| an experimental, nadph-diaphorase histochemical and immunocytochemical study of mesocestoides vogae tetrathyridia. | in order to test the role of nitric oxide in flatworms, mesocestoides vogae tetrathyridia were incubated together with l-arginine, which is the substrate for nitric oxide synthesis, or with ng-nitro-l-arginine, which is an irreversible inhibitor of nitric oxide synthase. normally, tetrathyridia attach to each other with the aid of their suckers, forming clusters. the rate of cluster formation was followed during the incubations. l-arginine stimulated, and ng-nitro-l-arginine clearly inhibited, t ... | 1999 | 10404277 |
| in vitro taurocholate-induced segmentation and clustering of mesocestoides vogae (syn. corti) tetrathyridia (cestoda)--inhibition byh cestocidal drugs. | mesocestoides vogae (syn. m. corti) tetrathyridia were cultured in the presence of sodium taurocholate, for the purpose of exploring the suitability of this organism for the in vitro assay of cestocidal drugs. parasite clustering and segmentation were observed as taurocholate-dependent effects in biphasic and monophasic media, respectively. interestingly, representative members of two major classes of known cestocidal agents (namely, albendazole and praziquantel) blocked these effects. furthermo ... | 2001 | 11355676 |
| in vivo response of mesocestoides vogae to human insulin. | summary: successful host invasion by parasitic helminths involves detection and appropriate response to a range of host-derived signals. insulin signal response pathways are ancient and highly-conserved throughout the metazoans. however, very little is known about helminth insulin signalling and the potential role it may play in host-parasite interactions. the response of mesocestoides vogae (cestoda: cyclophyllidea) larvae to human insulin was investigated, focusing on tyrosine-phosphorylation ... | 2009 | 19079819 |
| case report: first confirmed case of canine peritoneal larval cestodiasis caused by mesocestoides vogae (syn. m. corti) in japan. | canine peritoneal larval cestodiasis (cplc) is an unusual parasitic disease in dogs that is caused by asexual proliferation of larval mesocestoides. a 12 year-old spayed shetland sheepdog with abdominal distension was referred to the animal medical center at nihon university, japan. the presence of ascites was confirmed by abdominal ultrasonography and x-ray imaging. in addition, a number of parasites were observed in the ascitic fluid collected by abdominal paracentesis. each of the whitish col ... | 2014 | 24560366 |
| case report: protein-losing enteropathy caused by mesocestoides vogae (syn. m. corti) in a dog. | an eight-year-old, neutered, female shetland sheepdog presented with a 6-week history of small intestinal diarrhea. regenerative anemia, hypoproteinemia, and an increased plasma c-reactive protein concentration were detected on blood examination. fecal examination and abdominal radiography were unremarkable. abdominal ultrasonography showed diffusely hyperechoic mucosa in the small intestine. gastroduodenoscopy, performed under general anesthesia, revealed mucosal edema and increased granularity ... | 2014 | 25129334 |
| in vitro culture of mesocestoides corti metacestodes and isolation of immunomodulatory excretory-secretory products. | cestode-mediated diseases hold the interesting feature of persisting metacestode larvae dwelling within the host tissues, in the midst of the immune response. excretory-secretory (es) products of the metacestode larval stage modulate the host immune response and modify the outcome of the disease. therefore, isolation and analysis of axenic metacestode es products are crucial to study their properties. here, we report the development of a system for long-term in vitro cultivation of the metacesto ... | 2016 | 27120409 |
| reduction of oxidative stress and liver injury following silymarin and praziquantel treatment in mice with mesocestoides vogae (cestoda) infection. | oxidative stress is a common mechanism contributing to hepatic damage and fibrogenesis in a variety of liver disorders. the liver is the target organ for many parasitic infections, hence there is a great demand for the development of novel treatment strategies. in the present study conducted on mice infected with larval stage of mesocestoides vogae, we investigated effects of therapy with praziquantel (pzq) alone and in combination with silymarin on liver gsh content, lipid peroxidation and larv ... | 2010 | 20620222 |
| dynamics of hepatic stellate cells, collagen types i and iii synthesis and gene expression of selected cytokines during hepatic fibrogenesis following mesocestoides vogae (cestoda) infection in mice. | in the present study, the relationship between progression of mesocestoides vogae infection in the liver of mice, the accumulation rate of collagen types i and iii, gene expression of fibrogenic factors and cytokines was examined within 6weeks p.i. due to asexual multiplication, the total number of larvae in the liver increased considerably and 63.4% were found in collagen capsules on day 42 p.i. intense staining for both collagens was recorded in the activated hepatic stellate cells (hscs) thro ... | 2010 | 19631650 |
| differential release and phagocytosis of tegument glycoconjugates in neurocysticercosis: implications for immune evasion strategies. | neurocysticercosis (ncc) is an infection of the central nervous system (cns) by the metacestode of the helminth taenia solium. the severity of the symptoms is associated with the intensity of the immune response. first, there is a long asymptomatic period where host immunity seems incapable of resolving the infection, followed by a chronic hypersensitivity reaction. since little is known about the initial response to this infection, a murine model using the cestode mesocestoides corti (syn. meso ... | 2008 | 18398489 |
| impact of treatment with praziquantel, silymarin and/or beta-glucan on pathophysiological markers of liver damage and fibrosis in mice infected with mesocestoides vogae (cestoda) tetrathyridia. | mesocestoides vogae tetrathyridia infection in mice causes hepatocyte injury, hepatic granulomatous inflammmation, liver fibrosis and chronic peritonitis manifested with portal hypertension. to reduce the detrimental effect of parasites on the host liver, the effect of the anthelmintic drug praziquantel (pzq) in combination with natural products silymarin (an antioxidant) and beta-glucan (an immunomodulator) was investigated. the therapeutic effect of drugs was assessed by means of aminotransfer ... | 2008 | 18394210 |
| two novel mesocestoides vogae fatty acid binding proteins--functional and evolutionary implications. | this work describes two new fatty acid binding proteins (fabps) identified in the parasite platyhelminth mesocestoides vogae (syn. corti). the corresponding polypeptide chains share 62% identical residues and overall 90% similarity according to clustalx default conditions. compared with cestoda fabps, these proteins share the highest similarity score with the taenia solium protein. m. vogae fabps are also phylogenetically related to the fabp3/fabp4 mammalian fabp subfamilies. the native proteins ... | 2008 | 18076655 |
| mucin-type o-glycosylation in mesocestoides vogae (syn. corti). | protein glycosylation is an important post-translational modification underlying host-parasite interactions, which may determine the outcome of infection. although mesocestoides vogae represents an important model for investigating the various aspects of cestode biology, virtually no information is available about the structure and synthesis of glycans in this parasite. in this work, focused on the initiation pathway of mucin-type o-glycosylation in m. vogae, we characterized o-glycoproteins bea ... | 2008 | 17892882 |
| characterization of the carcinoma-associated tk antigen in helminth parasites. | expression of tk antigen, a truncated carbohydrate antigen, was examined in helmith parasites. using the monoclonal antibody lm389, this antigen was detected in extracts from taenia hydatigena, mesocestoides vogae (syn corti), and taenia crassiceps. no reactivity was observed in thysanosoma spp., dipylidium caninum, fasciola hepatica, and nyppostrongylus brasiliensis. on the basis of their electrophoretic mobility, different patterns of tk-bearing glycoproteins were observed among t. hydatigena, ... | 2007 | 17300782 |
| antibody response in mice infected with mesocestoides vogae (syn. mesocestoides corti) tetrathyridia after treatment with praziquantel and liposomised glucan. | the therapeutic effect of praziquantel (pzq) involves synergy with the humoral immune response during helminth infections, which is modulated by parasitic antigens. during experimental murine infections with the larval stage of cestoda mesocestoides vogae (syn. m. corti), dynamic changes in the igg and igm antibody serum levels to both soluble somatic and secretory larval antigens were investigated after administration of pzq alone and after its co-administration with the immunomodulator (l-->3) ... | 2007 | 17252273 |
| praziquantel and liposomized glucan-treatment modulated liver fibrogenesis and mastocytosis in mice infected with mesocestoides vogae (m. corti, cestoda) tetrathyridia. | beta-glucans are immunomodulators able to activate innate immunity and to potentiate acquired immune reactions. we investigated the impact of co-administration of liposomized beta-glucan on the larvicidal effect of the anthelmintic praziquantel (pzq) in the livers and peritoneal cavities in mice infected with mesocestoides vogae (m. corti). also, within 2 weeks following therapy (up to day 29 p.i.) we examined collagen synthesis in the livers of mice by means of biochemical determination of hydr ... | 2006 | 16556345 |
| systematics of mesocestoides (cestoda: mesocestoididae): evaluation of molecular and morphological variation among isolates. | a hypothesis-based framework was used to test if 3 genetic strains of mesocestoides (clades a, b, and c) are distinct evolutionary lineages, thereby supporting their delimitation as species. for comparative purposes, 3 established cestode species, taenia pisiformis, taenia serialis, and taenia crassiceps were assessed using the same methods. sequence data from mitochondrial rdna (12s) and the second internal transcribed spacer of nuclear rdna (its-2) revealed derived (autapomorphic) characters f ... | 2005 | 16539028 |
| praziquantel and albendazole damaging action on in vitro developing mesocestoides corti (platyhelminthes: cestoda). | parasitic flatworms present several steps of body architecture rearrangement during their fast transition from one developmental stage to another, which are, at least in part, responsible for their evasion from host immune response. besides, different developmental stages present different degrees of susceptibility to drug action, and the identification of more susceptible stages is of importance for the definition of therapeutical approaches. mesocestoides corti (syn. mesocestoides vogae) is co ... | 2006 | 16325461 |
| ecological analysis and description of the intestinal helminths present in dogs in mexico city. | the objective of the present study was to determine the prevalence of parasitoses in stray dogs from mexico city, as well as to describe the structure of the intestinal helminth communities present in said hosts. one hundred and twenty dog intestines were collected and dissected to evaluate their parasitic load; finding parasites in 102 dogs (85%). the cestodes collected were: dipylidium caninum in 72 dogs (60%), taenia hydatigena in three (2.5%), taenia pisiformis in two (1.6%), echinococcus gr ... | 2004 | 15631907 |
| pharmacological characterisation of neuropeptide f (npf)-induced effects on the motility of mesocestoides corti (syn. mesocestoides vogae) larvae. | neuropeptide f is the most abundant neuropeptide in parasitic flatworms and is analogous to vertebrate neuropeptide y. this paper examines the effects of neuropeptide f on tetrathyridia of the cestode mesocestoides vogae and provides preliminary data on the signalling mechanisms employed. neuropeptide f (>/=10 microm) had profound excitatory effects on larval motility in vitro. the effects were insensitive to high concentrations (1 mm) of the anaesthetic procaine hydrochloride suggesting extrane ... | 2004 | 14711593 |
| the toxicity of praziquantel against mesocestoides vogae (syn. corti) tetrathyridia can be assessed using a novel in vitro system. | we recently standardised mesocestoides vogae (syn. corti) tetrathyridia cultures in the presence of sodium taurocholate. parasite clustering and segmentation were observed as taurocholate-dependent effects in biphasic and monophasic media, respectively, and both were inhibited by a specific minimum inhibitory concentration (m.i.c.) of the cestocidal drugs albendazol and praziquantel. in the present study, we analysed the relationship between clustering inhibition and drug toxicity using praziqua ... | 2003 | 12658458 |
| tritiated thymidine ([3h]-tdr) and immunocytochemical tracing of cellular fate within the asexually dividing cestode mesocestoides vogae (syn. m. corti). | this report documents the presence of an active thymidine kinase (tk) system within mesocestoides vogae tetrathyridia as quantified by tritiated thymidine ([3h]-tdr) incorporation using liquid scintillation counting. a 100-fold increase in [3h]-tdr incorporation was observed at 37 degrees c when compared with its incorporation at 0 degrees c. thymidine's competitive analogue, brdu, competed for sites within newly replicated dna. immunohistochemical trials performed here using antibodies against ... | 2000 | 11085229 |
| effects of free and liposomized praziquantel on the surface morphology and motility of mesocestoides vogae tetrathyridia (syn. m. corti; cestoda: cyclophyllidea) in vitro. | the effects of in vitro exposure to praziquantel (pzq), liposomized pzq (lip.pzq), and empty liposomes on the surface morphology and motility of mesocestoides vogae tetrathyridia were investigated using scanning electron microscopy (sem) and a motility apparatus. examination of treated larvae showed an effect that was concentration- and time-dependent, involving morphological damage that was similar in character for all of the treated groups. the most marked effects were a flattening and elongat ... | 1998 | 9521013 |
| serotonin, reserpine, and motility in mesocestoides tetrathyridia. an experimental spectrofluorometry and immunocytochemistry study. | the relationship between serotonin (5-ht) and motility in tetrathyridia of mesocestoides vogae (syn. m. corti) was studied with the aid of reserpine. reserpine decreases the content of 5-ht as measured spectrofluorometrically and immunocytochemically and, furthermore, inhibits the motility, thus indicating a connection between the two. the results support the hypothesis about 5-ht being an excitatory neurotransmitter of motor activity in m. vogue. new neuroanatomical details were revealed by imm ... | 1995 | 8570584 |
| proton nuclear magnetic resonance analysis of liver metabolites from mice infected with mesocestoides vogae. | proton nmr spectra of liver extracts from mice infected with mesocestoides vogae for 24 or 133 days showed differences in the concentrations of liver metabolites when compared to those of normal liver. moderately infected livers (24 d.p.i.) had significantly less glucose and significantly more glycine, cholines, alanine and lactate than uninfected controls. similar changes in the concentrations of these metabolites were also found in mice with heavily infected livers (133 d.p.i.), and, in additi ... | 1993 | 8106188 |
| formation and excretion of calcareous bodies by the metacestode (tetrathyridium) of mesocestoides vogae. | the function of calcareous bodies, commonly found in the parenchyma of cestodes and trematodes, is relatively poorly understood. the present histochemical ultrastructural study of the proliferative tetrathyridia of mesocestoides vogae revealed that calcareous corpuscles begin to form as organic (lipid-protein) masses that coalesce in parenchymal (calcareogenic) cells. concentric accretion of organic and inorganic crystalline material then leads to the formation of typical refractile calcareous b ... | 1991 | 1865268 |
| inhibition of tapeworm thioredoxin and glutathione pathways by an oxadiazole n-oxide leads to reduced mesocestoides vogae infection burden in mice. | parasitic flatworms cause serious infectious diseases that affect humans and livestock in vast regions of the world, yet there are few effective drugs to treat them. thioredoxin glutathione reductase (tgr) is an essential enzyme for redox homeostasis in flatworm parasites and a promising pharmacological target. we purified to homogeneity and characterized the tgr from the tapeworm mesocestoides vogae (syn. m. corti). this purification revealed absence of conventional tr and gr. the glutathione r ... | 2015 | 26132905 |
| immunity and immune modulation elicited by the larval cestode mesocestoides vogae and its products. | larval cestodes (metacestodes) induce long-lasting infections leading to considerable pathology in humans and livestock. their survival is typically associated with th2-biased immune responses and immunosuppressive effects and depends on the parasite's ability to excrete/secrete antigens with immunoregulatory properties. here, mesocestoides vogae, a natural parasite of mice, is proposed as a new model species for the identification and characterization of cestode-derived immunomodulatory factors ... | 2015 | 26218296 |
| morphological and molecular characterization of post-larval pre-tetrathyridia of mesocestoides sp. (cestoda: cyclophyllidea) from ground skink, scincella lateralis (sauria: scincidae), from southeastern oklahoma, usa. | free pre-tetrathyridia of mesocestoides sp. are described, for the first time, from samples obtained from the coelomic cavity of a ground skink, scincella lateralis from oklahoma, usa. closer examination of these early stage tapeworms revealed they were transitional metamorphosis stages between a post-hexacanth procercoid form to the full metacestode of mesocestoides. a series of transitional stages from before the suckers and apical organ are developed, through full development of both structur ... | 2018 | 29400996 |
| purification of native m. vogae and h. contortus tubulin by tog affinity chromatography. | microtubules are non-covalent cylindrical polymers formed by alpha- and beta-tubulin heterodimer units, crucial for cell division, intracellular transport, motility and differentiation. this makes them very attractive pharmacological targets exploited to develop different drugs such as anthelmintics, antifungals, and antineoplastics. in this work, in order to establish an in vitro target-based screen to integrate to the search for new anthelmintics, we explored the extraction of native assembly- ... | 2017 | 28942049 |
| development of novel valerolactam-benzimidazole hybrids anthelmintic derivatives: diffusion and biotransformation studies in helminth parasites. | in the search for new anthelmintics able to overcome the resistance problem against all available drugs in livestock, the synthesis of novel valerolactam-benzimidazole hybrid compounds was reported. this allowed us to obtain these in vitro and in vivo bioactive compounds using nippostrongylus brasiliensis rat model by integrating physiology-based assays and ex vivo diffusion studies. in order to further study those novel hybrid molecules, haemonchus contortus (a sheep gastrointestinal nematode o ... | 2015 | 25816976 |
| towards an understanding of mesocestoides vogae fatty acid binding proteins' roles. | two fatty acid binding proteins, mvfabpa and mvfabpb were identified in the parasite mesocestoides vogae (platyhelmithes, cestoda). fatty acid binding proteins are small intracellular proteins whose members exhibit great diversity. proteins of this family have been identified in many organisms, of which platyhelminthes are among the most primitive. these proteins have particular relevance in flatworms since de novo synthesis of fatty acids is absent. fatty acids should be captured from the media ... | 2014 | 25347286 |