| in vitro efficiency of intra- and extracellular immunization with mouse anti-ygnnv antibody against yellow grouper nervous necrosis virus. | mouse monoclonal antibody (mab-18) against yellow grouper nervous necrosis virus (ygnnv) coat protein was developed and assayed for its neutralization ability. in the present study, we cloned and sequenced the cdnas encoding heavy (gamma) and light (kappa) chains by constructing a cdna library of the mab-18 hybridoma. three expression vectors, pcmv-nnv-18h (gamma), pcmv-nnv-18l (kappa), and pcmv-nnv-18hl (both gamma and kappa chains) were constructed and successfully expressed in grouper brain ( ... | 2002 | 12163274 |
| establishment of cell lines from a tropical grouper, epinephelus awoara (temminck & schlegel), and their susceptibility to grouper irido- and nodaviruses. | four tropical marine fish cell lines have been established from the eye, fin, heart and swim bladder of grouper, epinephelus awoara (temminck & schlegel). optimum media and temperature conditions for maximum growth were standardized. the eye and swim bladder cells were mostly epithelial, but the fin and heart cells were mostly fibroblastic. the viability of cells was 95% after 1 year of storage in liquid nitrogen (-196 degrees c). besides these four cell lines, previously established grouper bra ... | 2003 | 12962209 |
| inhibition of nervous necrosis virus propagation by fish mx proteins. | mx proteins are interferon induced, antiviral proteins, expressed in response to treatment with double stranded rna or virus infection. here we report the cloning, sequencing, and antiviral property of three forms of mx genes, mxi, mxii, and mxiii from grouper (epinephelus coioides). multiple comparison of grouper mx amino acid sequences revealed the conservation of mx putative gtp-binding domain, dynamin family signature, and leucine zipper motif. we have established a new cell line, grouper br ... | 2006 | 17069756 |