| phylogenetic analyses of klebsiella species delineate klebsiella and raoultella gen. nov., with description of raoultella ornithinolytica comb. nov., raoultella terrigena comb. nov. and raoultella planticola comb. nov. | the phylogenetic relationships of the type strains of 9 klebsiella species and 20 species from 11 genera of the family enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16s rrna and rpob genes. the sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. the phylogenetic inference of the sequence comparison confirmed that the genus klebsiella is heterogeneous and composed of species which form three clusters that ... | 2001 | 11411716 |
| regulation of raoultella terrigena comb.nov. phytase expression. | phytases catalyze the release of phosphate from phytate (myo-inositol hexakisphosphate) to inositol polyphosphates. raoultella terrigena comb.nov. phytase activity is known to increase markedly after cells reach the stationary phase. in this study, phytase activity measurements made on single batch cultures indicated that specific enzyme activity was subject to catabolite repression. cyclic amp (camp) showed a positive effect in expression during exponential growth and a negative effect during s ... | 2002 | 11888166 |
| klebsiella pneumoniae produces no histamine: raoultella planticola and raoultella ornithinolytica strains are histamine producers. | histamine fish poisoning is caused by histamine-producing bacteria (hpb). klebsiella pneumoniae and klebsiella oxytoca are the best-known hpb in fish. however, 22 strains of hpb from fish first identified as k. pneumoniae or k. oxytoca by commercialized systems were later correctly identified as raoultella planticola (formerly klebsiella planticola) by additional tests. similarly, five strains of raoultella ornithinolytica (formerly klebsiella ornithinolytica) were isolated from fish as new hpb. ... | 2002 | 12089029 |
| enterobacterial repetitive intergenic consensus 1r pcr assay for detection of raoultella sp. isolates among strains identified as klebsiella oxytoca in the clinical laboratory. | the enterobacterial repetitive intergenic consensus 1r pcr method, which provided recognizable profiles for reference strains of the three species of raoultella and the two genetic groups of klebsiella oxytoca, was applied to 19 clinical isolates identified as k. oxytoca. by this method, as confirmed by species-specific gene sequencing, two raoultella ornithinolytica and two unclassifiable k. oxytoca isolates were identified. | 2003 | 12682174 |
| recognition of two genetic groups in the klebsiella oxytoca taxon on the basis of chromosomal beta-lactamase and housekeeping gene sequences as well as eric-1 r pcr typing. | whilst searching for a molecular method to identify the different species of raoultella and klebsiella oxytoca, it was observed that the oxy-1 and oxy-2 beta-lactamase-producing k. oxytoca isolates displayed two distinguishable enterobacterial repetitive intergenic consensus (eric)-1r profiles. it was hypothesized that the two groups of chromosomal beta-lactamases might correspond to two groups of strains in the k. oxytoca taxon. to confirm this hypothesis, clinical isolates and reference strain ... | 2003 | 12807183 |
| genetic and biochemical characterization of the chromosomal class a beta-lactamases of raoultella (formerly klebsiella) planticola and raoultella ornithinolytica. | enterobacterial strains of raoultella spp. display a penicillinase-related beta-lactam resistance pattern suggesting the presence of a chromosomal bla gene. from whole-cell dna of raoultella planticola strain atcc 33531(t) and raoultella ornithinolytica strain atcc 31898(t), bla genes were cloned and expressed into escherichia coli. each gene encoded an ambler class a beta-lactamase, named pla-1 and orn-1 for r. planticola and r. ornithinolytica, respectively. these beta-lactamases (291 amino ac ... | 2004 | 14693555 |
| photobacterium phosphoreum caused a histamine fish poisoning incident. | an incident of histamine fish poisoning (hfp) occurred due to the consumption of iwashi maruboshi (dried sardine) in osaka, japan in march 2002. a histamine-producing bacterial strain, ys4-7, was isolated from iwashi maruboshi that contained 1700 mg of histamine per kilogram. this strain was identified as photobacterium phosphoreum by biochemical examinations and partial sequencing of 16s rdna. p. phosphoreum ys4-7 showed greater capability as a histamine producer at 4 and 12 degrees c than morg ... | 2004 | 15033270 |
| histidine decarboxylase in enterobacteriaceae revisited. | with a modification of taylor's decarboxylation broth, histidine decarboxylase was detected in enterobacter aerogenes, morganella morganii, raoultella ornithinolytica, and some strains of citrobacter youngae and raoultella planticola. this method provides a useful confirmatory test for identification of e. aerogenes strains. | 2004 | 15583342 |
| enhancement of styrene removal by pseudomonas sp. sr-5 in mixed culture with a benzoic acid-degrading bacterium in biofilter. | the growth of a styrene-degrading bacterium, pseudomonas sp. sr-5, was inhibited by benzoic acid (ba), one of the styrene degradation intermediates, in liquid culture. a benzoic acid-degrading microorganism, raoultella sp. strain a, was isolated from a peat biofilter inoculated with a wastewater. the styrene removal efficiencies of the two laboratory-scale biofilters inoculated with only strain sr-5 and a mixed culture of strains sr-5 and a were compared using a mixed packing material of peat an ... | 2005 | 16128393 |
| phylogeny of the enterobacteriaceae based on genes encoding elongation factor tu and f-atpase beta-subunit. | the phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor tu gene (tuf) and of their f-atpase beta-subunit gene (atpd). an 884 bp fragment for tuf and an 884 or 871 bp fragment for atpd were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. the atpd sequence analysis exhibited an indel specific to pantoea and tatumella species, showing, for the first time, a tight phylogenetic ... | 2005 | 16166704 |
| colanic acid is an exopolysaccharide common to many enterobacteria isolated from paper-machine slimes. | in this study, polysaccharide-producing bacteria were isolated from slimes collected from two finnish and one spanish paper mill and the exopolysaccharides (epss) produced by 18 isolates were characterised. most of the isolates, selected on the bases of slimy colony morphology, were members of the family enterobacteriaceae most frequently belonging to the genera enterobacter and klebsiella including raoultella. all of the epss analysed showed the presence of charged groups in the form of uronic ... | 2006 | 16418870 |
| confirmation of e. coli among other thermotolerant coliform bacteria in paper mill effluents, wood chips screening rejects and paper sludges. | paper sludges are solid wastes material generated from the paper production, which have been characterized for their chemical contents. some are rich in wood fiber and are a good carbon source, for example the primary and de-inking paper sludges. others are made rich in nitrogen and phosphorus by pressing the activated sludge, resulting from the biological water treatments, with the primary sludge, yielding the combined paper sludge. still, in the absence of sanitary effluents very few studies h ... | 2006 | 16714043 |
| transformation of 2,4,6-trinitrotoluene (tnt) by raoultella terrigena. | manufacture of nitroorganic explosives generates toxic wastes leading to contamination of soils and waters, especially groundwater. for that reason bacteria living in environments highly contaminated with 2,4,6-trinitrotoluene (tnt) and other nitroorganic compounds were investigated for their capacity for tnt degradation. one isolate, raoultella terrigena strain hb, removed tnt at concentrations between 10 and 100 mg l(-1) completely from culture supernatants under optimum aerobic conditions wit ... | 2007 | 16758276 |
| [raoultella planticola, a new strain degrading 2,4,5-trichlorophenoxyacetic acid]. | a new strain that degrades the herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) was isolated from soil, which was exposed to factors related to the petrochemical industry. according to its physiological, biochemical, cultural, and morphological traits, together with the sequence of the 16s rrna gene, the strain was identified as raoultella planticola 33-4ch. the strain could consume 2,4,5-t as a sole source of carbon and energy. the amount of 2,4,5-t in the culture medium decreased by 51% a ... | 2006 | 16878544 |
| phylogenetic analysis of enteric species of the family enterobacteriaceae using the oric-locus. | phylogenetic analysis of 38 enteric species belonging to the enterobacteraceae family was carried out using the non-coding locus oric, the chromosomal replication origin. the oric loci were amplified with conserved oligonucleotides and the pcr fragments were sequenced directly. the results establish a phylogenetic tree for the classification of different species of the genera escherichia, shigella, salmonella, enterobacter, citrobacter, klebsiella, raoultella, kluyvera, cedecea and buttiauxella. ... | 2007 | 16904857 |
| identification of clinical isolates of indole-positive and indole-negative klebsiella spp. | biochemical methods employed to classify bacterial species have limitations and may have contributed to the taxonomic complexity recently reported for the genus klebsiella. the objective of the present study was to apply a simple biochemical test panel to classify a collection of human klebsiella isolates. we found that with only three additional tests, it is possible to place most isolates in a defined species. analysis of a 512-bp sequence of the rpob gene was used as the reference. a total of ... | 2006 | 16928968 |
| enhancement of styrene removal efficiency in biofilter by mixed cultures of pseudomonas sp. sr-5. | the styrene-degrading bacterium pseudomonas sp. sr-5 exhibited a high styrene removability in a biofilter. however, the styrene removal efficiency (re) of sr-5 decreased with time. we carried out styrene gas removal in a biofilter inoculated with mixed cultures of sr-5 and other microorganisms to determine the possibility of obtaining an enhanced re for a long period. the following three inocula were carried out: (i) styrene-degrading bacteria, strains 1 and 3, (ii) a benzoic acid-degrading bact ... | 2006 | 16952837 |
| fluorescence in situ hybridisation detection of lactobacillus plantarum group on olives to be used in natural fermentations. | at present there are very few studies on the bacterial diversity of olives and on the importance of the microbial species for the fermentation of olives aimed to table olives production. most of the authors report on the occurrence of lactobacillus plantarum as principal member of these communities or at least as the species responsible for the fermentation. in this study, fluorescence in situ hybridisation (fish) with 16s rrna probes was used to evaluate the occurrence of l. plantarum in olives ... | 2006 | 17052794 |
| occurrence and diversity of integrons and beta-lactamase genes among ampicillin-resistant isolates from estuarine waters. | the aim of the present study was to assess the occurrence and molecular diversity of beta-lactamase genes and integrons among gram-negative ampicillin-resistant bacteria from ria de aveiro. ampicillin-resistant isolates were selected and subjected to genotyping using rep-pcr. representatives from each rep pattern were affiliated with the following taxa by sequencing the 16s rrna gene: aeromonas caviae, a. hydrophila, a. media, a. molluscorum, a. veronii, a. salmonicida, aeromonas sp., pseudomona ... | 2006 | 17125975 |
| tnt transformation products are affected by the growth conditions of raoultella terrigena. | high concentrations of 2,4,6-trinitrotoluene (tnt) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. the bacterium, raoultella terrigena strain hb, isolated from a contaminated site, converts tnt into the corresponding amino products. radio-hplc analysis with [(14)c]tnt identified aminodinitrotoluene, diaminonitrotoluene and azoxy-dimers as the main metabolites. transformation rate and the type of metabolites that predominated in the cultu ... | 2007 | 17136570 |
| removal of two waterborne pathogenic bacterial strains by activated carbon particles prior to and after charge modification. | waterborne diseases constitute a threat to public health despite costly treatment measures aimed at removing pathogenic microorganisms from potable water supplies. this paper compared the removal of raoultella terrigena atcc 33257 and escherichia coli atcc 25922 by negatively and positively charged types of activated carbon particles. both strains display bimodal negative zeta-potential distributions in stabilized water. carbon particles were suspended to an equivalent external geometric surface ... | 2006 | 17144313 |
| histidine decarboxylases and their role in accumulation of histamine in tuna and dried saury. | histamine-producing bacteria (hpb) such as photobacterium phosphoreum and raoultella planticola possess histidine decarboxylase (hdc), which converts histidine into histamine. histamine fish poisoning (hfp) is attributable to the ingestion of fish containing high levels of histamine produced by hpb. because freezing greatly decreases the histamine-producing ability of hpb, especially of p. phosphoreum, it has been speculated that hfp is caused by hdc itself from hpb cells autolyzing during froze ... | 2007 | 17220267 |
| benzoic acid-degrading bacteria from the intestinal tract of macrotermes michaelseni sjöstedt. | the intestinal tracts of termites host a wide variety of microbial symbionts, which have been implicated in degradative processes. in this study, a fungus-cultivating termite, macrotermes michaelseni was found to harbor 2.2 x 10(6) bacterial cells per ml of gut homogenates capable of degrading benzoic acid. two benzoic acid degrading bacteria were isolated from the highest dilution of gut homogenates in oxic media with benzoic acid as the sole carbon source. isolate cbc was related to stenotroph ... | 2007 | 17304624 |
| a case of severe pancreatitis complicated by raoultella planticola infection. | a 45-year-old-male presented with severe pancreatitis. two bacterial isolates obtained from peritoneal fluid and abdominal purulent secretion were identified to the species level by 15 biochemical tests and four supplementary tests as raoultella planticola. identification was confirmed by rpob gene sequencing. r. planticola is difficult to identify in the clinical laboratory, and the clinical significance of this isolation remains uncharacterized. this is the first report of pancreatitis with a ... | 2007 | 17446297 |
| the o-specific polysaccharide structure from the lipopolysaccharide of the gram-negative bacterium raoultella terrigena. | the structure of the repeating unit of the o-specific polysaccharide from the lipopolysaccharide of the enterobacterium raoultella terrigena was determined by means of chemical and spectroscopical methods and was found to be a linear tetrasaccharide containing a cyclic acetal of pyruvic acid (pyr) as depicted below.[carbohydrate structure: see text]. | 2007 | 17509546 |
| adhesion and viability of waterborne pathogens on p-dadmac coatings. | the attachment of waterborne pathogens onto surfaces can be increased by coating the surfaces with positive charge-enhancing polymers. in this paper, the increased efficacy of polydiallyldimethylammonium chloride (p-dadmac) coatings on glass was evaluated in a parallel plate flow chamber with the use of waterborne pathogens (raoultella terrigena, escherichia coli, and brevundimonas diminuta). p-dadmac coatings strongly compensated the highly negative charges on the glass surface and even yielded ... | 2008 | 17570712 |
| isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, helixpomatia and cornu aspersum (gastropoda: pulmonata). | the intestinal microbiota of the edible snails cornu aspersum fsyn: h. aspersa), and helix pomatia were investigated by culture-based methods, 16s rrna sequence analyses and phenotypic characterisations. the study was carried out on aestivating snails and two populations of h. pomatia were considered. the cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) cfu g -1, but the swedish h. pomatia appeared significantly less colonised, suggesting a higher sensitivity ... | 2007 | 17657348 |
| molecular epidemiology of two klebsiella pneumoniae mastitis outbreaks on a dairy farm in new york state. | klebsiella spp. have become an important cause of clinical mastitis in dairy cows in new york state. we describe the occurrence of two klebsiella mastitis outbreaks on a single dairy farm. klebsiella isolates from milk, feces, and environmental sources were compared using random amplified polymorphic dna (rapd)-pcr typing. the first mastitis outbreak was caused by a single strain of klebsiella pneumoniae, rapd type a, which was detected in milk from eight cows. rapd type a was also isolated from ... | 2007 | 17928424 |
| fluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills. | slime formation is a serious problem nowadays in the paper industry. some enterobacteria are associated with the formation of slime deposits in paper and board mills. detection and characterization of slime forming bacteria, belonging to the genus enterobacter, raoultella, and klebsiella have been achieved by fluorescence in situ hybridization (fish), using one probe based on the enterobacterial repetitive intergenic consensus sequence and other two rrna targeted oligonucleotide probes. the effe ... | 2008 | 18247026 |
| influence of adhesion to activated carbon particles on the viability of waterborne pathogenic bacteria under flow. | in rural areas around the world, people often rely on water filtration plants using activated carbon particles for safe water supply. depending on the carbon surface, adhering microorganisms die or grow to form a biofilm. assays to assess the efficacy of activated carbons in bacterial removal do not allow direct observation of bacterial adhesion and the determination of viability. here we propose to use a parallel plate flow chamber with carbon particles attached to the bottom plate to study bac ... | 2008 | 18351669 |
| interaction forces between waterborne bacteria and activated carbon particles. | activated carbons remove waterborne bacteria from potable water systems through attractive lifshitz-van der waals forces despite electrostatic repulsion between negatively charged cells and carbon surfaces. in this paper we quantify the interaction forces between bacteria with negatively and positively charged, mesoporous wood-based carbons, as well as with a microporous coconut carbon. to this end, we glued carbon particles to the cantilever of an atomic force microscope and measured the intera ... | 2008 | 18405910 |
| coexistence of antibiotic-producing and antibiotic-sensitive bacteria in biofilms is mediated by resistant bacteria. | antibiotic-sensitive bacteria have been found to coexist with antibiotic-producing bacteria in biofilms, but little is known about how the former develop in such an environment. here we isolated pyocyanin-sensitive bacteria belonging to the genus brevibacillus from a biofilm derived from soil extract and based on the preestablished biofilm of a pyocyanin producer, pseudomonas aeruginosa strain p1. in addition, pyocyanin-resistant strains belonging to the genus raoultella were isolated from the s ... | 2008 | 18441106 |
| proteolytic activity in stored aerobic granular sludge and structural integrity. | aerobic granules lose stability during storage. the goal of this work was to highlight the main cause of stability loss for stored granules as intracellular protein hydrolysis. the quantity of extracellular proteins was noted to be significantly lower during granule storage, and protease enzyme activities were correspondingly higher in the cores of stored granules. the proteolytic bacteria, which secrete highly active protease enzymes, were for the first time isolated and characterized by analyz ... | 2009 | 18614356 |
| how to identify raoultella spp. including r. ornithinolytica isolates negative for ornithine decarboxylase? the reliability of the chromosomal bla gene. | although raoultella planticola and raoultella ornithinolytica were described more than 20 years ago, identifying them remains difficult. the reliability of the chromosomal bla gene for this identification was evaluated in comparison with that of the 16s rdna and rpob genes in 35 raoultella strains from different origins. of the 26 strains previously identified as r. planticola by biochemical tests alone or in association with molecular methods, 21 harboured a bla gene with 99.8% identity with th ... | 2008 | 18694786 |
| co-metabolic degradation of dimethoate by raoultella sp. x1. | a bacterium raoultella sp. x1, based on its 16s rrna gene sequence, was isolated. characteristics regarding the bacterial morphology, physiology, and genetics were investigated with an electron microscopy and conventional microbiological techniques. although the isolate grew and degraded dimethoate poorly when the chemical was used as a sole carbon and energy source, it was able to remove up to 75% of dimethoate via co-metabolism. with a response surface methodology, we optimized carbon, nitroge ... | 2009 | 18989739 |
| enteric fever-like syndrome caused by raoultella ornithinolytica (klebsiella ornithinolytica). | | 2009 | 19144809 |
| [giant renal cyst as cause of colic obstruction]. | a 97-year old woman presented with a 2-months history of asthenia, loss of appetite, nauseas and intermittent diarrhea. she presented an important colic distension associated to an inflammatory syndrome. microbiological documentation (blood, urine, saddles stool) was negative. abdominal computed tomography (ct) showed a 15 cm of diameter (giant) right cortical renal cyst with colic obstruction by external colic compression. a percutaneous cyst drainage was performed allowing the evacuation of 1, ... | 2009 | 19517907 |
| neonatal enteral feeding tubes as loci for colonisation by members of the enterobacteriaceae. | the objective of this study was to determine whether neonatal nasogastric enteral feeding tubes are colonised by the opportunistic pathogen cronobacter spp. (enterobacter sakazakii) and other enterobacteriaceae, and whether their presence was influenced by the feeding regime. | 2009 | 19723318 |
| first descriptions of blakpc in raoultella spp. (r. planticola and r. ornithinolytica): report from the sentry antimicrobial surveillance program. | two strains of raoultella planticola and one of raoultella ornithinolytica showing carbapenem resistance were recovered from patients hospitalized in new jersey and ohio. all patients had received previous antimicrobial treatment, including carbapenems. these strains harbored bla(kpc-2) and bla(kpc-3). carbapenemase genes were embedded in isoforms of tn4401 and were plasmidic and chromosomal in location. | 2009 | 19812278 |
| antimicrobial substances produced by coliform strains active against foodborne pathogens. | in the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in rio de janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to nine different antibiotics. two strains (6.4%) exhibited inhibitory activity against the indicator strains, escherichia coli lmifrj and salmonella ente ... | 2010 | 19895262 |
| metabolism of (-)-epigallocatechin gallate by rat intestinal flora. | anaerobic metabolism of (-)-epigallocatechin gallate (egcg) by rat intestinal bacteria was investigated in vitro. first, intestinal bacteria which are capable of hydrolyzing egcg to (-)-epigallocatechin (egc) and gallic acid (2) were screened with 169 strains of enteric bacteria. as a result, enterobacter aerogenes, raoultella planticola, klebsiella pneumoniae susp. pneumoniae, and bifidobacterium longum subsp. infantis were found to hydrolyze egcg. subsequent steps of egcg metabolism are degrad ... | 2010 | 20043675 |
| antiserum against raoultella terrigena atcc 33257 identifies a large number of raoultella and klebsiella clinical isolates as serotype o12. | raoultella terrigena atcc 33257, recently reclassified from the genus klebsiella, is a drinking water isolate and belongs to a large group of non-typeable klebsiella and raoultella strains. using an o-antiserum against a capsule-deficient mutant of this strain, we could show a high prevalence (10.5%) of the r. terrigena o-serotype among non-typeable, clinical klebsiella and raoultella isolates. we observed a strong serological cross-reaction with the k. pneumoniae o12 reference strain, indicatin ... | 2010 | 20053705 |
| raoultella ornithinolytica bacteremia in an infant with visceral heterotaxy. | a case of raoultella ornithinolytica bacteremia in an infant with visceral heterotaxy is reported. physical examination was remarkable for markedly red skin flushing, not unlike that seen during histamine fish poisoning. r. ornithinolytica is a histamine-producing bacterium recently elucidated as a major cause of histamine fish poisoning. only 2 other cases of human infection by r. ornithinolytica have been reported. | 2010 | 20072080 |
| exopolysaccharide production by nitrogen-fixing bacteria within nodules of medicago plants exposed to chronic radiation in the chernobyl exclusion zone. | nitrogen-fixing bacteria isolated from root nodules of medicago plants growing in the 10 km zone around the chernobyl nuclear power plant were screened for the production of new water-soluble acidic exopolysaccharides (epss). the different strains belonged to the enteriobacteriaceae family (enterobacter ludwigii, raoultella terrigena, klebsiella oxytoca), except for one which belonged to the rhizobiaceae family (sinorhizobium meliloti). all of the bacteria produced highly viscous eps with an ave ... | 2010 | 20080178 |
| diversity of culturable microorganisms and occurrence of listeria monocytogenes and salmonella spp. in tuber aestivum and tuber melanosporum ascocarps. | the aim of this study was to investigate the total mesophilic microorganisms, pseudomonas genus, enterobacteriaceae family, mold and yeast counts and the presence of listeria monocytogenes and salmonella spp on tuber aestivum and tuber melanosporum ascocarps. the results confirmed that the major percentage of the microorganisms, approximately 9.0 log ufc/g, were present in the peridium, the glebas of healthy truffles being practically free of microorganisms. the predominant microbial group was t ... | 2010 | 20141948 |
| development of molecular-based methods for determination of high histamine producing bacteria in fish. | histamine (or scombroid) fish poisoning is a significant cause of food borne disease in the united states. in this study, we describe the development of a molecular-based technique which uses digoxigenin (dig) labeled dna probes for the detection of gram negative bacteria producing high amounts of histamine (>1000 ppm). a cocktail of pcr amplification fragments corresponding to a 709 bp fragment of the histidine decarboxylase (hdc) gene of four high producing bacteria (morganella morganii, enter ... | 2010 | 20392504 |
| [identifying bacteria using a matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometer. comparison with routine methods used in clinical microbiology laboratories]. | the methods routinely used for bacterial identification in clinical microbiology laboratory, although miniaturized and automated, are still based on the same basic principles as classical identification methods. nevertheless, technological advances are emerging which could modify these routine methods. we report a comparative study between conventional identification methods and mass spectrometry maldi-tof (ms maldi-tof) for bacterial identification in the clinical microbiology laboratory. | 2010 | 20409613 |
| structural investigation of an exopolysaccharide substituted with a lactyl ether group produced by raoultella terrigena ez-555-6 isolated in the chernobyl exclusion zone. | raoultella terrigena strain ez-555-6, isolated from a root nodule of medicago sativa harvested in the chernobyl exclusion zone, produces a non-referenced high-molecular-mass exopolysaccharide (eps). the structure of this eps was determined using a combination approach including monosaccharide composition (glc-fid, hpaec-pad), determination of glycosylation sites (glc-eims) and 1d/2d nmr ((1)h, (13)c) and esims (hr, ms/ms) studies of oligosaccharides obtained from mild acid hydrolysis. the eps wa ... | 2010 | 20417501 |
| gut bacterium of dendrobaena veneta (annelida: oligochaeta) possesses antimycobacterial activity. | the new bacterial strain with antimycobacterial activity has been isolated from the midgut of dendrobaena veneta (annelida). biochemical and molecular characterization of isolates from 18 individuals identified all as raoultella ornithinolytica genus with 99% similarity. the bacterium is a possible symbiont of the earthworm d. veneta. the isolated microorganism has shown the activity against four strains of fast-growing mycobacteria: mycobacterium butiricum, mycobacterium jucho, mycobacterium sm ... | 2010 | 20452357 |
| performance of a sulfidogenic bioreactor and bacterial community shifts under different alkalinity levels. | the performance of a sulfidogenic bioreactor and the response of bacterial populations to influent alkalinity changes were investigated. the bioreactor reached 40% of sulfate removal efficiency (sre) with 0 mg l(-1) of alkalinity, and single-stranded conformation polymorphism profiles showed that some members of bacteroides, dysgonomonas, sporobacter, quinella, and citrobacter became dominant populations. 16s rrna gene library analysis indicated that the actinobacteria group increased from 0% in ... | 2010 | 20688512 |
| a rare case of soft-tissue infection caused by raoultella planticola. | raoultella species are gram-negative, non-motile bacilli primarily considered to be environmental bacteria. raoultella planticola is a rare cause of human infections. we report a case of serious soft-tissue infection in a young male tiler who presented with cellulitis of his left thumb. he had sustained a crush injury to his left thumb 10 days earlier in a soiled environment. he noted a minor break in the skin and he washed the wound out with running water. one week later, he experienced pain, e ... | 2010 | 20811592 |
| molecular diagnosis of raoultella planticola infection of a surgical site. | raoultella planticola has been rarely diagnosed in clinical specimens. a case of a polymicrobial surgical site infection primarily caused by r. planticola in a 66-year-old caucasian male with a fractured left tibia after an open reduction internal fixation of his left ankle is described and confirms this organism to be an opportunistic human pathogen. this pathogen was diagnosed with rapid clinical molecular pathogen diagnostic methods, which allowed an appropriate therapy to be implemented, the ... | 2010 | 20852504 |
| comparison of maldi tof with conventional identification of clinically relevant bacteria. | a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory. | 2010 | 20924806 |
| description of gibbsiella quercinecans gen. nov., sp. nov., associated with acute oak decline. | gram-negative, facultatively anaerobic bacterial strains were consistently isolated from oak trees displaying symptoms of extensive stem bleeding. in britain, this disorder is called acute oak decline (aod). a similar condition has been noted on species of mediterranean oak in spain. the identity of bacterial isolates from symptomatic trees in both countries was investigated using molecular techniques and phenotypic assays. 16s rrna gene sequencing indicated that the strains were most closely re ... | 2010 | 21115313 |
| comparison of (gtg)5-oligonucleotide and ribosomal intergenic transcribed spacer (its)-pcr for molecular typing of klebsiella isolates. | molecular typing of klebsiella species has become important for monitoring dissemination of β-lactamase-producers in hospital environments. the present study was designed to evaluate poly-trinucleotide (gtg)(5)- and rdna intergenic transcribed spacer (its)-pcr fingerprint analysis for typing of klebsiella pneumoniae and klebsiella oxytoca isolates. multiple displacement amplified dna derived from 19 k. pneumoniae (some with an esbl-phenotype), 35 k. oxytoca isolates, five k. pneumoniae, two k. o ... | 2010 | 21129413 |
| evaluation of molecular techniques for identification and enumeration of raoultella terrigena atcc 33257 in water purifier efficacy testing. | raoultella terrigena atcc 33257, a representative of the coliform group, is commonly used as a challenge organism in water purifier efficacy testing. in addition to being time consuming, traditional culturing techniques and metabolic identification systems (including automated systems) also fail to accurately differentiate this organism from its closely related neighbors belonging to the enterobacteriaceae group. molecular-based techniques, such as real-time quantitative polymerase chain reactio ... | 2010 | 21132347 |
| evaluation of three phenotypic identification systems for clinical isolates of raoultella ornithinolytica. | raoultella spp. have recently been separated from the genus klebsiella based on their molecular characteristics. it was discovered that raoultella ornithinolytica can be misidentified as klebsiella oxytoca by commonly used phenotypic identification systems. therefore, this study evaluated the ability of three phenotypic systems to identify r. ornithinolytica compared with the genotypic methods sequence-specific primer pcr (ssp-pcr), 16s rrna gene sequence analysis using the microseq 500 system16 ... | 2011 | 21212145 |
| a rare human infection of raoultella ornithinolytica in a diabetic foot lesion. | | 2011 | 21245606 |
| sources of klebsiella and raoultella species on dairy farms: be careful where you walk. | klebsiella spp. are a common cause of mastitis, milk loss, and culling on dairy farms. control of klebsiella mastitis is largely based on prevention of exposure of the udder to the pathogen. to identify critical control points for mastitis prevention, potential klebsiella sources and transmission cycles in the farm environment were investigated, including oro-fecal transmission, transmission via the indoor environment, and transmission via the outdoor environment. a total of 305 samples was coll ... | 2011 | 21257074 |
| spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. | the spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. the proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) and dna sequencing in order to relate them to the performance and stability of the bioelectrochemical system. the reproducibility o ... | 2011 | 21400098 |
| sepsis caused by raoultella terrigena. | | 2011 | 21731819 |
| quantification of total and specific gram-negative histamine-producing bacteria species in fish using an mpn real-time pcr method. | quantification of histamine-producing bacteria (hpb) is necessary in order to elucidate the role that hpb play in scombrotoxin (histamine) fish poisoning. we report here the evaluation of a real-time pcr method for the quantification of total and specific gram-negative hpb species in fish using a most probable number (mpn) format. the species-specific real-time pcr assay was 100% inclusive for independently detecting morganella morganii, enterobacter aerogenes, raoultella planticola/ornithinolyt ... | 2011 | 21839377 |
| green fluorescent protein (gfp)-labeling of enterobacteria associated with fruit flies (diptera: tephritidae) and persistence in their natural host rhagoletis completa cresson. | fruit flies (diptera: tephritidae) are a highly successful, widespread group of insects that cause economic damage in agriculture. data available so far on the composition of the bacterial community associated with their digestive tract indicate that members of enterobacteriaceae are the species most often isolated. bacteria naturally occurring in insect guts may be engineered and used to study the spatial and functional interactions of microbes within the insect system and offer one route to me ... | 2011 | 22004066 |
| cholangitis with septic shock caused by raoultella planticola. | raoultella planticola is a rare isolate in human infection. this is a case of cholangitis complicated with septic shock caused by r. planticola. | 2011 | 22096135 |
| isolation and identification of a new tetrodotoxin-producing bacterial species, raoultella terrigena, from hong kong marine puffer fish takifugu niphobles. | puffer fish, takifugu niphobles, collected from the hong kong coastal waters were screened for tetrodotoxin-producing bacteria. a gram-negative, non-acid-fast, non-sporing and rod shaped bacterial strain (designated as gutb01) was isolated from the intestine of the puffer fish and was shown to produce tetrodotoxin (ttx). based on the microbial identification (midi) and 16s-23s rdna internal transcribed spacer (its) phylogenetic analysis, the strain was identified as raoultella terrigena. the ttx ... | 2011 | 22163191 |
| encapsulation of r. planticola rs-2 from alginate-starch-bentonite and its controlled release and swelling behavior under simulated soil conditions. | the plant growth-promoting bacteria (pgpr) raoultella planticola rs-2 was encapsulated with the various blends of alginate, starch, and bentonite for development of controlled-release formulations. the stability and release characteristics of these different capsule formulations were evaluated. the entrapment efficiency of rs-2 in the beads (capsules) was more than 99%. the diameter of dry beads ranged from 0.98 to 1.41 mm. the bacteria release efficiency, swelling ratio, and biodegradability of ... | 2011 | 21879356 |
| Raoultella infection causing fever of unknown origin. | | 2011 | 22105425 |
| antibacterial effect of food additives and detergents against histamine-producing bacteria on food contact material surfaces. | we investigated the antibacterial activity of food additives and detergents against histamine-producing bacteria on food contact material surfaces. based on minimum inhibitory concentration (mic) testing with morganella morganii nbrc3848, raoultella planticola nbrc3317 and enterobacter aerogenes nctc10006, we screened nine food additives and four detergents with relatively high inhibitory potency. we prepared food contact material surfaces contaminated with histamine-producing bacteria, and dipp ... | 2011 | 22200801 |
| gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities. | dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ... | 2012 | 22234511 |
| the role of nutrients in the biodegradation of 2,4,6-trinitrotoluene in liquid and soil. | the widely used explosive 2,4,6-trinitrotoluene (tnt) has residues that are potentially explosive, toxic, and mutagenic. tnt and other explosives can be degraded by microorganisms; however, biostimulation is needed for process efficiency. to investigate the effectiveness of using biostimulation to degrade tnt, we added varying concentrations of a nutrient amendment consisting of inorganic salts, plant extracts, and molasses to soil and liquid media. for the inoculum we used a consortium of bacte ... | 2012 | 22245864 |