| sequence analysis of the complete genome of an iridovirus isolated from the tiger frog. | we have isolated a tiger frog virus (tfv) from diseased tiger frogs, rana tigrina rugulosa. the genome was a linear double-stranded dna of 105,057 basepairs in length with a base composition of 55.01% g+c. about 105 open reading frames were identified with coding capacities for polypeptides ranging from 40 to 1294 amino acids. computer-assisted analyses of the deduced amino acid sequences revealed that 39 of 105 putative gene products showed significant homology to functionally characterized pro ... | 2002 | 11878922 |
| genomic sequence of a ranavirus (family iridoviridae) associated with salamander mortalities in north america. | disease is among the suspected causes of amphibian population declines, and an iridovirus and a chytrid fungus are the primary pathogens associated with amphibian mortalities. ambystoma tigrinum virus (atv) and a closely related strain, regina ranavirus (rrv), are implicated in salamander die-offs in arizona and canada, respectively. we report the complete sequence of the atv genome and partial sequence of the rrv genome. sequence analysis of the atv/rrv genomes showed marked similarity to other ... | 2003 | 14599794 |
| inhibition of reporter gene and iridovirus-tiger frog virus in fish cell by rna interference. | we describe the specific silencing of reporter gene lacz in fhm cells (muscle cells of fathead minnow, a fish cell line) by either expressing small hairpin rnas (shrnas) from plasmids or transfecting small interfering rnas (sirnas) transcribed in vitro. two types of dsrnas could inhibit reporter gene expression, and sirnas were more effective, while both of them worked very well in hela cells. sirnas were tested for silencing expression of the major capsid protein (mcp) encoded by tiger frog vir ... | 2005 | 15932766 |
| a broadly applicable method to characterize large dna viruses and adenoviruses based on the dna polymerase gene. | many viral pathogens are poorly characterized, are difficult to culture or reagents are lacking for confirmatory diagnoses. we have developed and tested a robust assay for detecting and characterizing large dna viruses and adenoviruses. the assay is based on the use of degenerate pcr to target a gene common to these viruses, the dna polymerase, and sequencing the products. | 2006 | 16608526 |
| identification of two novel membrane proteins from the tiger frog virus (tfv). | the tiger frog virus (tfv) belongs to the genus ranavirus in the family iridoviridae, and its genome was completely sequenced in 2002. in order to better understand the viral structure and functional genes involved in infection and virus-host interactions, two candidate genes, orf001l and orf020r, were selected for our study. orf001l and orf020r were analyzed by genomic comparison and by using the tmhmm software. both genes were conserved in the genus ranavirus, may encode putative membrane prot ... | 2008 | 18538433 |
| development of an rt-pcr detection method for mud crab reovirus. | mud crab reovirus (mcrv) causes high mortality in the cultivated mud crab. to control better an outbreak of this virus, a rapid, specific and sensitive detection method based on rt-pcr was developed. the mcrv detection method was designed based on the one-step and two-step rt-pcr which resulted in the amplification of predicted products of 433 and 304 bp. the method is specific as no cross-reaction was observed between grass carp hemorrhage virus (gchv), white spot syndrome virus (wssv), tiger f ... | 2008 | 18572256 |
| tiger frog virus can infect zebrafish cells for studying up- or down-regulated genes by proteomics approach. | tiger frog virus (tfv), a member of the iridovirus family, causes high mortality of cultured tiger frog tadpoles in southern china. to better understand tfv infection and its interaction with host cells, zebrafish embryonic fibroblast (zf4) cells, a stable polyploid cell line with most clear genetic map, was used for our present study. our results showed that tfv caused typical lytic plaque forming cytopathic effect (cpe) and that various stages of viral proliferation were observed using electro ... | 2009 | 19409947 |
| complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of iridoviridae. | soft-shelled turtle iridovirus (stiv) is the causative agent of severe systemic diseases in cultured soft-shelled turtles (trionyx sinensis). to our knowledge, the only molecular information available on stiv mainly concerns the highly conserved stiv major capsid protein. the complete sequence of the stiv genome is not yet available. therefore, determining the genome sequence of stiv and providing a detailed bioinformatic analysis of its genome content and evolution status will facilitate furthe ... | 2009 | 19439104 |
| development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of lymphocystis disease virus. | a loop-mediated isothermal amplification (lamp) assay was developed for the detection of lymphocystis disease virus (lcdv). a set of five specific primers, two inner and two outer primers and a loop primer, were designed on the basis of the major capsid protein gene of lcdv. the reaction time and temperatures were optimized for 60 min at 63 degrees c, respectively. lamp amplification products were detected by a ladder-like appearance on agarose gel electrophoresis or a naked-eye inspection of a ... | 2010 | 19891986 |
| cloning, characterization, and biological function analysis of the sidt2 gene from siniperca chuatsi. | the systemic rna interference defective protein (sid)-1 plays an important role in dsrna uptake in cells. we identified the scsidt2 gene from the mandarin fish (siniperca chuatsi), which is the first-studied sid-1 homolog in fish. scsidt2 mrna is 3380 bp long and contains a 2568-bp open reading frame that encodes an 855-amino-acid protein with an n-terminal signal peptide and ten putative transmembrane domains. tissue distribution profile in healthy fish and expression profiles of scsidt2 in inf ... | 2011 | 21334374 |
| tiger frog virus (an iridovirus) entry into hepg2 cells via a ph-dependent, atypical caveola-mediated endocytosis pathway. | tiger frog virus (tfv), in the genus ranavirus of the family iridoviridae, causes high mortality of cultured tiger frog tadpoles in china. to explore the cellular entry mechanism of tfv, hepg2 cells were treated with drugs that inhibit the main endocytic pathways. we observed that tfv entry was inhibited by nh(4)cl, chloroquine, and bafilomycin, which all can elevate the ph of acidic organelles. in contrast, tfv entry was not influenced by chlorpromazine or overexpression of a dominant-negative ... | 2011 | 21543502 |
| selected emerging diseases of amphibia. | this review summarizes the most recent updates on emerging infectious diseases of amphibia. a brief summary of batrachochytrium dendrobatidis history, epidemiology, pathogenesis, life cycle, diagnosis, treatment, and biosecurity is provided. ambystoma tigrinum virus, common midwife toad virus, frog virus 3, rana grylio virus, rana catesbeiana ranavirus, mahaffey road virus, rana esculenta virus, bohle iridovirus, and tiger frog virus ranaviruses are extensively reviewed. emerging bacterial patho ... | 2013 | 23642863 |
| differential autophagic effects triggered by five different vertebrate iridoviruses in a common, highly permissive mandarinfish fry (mff-1) cell model. | autophagy of five vertebrate iridoviruses, including one megalocytivirus (infectious spleen and kidney necrosis virus, isknv) and four ranaviruses (chinese giant salamander iridovirus, cgsiv; tiger frog virus, tfv; grouper iridovirus, giv; and largemouth bass virus, lmbv) were investigated in a common, highly permissive mandarinfish fry (mff-1) cell model. the results showed marked autophagosome formation in giv- and lmbv-infected cells but not in isknv-, cgsiv- and tfv-infected mff-1 cells. str ... | 2016 | 26748344 |
| budding of tiger frog virus (an iridovirus) from hepg2 cells via three ways recruits the escrt pathway. | the cellular endosomal sorting complex required for transport (escrt) pathway is a multifunctional pathway involved in cell physiological activities. while the majority of rna viruses bearing l-domains are known to hijack the escrt pathway to complete the budding process, the budding of large and complex enveloped dna viruses, especially iridoviruses, has been rarely investigated. in the present study, we use the tiger frog virus (tfv) as a model to investigate whether iridoviruses are released ... | 2016 | 27225426 |
| identification and differential expression analysis of micrornas encoded by tiger frog virus in cross-species infection in vitro. | tiger frog virus (tfv), dsdna virus of the genus ranavirus and family iridoviridae, causes a high mortality of tiger frog tadpoles cultured in southern china. micrornas (mirnas) have been identified in many viruses especially dna viruses such as singapore grouper iridoviruses (sgiv). micrornas play important roles in regulating gene expression for virus subsistence in host. considering that tfv infects cells of different species under laboratory conditions, we aim to identify the specific and es ... | 2016 | 27129448 |
| tiger frog virus orf080l protein interacts with litaf and impairs egf-induced egfr degradation. | tiger frog virus (tfv) belongs to the genus ranavirus, family iridoviridae, and causes severe mortality in commercial cultures in china. tfv orf080l is a gene homolog of lipopolysaccharide-induced tnf-α factor (litaf), which is a regulator in endosome-to-lysosome trafficking through its function in the endosomal sorting complex required for transport machinery. the characteristics and biological roles of tfv orf080l were identified. tfv orf080l was predicted to encode an 84-amino acid peptide (v ... | 2016 | 26956473 |
| caveolae restrict tiger frog virus release in hepg2 cells and caveolae-associated proteins incorporated into virus particles. | caveolae are flask-shaped invaginations of the plasma membrane. caveolae play important roles in the process of viruses entry into host cells, but the roles of caveolae at the late stage of virus infection were not completely understood. tiger frog virus (tfv) has been isolated from the diseased tadpoles of the frog, rana tigrina rugulosa, and causes high mortality of tiger frog tadpoles cultured in southern china. in the present study, the roles of caveolae at the late stage of tfv infection we ... | 2016 | 26887868 |
| genome-wide analyses of proliferation-important genes of iridovirus-tiger frog virus by rnai. | tiger frog virus (tfv), a species of genus ranavirus in the family iridoviridae, is a nuclear cytoplasmic large dna virus that infects aquatic vertebrates such as tiger frog (rana tigrina rugulosa) and chinese soft-shelled turtle (trionyx sinensis). based on the available genome sequences of tfv, the well-developed rna interference (rnai) technique, and the reliable cell line for infection model, we decided to analyze the functional importance of all predicted genes. firstly, a relative quantita ... | 2014 | 24886972 |
| roles of extracellular matrix components in tiger frog virus attachment to fathead minnow (pimephales promelas) cells. | tiger frog virus (tfv) belongs to the genus ranavirus (family iridoviridae) and causes significant harm in cultured frogs, resulting in substantial losses in ecological and economic field in southern china. attachment is the first step in viral life cycle, which is dependent on the interactions of virions with extracellular matrix (ecm) components. studying this process will help in understanding virus infection and controlling viral diseases. in this study, the roles of primary ecm components i ... | 2020 | 32976972 |
| rapid diagnosis of largemouth bass ranavirus in fish samples using the loop-mediated isothermal amplification method. | largemouth bass ranavirus (lmbv) has been recognized as the causative pathogen responsible for infectious skin ulcerative syndrome in cultured largemouth bass in china. a fast and simple lmbv detection method is urgently needed. here, a loop-mediated isothermal amplification (lamp) assay was established for the detection of this virus using primers targeting the major capsid protein gene of lmbv. the amplification conditions were optimized; the assay was specific for the diagnosis of lmbv, as th ... | 2020 | 32268179 |
| tiger frog virus orf104r interacts with cellular vdac2 to inhibit cell apoptosis. | ranaviruses belong to the family iridoviridae, and have become a serious threat to both farmed and natural populations of fish and amphibians. previous reports showed that ranaviruses could encode viral bcl-2 family-like proteins (vbcl-2), which play a critical role in the regulation of cell apoptosis. however, the mechanism of ranaviruses vbcl-2 interactions with host protein in mediating apoptosis remains unknown. tiger frog virus (tfv) belonging to the genus ranavirus has been isolated from i ... | 2019 | 31299465 |
| protein a from orange-spotted grouper nervous necrosis virus triggers type i interferon production in fish cell. | family nodaviridae consists of two genera: alphanodavirus and betanodavirus, and the latter is classified into four genotypes, including red-spotted grouper nervous necrosis virus, tiger puffer nervous necrosis virus, striped jack nervous necrosis virus, and barfin flounder nervous necrosis virus. type i interferons (ifns) play a central role in the innate immune system and antiviral responses, and the interactions between ifn and nnv have been investigated in this study. we have found that the ... | 2018 | 29733958 |