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filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with orn-d-glu-type peptidoglycan.a spore-forming, halophilic bacterium was isolated from surface sediment located on the beach of palaeochori bay near to a shallow water hydrothermal vent area, milos, greece. the bacterium, designated sh 714t, consisted of motile, strictly aerobic rods which contained an orn-d-glu type murein and a g+c content of 35 mol%. thin sections showed a cell wall typical for gram-positive bacteria; the peptidoglycan layer, however, was very thin. the gram-reaction of the organism was negative. comparati ...200111324591
tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in xin-jiang, china.two gram-positive, rod-shaped, spore-forming and moderately halophilic bacteria (strains 28-1t, 28-4), isolated from a soil sample from a neutral salt lake in xin-jiang, china, were characterized polyphasically. on the basis of fasta (ungapped) analyses of 16s rrna gene sequences, strains 28-1t and 28-4 were shown to belong to the bacillaceae and to be closely related to filobacillus milensis dsm 13259t (97.0 %) and bacillus haloalkaliphilus dsm 5271t (95.7 %). 16s rrna gene sequence similarity ...200515653860
pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic gram-positive bacterium from a solar saltern in korea.three moderately halophilic, spore-forming strains, designated bh030062t, bh030049 and bh030080, were isolated from a solar saltern in korea. phylogenetic analyses and comparative 16s rrna gene sequence studies revealed that the isolates represent a novel distinct monophyletic lineage within the phyletic group classically defined as the genus bacillus and are most closely related to members of the genera gracilibacillus (93.7-95.1 % similarity), virgibacillus (93.5-94.8 %), halobacillus (94.8-95 ...200515653871
purification and characterization of serine proteinase from a halophilic bacterium, filobacillus sp. rf2-5.in order to find a unique proteinase, proteinase-producing bacteria were screened from fish sauce in thailand. an isolated moderately halophilic bacterium was classified and named filobacillus sp. rf2-5. the molecular weight of the purified enzyme was estimated to be 49 kda. the enzyme showed the highest activity at 60 degrees c and ph 10-11 under 10% nacl, and was highly stable in the presence of about 25% nacl. the activity was strongly inhibited by phenylmethane sulfonyl fluoride (pmsf), chym ...200515665465
reclassification of bacillus haloalkaliphilus fritze 1996 as alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in china.a spore-forming, halophilic bacterium, designated strain bh163(t), was isolated from a salt lake in china. cells were motile, strictly aerobic rods that contained type a1gamma peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. the isolate showed gram- and catalase-positive reactions and formed a terminal endospore with a swollen sporangium. the major cellular fatty acids were anteiso-c(15:0), iso-c(15:0), anteiso-c(17:0) and iso-c(16:0). the genomic dna g+c content of th ...200516166684
endospores of halophilic bacteria of the family bacillaceae isolated from non-saline japanese soil may be transported by kosa event (asian dust storm).generally, extremophiles have been deemed to survive in the extreme environments to which they had adapted to grow. recently many extremophiles have been isolated from places where they are not expected to grow. alkaliphilic microorganisms have been isolated from acidic soil samples with ph 4.0, and thermophiles have been isolated from samples of low temperature. numerous moderately halophilic microorganisms, defined as those that grow optimally in media containing 0.5-2.5 molar (3-15%) nacl, an ...200516242015
piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in thailand.a gram-positive, spore-forming and moderately halophilic bacterium was isolated from fermented fish (pla-ra) in thailand. cells of the isolate, rbu1-1(t), were strictly aerobic, motile rods and contained meso-diaminopimelic acid in the cell-wall peptidoglycan. menaquinone with seven isoprene units (mk-7) was the predominant quinone. this isolate grew at 15-48 degrees c, ph 5-9 and in 2-30 % nacl (optimally 10-20 %). the major cellular fatty acids were iso-c(15 : 0) and anteiso-c(15 : 0). polar l ...200717625167
aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family bacillaceae isolated from a saline lake.a novel moderately halophilic, non-motile, rod-shaped bacterium was isolated from a saline lake, lake shangmatala, in the inner mongolia autonomous region, china. this bacterium, designated sh4st, was strictly aerobic, catalase-positive and oxidase-negative. it grew at salinities of 3-20 % (w/v) nacl, with an optimum at 10 % (w/v) nacl. the cell-wall peptidoglycan was of the a4beta type, based on l-orn-d-asp, and the major quinone was a menaquinone with seven isoprene units (mk-7). the major fat ...200818676481
piscibacillus halophilus sp. nov., a moderately halophilic bacterium from a hypersaline iranian lake.a gram-positive, moderately halophilic bacterium, designated strain hs224(t), was isolated from the hypersaline lake howz-soltan in iran. cells of strain hs224(t) were rod-shaped, motile and produced oval endospores. phylogenetic analyses based on 16s rrna gene sequences showed that strain hs224(t) was affiliated to the genus piscibacillus, exhibiting 98.5 % sequence similarity to the type strain of piscibacillus salipiscarius. strain hs224(t) was also related closely to the type strains of aqui ...200919643892
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