| involvement of tlr21 in baculovirus-induced interleukin-12 gene expression in avian macrophage-like cell line hd11. | our previous work showed that baculovirus (antheraea pernyi nuclear polyhedrosis virus [apnpv]) induced a strong innate immunity and protected chicken from a lethal challenge of bronchitis virus. the purpose of present study was to determine the chicken toll-like receptors (tlrs) used by bv-infected immune cells to induce this immune response. we first investigated the expression of the toll-like receptor (tlr) genes in resting and bv-infected hd11 (chicken macrophage-like cell line) and dt40 (c ... | 2010 | 20471186 |
| functional analysis of the inhibitor of apoptosis genes in antheraea pernyi nucleopolyhedrovirus. | the inhibitor of apoptosis proteins (iap) plays an important role in cell apoptosis. we cloned two novel iap family members, ap-iap1 and ap-iap2, from antheraea pernyi nucleopolyhedrovirus (apnpv) genome. ap-iap1 contains two baculoviral iap repeat (bir) domains followed by a ring domain, but ap-iap2 has only one bir domain and ring. the result of transient expression in spodoptera frugiperda (sf21) showed that ap-iap1 blocked cell apoptosis induced by actinomycin d treatment and also rescued th ... | 2010 | 20437152 |
| baculovirus up-regulates antiviral systems and induces protection against infectious bronchitis virus challenge in neonatal chicken. | in this study, the antheraea pernyi nuclear polyhedrosis virus (apnpv), a member of the baculovirus family, is evaluated for its stimulation of chicken peripheral blood mononuclear cells (pbmc) and macrophage cell line hd 11 in vitro, and protection against infectious bronchitis virus (ibv) in neonatal chickens in vivo. this study showed that apnpv significantly enhanced inflammatory cytokine mrna expression in chicken pbmc and hd 11 cells through the clathrin-dependent endocytic and endosomal m ... | 2008 | 18707025 |
| complete sequence and organization of antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. | the completion and reporting of baculovirus genomes is extremely important as it advances our understanding of gene function and evolution. due to the large number of viral genomes now sequenced it is very important that authors present significantly detailed analyses to advance the understanding of the viral genomes. however, there is no report of the antheraea pernyi nucleopolyhedrovirus (anpenpv) genome. | 2007 | 17650316 |
| characterization of antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of autographa californica nucleopolyhedrovirus orf108. | antheraea pernyi nucleopolyhedrovirus (apnpv) p11 gene is 309 bp long, potentially encoding 102 amino acids with a predicted molecular weight of 11.2 kda. apnpv p11 gene was cloned into the prokaryotic expression vector pqe-30 and p11 was expressed in e. coli m15. polyclonal antiserum was made against 6xhis tagged p11 protein expressed in e. coli m15. p11 gene transcription was detected as early as 36 h post-infection (p.i.) in tussah pupa and remain at high level up to 96 h p.i. structural loca ... | 2007 | 17072759 |
| cloning and sequence analysis of the antheraea pernyi nucleopolyhedrovirus gp64 gene. | frequent outbreaks of the purulence disease of chinese oak silkworm are reported in middle and northeast china. the disease is produced by the pathogen antheraea pernyi nucleopolyhedrovirus (anpenpv). to obtain molecular information of the virus, the polyhedra of anpenpv were purified and characterized. the genomic dna of anpenpv was extracted and digested with hindiii. the genome size of anpenpv is estimated at 128 kb. based on the analysis of dna fragments digested with hindiii, 23 fragments w ... | 2005 | 16388134 |
| genome mapping and gene analysis of antheraea pernyi nucleopolyhedrovirus for improvement of baculovirus expression vector system. | we have constructed a genome dna map of the antheraea pernyi nucleopolyhedrovirus (anpenpv) and used it to identify target genes for deletion in order to improve the newly developed baculovirus expression vector system. initially, 50 independent psti fragments of viral dna were obtained by shotgun cloning, and both termini of each cloned fragment were sequenced. then, the sequence data were used for homology search against both nucleotide and amino acid sequences of other npvs in databases. this ... | 2002 | 16233185 |