| immunological properties of the fibrinolytic enzyme (fibrolase) from southern copperhead (agkistrodon contortrix contortrix) venom and its purification by immunoaffinity chromatography. | an antibody to the fibrinolytic enzyme in southern copperhead venom was produced by immunizing rabbits with chromatographically purified enzyme. the antibody was purified from rabbit blood by ammonium sulfate fractionation and protein-a affinity chromatography. the purified antibody reacted only with the fibrinolytic enzyme in southern copperhead venom as demonstrated by immunodiffusion and immunoelectrophoresis. western immunoblotting revealed that several snake venoms, including agkistrodon pi ... | 1991 | 1926169 |
| isolation of two hemorrhagic toxins from crotalus basiliscus basiliscus (mexican west coast rattlesnake) venom and their effect on blood clotting and complement. | 1. two hemorrhagic toxins of mol. wt 27,000 (b1) and 27,500 (b2) and pi 9.8 and 5.2 respectively were isolated from crotalus basiliscus venom. 2. the two proteinases did not cross-react antigenically. 3. both toxins caused hemorrhage in mice and each was capable of hydrolyzing hide power azure, casein, collagen and fibrin. 4. b1 hydrolyzed the a alpha, b beta and gamma chains of fibrinogen. b2 hydrolyzed the a alpha and b beta chains of fibrinogen, but not the gamma chain. 5. both proteinases in ... | 1990 | 2110084 |
| malarial parasites of the "jesu cristo" lizard basiliscus basiliscus (iguanidae) in panama. | | 1972 | 5008852 |
| parallopharynxs pp. (trematoda: digenea: plagiorchioidea) in iguanian lizards from the area de conservación guanacaste, guanacaste, costa rica, including parallopharynx matternae n. sp. in chaetodon basiliscus (squamata: iguania: corytophanidae). | we report 3 species of the digenean genus parallopharynx, 1 previously undescribed, from the area de conservación guanacaste (acg) in northwestern costa rica. parallopharynx gonzalezi, which was originally described in basiliscus sp. and ctenosoura similis from central costa rica, inhabits c. quinquecarinata; p. jonesi, originally described in anolis lionotus (syn. norops oxylophus) from nicaragua, inhabits n. oxylophus, n. biporcatus, and basiliscus basiliscus; and the new species, which inhabi ... | 2004 | 15165060 |
| fibrinolytic enzymes from the venoms of agkistrodon contortrix contortrix and crotalus basiliscus basiliscus: cleavage site specificity towards the alpha-chain of fibrin. | zinc metalloproteinases with fibrinolytic activity have been isolated from the venom of agkistrodon contortrix contortrix (fibrolase) and crotalus basiliscus basiliscus (basiliscusfibrases 1, 2 and 3). fibrolase cleaves the a alpha-chain of fibrinogen initially at a single site: lys413-leu. basiliscusfibrases 1, 2 and 3 also cleave at this site as well as others. since cleavage in lys-leu locations is not common among zinc metalloproteinases, we examined the degree to which the lys-leu bond dete ... | 1994 | 8085237 |
| comparison of two methods for proteolytic enzyme detection in snake venom. | an acrylamide gel system containing fibrinogen was used to detect proteolytic enzymes in snake venoms. proteolytic activity was observed as a clear area on a blue background after electrophoresis and overnight incubation in tris buffer, prior to staining with coomassie blue. venoms from eastern and western diamondback and west coast mexican rattlesnakes, crotalus adamanteus, c. atrox and c. basiliscus basiliscus, respectively, and southern copperhead, agkistrodon contortrix contortrix, were anal ... | 1986 | 3087029 |
| scanning electron microscope studies of the auditory papillae of some iguanid lizards. | the papillae basilares of 16 species (10 general) of iguanid lizards were studied by scanning electron microscopy. variations in the surface structures of the auditory papillae showed the following major differences: 1)papillae with localization of the unidirectional hair cells at the apical end of the papilla (anolis carolinensis); 2)papillae with absence or loss of a portion of the apical bidirectional hair-cell segment (basiliscus basiliscus); 3)papillae with a central, short ciliated, unidir ... | 1981 | 7304475 |
| size-dependence of water-running ability in basilisk lizards (basiliscus basiliscus) | in the past, it has been proposed that basilisk lizards (basiliscus basiliscus) are able to reach high population densities because the juveniles and adults have differing water-running abilities and therefore live in different habitats. however, there is no a priori reason to expect juveniles to be better able to run on water than adults. to determine the causal relationship between body size and water-running ability, we made three types of measurements: (1) direct morphological measurements o ... | 1996 | 9320547 |
| biochemical characterization of basilase, a fibrinolytic enzyme from crotalus basiliscus basiliscus. | snake venoms, especially from the crotalidae family, contain a variety of enzymes that prevent blood coagulation by virtue of their fibrinolytic enzymes. nineteen snake venoms were screened for fibrinolytic activity and the highest activity was found in the venom of crotalus basiliscus basiliscus venom. the active principle, basilase, was isolated, purified, and found to have fibrinolytic and fibrinogenolytic activity. it had a molecular weight of 22,000 and 1 mol of zinc per mole of protein ass ... | 1995 | 7893151 |
| purification, characterization, and fibrinogen cleavage sites of three fibrinolytic enzymes from the venom of crotalus basiliscus basiliscus. | three distinct fibrinolytic enzymes have been purified from the venom of crotalus basiliscus basiliscus (the mexican west coast rattlesnake). the high-performance liquid chromatography-based purification comprised the following steps: (a) hydrophobic interaction chromatography; (b) hydroxylapatite chromatography; (c) anion-exchange chromatography. following hydrophobic interaction chromatography two fibrinolytic activity peaks were detected, cbfib1 and cbfib2. cbfib2 was rendered homogeneous fol ... | 1992 | 1581310 |