating waste from cattle farms. cells were gram-positive cocci, often occurred in pairs and were non-motile. although spore formation was not confirmed by microscopic observation of cells, the strain produced thermotolerant cells. the optimum temperature for growth was 35-37 degrees c and the optimum ph was 6.7. oxidase, catalase and nitrate-reducing activities were negative. the novel st ... | 2009 | 19643871 |
| bacterial diversity in surgical site infections: not just aerobic cocci any more. | to evaluate the microbial diversity in chronic surgical site infections (ssis). | 2009 | 19862869 |
| the effects of circumcision on the penis microbiome. | circumcision is associated with significant reductions in hiv, hsv-2 and hpv infections among men and significant reductions in bacterial vaginosis among their female partners. | 2010 | 20066050 |
| identification of rhesus macaque genital microbiota by 16s pyrosequencing shows similarities to human bacterial vaginosis: implications for use as an animal model for hiv vaginal infection. | the composition of the lower genital tract microbiota in women is believed to affect the risk of sexually acquiring hiv. since macaque genital microbiota could similarly impact vaginal infection with siv we identified microbiota in 11 rhesus macaques using multitag pyrosequencing of the 16s rrna gene. the microbiota was polymicrobial with a median of nine distinct bacterial taxa per macaque (range 3-16 taxa, each constituting 1% or more of the sequences). taxa frequently found included peptoniph ... | 2010 | 20156101 |
| molecular characterization of the polymicrobial flora in chronic rhinosinusitis. | conventional cultures have implicated staphylococcus aureus (sa) and coagulase-negative staphylococcus (cns) as principal pathogens in chronic rhinosinusitis (crs). these results are questioned by recent studies in which molecular probes implicate haemophilus influenzae instead. | 2010 | 20211106 |
| crystallization and preliminary structural analyses of glutamate dehydrogenase from peptoniphilus asaccharolyticus. | glutamate dehydrogenase (ec 1.4.1.2-4) from peptoniphilus asaccharolyticus has been expressed as a selenomethionine-derivatized recombinant protein and diffraction-quality crystals have been grown that are suitable for structure determination. preliminary structural analyses indicate that the protein assembles as a homohexameric enzyme complex in solution, similar to other bacterial and mammalian enzymes to which its sequence identity varies between 25 and 40%. the structure will provide insight ... | 2010 | 20445250 |
| a comparison of bacterial composition in diabetic ulcers and contralateral intact skin. | an extensive portion of the healthcare budget is allocated to chronic human infection. chronic wounds in particular are a major contributor to this financial burden. little is known about the types of bacteria which may contribute to the chronicity, biofilm and overall bioburden of the wound itself. in this study we compare the bacteriology of wounds and associated intact skin. wound and paired intact skin swabs (from a contralateral location) were collected. the bacterial diversity was determin ... | 2010 | 20461221 |
| peptoniphilus methioninivorax sp. nov., a novel gram-positive anaerobic coccus isolated from retail ground beef. | strain nrrl b-23883 was isolated from retail ground beef as part of a study on the genetic diversity of clostridium perfringens. the strain was found to be a strictly anaerobic, gram- positive coccus that was able to utilize peptone as a sole carbon source. subsequent to sequencing the 16s ribosomal rna gene, it was found that the strain was closely related to species within the genera peptoniphilus and anaerosphaera, but it was substantially different from the closest recognized species by near ... | 2010 | 20817843 |
| molecular analysis of bacterial communities in uranium ores and surrounding soils from banduhurang open cast uranium mine, india: a comparative study. | bacterial community structure of heavy metal rich- uranium ores and surrounding soils was explored using 16s rrna gene based clone library analysis and denaturing gradient gel electrophoresis (dgge) to provide baseline microbial diversity data on autochthonous communities. sequence analysis of major ribotypes and/or dgge bands revealed proteobacteria and acidobacteria as the two most frequently present bacterial phyla across the samples, although relative abundance of each phyla and identity of ... | 2011 | 21308598 |
| anti-anaerobic activity of serum from patients treated with tigecycline for skin/soft tissue infections. | to gain additional data concerning the anti-anaerobic activity of tigecycline in serum, we analyzed blood samples from six patients with a complicated skin/soft tissue infection who were receiving iv tigecycline 50 mg every 12 h. venous blood samples were obtained after multiple doses of tigecycline at 1, 6 and 12 h after the initiation of a 1 h iv infusion. sera from these samples were tested to determine serum inhibitory and bactericidal activity over time against 4 anaerobic bacteria (bactero ... | 2011 | 21376823 |
| rrna-based profiling of bacteria in the axilla of healthy males suggests right-left asymmetry in bacterial activity. | the activity of the human armpit microbiota triggers the formation of body odor. we used differential 16s rrna gene (rdna)- and rrna-based terminal-restriction fragment length polymorphism fingerprinting in combination with cloning and sequencing to identify active members of the human armpit microbiota. dna and rna were isolated from skin scrub samples taken from both armpits of 10 preconditioned, healthy males. the fingerprint profiles indicated pronounced similarities between the armpit micro ... | 2011 | 21428987 |
| Peptoniphilus coxii sp. nov. and Peptoniphilus tyrrelliae sp. nov. isolated from human clinical infections. | Two groups of previously undescribed anaerobic, gram-positive cocci recovered from human clinical infections were characterized using phenotypic and molecular genotypic methods. Comparative genotypic analysis showed that the strains within each of these two groups were homogeneous within the group and that each group was unique within the genus Peptoniphilus. The first group is most closely related to Peptoniphilus ivorii and the second group to Peptoniphilus harei. Based on these findings we pr ... | 2011 | 22178538 |
| Peptoniphilus duerdenii sp. nov. and Peptoniphilus koenoeneniae sp. nov. isolated from human clinical specimens. | Two strains of previously unknown Gram-positive, anaerobic, coccus-shaped bacteria recovered from human wound specimens were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and biochemical characteristics, demonstrated that these organisms are genotypically and phenotypically distinct and represent previously unknown sub-lines within the order Clostridiales, within the Firmicutes. Pairwise sequence analysis demonstrated that the novel ... | 2011 | 22140159 |
| crystal structure of nad(+)-dependent peptoniphilus asaccharolyticus glutamate dehydrogenase reveals determinants of cofactor specificity. | glutamate dehydrogenases (ec 1.4.1.2-4) catalyse the oxidative deamination of l-glutamate to α-ketoglutarate using nad(p) as a cofactor. the bacterial enzymes are hexamers and each polypeptide consists of an n-terminal substrate-binding (domain i) followed by a c-terminal cofactor-binding segment (domain ii). the reaction takes place at the junction of the two domains, which move as rigid bodies and are presumed to narrow the cleft during catalysis. distinct signature sequences in the nucleotide ... | 2011 | 22068154 |
| The complex vaginal flora of West African women with bacterial vaginosis. | The spectrum of bacteria associated with bacterial vaginosis (BV) has recently expanded through taxonomic changes and the use of molecular methods. These methods have yet to be used in large-scale epidemiological studies in Africa where BV is highly prevalent. | 2011 | 21949860 |
| mistaken identity of peptoniphilus asaccharolyticus. | | 2011 | 21248090 |
| antimicrobial susceptibility of clinically relevant gram-positive anaerobic cocci collected over a three-year period in the netherlands. | the susceptibility of 14 species of 115 gram-positive anaerobic cocci (gpac) was determined for 14 antibiotics. to assure correct identification, strains were genotypically identified by fluorescence in situ hybridization and sequencing. susceptibility differences (mic₅₀ and mic₉₀) for penicillin g, clindamycin, tigecycline, levofloxacin, amoxicillin-clavulanic acid, cefoxitin, ertapenem, meropenem, metronidazole, and doxycycline were found for the three clinically most relevant gpac species: fi ... | 2010 | 21189338 |
| diagnosis of intramammary infection in samples yielding negative results or minor pathogens in conventional bacterial culturing. | up to half of quarter milk samples submitted for mastitis diagnosis are culture-negative results or lead to identification of coagulase-negative staphylococci or corynebacterium bovis in conventional culturing, the so-called minor pathogens. the interpretation and usefulness of these results in terms of udder and animal health management is limited, even though the amount of resources spent is relatively high. this work aimed to test two methods of analysis of milk samples with the goal of incre ... | 2010 | 21134309 |